• Adenovirus E1B protein usually refers to one of two proteins transcribed from the E1B gene of the adenovirus: a 55kDa protein and a 19kDa protein. (wikipedia.org)
  • these include the E1B-19KDa protein, which is a homologue of the Bcl-2 protein, the E3-14.7KDa protein (called E3-14.7K) and the E3-14.5KDa/10.4KDa heterodimer. (yu.edu)
  • Recombinant vaccinia virus and adenovirus have been developed as potent vaccine vehicles for treating cancer and infectious diseases. (dukecancerinstitute.org)
  • YKL-1, E1B 55 kDa-deleted recombinant adenovirus vector, capable of harboring a transgene casette of up to 4.9 kb, was newly constructed by reintroducing E1A and E1B 19 kDa into E1/E3-deleted adenoviral vector with a homologous recombination in E. coll. (elsevierpure.com)
  • We have constructed a nondefective recombinant virus between the nononcogenic adenovirus 5 (Ad5) and the highly oncogenic Ad12. (uu.nl)
  • Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. (9lb.cc)
  • Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. (9lb.cc)
  • Recombinant protein expression of interest is detectable from 24 hours post infection. (9lb.cc)
  • To prevent infected cell lysis, recombinant replication deficiency virus has been developed as a gene delivery tool to replace wild-type adenovirus (recombinant AdV in "application" part). (genemedi.com)
  • E1B-55k also forms a complex with E4orf6, a viral protein. (wikipedia.org)
  • The E1B-55k/E4orf6 complex in infected cells assembles with other cellular proteins to form a ubiquitin ligase complex. (wikipedia.org)
  • Essentially, the E1B-55k/E4orf6 complex takes over the cellular ubiquitin ligase complexes and gives them viral substrate-recognition subunits. (wikipedia.org)
  • The MRN complex, if not bound by the E1B-55K/E4orf6 ubiquitin ligase, will treat the ends of the viral DNA like a double-stranded DNA break and the viral DNA becomes ligated into long concatemers of randomly assorted genomes. (wikipedia.org)
  • Chastain-Moore AM, Roberts T, Trott DA, Newbold RF, Ornelles DA , An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression. (coriell.org)
  • Protein-Protein Interactions Facilitate E4orf6-Dependent Regulation of E1B-55K SUMOylation in HAdV-C5 Infection. (leibniz-liv.de)
  • The adenovirus E4orf6 protein inhibits DNA double strand break repair and radiosensitizes human tumor cells in an E1B-55K-independent manner. (wakehealth.edu)
  • Subsequently, the interaction of E4orf3 with four different proteins, DNA-PK, Aup1, E1B-55 kDa and E4orf6 was analyzed in detail by GST-pulldown technique. (metu.edu.tr)
  • In these experiments, E4orf3 was shown to associate with Aup1, E1B-55 kDa and E4orf6 in vitro, and the C-terminal of E4orf3 was determined to be responsible for these interactions. (metu.edu.tr)
  • Together, BAK and BAX form pores in the mitochondrial membrane, releasing apoptogenic proteins like cytochrome c. (wikipedia.org)
  • The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2. (thermofisher.com)
  • In the PARK2-dependent pathway, PINK1 activates PARK2 to target many mitochondrial proteins, including NDP52, OPTN and p62, and it combines with LC3 to deliver damaged mitochondria to autophagosomes [ 15 ]. (ijbs.com)
  • Furthermore, we uncovered that autophagy promotes proline biosynthesis through mitophagy-mediated regulation of NADK2 (NAD kinase 2, mitochondrial), an enzyme responsible for production of mitochondrial NADP(H). Using an orthotopic mouse model of PDAC, we found that inhibiting mitophagy by targeting PRKN (parkin RBR E3 ubiquitin protein ligase) in the stroma reduced tumor weight. (bvsalud.org)
  • BNIP3 and NIX are related multi-functional outer mitochondrial membrane proteins. (1library.net)
  • Here, after a general discussion of mitophagy, we will review and discuss two related outer mitochondrial membrane (OMM) proteins, BNIP3 and BNIP3L (NIX). (1library.net)
  • Mechanistically, OA dose-dependently induced the expression of Bcl-2/adenovirus E1B 19-kDa interacting protein (BNIP3), while knockdown of BNIP3 expression reversed the protection of OA against HR-mediated mitochondrial injury. (figshare.com)
  • Observations show that E1b-55k inhibits activation by p53 by binding a repression domain to it, converting it from an activator to a repressor of p53-activated genes. (wikipedia.org)
  • Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. (lookformedical.com)
  • The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS. (lookformedical.com)
  • The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. (9lb.cc)
  • NAC-type plant-specific transcription factor genes encode proteins that play important roles in abiotic stress responses, as well as regulation of plant development. (metu.edu.tr)
  • Top panel: a simplified map of the adenovirus serotype 5 genome showing the early genes (E1-E4) and the region from which the major late transcript is produced. (genemedi.com)
  • E1B-55K-Mediated Regulation of RNF4 SUMO-Targeted Ubiquitin Ligase Promotes Human Adenovirus Gene Expression. (tum.de)
  • Human Adenovirus Infection Causes Cellular E3 Ubiquitin Ligase MKRN1 Degradation Involving the Viral Core Protein pVII. (tum.de)
  • The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can't be integrated into host genome, guaranteeing the security for subsequent operations. (9lb.cc)
  • Transduction results of various Adenovirus vectors. (9lb.cc)
  • Therefore, understanding the functions of E4orf3 is very important for developing efficient and safe adenovirus vectors. (metu.edu.tr)
  • Schematic of the adenovirus genome and adenovirus-based vectors. (genemedi.com)
  • Without E1B-19k, degradation of both cellular and viral DNA occurs, in addition to premature host cell death during the lytic cycle, thus limiting viral replication. (wikipedia.org)
  • A Single Amino Acid Switch in the Adenoviral DNA Binding Protein Abrogates Replication Center Formation and Productive Viral Infection. (leibniz-liv.de)
  • Adipocyte commitment of 3T3-L1 cells is required to support human adenovirus 36 productive replication concurrent with altered lipid and glucose metabolism. (leibniz-liv.de)
  • Evidence that the Adenovirus Single-Stranded DNA Binding Protein Mediates the Assembly of Biomolecular Condensates to Form Viral Replication Compartments. (leibniz-liv.de)
  • Viral DNA Binding Protein SUMOylation Promotes PML Nuclear Body Localization Next to Viral Replication Centers. (leibniz-liv.de)
  • Simply, the E1 gene products are early proteins that are transcribed in the early transcribed regions and required for proceeding subsequent steps in viral replication. (9lb.cc)
  • The E1 gene contains E1A and E1B, involved in the replication of adenovirus. (9lb.cc)
  • E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters. (9lb.cc)
  • Bronchial epithelial cells (BEAS-2B cells and primary bronchial epithelial cells) were exposed to heat-inactivated Haemophilus influenzae , Pseudomonas aeruginosa or Streptococcus pneumoniae and subsequently infected with respiratory syncytial virus (RSV), type 2 human adenovirus or influenza B. Levels of pro-inflammatory cytokines, viral replication and expression of pattern recognition receptors were determined in culture supernatants and/or cell lysates. (biomedcentral.com)
  • Adenovirus transcription is a two-phase event, early and late, occurring before and after viral DNA replication, respectively (Figure. (genemedi.com)
  • Life cycle of adenovirus is separated by DNA replication process into two distinct phases: the early and late, occurring before and after viral DNA replication, respectively. (genemedi.com)
  • daughter of AQ-1, had a febrile respiratory infection with Human Adenovirus Type 55 cough. (cdc.gov)
  • E1B-55K is a phosphorylation-dependent transcriptional and post-transcriptional regulator of viral gene expression in HAdV-C5 infection. (leibniz-liv.de)
  • Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein. (leibniz-liv.de)
  • Rather, the localization of p53 to E1B containing nuclear sites observed during infection by Ad5 was prevented by mutations that impair interaction of the E1B protein with p53 and/or with the E4 Orf6 protein. (princeton.edu)
  • Using a subviral particle vaccine candidate, we show here that one protein complex of HCMV, termed the pentameric complex (PC), enhances the neutralizing antibody response against viral infection of different cell types. (asm.org)
  • Additionally, deletion from the RK3616 gene inside the HSV-1 virus inhibited the downstream phosphatase, PKR (dsRNA induced protein kinase), producing normal cells resistant to infection whilst leaving tumorigenic cells vulnerable as a consequence of disturbance of cellular antiviral pathways [257]. (estrogen-receptor.com)
  • For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
  • Infection process of adenovirus. (genemedi.com)
  • The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)
  • BNIP3 (Bcl-2/ adenovirus E1B 19-kDa-interacting protein 3) is a receptor accounting for priming damaged mitochondria for autophagic removal. (chinaphar.com)
  • BCL2 and adenovirus E1B 19-kDa interacting protein 3 (BNIP3), a well-known atrogene, was identified as a potential mediator of muscle fiber atrophy in LOPD muscle biopsies. (nih.gov)
  • The current data suggested that BNIP3 expression is regulated by inhibition of the AKT-mammalian target of rapamycin pathway, leading to phosphorylation of Unc-51 like autophagy activating kinase 1 (ULK1) at Ser317 by AMP-activated protein kinase. (nih.gov)
  • These paclitaxel resistant ovarian cells demonstrate: (1) Increased IC 50 for paclitaxel and docetaxel (10 to 75-fold) and cross-resistance to anthracyclines (2) Reduced cell apoptosis in the presence of paclitaxel (3) Gene depletion involving mitotic regulators BUB1 mitotic checkpoint serine/threonine kinase, cyclin BI (CCNB1), centromere protein E (CENPE), and centromere protein F (CENPF), and (4) Functional data validating gene depletion among mitotic regulators. (biomedcentral.com)
  • Presence of viral protein at both 37 h and 7 days was confirmed by immunohistochemistry using antibodies directed against hexon, penton, and fiber proteins. (elsevierpure.com)
  • It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. (thermofisher.com)
  • Rak, M. A., J. Buehler, S. Zeltzer, J. Reitsma, B. Molina, S. Terhune, and F. Goodrum, 'Human Cytomegalovirus UL135 Interacts with Host Adaptor Proteins To Regulate Epidermal Growth Factor Receptor and Reactivation from Latency. (arizona.edu)
  • Without integration into host genome, adenovirus genome remains in an episomal state, which guarantees the low risk of mutation (Fig. 8). (genemedi.com)
  • Specifically, we uncovered a role for nucleosides, which are secreted by CAFs through autophagy in a nuclear fragile X mental retardation-interacting protein 1 (NUFIP1)-dependent manner, increased glucose utilization and promoted growth of PDAC. (bvsalud.org)
  • Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. (affbiotech.cn)
  • Nuclear Factor 90 Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
  • In addition to binding to RNA, nuclear factor 90 proteins form heterodimeric complexes that regulate GENETIC TRANSCRIPTION and may play a role in T-CELL activation. (ouhsc.edu)
  • This graph shows the total number of publications written about "Nuclear Factor 90 Proteins" by people in this website by year, and whether "Nuclear Factor 90 Proteins" was a major or minor topic of these publications. (ouhsc.edu)
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  • This and other proteins released from the mitochondria lead to activation of caspase-9 and caspase-3 and the resulting apoptotic program. (wikipedia.org)
  • The Bcl-2 homology 3 (BH3) domain is crucial for the death-inducing and dimerization properties of pro-apoptotic members of the Bcl-2 protein family, including Bak, Bax, and Bad. (lookformedical.com)
  • Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression. (nih.gov)
  • Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL). (lookformedical.com)
  • Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need. (9lb.cc)
  • To investigate this issue, we examined properties of p53 in normal human cells infected by E1B mutant viruses that display defective entry into the late phase or viral late mRNA export. (princeton.edu)
  • Genomic and phylogenetic analysis of two guinea pig adenovirus strains recovered from archival lung tissue. (leibniz-liv.de)
  • Transverse chest CT images demonstrated in- We identified a case of fatal acute respiratory disease creased areas of patchy shadows and consolidation in both from household transmission of human adenovirus type lungs compared to CT images from April 22, indicative of 55 (HAdV-55) in Anhui Province, China. (cdc.gov)
  • Lee, S. Hee, K. Caviness, E. R. Albright, J-H. Lee, C. B. Gelbmann, M. Rak, F. Goodrum, and R. F. Kalejta, 'Long and Short Isoforms of the Human Cytomegalovirus UL138 Protein Silence IE Transcription and Promote Latency. (arizona.edu)
  • Conserved E1B-55K SUMOylation in different human adenovirus species is a potent regulator of intracellular localization. (leibniz-liv.de)
  • Animal Models in Human Adenovirus Research. (leibniz-liv.de)
  • KIR3DS1 directs NK cell-mediated protection against human adenovirus infections. (leibniz-liv.de)
  • It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. (princeton.edu)
  • These results indicate that the E1B protein fulfills an early function that correlates efficient entry into the late phase with the localization of E1B and p53 in the nucleus of Ad5-infected normal human cells. (princeton.edu)
  • Human Adenovirus Core Protein V Is Targeted by the Host SUMOylation Machinery To Limit Essential Viral Functions. (tum.de)
  • All four FIPs are novel proteins and are expressed in all major human tissues assayed. (yu.edu)
  • Human telomerase reverse transcriptase (hTERT), epithelial development aspect receptor, and survivin are normally active and overexpressed proteins within a variety of tumor types [259]. (estrogen-receptor.com)
  • Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. (genemedi.com)
  • E1B proteins work to prevent apoptosis that is induced by the small adenovirus E1A protein, which stabilizes p53, a tumor suppressor. (wikipedia.org)
  • In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. (utsouthwestern.edu)
  • You will find particular proteins with indicated tumor-type specificity: prostate certain antigen in prostate tumors, tyrosinase for melanomas, estrogen receptor protein and foetoprotein for hepatocarcinomas [258]. (estrogen-receptor.com)
  • In this study, structural and functional aspects of adenovirus type 5 (Ad5) E4orf3 protein were analyzed by biophysical and biochemical methods. (metu.edu.tr)
  • Proteins found in the nucleus of a cell. (lookformedical.com)
  • Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus. (lookformedical.com)
  • In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (jefferson.edu)
  • Then the endosome acidifies, resulting in disassociation of capsid proteins and transportation of viral DNA into nucleus. (genemedi.com)
  • In the CYTOPLASM, I-kappa B proteins bind to the transcription factor NF-KAPPA B. Cell stimulation causes its dissociation and translocation of active NF-kappa B to the nucleus. (bvsalud.org)
  • Hexon and penton structures form the capsid of AdV, and fiber protein mediates the binding of the virion to the cell surface and is a major determinant of viral tropism. (genemedi.com)
  • Structural and bioinformatics studies have shown that E1B-55k, which is specific to mammalian mastadenoviruses, has evolved by exaptation from an LH3-like minor capsid protein encoded by atadenoviruses. (wikipedia.org)
  • Mutagenesis analysis has shown that the GTP-binding domain of FIP-1 is indispensable for its interaction with E3-14.7K. FIP-1 is very similar to two proteins: (1) an S. cerevisiae GTP-binding protein that presumably has a role in phosphate transport and (2) a C. elegans protein of unknown function. (yu.edu)
  • CAR receptor-fiber-knob adenovirus interaction and internalization process [46]. (genemedi.com)
  • E1B-19k mimics MCL1, which is a cellular antiapoptotic protein. (wikipedia.org)
  • However, in adenovirus-infected cells, activated BAK and BAX are sequestered by E1B-19k, preventing the pathway. (wikipedia.org)
  • The Wnt pathway contains quite a few other proteins typically implicated in many types of tumors, particularly inside the stomach and intestines. (estrogen-receptor.com)
  • Adenoviruses have already been modified to particularly target this pathway, even though with mixed success [259,260]. (estrogen-receptor.com)
  • TNF-{dollar}\alpha{dollar} induced rapid but transient phosphorylation and association of FIP-1 with other cellular proteins. (yu.edu)
  • The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. (utsouthwestern.edu)
  • Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. (utsouthwestern.edu)
  • Our results also suggest that E3-14.7K is acting at steps or pathways of TNF-{dollar}\alpha{dollar} cytolysis different from the ones affected by E1B-19K or Bcl-2 since none of the FIPs interact with either Bcl-2 or ElB-19K. (yu.edu)
  • Immunoglobulin J Recombination Signal Sequence-Binding Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • A ubiquitously expressed sequence-specific transcriptional repressor that is normally the target of signaling by NOTCH PROTEINS. (harvard.edu)
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  • The steady-state concentrations of p53 were significantly higher in cells infected by the E1B 55 kDa null mutant Hr6 or three mutants carrying small insertions in the E1B 55 kDa protein coding sequence than in Ad5-infected cells. (princeton.edu)
  • One such study demonstrated that an E1B-19kDa gene deletion mutant enhanced cancer specificity by means of TNF pathways, substantially enhancing viral spread and antitumoral capacity whilst simultaneously sustaining selectivity [259]. (estrogen-receptor.com)
  • However, some of its proteins possess oncogenic potential and their presence comprises safety risks. (metu.edu.tr)
  • The viral pentameric protein complex consisting of glycoprotein H (gH)/gL/UL128-131A (PC) is considered to be an important vaccine component. (asm.org)
  • A maternal high-fat diet modulates fetal SIRT1 histone and protein deacetylase activity in nonhuman primates. (childrensmercy.org)
  • These data show that tumor induction by adenovirus virions is not determined only by the gene products of the transforming region. (uu.nl)
  • Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. (lookformedical.com)