• Here, we contrasted the genomic landscape of cytogenetically defined aphidicolin-induced CFSs (aCFSs) to that of nonfragile sites, using multiple logistic regression. (psu.edu)
  • The adozelesin-induced RPA hyperphosphorylation can be blocked by the replicative DNA polymerase inhibitor, aphidicolin, overwatch 2 aimbot hack download that adozelesin-triggered cellular DNA damage responses require active DNA replication forks. (aquagadget.com)
  • Here we find that the replication fork progresses at 1.3kbp/min in mouse fibroblast cells, consistent with other studies, and quantify replication fork stalling by replication inhibitor aphidicolin. (lancs.ac.uk)
  • Embryos treated with aphidicolin, resulting in a halted replication fork and therefore a reduced DNA concentration, were tested for Chk1 activation and Cdc25A degradation. (vt.edu)
  • Finally, the multi-Ub conjugates of TOP1 were observed whether or not aphidicolin was included in cotreatment with CPT, indicating that replication fork activity was not involved in making TOP1 a substrate for ubiquitination. (tmu.edu.tw)
  • Specific inhibitors of eukaryotic DNA synthesis and DNA polymerase alpha, 3-deoxyaphidicolin and aphidicolin-17-monoacetate. (neb.com)
  • Treatments with a DNA synthesis inhibitor, aphidicolin, show that the number of nuclear divisions, and perhaps the DNA to cytoplasmic ratio, are critical for the appearance of lineage-specific differentiation.Conclusion: Our work corroborates previous studies demonstrating that the cleavage program is causally involved in the spatial segregation and/or activation of factors that give rise to distinct cell types in ctenophore development. (illinois.edu)
  • Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha. (neb.com)
  • Natural aphidicolin is a secondary metabolite of the fungus Nigrospora oryzae. (wikipedia.org)
  • Aphidicolin increased CREB activity and ectopic expression of dominantnegative inhibitor of CREB, A-CREB, repressed the stimulatory effects of aphidicolin on eNOS gene expression and its promoter activity. (biomolther.org)
  • Aphidicolin increased p-ATM-Ser 1981 and the knockdown of ATM using siRNA attenuated all stimulatory effects of aphidicolin on p-Akt-Ser 473 , p-CREB-Ser 133 , eNOS expression, and NO production. (biomolther.org)
  • Additionally, these stimulatory effects of aphidicolin were similarly observed in human umbilical vein endothelial cells. (biomolther.org)
  • Common fragile sites (CFSs) are found widely distributed in the population, with the largest subset of these sites being induced by aphidicolin (APH). (nih.gov)
  • Reference: DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes. (neb.com)
  • The fragile X site, which can also be induced by thymidylate stress, was not induced by aphidicolin in lymphocytes, suggesting a separate mechanism for its induction. (neb.com)
  • The hot spots induced by aphidicolin represent a new class of fragile sites which we term common fragile sites. (neb.com)
  • 5. Determination of the specificity of aphidicolin-induced breakage of the human 3p14.2 fragile site. (nih.gov)
  • Here we report that low doses of hydroxyurea, an inhibitor of ribonucleotide reductase and an important drug in the treatment of sickle cell disease and other diseases induces a high frequency of de novo CNVs in cultured human cells that resemble pathogenic and aphidicolin-induced CNVs in size and breakpoint structure. (nih.gov)
  • 1. A 350-kb cosmid contig in 3p14.2 that crosses the t(3;8) hereditary renal cell carcinoma translocation breakpoint and 17 aphidicolin-induced FRA3B breakpoints. (nih.gov)
  • Following exposure to aphidicolin, double-strand breaks were not correctly repaired in cells expressing mtRpa1. (medscape.com)
  • Aphidicolin represents a novel tool for detection of hot spots on human chromosomes through the mechanism of DNA polymerase alpha inhibition. (neb.com)
  • In contrast, aphidicolin, which blocks cell growth through inhibition of DNA polymerase alpha, has no effect on the DHFR promoter. (nih.gov)
  • Mutally exclusive inhibition of herpesvirus DNA polymerase by aphidicolin, phosphonoformate, and acyclic nucleoside triphosphate. (microbiologyresearch.org)
  • Using a genotoxic agent aphidicolin, we investigated how DDRs regulate NO production in bovine aortic endothelial cells. (biomolther.org)
  • In conclusion, our results demonstrate that in response to aphidicolin, activation of ATM/Akt/CREB/eNOS signaling cascade mediates increase of NO production and vessel relaxation in endothelial cells and rat aortas. (biomolther.org)
  • To generate mature RPE monolayers, we induced primary cilium in committed RPE cells using known cilia inducers like aphidicolin and PGE2. (nih.gov)
  • Targeting DNA repair with aphidicolin sensitizes primary chronic lymphocytic leukemia cells to purine analogs. (uclouvain.be)
  • Here we describe a protocol to identify at genome wide and at high resolution the genomic sites where MiDAS occurs in cells exposed to aphidicolin. (ox.ac.uk)
  • We demonstrate that these proteins associate with chromatin and are phosphorylated when replication is inhibited by aphidicolin. (ox.ac.uk)
  • We further demonstrate that XRad17 is essential for the chromatin binding and checkpoint-dependent phosphorylation of X9-1-1 and for the activation of XChk1 when the replication checkpoint is induced by aphidicolin. (ox.ac.uk)
  • Prolonged (over 24 h) treatment with aphidicolin increased NO production and endothelial NO synthase (eNOS) protein expression, which was accompanied by increased eNOS dimer/monomer ratio, tetrahydrobiopterin levels, and eNOS mRNA expression. (biomolther.org)
  • A promoter assay using 5'-serially deleted eNOS promoters revealed that Tax-responsive element site, located at −962 to −873 of the eNOS promoter, was responsible for aphidicolin-stimulated eNOS gene expression. (biomolther.org)
  • Co-treatment with LY294002 decreased the aphidicolin-stimulated increase in p-CREB-Ser 133 level, eNOS expression, and NO production. (biomolther.org)
  • Furthermore, ectopic expression of dominant-negative Akt construct attenuated aphidicolin-stimulated NO production. (biomolther.org)
  • Lastly, aphidicolin increased acetylcholine-induced vessel relaxation in rat aortas, which was accompanied by increased p-ATM-Ser 1981 , p-Akt-Ser 473 , p-CREB-Ser 133 , and eNOS expression. (biomolther.org)
  • At concentrations that did not greatly affect mitotic index, aphidicolin induced a striking number of chromosome gaps and breaks distributed in a highly nonrandom manner in cultured human lymphocytes. (neb.com)
  • 7. Precise localization of aphidicolin-induced breakpoints on the short arm of human chromosome 3. (nih.gov)
  • The sites most sensitive to aphidicolin damage include the 'hot spots' seen under conditions of thymidylate stress and in studies of spontaneous chromosomal damage. (neb.com)