• To facilitate antigenic characterization of the A(H1N1)pdm09 HA, we generated a panel of murine monoclonal antibodies (mAbs) to the HA of A/California/04/2009. (scapca.org)
  • To determine whether this was a more general phenomenon, a panel of murine monoclonals were tested and found to bind to beta-galactosidase. (cornell.edu)
  • Recombinant human/mouse chimeric monoclonal antibody that specifically binds to the extracellular domain of human epidermal growth factor receptors (EGFR, HER1, c-ErbB-1). (medscape.com)
  • Recombinant human IgG2 kappa monoclonal antibody that binds to human epidermal growth factor receptor (EGFR). (medscape.com)
  • Methods: Adult C57BL/6J mice (n=10) were intrathecally injected with a recombinant human NR1 antibody over 14 days and then studied using rs-fMRI at 7 Tesla. (sciencegate.app)
  • In addition, a newly established mouse model with in utero exposure to a human recombinant NR1 antibody characterized by a neurodevelopmental disorder (NR1-offspring) was investigated with rs-fMRI at the age of 8 weeks (n=15) and 10 months (n=14). (sciencegate.app)
  • Therefore, bioengineered and recombinant antibodies were prepared for successful administration in humans. (conductscience.com)
  • The rest of the cells had been cultured in full moderate comprising RPMI 1640 including 5% fetal leg serum, 50 M 2-mercaptoethanol, and 2 mM glutamine supplemented with 2.5 ng of recombinant murine granulocyte/macrophage colony-stimulating factor/ml and 5 ng of recombinant murine IL-4/ml. (buyresearchchemicalss.net)
  • The antitumor efficacy was studied in mouse mammary carcinoma models (Her2/neu transgenic and 4T1-luc mammary cancers) treated with systemic recombinant protein with/without the depletion of myeloid-derived suppressor cells or intra-tumoral gene electrotransfer (GET). (frontiersin.org)
  • Mucosal and systemic neutralizing antibodies to norovirus induced in infant mice orally inoculated with recombinant rotaviruses. (cdc.gov)
  • Immunoglobulin M (IgM) antibodies usually develop 7-14 days after immunization with the antigen. (medscape.com)
  • When the antigen and antibody molecules are present in approximately equal molar ratios (slight antigen excess), known as the zone of equivalence, cross-linking and lattice formation occur forming intermediate and large immune complexes. (medscape.com)
  • Since the development of serum sickness is dependent on the host's ability to produce antibodies to the inciting antigen, patients who are incapable of producing antibodies, such as patients with Bruton agammaglobulinemia, will not develop serum sickness. (medscape.com)
  • [ 14 ] However, ATG, as well as other immunosuppressive foreign proteins, such as chimeric monoclonal antibodies that consist of murine-derived antigen-binding fragment (Fab) and human-derived crystallizable fragment (Fc) portions of antibodies, have been reported to be sufficiently immunogenic to cause serum sickness. (medscape.com)
  • The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. (justia.com)
  • Guided by these determinations, the antibody is accordingly modified (altered) to improve upon, e.g., antibody/antigen binding by modifying at least one amino acid residue to decrease the binding free energy between the antibody and antigen when bound in a solvent. (justia.com)
  • Researchers hoped that such antibodies would be no more foreign to the human body than a genuine human antibody, but the utility of such antibodies has been restricted, at least in some cases, by a reduction in the antibody's affinity for the antigen. (justia.com)
  • Mib-1 monoclonal antibody recognizes the proliferation-related antigen Ki-67. (lu.se)
  • Stable human antigen-specific monoclonal B cell lines were established without prior in vivo immunization. (cornell.edu)
  • It is therefore possible that human and murine anti-TNP beta cell responses reflect reactivity against an environmental antigen, namely an epitope present on E. coli-derived beta-galactosidase. (cornell.edu)
  • This approach of expanding human antigen-specific B cells by antigen stimulation in vitro, with a T-independent hapten-carrier conjugate before Epstein-Barr virus transformation, may prove useful in the development of human monoclonals for therapeutic purposes. (cornell.edu)
  • We used T cells that were retrovirally transduced with this CAR to treat mice bearing a syngeneic lymphoma that naturally expressed the self-antigen murine CD19. (ashpublications.org)
  • Anti-human globulin reagents detect non-agglutinating antibody molecules as well as molecules of complement attached to red cells following in vivo or in vitro antigen-antibody reactions. (accumed.ie)
  • Human derived T lymphocytes engineered to express chimeric antigen receptors, which are expanded in vitro culture and then infused into patients exerting robust cytotoxicity after tumor antigen recognition and subsequent activation. (biomedcentral.com)
  • ScFv is the antigen-binding domain of CAR structure, which is composed of a single heavy and light chain of monoclonal antibody connected by a linker. (biomedcentral.com)
  • Researchers have modified antibodies in various ways in order to study their function or to improve their utility as therapeutic agents. (justia.com)
  • Therapeutic Alemtuzumab antibody from the original Lemtrada® commercial drug. (evidentic.com)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
  • Antibodies obtained from a single clone of cells grown in mice or rats. (bvsalud.org)
  • In this study, we investigated adverse effects in mice receiving repeated anti-PD1 (clone J43) or PD-L1 (clone 10F.9G2) monoclonal antibody (mAb) administration in the 4T1 mouse model of mammary carcinoma. (biomedcentral.com)
  • Lorne Polyspecific Anti-Human Globulin Elite Clear and Anti-Human Globulin Elite Green reagents contain anti-IgG derived from rabbits with non-specific activity removed by absorption and murine monoclonal IgM anti-C3d, Clone BRIC-8. (accumed.ie)
  • We describe the initial characterization of these mAbs, the identification of several antibodies with neutralizing and hemagglutination activity, and the evaluation of antibodies for their protective TFRC effect in mice when administered. (scapca.org)
  • Further reduction of murine content led to the formation of 'humanized mAbs. (conductscience.com)
  • Quickly, murine bone tissue marrow cell suspensions had been incubated with an assortment of M5/114 (anti-major histocompatibility complicated [MHC] course II), RA3-6B2 (anti-B220), 53-6.7 (anti-CD8), GK1.5 (anti-CD4), and RB6-8C5 (anti-Ly-6G) monoclonal antibodies (MAbs), and stained cells were eliminated by negative selection using Dynabeads M-450 coated with sheep anti-rat immunoglobulin G (IgG). (buyresearchchemicalss.net)
  • Previously, 4T1 has been associated with high expression of granulocyte-colony stimulating factor (G-CSF) systemically resulting in increased extramedullary hematopoiesis and the accumulation of myeloid derived suppressor cells (MDSCs). (biomedcentral.com)
  • Furthermore, we describe a subset of myeloid cells with immunosuppressive activity (known as myeloid-derived suppressor cells). (hindawi.com)
  • The depletion of myeloid-derived suppressor cells (MDSCs) has no impact on mouse breast cancer growth. (frontiersin.org)
  • Adult tissue-derived mesenchymal stromal cells (MSCs) are showing promise in clinical trials for systemic lupus erythematosus (SLE). (nature.com)
  • Human embryonic stem cell (hESC)-derived MSCs are an alternative to adult MSCs that can circumvent issues regarding scalability and consistent quality due to their derivation from a renewable starting material. (nature.com)
  • Results: Adult NR1-antibody injected mice showed significantly impaired functional connectivity within the dentate gyrus of the left hippocampus in comparison to controls, resembling impaired hippocampal functional connectivity patterns observed in human patients with NMDAR encephalitis. (sciencegate.app)
  • Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). (thermofisher.com)
  • The use of monoclonal antibodies targeting these immune checkpoints has been shown to promote anti-tumor immune responses clinically. (biomedcentral.com)
  • In murine models of melanoma and pancreatic cancer, LYT-200 significantly reduced tumor growth, extended survival and modulated the intra-tumoral immune microenvironment. (bmj.com)
  • Accordingly, there remains a need for antibodies that do not provoke a strong immune response but yet bind strongly to their antigens and methods for identifying such antibodies. (justia.com)
  • Chronic Granulomatous Disease , autoimmune diseases Autoimmune diseases Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides. (lecturio.com)
  • In addition, only PrP res from H-type isolates were labeled by monoclonal antibody P4 with defined PrP res N terminus epitope specificity, in contrast with PrP res from BSE isolates, which suggests a different cleavage by proteinase K of the disease-associated protein ( 9 ). (cdc.gov)
  • NEOD001, a humanized form of murine monoclonal antibody 2A4, binds to an epitope unique to the misfolded light chain protein. (holyexperiment.org)
  • The cryptic light chain epitope is thought to be exposed during misfolding and aggregation but is not available in light chains native conformation or in fully formed immunoglobulin.9 Accordingly, 2A4 was shown to immunoreact with BMS303141 both soluble and insoluble light chain aggregates derived from samples from patients with AL while sparing normally folded light chain. (holyexperiment.org)
  • Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. (thermofisher.com)
  • Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. (thermofisher.com)
  • The following product was used in this experiment: CD279 (PD-1) Monoclonal Antibody (J43), Biotin, eBioscience™ from Thermo Fisher Scientific, catalog # 13-9985-82, RRID AB_467023. (thermofisher.com)
  • The following product was used in this experiment: CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eFluor™ 450, eBioscience™ from Thermo Fisher Scientific, catalog # 48-1051-82, RRID AB_10548959. (thermofisher.com)
  • Researchers have often considered the most likely source to be a recycled infectious agent derived from prion-associated diseases found in other species, such as scrapie in sheep and goats. (cdc.gov)
  • In addition to surgery, chemotherapy and radiation, many neuroblastoma patients at MSKCC were treated with a murine (mouse-derived) monoclonal antibody called 3F8. (wikipedia.org)
  • Atezolizumab, an anti-PD-L1 antibody is the first checkpoint inhibitor approved by FDA in combination with chemotherapy to treat triple-negative and PD-L1 positive metastatic breast cancer. (frontiersin.org)
  • Mice exposed to isotype-matched control antibodies served as controls. (sciencegate.app)
  • Independent component analysis (ICA) and dual regression analysis were performed to compare functional connectivity between NMDAR antibody mouse models and control mice. (sciencegate.app)
  • The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. (biolegend.com)
  • Chimeric antibodies prepared as a result comprised V L and V H from mice and C H1 , C H2 , and C H3 from the human. (conductscience.com)
  • A major part of humanized antibodies is human-derived, with only one CDR derived from mice. (conductscience.com)
  • Eventually, entirely human custom antibodies were prepared by employing either two technologies, i.e., phage display or transgenic mice (Ruker and Knopp. (conductscience.com)
  • LYT-200 is a fully human IgG4 monoclonal antibody targeting gal-9. (bmj.com)
  • The IgG class of antibodies is further divided into four subclasses IgG1, IgG2, IgG3, and IgG4. (conductscience.com)
  • Our results demonstrated impressive antilymphoma activity and profound destruction of normal B cells caused by anti-CD19-CAR-transduced T cells in a clinically relevant murine model. (ashpublications.org)
  • Most of the antibodies clinically used as therapeutics are immunoglobulin G (IgG) monoclonal antibodies with a similar structure comprising four polypeptide chains as mentioned above and have a molecular weight of about 150kDa. (conductscience.com)
  • LYT-200 treated patient derived tumor organoids showed an increase in T cell activation (Chen L. et al, SITC 2019-P765). (bmj.com)
  • The disease is generally categorized as warm or cold, depending on the thermal reactivity of the autoantibodies Autoantibodies Antibodies that react with self-antigens (autoantigens) of the organism that produced them. (lecturio.com)
  • When tested against a panel of self and environmental antigens, all five anti-TNP clones exhibited cross-reactivity with an Escherichia coli-derived beta-galactosidase. (cornell.edu)
  • In murine models, intracranial rechallenged tumors after initial rejection by PD-1 blockade were suppressed. (bvsalud.org)
  • Myeloid cells are a heterogeneous population of bone marrow-derived cells that play a critical role during growth and metastasis of malignant tumors. (hindawi.com)
  • p53, mdm-2, p21, and mib-1 expression were assessed by immunohistochemical methods in primary tumors derived from 134 patients who took part in a randomized multicenter trial comparing docetaxel to sequential methotrexate and 5-fluorouracil (MF) in advanced breast cancer. (lu.se)
  • IL-15cx treatment diminishes MDSCs in murine tumors. (frontiersin.org)
  • Using human post\vaccination sera, however, there were no differences in HI titer between A/California/04/2009 and either escape mutant, suggesting that these single mutations were not sufficient to abrogate a protective antibody response to the vaccine. (scapca.org)
  • Shortly after the administration of the foreign protein, the host mounts a specific antibody response to clear the foreign substance. (medscape.com)
  • The protein product of the murine double minute gene 2 (mdm-2) plays a central role in the regulation of p53. (lu.se)
  • Antibodies are large heterodimeric protein molecules consisting of four polypeptide chains, i.e., two heavy chains of 50kDa each and two light chains of 25kDa. (conductscience.com)
  • Dr Roth explained to heart wire that antidrug antibodies are those generated against any part of the foreign protein, which don't necessarily interfere with the binding to its target. (medscape.com)
  • To deliver on that promise, our laboratory is collaborating with the laboratories of Markus Grompe, M.D., OHSU, and Gordon Keller, Ph.D., University Health Network, Toronto, Canada, in the development and characterization of monoclonal antibodies directed against cell surface molecules expressed by pancreatic cell subsets as well as pancreatic progenitor cells derived from pluripotent stem cells. (ohsu.edu)
  • With the technical advancements and an understanding of their molecular properties, custom antibodies have become the most important class of molecules in immunologic research. (conductscience.com)
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)
  • Malignant epithelial cancers, the major cause of cancer morbidity and mortality, arise in organs composed of both epithelial and mesenchyme-derived stromal cells, such as fibroblasts, myofibroblasts, endothelial cells, pericytes, smooth muscle, and hematopoietic cells. (aacrjournals.org)
  • Antibody therapy targeting misfolded proteins is BMS303141 a potential new therapy for the management of AL amyloidosis. (holyexperiment.org)
  • Invasion and metastasis of malignant epithelial cells into normal tissues is accompanied by adaptive changes in the mesenchyme-derived supporting stroma of the target organs. (aacrjournals.org)
  • We discovered that a conjugated metabolite derived from quercetin possesses considerable anti-oxidant activity in several in vitro experiments. (rsc.org)
  • In vitro generated human monoclonal trinitrophenyl-specific B cell lines. (cornell.edu)
  • IL-7 was initially described as a stromal derived factor which is capable of inducing the growth of pre-B cells in vitro . (biolegend.com)
  • Application of patient-derived monoclonal antibodies against the NR1 subunit of the NMDAR allows for the investigation of potential functional connectivity alterations in experimental murine NMDAR antibody disease models. (sciencegate.app)
  • They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)
  • The effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), structural factors, experience, and availability of IgG determine the preference of one type of immunoglobulin over another (Ryman and Meibohm, 2017). (conductscience.com)
  • Results of the clinical-trial program with the proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitor bococizumab (Pfizer), including final data from the major outcome trials SPIRE 1 and 2 , show that antidrug antibodies developed in a large proportion of patients and significantly attenuated the LDL -lowering effect [ 1 , 2 ] . (medscape.com)
  • Data on antibody formation and LDL-cholesterol-lowering effects from six trials of bococizumab in a total of 4300 patients, as well as final outcome results from the SPIRE 1 and 2 trials, were presented today at the American College of Cardiology (ACC) 2017 Scientific Sessions and simultaneously published online in the New England Journal of Medicine . (medscape.com)
  • In most cases, there appears to be some level of 2009 H1N1 pandemic cross\reactive antibody in persons older than 55C60?years of age, corresponding with exposure to H1N1 viruses before the 1957 pandemic. (scapca.org)
  • In 1957, Dacie et al showed that the antibodies present in antiglobulin sera were directed against certain components of complement. (accumed.ie)
  • Because the antibody also binds to peripheral nerve cells, the treatment is painful, but it is generally without long-term complications. (wikipedia.org)
  • A group of malignant lymphomas thought to derive from peripheral T-lymphocytes in lymph nodes and other nonlymphoid sites. (lookformedical.com)
  • These cells were therefore described as bone marrow derived endothelial progenitor cells (EPCs). (hindawi.com)
  • Considerable time and effort has been spent in developing targeted therapies to fight SLE, yet only one therapy, belimumab (Benlysta), a monoclonal antibody targeting B cell-activating factor, or BAFF, has been approved for the treatment of SLE in the last half-century 16 . (nature.com)
  • However, the supply of donor-derived islets will never meet the needs of the large number of patients who could potentially benefit from this cell-based therapy. (ohsu.edu)
  • To evaluate anti-CD19-CAR-transduced T cells in a murine model of adoptive T-cell therapy, we developed a CAR that specifically recognized murine CD19. (ashpublications.org)
  • To establish a murine model in which a completely syngeneic lymphoma could be treated by adoptive transfer of syngeneic CAR-transduced T cells, we developed a CAR that could specifically recognize murine CD19. (ashpublications.org)
  • Applications Tested: The J43 antibody has been tested by flow cytometric analysis of Con A-simulated mouse splenocytes and mouse PD-1 transfected cells. (thermofisher.com)
  • Applications Tested: This MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells. (thermofisher.com)
  • Our laboratory, in collaboration with the laboratory of Jeffrey Whitsett, M.D., at the Cincinnati Children's Hospital, is generating and characterizing a panel of monoclonal antibodies targeting cell-surface markers on different subsets of pulmonary cells involved in the process of pulmonary tissue regeneration. (ohsu.edu)
  • To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)
  • Anti-CD19-CAR-transduced T cells had superior antilymphoma efficacy compared with the anti-CD19 monoclonal antibody from which the anti-CD19 CAR was derived. (ashpublications.org)
  • However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. (biolegend.com)
  • The goal of this study was to examine the activity of IL-15/IL-15Rα complex (IL-15cx) to CD8 + T cells and evaluate its potential efficacy in murine breast cancer models. (frontiersin.org)
  • But neutralizing antibodies bind to the drug at the point at which it interacts with its target and so have a higher likelihood of prevent its action. (medscape.com)
  • Lead investigator of the SPIRE 1 and 2 studies and lead author of both papers on bococizumab, Dr Paul Ridker (Harvard Medical School, Boston, MA) commented to heart wire from Medscape: "Despite antidrug antibody development, bococizumab nonetheless significantly reduced vascular events by 21% among those with higher risk and higher LDL levels. (medscape.com)
  • The earlier experiments on antibodies were based on the injection of serum of immunized animals into the diseased subjects. (conductscience.com)
  • Bone tissue marrow-derived DC had been generated by an adjustment of a way previously referred to (24). (buyresearchchemicalss.net)
  • Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2. (thermofisher.com)
  • Objective: To explore functional connectivity changes in NR1 antibody mouse models using resting-state functional MRI (rs-fMRI). (sciencegate.app)
  • PrP res extracted from half of whole brain was detected with monoclonal antibodies Sha31 (1:10 from TeSeE sheep/goat Western blot, Bio-Rad, Hercules, CA, USA) ( 14 ) and 12B2 (340 ng/mL) ( 15 ). (cdc.gov)
  • For the past few decades, custom antibodies have been used to fight against autoimmune, cancerous, and infectious diseases. (conductscience.com)
  • However, the use of equine and murine antisera as antilymphocyte or antithymocyte globulins and murine monoclonal antibodies for immunomodulation and cancer treatment has created a new group of medications that may cause serum sickness. (medscape.com)
  • In 1945, Coombs, Mourant and Race described the use of anti-human globulin serum for detecting red cell-bound non-agglutinating antibodies. (accumed.ie)
  • The nodules resemble to some extent the GERMINAL CENTER of lymph node follicles and most likely represent neoplastic proliferation of lymph node-derived follicular center B-LYMPHOCYTES. (lookformedical.com)
  • Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)