• Here, we further attenuated WNV by mutating the asparagine to serine or glutamine in addition to mutating other residues in the NS1(130-132) glycosylation motif. (nih.gov)
  • While most substitutions of conserved residues within these motifs severely reduced the enzymatic activities, a single substitution in motif D which enhanced the RdRp activity by about 50% was found. (nih.gov)
  • While linking amino acid residues 268-276 to a truncated EPO-R (Δ+9 EPO-R) conferred both ligand uptake and ligand-independent down regulation of the respective receptor from the cell surface, Phe 272 was crucial for EPO endocytosis but not for ligand-independent down regulation. (elsevierpure.com)
  • Additional receptor motifs probably play a role in EPO endocytosis and receptor down-regulation, as these processes were not adversely impaired in Δ268-276 EPO-R. A central role of residues 268-276, in particular Phe, was demonstrated by the inability of Δ268-276 and F268,272A EPO-Rs to support EPO-mediated signal transduction. (elsevierpure.com)
  • Mutations in motif 2 loop residues affect tRNA-dependent amino acid recognition. (nih.gov)
  • The first 20 amino acids of the beta-subunit cytoplasmic domain are also alpha helical, but the final 25 residues are disordered and, apart from a turn that follows a conserved NPxY motif, appear to lack defined structure, suggesting that this is adopted on effector binding. (embl-heidelberg.de)
  • Deduced amino acid sequence of BmSQS showed highly conserved consensus aspartate-rich motifs (DXXXD) and catalytic site residues. (springer.com)
  • By using a mutational analysis, four amino acid sequence motifs crucial for RdRp activity were identified. (nih.gov)
  • The viral protein contained seven short sequence motifs with similar placement within the polypeptide as the streptococcal and the vertebrate HASs. (gr.jp)
  • All highly conserved structural motifs and the 3D model of the DNA-binding domain indicate an unimpaired DNA-binding ability of Par CBF1. (frontiersin.org)
  • The 12-amino acid long motif contains a highly conserved lysine residue in the first position, followed by mostly charged amino acids in the second position. (eu.org)
  • The highly conserved alanine residue is found in all RPA interacting motifs. (eu.org)
  • Quantitative prediction of binding affinity revealed that HLA-DRβ1 alleles with the YST motif could be distinguished on the basis of discernable peptide-binding preferences. (nih.gov)
  • Asymmetric synthesis of substituted hydroxy amino acid motifs (peptide isosteres). (mun.ca)
  • However, the only biomolecules that have been explored for this OCS role are amino acids and amino-acid-derived peptide and protein motifs. (rsc.org)
  • Most integrins recognise relatively short peptide motifs, and in general require an acidic amino acid to be present. (embl-heidelberg.de)
  • Phylogenetic analysis using full-length amino acid sequences of functionally characterized BAHD enzymes by D'Auria (2006) and Stewart et al. (wikipedia.org)
  • For example, Clade I members act mainly upon flavonoids, while Clade V members utilize substrates ranging from terpenoids to medium-chain alcohols to quinic acid, in association with major phylogenetic branches within this clade [ 2 ]. (biomedcentral.com)
  • However this RNP-1 motif in RNA binding proteins was part of a much larger motif of about 80 amino acids. (bio.net)
  • What this found was that although the fold was entirely different in the two proteins, the two RNP-1 motifs were placed each in a beta strand with similar enviroments and in each case it was these RNP-1 motifs that it was thought were doing the binding to nucleic acid. (bio.net)
  • The derived amino acid sequence of the Wilson's disease gene (GenBank, NM 000053) reveals the presence of motifs conserved in all members of the P-type family of cation transport proteins. (medscape.com)
  • As with other mitochondrial carrier family proteins, the ornithine carrier is composed of 300 amino acids that constitute 3 repeated motifs of approximately 100 amino acids each. (medscape.com)
  • Previous research has shown that characterized proteins from this family fall broadly into five major clades and contain two conserved protein motifs. (biomedcentral.com)
  • These motifs allow molecules of melanocyte inducing transcription factor to interact with each other or with other proteins that have a similar structure, creating a two-protein unit (dimer) that functions as a transcription factor. (medlineplus.gov)
  • TDP43 contains two copies of a ~90 amino acid RNA recognition motif (RRM) found in many proteins which bind single stranded RNA. (biolegend.com)
  • The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. (genscript.com)
  • We detected amino acid motifs predicted to be B cell epitopes in Zika virus proteins which are also present in human proteins, including pro-neuropeptide Y (proNPY), NAV2 and other proteins with interacting neurophysiologic function. (biorxiv.org)
  • The RPA interacting motif is conserved in eukaryotes DNA repair and replication proteins UNG2, XPA, RAD14, TIPIN and SMARCAL1. (eu.org)
  • We hypothesized that phosphorylation of the C-terminal T/SXV motif of NR2B may regulate the interactions between NMDARs and PSD-95/SAP90 family proteins. (jneurosci.org)
  • Deduced amino acid sequences of urotensin II receptor (UTR) subtypes identified in Xenopus tropicalis. (xenbase.org)
  • Integrin cytoplasmic domains are normally less than 50 amino acids in length, with the beta-subunit sequences exhibiting greater homology to each other than the alpha-subunit sequences. (embl-heidelberg.de)
  • A cadherin repeat is an independently folding sequence of approximately 110 amino acids that contains motifs with the conserved sequences DRE, DXNDNAPXF, and DXD. (embl.de)
  • The Histidine in this motif has also been shown to act as a catalytic residue in many BAHD enzymes. (wikipedia.org)
  • Bushman, F. D., Shang, C. & Ptashne, M. One glutamic acid residue plays a key role in the activation function of lambda represser. (nature.com)
  • By generating E2F4/E2F1 chimeras, we identify a six-residue motif that is critical for Deup1 and SAS6 binding. (molbiolcell.org)
  • We found that casein kinase II (CK2) directly phosphorylates the -2 serine residue (Ser1480) within the C-terminal T/SXV motif of NR2B. (jneurosci.org)
  • MyoD family members share about 80% amino acid homology in their bHLH motifs. (novusbio.com)
  • These sites exhibited distinct dynamic phosphorylation patterns, which were linked to known or predicted kinases on the basis of the matching sequence motif. (nih.gov)
  • The determination of consensus phosphorylation site motifs by amino acid sequence alignment of known substrates has proven useful in this pursuit. (neb.com)
  • These motifs can be helpful for predicting phosphorylation sites for specific protein kinases within a potential protein substrate. (neb.com)
  • Expression of phosphorylation-defective PLIN5 S155A in Plin5 null cells resulted in decreased rates of lipolysis and triglyceride-derived fatty acid oxidation. (uci.edu)
  • We identified two new multi-clade motifs and numerous clade-specific motifs, several of which have been implicated in BAHD function by previous structural and mutagenesis research. (biomedcentral.com)
  • Kuldell, N. & Hochschild, N. Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. (nature.com)
  • In contrast to the widely held belief that one enzyme transfers one sugar, the HASs co-polymerize both N -acetylglucosamine [GlcNAc] and glucuronic acid [GlcUA] into the hyaluronan chain. (gr.jp)
  • The WHO Collaborating Center for Influenza Reference and Research at the Centers for Disease Control and Prevention in Atlanta, with input from many influenza subject matter experts from around the world, has developed an inventory of amino acid mutations in H5N1 viruses that is intended to be used by the influenza surveillance and research community as a tool to inform the influenza knowledge base for surveillance and public health preparedness. (cdc.gov)
  • Likewise, an investigation of common disease variants localized to the Walker-A motif of the nucleotide-binding-domains (NBDs) could help understand how ABCA4 couples binding and hydrolysis of ATP with N-ret-PE transport. (ubc.ca)
  • The MITF gene variants that cause Tietz syndrome either delete or change a single protein building block (amino acid) in the basic motif region of the melanocyte inducing transcription factor structure. (medlineplus.gov)
  • Some MITF gene variants change the amino acids used to make melanocyte inducing transcription factor, which alters the helix-loop-helix or leucine-zipper motif. (medlineplus.gov)
  • A full-length SQS gene was isolated from B. monniera , characterized as B. monniera squalene synthase ( BmSQS ) (1242 bp) encoding 414 amino acids. (springer.com)
  • The other region, known as the basic motif, binds to specific areas of DNA, allowing the dimer to control gene activity. (medlineplus.gov)
  • This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. (genscript.com)
  • The H5N1 Inventory is an inclusive compilation of amino acid changes and/or motifs identified within each viral protein that affect one or more biological properties, provided in broad categories. (cdc.gov)
  • DEC-205 (CD205), a member of the macrophage mannose receptor protein family, is the prototypic endocytic receptor of dendritic cells, whose ligands include phosphorothioated cytosine-guanosine (CpG) oligonucleotides, a motif often seen in bacterial or viral DNA. (uci.edu)
  • Although the membrane structure of the Wilson ATPase has not been experimentally determined, hydropathy plot analysis and site-directed muta-genesis suggests a protein with eight transmembrane domains where the amino and carboxyl terminus are located on the same side of the membrane (Fig. 3). (medscape.com)
  • The six copper-binding domains in the amino-terminus are unique to the subfamily of copper transporters. (medscape.com)
  • The amino terminus contains six homologous domains, each of which contain the copper-binding sequence MXCXXC (Fig. 3). (medscape.com)
  • Human protamine P2 has an amino acid motif at its N-terminus that can serve as a heavy metal trap, especially for Ni(II) and Cu(II). (cdc.gov)
  • Our analysis of genomic neighbourhood of cad and motifs of the encoded polypeptide and its orthologs suggests a close functional association between cAD1 and ApbE protein (Q82Z24), a FMN insertion and trafficking facilitator. (degruyter.com)
  • These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)
  • Two of the regions, called the helix-loop-helix motif and the leucine-zipper motif, are critical for protein interactions. (medlineplus.gov)
  • The newly discovered motifs, especially the clade-specific motifs, should facilitate future functional study of substrate and donor specificity among BAHD enzymes. (biomedcentral.com)
  • Here, we have rationally incorporated vitamin B 6 molecules, featuring alcohol, amine, and phosphate groups on a pyridinyl framework, around a cobaloxime center to generate a first-of-its-kind synthetic catalyst containing non-amino-acid-based biomolecules in the OCS. (rsc.org)
  • We have synthesized a pentadecapeptide modeling this motif, Arg-Thr-His-Gly-Gln-Ser-His-Tyr-Arg-Arg-Arg-His-Cys-Ser-Arg-amide (HP21-15) and described its complexes with Ni(II) and Cu(II), including their capacity to mediate oxidative DNA degradation [Bal et al. (cdc.gov)
  • Presence of the amino acid serine at position 11 (serine 11) in HLA-DRβ1 was associated with an increased risk of uveitis in JIA patients (odds ratio [OR] 2.60, P = 5.43 × 10 -10 ) and was specific to girls (P females = 7.61 × 10 -10 versus P males = 0.18). (nih.gov)
  • Are motifs altered in E. coli? (nih.gov)
  • Members of the F-box protein family, such as FBXO45, are characterized by an approximately 40-amino acid F-box motif. (nih.gov)
  • all 3 amino acids in this motif are in perfect linkage disequilibrium and show identical association with disease. (nih.gov)
  • However, a doubly ( 13 C, 19 F) labeled amino acid should provide greater resolution in two-dimensional ( 19 F, 13 C) NMR spectra, with further possibilities of assignment without mutational analysis. (biorxiv.org)
  • Immunocytochemical, immunoelectron microscopic, and biochemical analyses of rodent neuronal cells showed that a portion of MEF2D was targeted to mitochondria via an N-terminal motif and the chaperone protein mitochondrial heat shock protein 70 (mtHsp70). (nih.gov)
  • Stoichiometry of chemical reactions, quantum mechanical description of atoms, the elements and periodic table, chemical bonding, real and ideal gases, thermochemistry, introduction to thermodynamics and equilibrium, acid-base and solubility equilibria, introduction to oxidation-reduction reactions. (blogspot.com)
  • How Physical Chemistry and Thermodynamics can be used to understand equilibrium balances in nature, with applications to simple organic reactions, acid-base chemistry, solubility and materials, molecular kinetics, and electrochemistry. (blogspot.com)
  • Members of this enzyme family typically share two conserved amino acid motifs. (wikipedia.org)
  • LIG_RPA_C_Plants is similar to the vertebrate motif LIG_RPA_C_Vert , but shorter. (eu.org)
  • The amino acid positions or small motifs that are diagnostic characteristics of DAM5 and DAM6 were determined. (frontiersin.org)
  • After two variable positions, an asparagine or a glutamine is possible in the fifth position, followed by a positive charged amino acid, leucine is also possible, in the sixth position. (eu.org)
  • In addition to implement iceLogo in R to visualize differential amino acid sequence pattern, dagLogo can also test and visualize significant amino acid group patterns by classifying the amino acids into groups according to charge, chemistry and hydrophobicity and etc. (umassmed.edu)
  • Desirable locations for this NMR spin label include the branched chain amino acid methyl groups. (biorxiv.org)
  • We have also utilized ephedrine as a chiral controller in the asymmetric synthesis of alpha hydroxy acids and applied this methodology in the synthesis of alkaloids and marine natural products. (mun.ca)
  • Thus far, only two isoforms of the Wilson ATPase have been identified: the common 1466 amino acid protein expressed in hepatocytes and a splice variant generated by alternate promoter usage and expressed in rat pinealocytes and retina. (medscape.com)