Balb 3t3 cells. Medical search. Frequent questions

DNAs from five CdCl(2)-induced transformed cell lines were isolated and gene transfection assay was performed using NIH-3T3 cells. (cdc.gov)
Determined by a cell proliferation assay using Balb/c 3T3 cells. (peprotech.com)
A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. (degruyter.com)
The test substance is considered to cause structural chromosomal aberrations in cultured mammalian cells according to an in vitro OECD 473 chromosomal aberration assay. (europa.eu)
The test substance has a potential to induce forward mutations in mammalian cell lines (HGPRT gen locus) according to OECD 476 HGPRT assay. (europa.eu)
Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line and NRK rat normal kidney cell line. (rndsystems.com)
Burn fluid at a concentration of 1-2% is capable of accelerating cell proliferation by 2.5 times, promoting fibroblast monolayer contraction, and accelerating cell migration on the wound surface, thus stimulating cell functions necessary for successful healing, partly due to the presence of matrix metalloproteinases. (consensus.app)
The presence of burn fluid in cultivating medium accelerated cell proliferation by 2.5 times compared to normal conditions, promoted fibroblast monolayer contraction, and accelerated cell migration on the wound surface, thus stimulating cell functions necessary for successful heating. (consensus.app)
Fluorescence recovery after photobleaching (FRAP)kinetics of histone H1-zero were recorded using mouse fibroblast cell line stably expressing GFP-H1-zero. (ucsd.edu)
Proteins that act both at the cell surface and intracellularly will be discussed in this thesis, with particular emphasis on the fibroblast growth factors. (beauty104.com.tw)
Epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2) induce the proliferation of neural precursor cells isolated from specific regions of the embryonic and adult brain. (beauty104.com.tw)
Although the cytotoxicity of endodontic sealers has been extensively investigated, most previous studies have used fibroblast cell lines. (bvsalud.org)

Using viable cell membrane immunofluorescence, most established and early passage normal murine cell lines were significantly reactive with the antiserum, irrespective of neoplastic transformation, strain genotype, or whether they were of embryonic or adult tissue origin. (duke.edu)
bFGF is a critical component of human embryonic stem cell culture medium. (biofargo.com)
Comparative biochemical studies of rapamycin and wortmannin on transforming growth factor-beta 1 (TGF-beta1)-mediated G(1)-S cell cycle progression in C3H-10T1/2 embryonic fibroblasts. (janechin.net)
In primary cultures of mouse embryonic day 14 (E14) striatum, maintained in vitro for 24 hr, 12% of the cells responded to FGF-2, whereas no response to EGF could be detected. (beauty104.com.tw)

The effect of the Ttc7 fsn/fsn mutation on stromal cells was tested in a xenograft model in conjunction with transcriptomic analysis of Ttc7 fsn/fsn fibroblasts. (nih.gov)
High inductive magnetic stimuli and their effects on mesenchymal stromal cells, dendritic cells, and fibroblasts [med. (emf-portal.org)

a) Protocol for immunofluorescence with lympathic endothelial cells (LEC) using Lab-Tek II Chamber Slides. (reliatech.de)
b) Protocol for immunofluorescence with lympathic endothelial cells (LEC) and HUVECs in a cell culture dish. (reliatech.de)
EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, epithelial and endothelial cells, and promotes colony formation of epidermal cells. (akronbiotech.com)
One of the most important functions of bFGF is the promotion of endothelial cell proliferation and the physical organization of endothelial cells into tube-like structures. (beauty104.com.tw)

Studies have been performed to determine if cadmium chloride (CdCl(2)) can induce morphological cell transformation, DNA from CdCl(2)-induced transformed cells can transform other mammalian cells, and the transformed cells induced by CdCl(2) can form tumors in nude mice. (cdc.gov)
DNA from all five CdCl(2)-induced transformed cell lines tested was found to induce varying degrees of transfection-mediated transformation in NIH-3T3 cells. (cdc.gov)
These results indicate that CdCl(2) is capable of inducing morphological cell transformation and that the transformed cells induced by CdCl(2) are potentially tumorigenic. (cdc.gov)
Another test using mouse lymphoma and Balb/3T3 cells showed that povidone-iodine has no significant mutagenic or transformation capabilities. (drugs.com)

In contrast to the extensive and long-lasting changes in morphology of 3T3 cells after 0.16 microM TPA addition, only slight and shorter-lived morphological effects of TPA were observed in 3T3-E12a cells. (rupress.org)
The influence of burn fluid was assessed by morphology and specific functional activities of cells characteristic of the healing process--proliferation, monolayer contraction and migration of cells in wound model. (consensus.app)

The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL. (abcam.co.jp)
The ED₅₀ as determined by the dose-dependent stimulation of proliferation of Balb-c 3T3 cells is 5 x 10⁶ units/mg. (biovisi.com)

We sought to identify whether TTC7A mutations dysregulate epithelial cells only or whether a cell-intrinsic defect in lymphocytes or other cells contributes to disease manifestations. (nih.gov)
HB-EGF is an EGF-related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. (prosci-inc.com)
EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. (akronbiotech.com)
Akron's cGMP-compliant rHu EGF can be used to promote the activation and proliferation of fibroblasts and a number of other epithelial cells. (akronbiotech.com)

Static magnetic field enhances the anticancer efficacy of capsaicin on HepG2 cells via capsaicin receptor TRPV1 [med. (emf-portal.org)
This can either occur by cell surface receptor interactions, as in the case with peptide growth factors, or by interactions with intracellular molecules, as in the case with steroid hormones. (beauty104.com.tw)

Fresh thymocytes from BALB/cJ (GIX-), C57BL/6J (GIX-), and 129/J (GIX+) mouse strains were also reactive with Friend gp71 antiserum, and this activity, as well as that of an antiserum prepared against purified AKR gp71, were also group specific. (duke.edu)

In order to take a new approach to previous in vitro studies on the cytotoxicity of endodontic materials, it would be interesting to evaluate the response of fibroblasts and macrophages, which are cell lines involved in periapical inflammation and repair. (bvsalud.org)

The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
Profundice en los temas de investigación de 'Efecto citotóxico y genotóxico in vitro del extracto crudo y etanólico del rizoma de curcuma longa l. (edu.pe)
Priming of striatal cells for 24 hr with FGF-2 but not with EGF was sufficient to induce the appearance of EGF- and FGF-2 responsive cells after only 2 d in vitro. (beauty104.com.tw)
Thus, neural precursor cells from the mouse E14 striatum initially responding to FGF-2 only acquire EGF responsiveness later during in vitro development. (beauty104.com.tw)
The international EU/COLIPA in vitro phototoxicity validation study - results of phase II (blind trial) - Part 1 - The 3T3 NRU phototoxicity test. (medscape.com)
In spite of the advantages of the in vitro tests, they are not able to mimic the orchestrated role of cells present inperiradicular region and the long-term cytotoxicity presented by the sealers. (bvsalud.org)

We found that I. sinicola extract (ISE) increased cell proliferation in a dose-dependent manner. (degruyter.com)

Despite successful hematopoietic stem cell transplantation, ongoing progression of gastrointestinal manifestations can be life-threatening in TTC7A-deficient patients. (nih.gov)
Therefore, understanding biochemical dynamics of cell cycle progression may lead to target-specific therapy with improved side effect profiles. (janechin.net)
An obvious requirement for the stringent control of cell cycle progression is the prevention of deregulated proliferation - loss of control may result in tumors and cancers. (janechin.net)
These factors couple extracellular signals to intracellular processes to determine cell cycle progression. (janechin.net)

up cell lysates and harvesting tissue cultures from various cell culture dishes. (thomassci.com)
Infected 3T3 cultures had been gathered at 80% to 100% cytopathic impact and put through three freezeCthaw cycles. (logic2010.org)
The 6-d-old cultures also contained a small number of TuJ1-positive cells that responded to FGF-2 only. (beauty104.com.tw)

Absence of ppgpp production in synchronised balb/c mouse 3t3 cells on " by P Thammana, R R. Buerk et al. (jax.org)
Absence of ppgpp production in synchronised balb/c mouse 3t3 cells on isoleucine starvation. (jax.org)
details] Effects of Low-Intensity Electromagnetic Fields on the Proliferation and Differentiation of Cultured Mouse Bone Marrow Stromal Stem Cells [med. (emf-portal.org)

Phorbol esters and gene expression: the role of rapid changes in K+ transport in the induction of ornithine decarboxylase by 12-O-tetradecanoylphorbol-13-acetate in BALB/c 3T3 cells and a mutant cell line defective in Na+K+Cl- cotransport. (rupress.org)
A BALB/c 3T3 preadipose cell line defective in Na+K+Cl- cotransport (3T3-E12a cells) has been used to study the relationship between phorbol ester-induced rapid changes in cation fluxes and changes in expression of a gene known to be modulated by this agent. (rupress.org)
These results suggest that rapid changes in ion fluxes mediated by the Na+K+Cl- cotransport system are necessary for at least one of the phorbol ester-induced changes in gene expression in responsive cells. (rupress.org)
Modern approaches to treating cancer take advantage of critical biochemical differences between cancer cells and normal cells - from radiation therapy to chemotherapy to experimental gene therapy. (janechin.net)

FGF-6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. (peprotech.com)
The expression of the major glycoprotein, gp71, of murine leukemia virus was studied on the surfaces of a variety of normal murine cell lines with a monospecific rabbit antiserum raised against purified Friend murine leukemia virus gp71. (duke.edu)

TPA-induced changes in intracellular K+ content were diminished in 3T3-E12a cells as compared with parental cells. (rupress.org)
details] A 60 Hz uniform electromagnetic field promotes human cell proliferation by decreasing intracellular reactive oxygen species levels [med. (emf-portal.org)

Results: Crude extract produced an IC 50 of 12.98 ± 0.21 μg/mL for the HT-29 tumor cell line, which is lower than the value obtained for DU-145, with an IC 50 of 36.77 ± 9.12 μg/mL. (edu.pe)
Effect of burn exudate on functional activity of Balb/3T3 cell line and the role of matrix metalloproteinases in this process]. (consensus.app)
The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. (bvsalud.org)

details] The relationship between temperature and calcium in acute cell damage after exposure to radiofrequency or thermal energy in isolated neonatal and adult rabbit cardiac myocytes [med. (emf-portal.org)

Very good product for human stem cell and cancer stem cell maintenance and proliferation, of higher quality than similar product of competitors! (peprotech.com)
Squamous cell carcinoma may also be associated with human papillomavirus but should not be treated with Condylox 0.5% Solution. (globalrph.com)
human brain, exacerbation of encephalitis (we.e., increased existence of Compact disc4+ and Compact disc8+ T-cells), and chronic reactive phenotypes of citizen glial cells (we.e., raised MHC course II aswell as PD-L1 amounts, suffered microgliosis, and elevated glial fibrillary acidic proteins (GFAP) appearance on astrocytes) vs. neglected, infected pets. (logic2010.org)
These data show that persistent glial cell activation, unremitting post-encephalitic neuroinflammation, and its own linked long-term neurological sequelae in response to viral human brain an infection are modulated with the immunoregulatory properties of Tregs. (logic2010.org)

The mammalian cell cycle typically completes in 24 hours, where dramatic changes occur in cellular metabolism and cytoskeletal physiology. (janechin.net)
Tamoxifen acts as an anti-estrogen (inhibiting agent) in the mammary tissue, but as an estrogen (stimulating agent) in cholesterol metabolism, bone density, and cell proliferation in the ENDOMETRIUM. (lookformedical.com)

1998. Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles. (cdc.gov)

Ti 3 C 2 T x MXene Composite 3D Hydrogel Potentiates mTOR Signaling to Promote the Generation of Functional Hair Cells in Cochlea Organoids. (peprotech.com)
The morphological and functional responses of 3T3 and 3T3-E12a cells to TPA were also compared. (rupress.org)
In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. (beauty104.com.tw)

Salivary gland-passed trojan was harvested in NIH 3T3 cells for 2 passages after that, which reduced any carry-over of salivary gland tissues. (logic2010.org)
Viral share titers had been driven on 3T3 cells as 50% tissues culture infective dosages (TCID50) per milliliter. (logic2010.org)

Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma[J]. Frontiers in Immunology, 2017, 7. (kactusbio.cn)

Am J Respir Cell Mol Biol 10:253-258. (cdc.gov)
J Cell Biol (1985) 101 (6): 2316-2323. (rupress.org)

Thus, mutation of the Na+K+Cl- cotransport system renders overall potassium transport in mutant cells largely insensitive to modulation by TPA. (rupress.org)

Regulatory T-cell depletion Transgenic Foxp3-DTR-GFP mice had been used to research the consequences of severe Quinine Treg depletion on persistent neuroinflammation pursuing viral encephalitis. (logic2010.org)

Factors mandating cellular consequence include cell surface-receptors, cytoplasmic proteins, and nuclear proteins. (janechin.net)

A wide array of chemical substances can influence cell growth and differentiation. (beauty104.com.tw)
After growth factor treatment for 6 d, a population of nestin-positive cells responding to both EGF and FGF-2 were identified. (beauty104.com.tw)
Not long after FGF1 and FGF2 were isolated, another group isolated a pair of heparin -binding growth factors which they named HBGF-1 and HBGF-2, whilst a third group isolated a pair of growth factors that caused proliferation of cells in a bioassay containing blood vessel endothelium cells which they called ECGF-1 and ECGF-2. (beauty104.com.tw)

The FGFs are heparin -binding proteins and interactions with cell-surface associated heparan sulfate proteoglycans has been shown to be essential for FGF signal transduction. (beauty104.com.tw)

Macrophages are the prevalent cells in inflammatory infiltrates that respond to sealers 12-13 . (bvsalud.org)

Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. (abcam.co.jp)

Dysregulation of processes driven by these factors is a severe liability to the cell and therefore represents potential areas for therapeutic intervention. (janechin.net)

Mini Cell Scrapers for harvesting cells or cell lysates from 96-, 48- and 24-well plates. (thomassci.com)

However, the lineage relationship between the EGF- and FGF-2-responsive cells is unknown. (beauty104.com.tw)
Seventy-five percent of these FGF-2-responsive cells were beta tubulin III (TuJ1)-positive neurons, and 25% expressed nestin, a marker for neuroepithelial precursors. (beauty104.com.tw)

When cells proliferate in the absence of appropriate driving signals, cancer is the undesirable consequence. (janechin.net)

The aim of this study was to investigate the effects of Ishige sinicola , a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. (degruyter.com)

Non-transformed BALB/c-3T3 cells and cells from 10 transformed cell lines induced by CdCl(2) were injected into both axillary regions of nude mice. (cdc.gov)
All 10 CdCl(2)-induced transformed cell lines formed fibrosarcomas in nude mice within 39 days of inoculation. (cdc.gov)
The only murine cells tested not detectably expressing gp71 determinants were BALB/3T3 lines. (duke.edu)
Materials and methods: The cytotoxic effect was evaluated using DU-145, HT-29, 3T3 BALB/c cell lines. (edu.pe)

details] Effects of 940 MHz EMF on bioluminescence and oxidative response of stable luciferase producing HEK cells [med. (emf-portal.org)

Note: NK cell activation reagent Product Information Activity NK cells activated with this product have a cytotoxic index (T-C) of at least 15% Formulation Lyophilized Stability & Storage Samples are stable. (biofargo.com)

Our results reveal a previously unsuspected fundamental cell-extrinsic role of Ttc7. (nih.gov)

The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. (prosci-inc.com)
It is a highly specific chemotactic and mitogenic factor for many cell types, and appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). (biofargo.com)

Addition of fresh medium containing serum or exposure to hypoosmotic conditions resulted in the induction of ODC in both 3T3 and 3T3-E12a cells. (rupress.org)

The mammalian cell is furnished with receptors linked to interactive series of cytoplasmic networks for controlling cellular processes. (janechin.net)

In contrast to its effect on parental 3T3 cells, 12-O-tetradecanoylphorbol-13-acetate (TPA) did not inhibit either furosemide-sensitive 86Rb+ influx or the rate of 86Rb+ efflux from preloaded mutant cells. (rupress.org)

Triazoles are a class of synthetic antifungal and antiprotozoal drugs that inhibit the enzyme lanosterol 14α-demethylase, disrupting ergosterol synthesis in fungal cells. (lookformedical.com)

routinely used for cell culture. (thomassci.com)
The slim blade (15mm, 0.6") is perfect for work in 35 and 60mm Petri dishes as well as 6 and 12 well cell culture clusters. (thomassci.com)

Th17 cell may have a role in severe asthma and chronic obstructive pulmonary disease (COPD). (cdc.gov)

An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)

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