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  • Immunization
  • Therefore, surface lipophosphoglycans may play an important role in the preferential agglutination of pathogenic amebas by Con A. The protective ability of antibody EH5 was tested in a passive immunization experiment in a severe combined immunodeficient (SCID) mouse model. (nih.gov)
  • antigen
  • Frequencies and levels of IgG antibodies to CSP, LSA-1, thrombospondin-related adhesive protein (TRAP), apical membrane antigen 1 (AMA-1), erythrocyte binding antigen 175 (EBA-175), and merozoite surface protein 1 (MSP-1) were compared in 243 Kenyans living in a highland area of unstable transmission and 210 residents of a nearby lowland area of stable transmission. (umassmed.edu)
  • Protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of Entamoeba histolytica. (nih.gov)
  • Antibody EH5 strongly agglutinated amebas in a similar way to concanavalin A (Con A), and Con A bound to immunoaffinity-purified EH5 antigen. (nih.gov)
  • Localization of the antigen binding to antibody EH5 in E. histolytica trophozoites by indirect immunofluorescence using confocal microscopy. (nih.gov)
  • To determine the cellular localization of the antigen recognized by the EH5 antibody in the amebas by independent means, immunolocalization experiments on the light and electron microscopic levels were performed. (nih.gov)
  • The CSP antigen causes the production of antibodies capable of preventing the invasion of hepatocytes and additionally elicits a cellular response enabling the destruction of infected hepatocytes. (wikipedia.org)
  • infection
  • Antibodies that induce phagocytosis of malaria infected erythrocytes: effect of HIV infection and correlation with clinical outcomes. (nih.gov)
  • In women with placental malaria infection, phagocytic antibodies to CS2(VSA) were decreased in the presence of HIV (p = 0.011) and correlated positively with infant birth weight (coef = 3.57, p = 0.025), whereas total IgG to CS2(VSA) did not. (nih.gov)
  • Effect of HIV on the anti-CS2VSA antibody levels in the 'No Malaria' histopathology group.In a univariate analysis of women that showed no evidence of either peripheral or placental malaria infection, using Mann-Whitney ranksum tests, phagocytic antibodies (A) are shown to be significantly decreased by HIV infection (z = −2.17, p = 0.03). (nih.gov)
  • Mice (n = 10/group) receiving LACK or control DNA vaccination were treated with anti-CD8 antibody intraperitoneally starting at the time of vaccination (V) or at the time of infection (I) and then weekly until 4 wk after infection. (nih.gov)
  • To assess the mechanism by which LACK DNA vaccination enhanced production of IFN-γ, mice were vaccinated with LACK DNA and treated weekly from the time of initial vaccination until 4 wk after infection with a neutralizing antibody against IL-12. (nih.gov)
  • Infection is prevented by inducing high antibody titers that block the parasite from infecting the liver. (wikipedia.org)
  • parasitic
  • As a parasitic protozoan, C.luciliae lacks the ability to biosynthetically produce purine bases and therefore needs to salvage them from the surrounding environment. (wikipedia.org)
  • parasite
  • The presence of these blocking antibodies also completely abrogates the inhibitory effect of mAb 12.8 on erythrocyte invasion by the parasite in vitro. (nih.gov)
  • It is possible that Peruvians have increased antibody responses to the shared sites of PfMSP1-19KD, either due to exposure/parasite characteristics or due to a human-genetic predisposition. (nih.gov)
  • responses
  • We propose that long-lived antibody responses may not always be a prerequisite for protection, and that antibody longevity varies in an exposure- and age-dependent manner. (forskningsdatabasen.dk)
  • Here, the potential for allele-specific polymorphisms in this population is investigated, and the allele-specificity of antibody responses to PfMSP1-19KD are determined. (nih.gov)
  • To test whether PfMSP1-19KD genetic diversity had any impact on antibody responses, ELISAs testing IgG antibody response were performed on individuals using all four allele-types of PfMSP1-19KD. (nih.gov)
  • Antibody responses in 105 individuals showed that Q-KNG and Q-TSR alleles generated the strongest immune responses, while Q-KNG and E-KNG responses were more concordant with each other than with those from Q-TSR and E-TSR, and vice versa. (nih.gov)
  • Protein
  • In their assay systems, when Mab for 30 kDa protein was used as a antibody of solid phase and Mab for 24.5 kDa protein was labeled with biotin and streptolysin, the sensitivity and specificity of the reaction showed excellent results. (nii.ac.jp)
  • significantly
  • HIV significantly affected the function of phagocytic antibodies (z = −2.17, P = 0.03) (Figure 2A), but not the total IgG to CS2VSA (Z = −1.35, P = 0.179) (Figure 2B). (nih.gov)
  • Most significantly, affinity-purified, naturally acquired human antibodies specific for epitopes within the NH2-terminal 83-kD domain of MSP-1 very effectively block the processing-inhibitory activity of the anti-MSP-119 mAb 12.8. (nih.gov)
  • Microscopy
  • When fixed trophozoites were permeabilized by using Triton X-100 to solubilize the membrane lipids (method B), labeling with antibody EH5 and confocal microscopy (Fig. 5 B) revealed labeling of the periplasmic membrane as well as internal vesicle membranes. (nih.gov)
  • Animals
  • Intrahepatic challenge of animals after administration of an isotype-matched control antibody or without treatment led to the development of a liver abscess in all cases, whereas 11 out of 12 animals immunized with the EH5 antibody developed no liver abscess. (nih.gov)
  • invasion
  • Certain other anti-MSP-119 mAbs, which themselves inhibit neither erythrocyte invasion nor MSP-1 secondary processing, block the processing-inhibitory activity of the first group of antibodies and are termed blocking antibodies. (nih.gov)
  • We have now directly quantitated antibody-mediated inhibition of MSP-1 secondary processing and invasion, and the effects on this of blocking antibodies. (nih.gov)
  • Processing-inhibitory anti-MSP119 mAbs can prevent MSP-1 and erythrocyte invasion in in vitro culture, and can be rendered ineffective by the simultaneous presence of anti-pME6 blocking antibodies. (nih.gov)
  • transmission
  • Levels of antibodies to CSP, LSA-1, TRAP, and AMA-1 in the oldest age group (>40 years) in the unstable transmission area were lower than or similar to those of children 2 to 6 years old in the stable transmission area. (umassmed.edu)
  • high
  • N = 105, dark grey = high positive antibody response (values greater than 2 * negative cut-off for each allele), medium grey = medium positive response (values between 1.5 * the negative cut-off and 2 * the negative cut-off), light grey = low positive response (values between the negative cut-off and 1.5 * negative cut-off), and white = negative antibody response (values less than the negative cut-off). (nih.gov)
  • disease
  • By using C.luciliae as a substrate for immunofluorescence, the organelle can be used to detect anti-dsDNA antibodies, a common feature of the disease. (wikipedia.org)
  • species
  • Antibody EH5 gave a polydisperse band in immunoblots from membrane preparations from different E. histolytica strains, and a much weaker signal from two strains of the nonpathogenic species Entamoeba dispar. (nih.gov)
  • response
  • Previous work demonstrated a strong association between this population's antibody response to PfMSP1-19KD and protection against febrile illness and parasitaemia. (nih.gov)
  • antigens
  • The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody-based tool for detecting and quantifying antigens of interest. (biocompare.com)
  • Activated Effector T cells can be placed into three functioning classes, detecting peptide antigens originating from various types of pathogen: The first class being Cytotoxic T cells, which kill infected target cells by apoptosis without using cytokines, the second class being TH1 cells, which primarily function to activate macrophages, and the third class being TH2 cells, which primarily function to stimulate B cells into producing antibodies. (wikipedia.org)
  • It has been suggested that absorption of trichophyton fungal antigens can give rise to immunoglobulin E (IgE) antibody production, sensitization of the airways, and symptomatic asthma and rhinitis. (wikipedia.org)
  • He continued to research antibodies and antigens. (wikipedia.org)
  • ELISA
  • In 32 individuals serum samples were collected at all time points and IgG antibody reactivity detected by ELISA was variable and usually persisting not longer than one year. (scielo.br)
  • Bacterial
  • Scab may refer to: Scab, a hard coating on the skin formed during the wound healing reconstruction phase scAb, single-chain antibody fragment Apple scab, an apple tree (genus Malus) fungal disease caused by Venturia inaequalis Black scab, a potato fungal disease caused by Synchytrium endobioticum Common scab, a plant bacterial disease caused by Streptomyces species. (wikipedia.org)
  • cause
  • Babesia species are in the phylum Apicomplexa, which also has the protozoan parasites that cause malaria, toxoplasmosis, and cryptosporidiosis. (wikipedia.org)