• ligand
  • The proposed method involves preparing a processed-mixture-for-screening (PMFS) with each mixture sample and an exogenous reference ligand, initiating competitive binding among ligands from the PMFS to a target immobilized on magnetic particles, recovering target-ligand complexes in equilibrium by magnetic force, extracting and concentrating bound ligands, and analyzing ligands in the PMFS and the concentrated extract by chromatography. (biomedcentral.com)
  • Ligand binding assays (LBA) is an assay, or an analytic procedure, whose procedure or method relies on the binding of ligand molecules to receptors, antibodies or other macromolecules. (wikipedia.org)
  • There are numerous types of ligand binding assays, both radioactive and non-radioactive. (wikipedia.org)
  • As such, ligand binding assays are a superset of radiobinding assays, which are the conceptual inverse of radioimmunoassays (RIA). (wikipedia.org)
  • Ligand binding assays are used primarily in pharmacology for various demands. (wikipedia.org)
  • Historically, ligand binding assay techniques were used extensively to quantify hormone or hormone receptor concentrations in plasma or in tissue. (wikipedia.org)
  • The ligand-binding assay methodology quantified the concentration of the hormone in the test material by comparing the effects of the test sample to the results of varying amounts of known protein (ligand). (wikipedia.org)
  • The foundations for which ligand binding assay have been built are a result of Karl Landsteiner, in 1945, and his work on immunization of animals through the production of antibodies for certain proteins. (wikipedia.org)
  • The first successful ligand binding assay was reported in 1960 by Rosalyn Sussman Yalow and Solomon Berson. (wikipedia.org)
  • As a direct result of these monumental findings, researchers have continued the advancement of ligand binding assays in many facets in the fields of biology, chemistry, and the like. (wikipedia.org)
  • Ligand binding assays provide a measure of the interactions that occur between two molecules, such as protein-bindings, as well as the degree of affinity (weak, strong, or no connection) for which the reactants bind together. (wikipedia.org)
  • Radioligands are used to measure the ligand binding to receptors and should ideally have high affinity, low non-specific binding, high specific activity to detect low receptor densities, and receptor specificity. (wikipedia.org)
  • reactions
  • Essential aspects of binding assays include, but are not limited to, the concentration level of reactants or products (see radioactive section), maintaining the equilibrium constant of reactants throughout the assay, and the reliability and validity of linked reactions. (wikipedia.org)
  • sample
  • In theory, an ideal approach to the combinatorial library strategy would integrate the preparation of multiple candidate ligands in each mixture as a library member with the simultaneous estimation of affinities and the concurrent judgment of binding site(s) of all candidate ligands in the mixture as a sample. (biomedcentral.com)
  • This type of analytic test can be used to test for the presence of target molecules in a sample that are known to bind to the receptor. (wikipedia.org)