• novel
  • To further characterize the function of DJ-1, we cloned cDNA encoding a novel DJ-1-binding protein, DJBP, by a yeast two-hybrid system. (aacrjournals.org)
  • The highest expression levels together with strong repression in pre-culture were achieved with the novel promoters PG1 and PG6.Human serum albumin (HSA) was used to characterize the promoters with an industrially relevant secreted protein. (biomedsearch.com)
  • CONCLUSIONS: A set of novel regulated promoters, enabling induction without methanol, was successfully identified by using DNA microarrays and shown to be suitable for high level expression of recombinant proteins in glucose-based protein production processes. (biomedsearch.com)
  • To address the function of DJ-1 in prostate, we performed a yeast two-hybrid screen to identify novel DJ-1 binding proteins. (vanderbilt.edu)
  • These data provide a novel mechanism for DJ-1 mediated regulation of AR in the progression of prostate cancer to androgen-independence. (vanderbilt.edu)
  • Recent results suggest that novel CBP-mediated post-translational N-glycosylation activity alters the conformation of CBP-interacting proteins, leading to regulation of gene expression, cell growth and differentiation, Homeodomain interacting protein kinase 2 (HIPK2) phosphorylates several regions of CBP close to the N-terminal and close to the C-terminal region as well. (wikipedia.org)
  • Sertoli
  • We have shown that PIASxα inhibits the transcription activity of the AR by binding to the DNA-binding domain of AR and that DJ-1 antagonizes this inhibition by sequestering PIASXα from the AR in CV-1, Cos1, and TM4 Sertoli cells, indicating that DJ-1 is a positive regulator of the AR ( 3 ). (aacrjournals.org)
  • Sertoli cells bind to spermatogonial cells via N-cadherins and galactosyltransferase (via carbohydrate residues). (wikipedia.org)
  • This repair likely employs the process of non-homologous end joining involving XRCC1 and PARP1 proteins that are expressed in Sertoli cells. (wikipedia.org)
  • Immunohistochemical
  • Immunohistochemical detection of proteins on electroblots of polyacrylamide electrophoresis and isoelectric focusing gels, however shows that the prostate CRM is a protein with electrophoretic and isoelectric focusing behavior distinct from that of salivary ABP isoproteins. (springer.com)
  • interact
  • The two tables below display ChEMBL single-protein targets which are predicted to interact with CHEMBL1473. (ebi.ac.uk)
  • regulation
  • The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. (bio-rad.com)
  • The protein product of this gene, FMRP (fragile X mental retardation protein), is thought to be involved in the translational regulation of mRNAs important for learning and memory. (biochemj.org)
  • Up-regulation or activation of transcription results in increased synthesis of messenger RNA, which, in turn, is translated by ribosomes to produce specific proteins. (wikipedia.org)
  • nucleus
  • In vivo studies revealed that RELA is also under acetylation modification in the nucleus, which is just as important as phosphorylation as a post-translational modification of proteins. (wikipedia.org)
  • inhibitor
  • A small molecule inhibitor (I-CBP112) binding to the bromodomain domain of CBP/p300 has been developed for leukaemia therapy. (wikipedia.org)
  • Identification
  • p>This subsection of the 'Names and Taxonomy' section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein. (uniprot.org)
  • Mechanism
  • Correction to Probing the Binding Mechanism of Mnk Inhibitors by Docking and Molecular Dynamics Simulations. (nih.gov)
  • Human
  • With regard to the function of DJ-1 in sperm, a rat homologue of human DJ-1 has been identified by other laboratories as a key protein related to infertility of male rats that had been exposed to sperm toxicants such as ornidazole and epichlorohydrin ( 23 -26 ). (aacrjournals.org)
  • Alignment of the human, mouse, rat, rabbit and dog promoters reveals several highly conserved transcription factor-binding sites. (biochemj.org)
  • mRNA
  • Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene. (wikipedia.org)
  • The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. (wikipedia.org)
  • expression
  • The reason for reduced or absent CRM in the prostates of DBA/2J males is unknown but this finding suggests that there are strain-related differences in the expression of this prostate protein. (springer.com)
  • Therefore, PCBP1 plays a major role in the dynamic expression of AR in both male and female androgen-responsive cells. (warwick.ac.uk)
  • DJ-1 expression increased following flutamide treatment as a result of DJ-1 protein stabilization. (vanderbilt.edu)
  • DJ-1 expression does not change with Gleason pattern, but increases after androgen deprivation therapy, indicating that it may be involved in the development of androgen independence. (vanderbilt.edu)
  • cells
  • Furthermore, a co-immunoprecipitation assay showed that the formation of a ternary complex between DJ-1, DJBP, and AR occurred in cells in which DJ-1 bound to the AR via DJBP. (aacrjournals.org)
  • DJ-1 was identified as a protein that transforms mouse NIH3T3 cells in cooperation with activated ras ( 1 ). (aacrjournals.org)
  • Furthermore, knockdown of PCBP1 in decidualizing cells was sufficient to restore AR protein levels, indicating that loss of the AR protein is primarily the consequence of a translational block. (warwick.ac.uk)
  • phosphorylation
  • The fact that RELA can be modified by a collection of kinases via phosphorylation at different sites/regions within the protein under different stimulations might suggest a synergistic effect of these modifications. (wikipedia.org)
  • glands
  • Using an antibody raised against the purified protein, we demonstrated the presence of cross-reactive material (CRM) in mouse parotid, submaxillary, sublingual, and prostate glands by double immunodiffusion. (springer.com)