• Immunization
  • Therefore, surface lipophosphoglycans may play an important role in the preferential agglutination of pathogenic amebas by Con A. The protective ability of antibody EH5 was tested in a passive immunization experiment in a severe combined immunodeficient (SCID) mouse model. (nih.gov)
  • infection
  • Mice (n = 10/group) receiving LACK or control DNA vaccination were treated with anti-CD8 antibody intraperitoneally starting at the time of vaccination (V) or at the time of infection (I) and then weekly until 4 wk after infection. (nih.gov)
  • To assess the mechanism by which LACK DNA vaccination enhanced production of IFN-γ, mice were vaccinated with LACK DNA and treated weekly from the time of initial vaccination until 4 wk after infection with a neutralizing antibody against IL-12. (nih.gov)
  • Infection is prevented by inducing high antibody titers that block the parasite from infecting the liver. (wikipedia.org)
  • antigen
  • Frequencies and levels of IgG antibodies to CSP, LSA-1, thrombospondin-related adhesive protein (TRAP), apical membrane antigen 1 (AMA-1), erythrocyte binding antigen 175 (EBA-175), and merozoite surface protein 1 (MSP-1) were compared in 243 Kenyans living in a highland area of unstable transmission and 210 residents of a nearby lowland area of stable transmission. (umassmed.edu)
  • Protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of Entamoeba histolytica. (nih.gov)
  • Antibody EH5 strongly agglutinated amebas in a similar way to concanavalin A (Con A), and Con A bound to immunoaffinity-purified EH5 antigen. (nih.gov)
  • Localization of the antigen binding to antibody EH5 in E. histolytica trophozoites by indirect immunofluorescence using confocal microscopy. (nih.gov)
  • To determine the cellular localization of the antigen recognized by the EH5 antibody in the amebas by independent means, immunolocalization experiments on the light and electron microscopic levels were performed. (nih.gov)
  • The CSP antigen causes the production of antibodies capable of preventing the invasion of hepatocytes and additionally elicits a cellular response enabling the destruction of infected hepatocytes. (wikipedia.org)
  • Malaria
  • In contrast, antibody levels to and frequencies of MSP-1 and EBA-175 were similar in adults in areas of stable and unstable malaria transmission. (umassmed.edu)
  • It has long been speculated that malaria parasites are directly able to undermine the establishment and maintenance of immunological memory, and that the often transient antibody responses to this parasite are evidence of such a dysfunction. (forskningsdatabasen.dk)
  • Antibodies that inhibit malaria merozoite surface protein-1 processing and erythrocyte invasion are blocked by naturally acquired human antibodies. (nih.gov)
  • and (c) have the potential to abolish protection mediated by anti-MSP-119 antibodies.Our results suggest that an effective MSP-119-based falciparum malaria vaccine should aim to induce an antibody response that prevents MSP-1 processing on the merozoite surface. (nih.gov)
  • parasitic
  • As a parasitic protozoan, C.luciliae lacks the ability to biosynthetically produce purine bases and therefore needs to salvage them from the surrounding environment. (wikipedia.org)
  • parasite
  • The presence of these blocking antibodies also completely abrogates the inhibitory effect of mAb 12.8 on erythrocyte invasion by the parasite in vitro. (nih.gov)
  • It is possible that Peruvians have increased antibody responses to the shared sites of PfMSP1-19KD, either due to exposure/parasite characteristics or due to a human-genetic predisposition. (nih.gov)
  • responses
  • We propose that long-lived antibody responses may not always be a prerequisite for protection, and that antibody longevity varies in an exposure- and age-dependent manner. (forskningsdatabasen.dk)
  • Here, the potential for allele-specific polymorphisms in this population is investigated, and the allele-specificity of antibody responses to PfMSP1-19KD are determined. (nih.gov)
  • To test whether PfMSP1-19KD genetic diversity had any impact on antibody responses, ELISAs testing IgG antibody response were performed on individuals using all four allele-types of PfMSP1-19KD. (nih.gov)
  • Antibody responses in 105 individuals showed that Q-KNG and Q-TSR alleles generated the strongest immune responses, while Q-KNG and E-KNG responses were more concordant with each other than with those from Q-TSR and E-TSR, and vice versa. (nih.gov)
  • Erythrocytes
  • There was highly significant correlated between babesial soluble protein in serum of canine … More infected with B. gibsoni and percentages of protozoa in erythrocytes (r=0.879). (nii.ac.jp)
  • Animals
  • Intrahepatic challenge of animals after administration of an isotype-matched control antibody or without treatment led to the development of a liver abscess in all cases, whereas 11 out of 12 animals immunized with the EH5 antibody developed no liver abscess. (nih.gov)
  • Protein
  • In their assay systems, when Mab for 30 kDa protein was used as a antibody of solid phase and Mab for 24.5 kDa protein was labeled with biotin and streptolysin, the sensitivity and specificity of the reaction showed excellent results. (nii.ac.jp)
  • Microscopy
  • When fixed trophozoites were permeabilized by using Triton X-100 to solubilize the membrane lipids (method B), labeling with antibody EH5 and confocal microscopy (Fig. 5 B) revealed labeling of the periplasmic membrane as well as internal vesicle membranes. (nih.gov)
  • significantly
  • Most significantly, affinity-purified, naturally acquired human antibodies specific for epitopes within the NH2-terminal 83-kD domain of MSP-1 very effectively block the processing-inhibitory activity of the anti-MSP-119 mAb 12.8. (nih.gov)
  • invasion
  • Certain other anti-MSP-119 mAbs, which themselves inhibit neither erythrocyte invasion nor MSP-1 secondary processing, block the processing-inhibitory activity of the first group of antibodies and are termed blocking antibodies. (nih.gov)
  • We have now directly quantitated antibody-mediated inhibition of MSP-1 secondary processing and invasion, and the effects on this of blocking antibodies. (nih.gov)
  • Processing-inhibitory anti-MSP119 mAbs can prevent MSP-1 and erythrocyte invasion in in vitro culture, and can be rendered ineffective by the simultaneous presence of anti-pME6 blocking antibodies. (nih.gov)
  • transmission
  • Levels of antibodies to CSP, LSA-1, TRAP, and AMA-1 in the oldest age group (>40 years) in the unstable transmission area were lower than or similar to those of children 2 to 6 years old in the stable transmission area. (umassmed.edu)
  • high
  • N = 105, dark grey = high positive antibody response (values greater than 2 * negative cut-off for each allele), medium grey = medium positive response (values between 1.5 * the negative cut-off and 2 * the negative cut-off), light grey = low positive response (values between the negative cut-off and 1.5 * negative cut-off), and white = negative antibody response (values less than the negative cut-off). (nih.gov)
  • disease
  • By using C.luciliae as a substrate for immunofluorescence, the organelle can be used to detect anti-dsDNA antibodies, a common feature of the disease. (wikipedia.org)
  • species
  • Antibody EH5 gave a polydisperse band in immunoblots from membrane preparations from different E. histolytica strains, and a much weaker signal from two strains of the nonpathogenic species Entamoeba dispar. (nih.gov)
  • response
  • Previous work demonstrated a strong association between this population's antibody response to PfMSP1-19KD and protection against febrile illness and parasitaemia. (nih.gov)