• The repressor found in the phage lambda is a notable example of the level of control possible over gene expression by a very simple system. (wikidoc.org)
  • Bacteriophage (phage) Lambda (λ) has played a key historic role in driving our current understanding of molecular genetics. (uwaterloo.ca)
  • Morphology of Phage Lambda 2. (biologydiscussion.com)
  • DNA and Gene Organization of Phage Lambda 3. (biologydiscussion.com)
  • Life Cycles of Phage Lambda 4. (biologydiscussion.com)
  • Genetic Map of Phage Lambda 5. (biologydiscussion.com)
  • Phage lambda is a virus of E. coli K12 which after entering inside host cell normally does not kill it in-spite of being capable of destroying the host. (biologydiscussion.com)
  • These enzymes were found in nature in the temperate bacteriophage lambda. (igem.org)
  • Enterobacteria phage λ ( lambda phage ) is a temperate bacteriophage that infects Escherichia coli . (wikidoc.org)
  • The life cycle of lambda phages is controlled by cI and Cro proteins. (wikidoc.org)
  • The lambda phage will remain in the lysogenic state if cI proteins predominate, but will be transformed into the lytic cycle if cro proteins predominate. (wikidoc.org)
  • Bacteriophage lambda interacts with E. coli proteins. (string-db.org)
  • Like all temperate bacteriophages, lambda utilizes a lysogenic infection life cycle, wherein its genome is incorporated into the genome of the E. coli host genome, to excise itself at a later time. (igem.org)
  • Previous research had shown that the lambda phage introduced its own genetic information into that of its host, inserting it at a specific site in the host genome. (phys.org)
  • This dataset contains the 48502 base-pair-long genome of the bacteriophage lambda virus. (r-project.org)
  • Lambda phage recombination in E. coli . (igem.org)
  • We directly tested this idea by using high-throughput gene editing-phenotyping technology (MAGE-Seq) to measure the fitness landscape of a virus, bacteriophage λ, as it coevolved with its host, the bacterium Escherichia coli . (elifesciences.org)
  • It utilizes a tractable system, bacteriophage λ and E. coli , to ask questions that unite different pillars of evolutionary theory - evolutionary genetics (via the fitness landscape analogy), co-evolution, and host-parasite interactions. (elifesciences.org)
  • Isolation of a lambda transducing bacteriophage carrying the relA gene of Escherichia coli. (yale.edu)
  • Bacteriophage Lambda binds to the target E. coli cell, the tail tip binding to a maltose receptor. (wikidoc.org)
  • The bacteriophage lambda is a parasite of the intestinal bacterium Escherichia coli. (r-project.org)
  • Bacteriophage-Derived Deoxyribonucleic Acid from Innovative Research developed from purified phage whch results in the characteristic five and eight bands after digestion with EcoR I and Hind III respectively. (innov-research.com)
  • A genomic library consisting of partially digested 10 to 20 kilobase pair fragments of Treponema pallidum deoxyribonucleic acid (DNA) was constructed using bacteriophage lambda EMBL-3 as the vector. (bmj.com)
  • Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells lambda bacteriophage can also undergo a lytic cycle or can enter a lysogenic cycle, in which the page DNA is incorporated into that of the host, awaiting a signal that initiates events leading to replication of the virus and lysis of the host cell. (genomicglossaries.com)
  • The lambda repressor is a dimer also known as the cI protein . (wikidoc.org)
  • The solution structure of the Oct-1 POU-specific domain reveals a striking similarity to the bacteriophage lambda repressor DNA-binding domain. (embl.de)
  • A human dwarfism mutation that affects positive control in the related POU domain protein Pit-1 maps to the same region of the HTH motif as do positive control mutations in lambda repressor. (embl.de)
  • The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). (ucsd.edu)
  • Applying it to the bistable genetic switch in bacteriophage lambda, we find that cooperative binding between binding sites on the prophage DNA lead to non-Markovian behavior, as quantified by the conditional activity. (elsevierpure.com)
  • Shenker, JQ & Lin, MM 2015, ' Cooperativity leads to temporally-correlated fluctuations in the bacteriophage lambda genetic switch ', Frontiers in Plant Science , vol. 6, no. (elsevierpure.com)
  • The creation of recombinant DNA molecules is possible due to the use of naturally occurring restriction endonucleases (restriction enzymes), bacterial enzymes produced as a protection mechanism to cut and destroy foreign cytoplasmic DNA that is most commonly a result of bacteriophage infection. (pressbooks.pub)
  • The virus uses a trick that prevents the RNA polymerase from terminating the transcription process: It introduces 'lambda-N' (λN), a tiny protein that attaches itself to the host 's RNA polymerase and forces it to continue transcription of the viral genes . (phys.org)
  • Upon adsorption on the lamb receptor of the host cell, lambda gDNA is injected through the tail which forms a hollow tube through which the DNA passes to the cell. (biologydiscussion.com)
  • Their efforts have been focused on bacteriophages-viruses that rely on bacterial hosts for replication. (phys.org)
  • Well, this has been a challenge, especially having never worked with viruses much before - learning about bacteriophage lambda from the esteemed Noreen Murray and Dave Dryden (an acolyte of Murray's at Edinburgh University, UK, who I had the privilege of doing an unpaid summer internship with) was not much help. (pall.com)
  • Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. (wikidata.org)
  • Int cooperatively binds with IHF (which is composed of A and B subunits) in order to catalyze both the integration and excision reactions in the natural lambda phage system shown below. (igem.org)
  • Since phi 29 and lambda are both packed to a similar DNA density, it is unclear whether the six fold difference in forces is caused by a difference (phi 29= 57pN, BNID 103129 ) between the phages or a difference in the experimental conditions. (harvard.edu)
  • The POUs domain is structurally similar to the DNA-binding domains of the bacteriophage lambda and 434 repressors and 434 Cro. (embl.de)
  • Sequence specificity of mutagenesis in the cI gene of bacteriophage lambda. (nih.gov)
  • Background: The bacteriophage lambda paradigm in gene regulation revealed several basic principles of transcription regulation. (nih.gov)
  • 21. Binding properties, cell delivery, and gene transfer of adenoviral penton base displaying bacteriophage. (nih.gov)
  • 22. Gene transfer to mammalian cells using genetically targeted filamentous bacteriophage. (nih.gov)
  • 23. In vivo gene delivery and expression by bacteriophage lambda vectors. (nih.gov)
  • He was a National Institutes of Health, NIH, Postdoctoral Fellow at Harvard Medical School, where he discovered a bacteriophage lambda gene involved in lysis. (nih.gov)
  • Related vectors: lambda. (addgene.org)
  • Plasmids and bacteriophage are commonly used molecular vectors which are described as following. (microbenotes.com)
  • Over his 44-year career at the Texas A&M College of Medicine and College of Agriculture and Life Sciences(external link), Young has made broad advances in the understanding of bacteria-infecting viruses called bacteriophages, or phages. (nih.gov)
  • Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105. (lookformedical.com)
  • Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. (lookformedical.com)
  • Bacteriophage-Derived Deoxyribonucleic Acid EcoR I Fragment from Innovative Research developed by the digestion of purified lambda DNA with the restriction endonuclease EcoR I. The mixture separates into 5 identifiable bands on agarose gel electrophoresis. (innov-research.com)
  • A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE . (nih.gov)
  • Coliphage lambda proteins Rz and Rz1 are the inner membrane and outer membrane subunits of the spanin complex-a heterotetramer that bridges the periplasm and is essential for the disruption of the outer membrane during phage lysis. (tamu.edu)
  • Identification of the lambda integrase surface that interacts with Xis reveals a residue that is also critical for Int dimer formation. (nih.gov)
  • Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. (lookformedical.com)
  • The vector is isolated and used to make functional bacteriophages. (biology-pages.info)
  • Researchers at National Cancer Institute (NCI) developed an engineered bacteriophage lambda () vector for displaying antigens as a vaccine in the treatment of cancer and infectious diseases. (nih.gov)
  • a phage lambda vector with a q(-) mutation was used to enhance the expression of the lambda system. (liverpool.ac.uk)
  • Our analysis confirms that these strains still carry genetic elements such as bacteriophage lambda (λ) and the F plasmid, but also indicates that they have undergone extensive laboratory-based evolution. (nih.gov)
  • Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell. (expasy.org)
  • heterobivalent tyrosine recombinases play a prominent role in numerous bacteriophage and transposon recombination systems. (liverpool.ac.uk)
  • This technology relies on the use of modified versions of the recombinases from the bacteriophage lambda. (labster.com)
  • red/et recombination, a powerful homologous recombination system based on the red operon of lambda phage or rece/ rect from rac phage, provides an innovative approach for dna engineering. (liverpool.ac.uk)
  • The occurrence of binding site arrays distinguishes lambda attP very clearly from other DNA sequences containing single recognition sites by chance. (nih.gov)
  • Isolation and some properties of a bacteriophage infected during the fermentation of lactic acid beverage. (microbiologyresearch.org)
  • To overcome these limitations, bacteriophages were proposed for the development of more cost-effective, immunogenic vaccines. (nih.gov)
  • Fago temperado inducible y especie tipo de los virus de género similar a lambda, de la familia SIPHOVIRIDAE. (bvsalud.org)
  • In contrast to phage lambda, mEp021 virus particle production was partially restored (>1/3 relative to wild type) when nus mutants (nusA1, nusB5, nusC60, and nusE71) were infected with mEp021 and Gp17 was overexpressed. (bvsalud.org)
  • Tth DNA Ligase activity is tested in reaction with bacteriophage lambda DNA digested with SalI and SmaI, with a dilution series of ligase. (blirt.eu)
  • BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. (lookformedical.com)
  • The following year, they published the sequence of bacteriophage lambda, which has 48,502 base pairs. (latimes.com)
  • to display the aggregation-prone ligand binding domain (lbd) of the human peroxisome proliferator-activated receptor gamma (ppargamma) on the surface of bacteriophages to establish an easy screening assay for the identification of ppargamma ligands. (liverpool.ac.uk)
  • Srivastava, B.S.: Repair of gamma ray induced damage in bacteriophage lambda. (muhaz.org)
  • display of aggregation-prone ligand binding domain of human ppar gamma on surface of bacteriophage lambda. (liverpool.ac.uk)