• We analyzedthe architecture of the APC using a recently constructed budding yeaststrain that is viable in the absence of normally essential APC subunits.We found that the largest subunit, Apc1, serves as a scaffold thatassociates independently with two separable subcomplexes, one thatcontains Apc2 (Cullin), Apc11 (RING), and Doc1/Apc10, and another thatcontains the three TPR subunits (Cdc27, Cdc16, and Cdc23). (embl-heidelberg.de)
  • Apc4,Apc5, Cdc23, and Apc1 associate interdependently, such that loss of anyone subunit greatly reduces binding between the remaining three.Intriguingly, the cullin and TPR subunits both contribute to the bindingof Cdh1 to the APC. (embl-heidelberg.de)
  • Our structure explains how this TPR sub-complex, together with additional scaffolding subunits (Apc1, Apc4 and Apc5), coordinate the juxtaposition of the catalytic and substrate recognition module (Apc2, Apc11 and Apc10 (also known as Doc1)), and TPR-phosphorylation sites, relative to co-activator, regulatory proteins and substrates. (ox.ac.uk)
  • We found thatthe three TPR subunits display a sequential binding dependency, with Cdc27the most peripheral, Cdc23 the most internal, and Cdc16 between. (embl-heidelberg.de)
  • Three conserved tetratricopeptide repeat (TPR) subunits (Cdc16, Cdc23 and Cdc27) share related superhelical homo-dimeric architectures that assemble to generate a quasi-symmetrical structure. (ox.ac.uk)
  • A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. (wikipedia.org)
  • The activity of CDKs is controlled by their binding to coactivator subunits termed Cyclins, as well as by CDK inhibitory proteins termed CKIs. (intechopen.com)
  • The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. (wikipedia.org)
  • There are 1082 APC2 domains in 1081 proteins in SMART's nrdb database. (embl-heidelberg.de)
  • Taxonomic distribution of proteins containing APC2 domain. (embl-heidelberg.de)
  • The complete taxonomic breakdown of all proteins with APC2 domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing APC2 domain in the selected taxonomic class. (embl-heidelberg.de)
  • Enzymatic assays performed with APC purified fromstrains lacking each of the essential subunits revealed that onlycdc27Delta complexes retain detectable activity in the presence of Cdh1.This residual activity depends on the C-box domain of Cdh1, but not on theC-terminal IR domain, suggesting that the C-box mediates a productiveinteraction with an APC subunit other than Cdc27. (embl-heidelberg.de)
  • Human APC2 genome location and APC2 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • Anaphase-promoting complex subunit 2 is an enzyme that in humans is encoded by the ANAPC2 gene. (wikipedia.org)
  • 14. Upregulation of anaphase promoting complex (APC) 7 as a prognostic marker for esophageal squamous cell carcinoma: A hospital based study. (nih.gov)
  • A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. (wikipedia.org)
  • We now provide evidence that inversin interacts with the anaphase promoting complex protein Apc2. (nih.gov)
  • protein_coding" "Cz01g02060.t1","No alias","Chromochloris zofingiensis","Nascent polypeptide-associated complex NAC domain [Interproscan]. (ntu.edu.sg)
  • Here, we report that the HIV-1 p6 protein suppresses HIV-1-stimulated expression of IFN-I and promotes immune evasion. (bvsalud.org)
  • Biochemical studies have shown that the vertebrate APC contains eight subunits. (wikipedia.org)
  • The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. (wikipedia.org)
  • Human APC2 genome location and APC2 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • 6. Budding yeast RSI1/APC2, a novel gene necessary for initiation of anaphase, encodes an APC subunit. (nih.gov)
  • As expected of an Apc2 target, inversin possesses D-boxes and site-directed mutagenesis of the well-conserved D-box residues abrogates inversin-Apc2 interaction. (nih.gov)
  • 19. Crystal structure of the N-terminal domain of anaphase-promoting complex subunit 7. (nih.gov)
  • 1. The expression pattern of APC2 and APC7 in various cancer cell lines and AML patients. (nih.gov)
  • The high frequency of errors raises questions as to whether the surveillance mechanism known as the spindle assembly checkpoint (SAC) that controls the anaphase-promoting complex or cyclosome (APC/C) operates effectively in oocytes. (ox.ac.uk)
  • 4. Structure of an APC3-APC16 complex: insights into assembly of the anaphase-promoting complex/cyclosome. (nih.gov)