• The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase. (wikipedia.org)
  • Apoplastic ascorbate oxidases (AOs) play a critical role in reactive oxygen species (ROS)-mediated innate host immunity by regulating the apoplast redox state. (lsuhsc.edu)
  • These data show that apoplastic redox-active transition metals are involved in the ascorbate-induced [Ca2+]cyt. (deepdyve.com)
  • This mechanism may underlie ascorbate release, signalling phenomena, apoplastic redox reactions, iron acquisition, and control the ionic and electrical equilibrium (together with K+ efflux via GORK channels). (deepdyve.com)
  • iNOS expression requires NADPH oxidase-dependent redox signaling in microvascular endothelial cells. (umaryland.edu)
  • ascorbate oxidase purified from Cucurbita sp. (agrisera.com)
  • Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). (agrisera.com)
  • EPR spectroscopy measurements demonstrate that salinity (NaCl) triggers the accumulation of root apoplastic ascorbyl radicals in an A9C-dependent manner, confirming that l-ascorbate leaks through anion channels under depolarization. (deepdyve.com)
  • Moreover, we aimed to elucidate how a ROS producer, NADPH oxidase (NOX), responds to changing Mg levels. (tubitak.gov.tr)
  • The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohFwhich were grown under hydroponic conditions with 25 and 100 μM Cd for 1 and 5 days. (conicet.gov.ar)
  • This enzyme participates in ascorbate metabolism. (wikipedia.org)
  • The aim of this work was to investigate changes in isoenzyme patterns of enzymes related to reactive oxygen species (ROS) detoxification such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione-S-transferase (GST) in cotton under Mg deficiency. (tubitak.gov.tr)
  • A simple and sensitive colorimetric assay for serum diamine oxidase (DAO) activity was based on a coupled reaction with peroxidase and a new chromogen, 10-(carboxymethyl-aminocarbonyl)-3,7-bis(dimethylamino) phenothiazine sodium salt (DA-67). (fujita-hu.ac.jp)
  • SA improved the antioxidant defense in terms of ascorbate peroxidase (APX) and polyphenol oxidase (PPO) activities. (bvsalud.org)
  • and by 64.8% and 91.2%, 21.9% and 72.7%, and 76.7% and 166.7% for enzymes activity including ascorbate peroxidase, glutathione peroxidase, and phenylalanine ammonia-lyase, respectively. (bvsalud.org)
  • Cadmium increased catalase activity in WT plants and decreased it in Atrboh mutants, while glutathione reductase and glycolate oxidase activities increased in Atrboh mutants, and superoxide dismutases were down-regulated inAtrbohC. (conicet.gov.ar)
  • Laccase (EC 1.10.3.2 ) (urishiol oxidase), an enzyme found in fungi and plants, which oxidizes many different types of phenols and diamines. (expasy.org)
  • Structurally ceruloplasmin exhibits internal sequence homology, and seem to have evolved from the triplication of a copper-binding domain similar to that found in laccase and ascorbate oxidase. (expasy.org)
  • Both patterns are derived from the same region, which in ascorbate oxidase, laccase, in the third domain of ceruloplasmin, and in copA, contains five residues that are known to be involved in the binding of copper centers. (expasy.org)
  • The blue oxidases, ascorbate oxidase, laccase and ceruloplasmin. (expasy.org)
  • This family is found in flowering plants, and shows greater sequence similarity to a family of laccases (EC 1.10.3.2) from plants than to other known ascorbate oxidases. (unl.edu)
  • Members of this protein family are the copper-containing enzyme L-ascorbate oxidase (EC 1.10.3.3), also called ascorbase. (unl.edu)
  • Similarly, superoxide extracellularly generated by xanthine oxidase/xanthine yielded hydroxyl radicals. (cdc.gov)
  • Whereas a hampering effect mediated by PGP bacterial inoculation was registered on levels of superoxide anion, hydroxyl radical, electrolyte leakage, and polyphenol oxidase activity, with a decrease of 0.53%, 14.12%, 2.70%, and 5.70%, respectively, under a highest Cd level (150 mg/kg) compared with control plants. (bvsalud.org)
  • in the following order: A-A-B-A-C-C. The A-type domain is related to the multicopper oxidases. (expasy.org)
  • The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. (expasy.org)
  • 5 Since ascorbate seems to influence the free metal, and since 90-95% of copper found in the serum is bound to ceruloplasmin, some scientists have hypothesized that ascorbate (or citrate) may have an influence on this protein. (naturalcalm.ca)
  • The oxidase activity (i.e., the ability to bind free copper and iron) of ceruloplasmin has been suggested to be lower in the presence of ascorbate in vitro and in animal models. (naturalcalm.ca)
  • Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. (agrisera.com)
  • However, if doses of ascorbate are not provided to satisfy this potential draw on the nutrient, first local tissues involved in the disease, then the blood, and then the body in general become deplete of ascorbate (ANASCORBEMIA and ACUTE INDUCED SCURVY). (doctoryourself.com)
  • The deficit of ascorbate probably starts in the tissues directly involved in the disease and then spreads to other tissues of the body. (doctoryourself.com)
  • In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 ⇌ {\displaystyle \rightleftharpoons } 2 dehydroascorbate + 2 H2O Thus, the two substrates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. (wikipedia.org)
  • Intriguingly, the replacement of gluconate with ascorbate in the patch-clamp pipette reveals a large ascorbate efflux current, which shows sensitivity to the anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. (deepdyve.com)
  • In water solutions, this substance is present as a monovalent anion, ascorbate- (Asc-), which easily donates electrons to oxidized molecules and scavenges reactive oxygen species (ROS). (deepdyve.com)
  • Co-evolved Plant And Blast Fungus Ascorbate Oxidases Orchestrate The R" by Jiexiong Hu, Muxing Liu et al. (lsuhsc.edu)
  • L-ascorbate oxidase (EC 1.10.3.3 ), a higher plant enzyme. (expasy.org)
  • L-ascorbate oxidase, plant type. (unl.edu)
  • Stressful conditions of any kind greatly increase utilization of vitamin C. Ascorbate excreted in the urine drops markedly with stresses of any magnitude unless vitamin C is provided in large amounts. (doctoryourself.com)
  • Irwin Stone (10, 11, 12) pointed out the potential of vitamin C in the treatment of many diseases, the inability of humans to synthesize ascorbate, and the resultant condition hypoascorbemia. (doctoryourself.com)
  • The interaction of copper and ascorbate (i.e., vitamin C) has been described since the early 1960s. (naturalcalm.ca)
  • Copper-dependent metalloenzymes relevant to the clinical phenotype include tyrosinase (pigmentation of skin and hair), lysyl oxidase (elastin and collagen cross-linking), ascorbate oxidase (skeletal development), monoamine oxidase (possibly responsible for pili torti), superoxide dismutase (free-radical detoxification), dopamine beta-hydroxylase (catecholamine production), peptidyl-glycine alpha-amidating mono-oxygenase (bioactivation of peptide hormones), and cytochrome c oxidase (electron transport and possibly responsible for hypothermia). (medscape.com)
  • Klenner found that viral diseases could be cured by intravenous sodium ascorbate in amounts up to 200 grams per 24 hours. (doctoryourself.com)
  • The proposed method eliminates the interferences occurring in serum with use of ascorbate oxidase and stops the reaction with sodium diethyldithiocarbamate, leaving the methylene blue in the reaction mixture stable for about 2 h. (fujita-hu.ac.jp)
  • Exogenous copper and iron stimulate the ascorbate-induced [Ca2+]cyt. (deepdyve.com)
  • Dietary ascorbate has been suggested to interfere with copper absorption in several animal and human studies at high levels. (naturalcalm.ca)
  • The patient is thereby put at risk for complications of metabolic processes known to be dependent upon ascorbate. (doctoryourself.com)
  • Exogenous ascorbate also induces a moderate increase in programmed cell death symptoms in intact roots, but it does not activate Ca2+ influx currents in patch-clamped root protoplasts. (deepdyve.com)
  • If this massive draw on the small ascorbate stores of the body is not fully satisfied, the condition of ANASCORBEMIA results. (doctoryourself.com)