• CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. (biolegend.com)
  • Flow cytometric analysis of CD56 (NCAM-1) expression on human peripheral blood lymphocytes. (bdbiosciences.com)
  • Fluorescence histograms depicting CD56 (NCAM-1) (or Ig isotype expression) were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. (bdbiosciences.com)
  • The antibody MEM-188 reacts with an extracellular epitope on a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. (exbio.cz)
  • CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. (exbio.cz)
  • The TotalSeq™-C Human TBNK Cocktail has been designed to react with immune cells as defined and classified by the expression of the surface antigens CD19, CD3, CD16, CD4, CD11c, CD56 (NCAM), CD14, CD8, CD45. (biolegend.com)
  • Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. (rockland.com)
  • There is an unmet need to develop novel therapies for refractory/relapsed MM. In the past few years, chimeric antigen receptor (CAR)-modified T cell therapy for MM has shown promising efficacy in preclinical and clinical studies. (biomedcentral.com)
  • Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
  • Herein we used a single bicistronic lentiviral vector to develop a new combination immunotherapy that consists of human anti-carbonic anhydrase IX (CAIX)-targeted chimeric antigen receptor (CAR) T cells engineered to secrete human anti-programmed death ligand 1 (PD-L1) antibodies at the tumor site. (oncotarget.com)
  • Both culture conditions showed high NK purity with predominance of NK CD56 bright CD16 + subset post expansion. (springer.com)
  • We also found that viable NK reg cells may be isolated by sorting on CD56 + and CD16 - NK cells, and this population can suppress allogeneic CD4 + T cells, but not T reg cells or CD8+ T cells through a non-cytolytic, cell-cell contact dependent mechanism. (haematologica.org)
  • Moreover, this is the first paper to clearly establish that a CD56 bright CD3 - CD16 - perforin- NK reg population associates with a lack of cGvHD and has several unique characteristics, including the suppression of helper T-cell function in vitro. (haematologica.org)
  • Cyto-Chex BCT has been FDA 510(k) cleared for consistent recovery of HIV-associated lymphocyte subsets (CD3, CD4, CD8, CD19, CD16/CD56 and CD45) for up to 14 days. (streck.com)
  • In contrast, activity of natural killer cells, which are mostly CD16 or CD56 cells, is normal. (msdmanuals.com)
  • Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ, Phillips JH: Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). (exbio.cz)
  • CD56+ cells did not show any adipogenic activity, but a few CD56?PDGFRand and peroxisome proliferator-activated receptor-(PPARand PDGF signaling on PDGFRand PDGF signaling on AAF-CMK PDGFRphosphorylation is represented as the ratio to unstimulated control cells (cont) and shown as meanss.d. of three independent preparations. (phytid.org)
  • Occasional lymphocytes expressed CD4, CD5, CD8 and CD30, while staining for the B-cell marker CD20 and for CD56, activin receptor-like kinase 1 (Alk1) and T-cell receptor alpha/beta (TCR alpha/beta) was consistently negative. (upmc.edu)
  • Responses to mitogens and to standard vaccine antigens are determined to evaluate WBC and antibody function. (msdmanuals.com)
  • NK cells contribute to cancer immune surveillance not only by their direct natural cytotoxicity which is triggered rapidly upon stimulation through germline-encoded cell surface receptors, but also by modulating T-cell mediated antitumor immune responses through maintaining the quality of dendritic cells and enhancing the presentation of tumor antigens. (frontiersin.org)
  • Furthermore, similar to T cells, specific recognition and elimination of cancer cells by NK cells can be markedly enhanced through expression of chimeric antigen receptors (CARs), which provides an opportunity to generate NK-cell therapeutics of defined specificity for cancer immunotherapy. (frontiersin.org)
  • Innate lymphoid cells (ILCs) lack antigen receptors but are equipped with receptors for cytokines, hormones, and paracrine signals enabling them to sense their environment and respond quickly by producing factors that influence parenchymal cells and other resident immune cells. (elifesciences.org)
  • Expression of inhibitory receptors in natural killer (CD3(-)CD56(+)) cells and CD3(+)CD56(+) cells in the peripheral blood lymphocytes and tumor infiltrating lymphocytes in patients with primary hepatocellular carcinoma. (wikigenes.org)
  • Several antigens have been used as targets for CAR-T cell therapy against MM, including B cell maturation antigen (BCMA), CD19, CD138, signaling lymphocytic activation molecule 7 (SLAM7), and immunoglobulin light chains. (biomedcentral.com)
  • Cyto-Chex BCT is a direct draw blood collection tube that maintains cellular morphology and surface antigen expression, including cluster of differentiation (CD) markers prior to analysis by flow cytometry. (streck.com)
  • Recently, insulinoma-associated protein 1 (INSM1) has been identified as a novel neuroendocrine marker that is more sensitive than chromogranin, synaptophysin, and CD56 in pulmonary neuroendocrine tumors. (gastrores.org)
  • We studied this marker in conjunction with chromogranin, synaptophysin, and CD56 in 36 appendiceal AdexGCCs (21 primaries, 15 metastatic). (gastrores.org)
  • Primary and metastatic AdexGCCs showed no difference in INSM1, chromogranin, synaptophysin, or CD56 staining. (gastrores.org)
  • In addition, they were negative for the natural killer cell antigens CD56 and CD57. (nih.gov)
  • NK expansion and activation were stimulated by culture with artificial antigen-presenting cells (aAPCs) and IL-2, in the presence or absence of BM-MSCs. (springer.com)
  • The EasySep™ Human CD56 Positive Selection Kit II is designed to isolate CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) cultures by positive selection. (stemcell.com)
  • Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD56 (MEM-188) FITC. (exbio.cz)
  • This phenomenon could be offset by a dual-CAR system incorporating both an activating CAR against the cognate tumor antigen and an NK self-recognizing inhibitory CAR that transferred a 'don't kill me' signal to NK cells upon engagement with their TROG + siblings. (nature.com)
  • NK cells are a subpopulation of lymphocytes that express the CD56 + antigen on their cell surface. (articlebiz.com)
  • The CD56 antigen is present in Blastic NK cell Lymphoma, which is a known surface marker for natural killer cells. (lymphomainfo.net)
  • In large patient populations, we have shown a CD56 bright natural killer (NK) population to strongly associate with a lack of cGvHD and we hypothesize that these cells function to suppress cGvHD. (haematologica.org)
  • Transcriptome analysis of a small patient cohort of CD56 bright compared to CD56 dim NK cells found the NK reg cells to also overexpress Granzyme K, IL-7R, GPR183, RANK, GM-CSFR, TCF7, and IL23A. (haematologica.org)
  • Various immune-suppressive cell populations appear to associate with suppression of cGvHD, including T reg cells, B reg cells, M2 macrophages, dendritic cells, and CD56 bright natural killer (NK) cells. (haematologica.org)
  • Here, we discuss effects of the GB tumor microenvironment on NK-cell functionality, summarize early treatment attempts with ex vivo activated NK cells, and describe relevant CAR target antigens validated with CAR-T cells. (frontiersin.org)
  • Interestingly, the number of CD80 + pDC positively correlates with the presence of IL-10-producing regulatory type 1 cells (Tr1), an important cell type for maintaining peripheral tolerance to self-antigens. (hindawi.com)
  • DC are professional antigen-presenting cells with the unique capacity to polarize the differentiation of T cells, thereby regulating the balance between inflammation and tolerance [ 12 ]. (hindawi.com)
  • We observed 434 M-cadherin+ sublaminar satellite cells on muscle sections from 10 different patients, and found 99.5% of them were also positive for CD56 (Figure 1b). (phytid.org)
  • d) Sorted CD56+ cells were reanalyzed for PDGFRand CD56 expression. (phytid.org)
  • Immunohistochemically, the vast majority of the infiltrating cells stained positive for CD3 (Figure 6 ) and over half of them were positive for CD2 and T-cell intracellular antigen 1 (TIA1). (upmc.edu)
  • Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. (biolegend.com)
  • McCluggage WG, McKenna M, McBride HA: CD56 is a sensitive and diagnostically useful immunohistochemical marker of ovarian sex cord-stromal tumors. (exbio.cz)
  • In the current World Health Organization (WHO) classification of lymphoid malignant neoplasms, the diagnostic entity termed blastic NK-cell tumors has been proposed for tumors satisfying the diagnostic criteria for CD4/CD56 HN. (medscape.com)
  • Fig. 4: A lower level of CAR-mediated TROG-antigen expression was associated with improved clinical response to CAR-NK cell-based immunotherapy. (nature.com)
  • To perform this function, DC are capable of capturing antigens, processing them, and presenting them on the cell surface complexed to major histocompatibility (MHC) molecules, for example, human leukocyte antigen- (HLA-) DR. Along with that, context-dependent expression of costimulatory molecules, such as CD80 and CD86, and secretion of cytokines occurs. (hindawi.com)
  • Populations positive for these markers were clearly observed in varying percentages in 30 different preparations (PDGFRand CD56 expression after two passages (totally, three passages). (phytid.org)
  • The cell surface phenotype of PDGFRand CD56 expression. (phytid.org)
  • c) Sorted PDGFRand CD56 expression. (phytid.org)
  • CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours. (exbio.cz)
  • Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human CD56 standard solutions into the precoated 96-well plate. (rockland.com)
  • Blastic natural killer (NK) cell lymphoma, also termed CD4+CD56+ hematodermic neoplasm (CD4/CD56 HN) is a rare clinical entity encompassing distinct genetic, morphologic, etiologic, and diagnostic criteria. (medscape.com)
  • Fig. 2: Impact of antigen-induced self-engagement on CAR-NK effector cell phenotype and function. (nature.com)
  • Fig. 3: Impact of TROG-antigen acquisition on CAR-NK cell phenotype and function in vivo. (nature.com)
  • Human PBMCs stained with 170Er-anti-CD3 (UCHT1) and 176Yb-anti-CD56 (HCD56). (biolegend.com)
  • Whole blood was stained with either Alexa Fluor®647 Mouse Anti-Human CD56 (Cat. (bdbiosciences.com)
  • We recommend that each human CD56 standard solution and each sample is measured in duplicate. (rockland.com)
  • CAR T cell trogocytosis and cooperative killing regulate tumour antigen escape. (nature.com)
  • Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. (exbio.cz)
  • Two main advantages of BCMA as an antigen for CAR-T therapy are the potential reduction of on-target/off-tumor toxicity and the lack of antigen-dependent reduction in CAR-T cell expansion [ 16 ]. (biomedcentral.com)
  • Additionally, a comprehensive overview of antigen selection is presented along with preliminary results and future directions of CAR-T therapy development. (biomedcentral.com)
  • LewisY blood antigen or simply BG8, is a blood group antigen that has been identified in studies as a potential marker for differentiating pulmonary adenocarcinoma (PACA) from epithelioid mesothelioma (EM). (neuromics.com)