Adenovirus e1 proteins. Medical search. Frequent questions

E1-deleted (including E1a-deleted) adenoviruses are replication defective and are commonly used as viral vectors to carry therapeutic genes for gene therapy. (hindawi.com)
Adenovirus vectors are widely used in gene therapy and vaccine development due to their ability to efficiently deliver genes and antigens to target cells. (inventiv.org)
The use of adenovirus vectors for multiple vaccinations is particularly attractive because they can be engineered to express multiple antigens, which can provide protection against multiple diseases. (inventiv.org)
The global market for adenovirus vectors is expected to grow at a compound annual growth rate (CAGR) of 9.9% from 2020 to 2027. (inventiv.org)
Several companies are actively engaged in the development of adenovirus vectors for multiple vaccinations. (inventiv.org)
The market for adenovirus vectors for multiple vaccinations is also witnessing significant investments from governments and private organizations. (inventiv.org)
Methods to generate immune responses using the adenovirus Vectors are provided that allow multiple vaccinations with one adenovirus virus vector, and vaccinations of individuals with preexisting immunity. (inventiv.org)
The invention concerns methods of generating immune responses using Adenovirus vectors that allow multiple vaccination regimens. (inventiv.org)
The greatest problem with adenovirus-based vectors is their inability to maintain long-term transgene expression. (inventiv.org)
We recommend aliquoting your vectors into low protein binding tubes upon receipt. (vectorbiolabs.com)
The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can't be integrated into host genome, guaranteeing the security for subsequent operations. (9lb.cc)
Transduction results of various Adenovirus vectors. (9lb.cc)
c production of replication incompetent AAV vectors via transient plasmid transfection into 293 producer cells that express adenovirus type 5 E1 genes. (biomedcentral.com)
Thus, our data show that E2F-binding sites in the E2-early promoter are crucial for E1A independent adenoviral DNA replication of E1-deleted vectors in cancer cells. (bvsalud.org)
IMPORTANCE E1-deleted AdV vectors are considered replication deficient and are important tools for the study of virus biology, gene therapy, and large-scale vaccine development. (bvsalud.org)
Schematic of the adenovirus genome and adenovirus-based vectors. (genemedi.com)
pAd1129-01 is a shuttle plasmid designed for constructing E3-deleted and bipartite (dicistronic) recombinant adenovirus vectors. (od260.com)
Intro Adenovirus vectors are believed a respected viral vector system for vaccines for their powerful immunogenicity and making feasibility. (synanet2020.com)
The strongest adenovirus vectors for make use of as vaccines derive from adenovirus serotype 5 (Advertisement5) (1C6). (synanet2020.com)
Adenovirus-based vectors can handle generating powerful and protecting T cell and antibody reactions in animal versions (7C17), and medical data conclusively display that Advertisement5 vectors can induce powerful Compact disc8+ and Compact disc4+ T cell and antibody reactions in vaccinated volunteers (18C25). (synanet2020.com)
They support the development of E1/E4-removed vectors predicated on Advertisement5 aswell as choice adenovirus serotypes (43C46). (synanet2020.com)
300 pre-made and ready to use adenovirus vectors. (signagen.com)

Top panel: a simplified map of the adenovirus serotype 5 genome showing the early genes (E1-E4) and the region from which the major late transcript is produced. (genemedi.com)
The index case and a further individual were diagnosed with an adenovirus serotype 4 co-infection. (biomedcentral.com)
All the pre-packaged adenoviruses were packaged from Ad.MAX™ system with a serotype 5 adenoviral backbone* (E1/E3 deletion). (signagen.com)

It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
In Utrecht Sussenbach and Van der Vliet studied the fate of adenovirus DNA in the host cells and the mechanisms that adenoviruses use to replicate their DNA genome. (scienceexhibitions.org)
The recombinant genome consists essentially of Ad5 sequences, with the exception of the transforming early region 1 (E1) which is derived from Ad12. (uu.nl)
Without integration into host genome, adenovirus genome remains in an episomal state, which guarantees the low risk of mutation (Fig. 8). (genemedi.com)
When you construct a recombinant adenovirus genome in E. coli using cosmid technology, you are almost sure that every colony carries a full-size genome, and not a smaller plasmid that lost chuncks of the adenovirus genome by DNA recombination. (od260.com)
Both of them are flanking both ends of the recombinant adenovirus genome contained in your cosmid. (od260.com)
Because the PacI site is immediately flanking the start of the adenovirus genome, the DNA ends generated by PacI resemble the most the ends obtained from deproteinised virion DNA and might therefore be more efficient in promoting virus replication. (od260.com)
2003). The 12.5 kb CSFV genome contains a single open reading frame that encodes a 3898-amino acid polyprotein and ultimately yields 11 to 12 final cleavage products (NH2-N^pro-C-E^rns-E1-E2-p7-NS2-NS3-NS4A-NS4B-NS5A-NS5B-COOH) through co- and post-translational processing of the polyprotein by cellular and viral proteases (Rice, 1996). (usda.gov)
Ad.MAX™ Technology for Maximum Adenovirus Production: Ad.MAX™ technology was developed by genetically modifying virus packaging cell, HEK293 cell and adenoviral shuttle vector or adenoviral genome for maximum adenovirus production. (signagen.com)

To test this hypothesis, we developed an in vivo model of VEGF overexpression in the lung on the basis of delivery to the respiratory epithelium of the VEGF165 complementary DNA by an E1(-) adenovirus vector (AdVEGF165). (nih.gov)
To confirm the specificity of these observations, mice were pretreated with intranasal administration of an adenovirus vector expressing a truncated soluble form of the VEGF receptor flt-1 (Adsflt). (nih.gov)
Adsflt (10(9) pfu) pretreatment completely abrogated the increased lung wet/dry weight ratio caused by AdVEGF165 administration, whereas an identical adenovirus vector with an irrelevant transgene had no effect upon subsequent AdVEGF165-induced pulmonary edema. (nih.gov)
The market for methods, compositions, and methods for creating an adenovirus virus vector for multiple vaccinations is rapidly growing. (inventiv.org)
For instance, Vaxart Inc. is developing an oral adenovirus vector-based vaccine for COVID-19 that can provide long-lasting immunity against the virus. (inventiv.org)
Another company, GenVec Inc., is developing an adenovirus vector-based vaccine for the prevention of Ebola virus disease. (inventiv.org)
For instance, the Coalition for Epidemic Preparedness Innovations (CEPI) has invested $8.4 million in the development of an adenovirus vector-based vaccine for COVID-19. (inventiv.org)
In conclusion, the market for methods, compositions, and methods for creating an adenovirus virus vector for multiple vaccinations is witnessing significant growth due to the increasing prevalence of infectious diseases, the growing demand for gene therapy, and the rising investments in research and development activities. (inventiv.org)
With the development of more effective and safe adenovirus vector-based vaccines, the market is expected to witness further growth in the coming years. (inventiv.org)
Preexisting or inducible immunity to vaccines against adenovirus (Ad), limits the use of First Generation vector vaccines. (inventiv.org)
Another study showed that an adenovirus vector vaccine containing an HIV-1 envelope gene was incapable in reimmunizing primed immune responses using non-adjuvanted genetic DNA. (inventiv.org)
Preexisting immunity can interfere with the effectiveness of adenovirus vector vaccinations. (inventiv.org)
An E1/E3 deleted adenovirus type 5 vector expressing cre recombinase under CMV promoter control. (umich.edu)
E1 and partial E3 deleted adenoviral vector, RSV LTR promoter, and expresses human Bclx short (BclxS). (umich.edu)
E1/E3 deleted adenoviral reporter vector, CMV promoter, expresses green fluorescent protein. (umich.edu)
Adenoviral reporter vector, E1/E3 deleted, CMV promoter, expresses nuclear targeted β-galactosidase (LacZ). (umich.edu)
Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. (9lb.cc)
discovered to correlate using the differential ramifications of the adenovirus E1 proteins for the manifestation of mobile MHC-class I substances (Bernards gene into hematopoietic stem cells (Hoogerbrugge shown an adenovirus vector missing binding sites for indigenous receptors blood-clotting elements and complement parts in its capsid. (scienceexhibitions.org)
As a result the vector exhibited reduced capability to bind human being erythrocytes strongly decreased hepatic tropism and improved bioavailability upon systemic delivery to mice (Schagen (2014) who proven the potential of utilizing T-cell-derived Jurkat cells to provide oncolytic adenovirus towards the primary and infiltrative areas of intracranial glioma in mice therefore opening potential for potential autologous T-cell-mediated oncolytic pathogen delivery. (scienceexhibitions.org)
The vaccine was a replication-defective E1/E3 deleted adenovirus (Ad5) vector expressing antigens consisting of 16 multimers of GnRH, bacterial leukotoxin, T-helper epitopes, and other various hinge and linker amino acids (Ad-GnRH). (auburn.edu)
This study demonstrates that mares are capable of developing an anti-GnRH antibody response to homologous immunization using a replication-defective E1/E3 deleted replication-defective adenovirus vector encoding GnRH peptide, bacterial leukotoxin, and T-helper epitopes. (auburn.edu)
Middle panel: general structure of an early region 1 (E1)-deleted Ad vector. (genemedi.com)
We hypothesized that an Ad5 vector containing the nonhuman CAV2 knob would provide expanded tropism and constructed Ad5Luc1-CK, an E1-deleted Ad5 vector encoding the fiber knob domain from CAV2. (cnrs.fr)
Sputnik V is a two-part adenovirus viral vector vaccine with an efficacy rate of 91.6% . (medicalnewstoday.com)
An invasive Escherichia coli expressing the inv gene from Yersinia pseudotuberculosis was used as a vector for protein delivery to mammalian epithelial cells. (shengsci.com)

The adenovirus E1A-associated 130-kD protein is encoded by a member of the retinoblastoma gene family and physically interacts with cyclins A and E". Genes Dev. (wikipedia.org)
The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein. (9lb.cc)
These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease. (biomedcentral.com)
However, deletion of the E1 genes does not completely abolish viral DNA replication in cancer cells. (bvsalud.org)
recently conducted phylogenetic analysis of viral protein (VP) 3-VP1 genes, which revealed greater genetic variability of HCoSV strains, and proposed splitting species A into 24 species (A1-A24), D into 5 species (D1-D5), E into 2 species (E1-E2), and classifying F as 1 species ( 6 ). (cdc.gov)

In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)

The best PSP was selected to construct a prostate-specific oncolytic adenovirus (CRAD) by controlling the adenoviral E1 region. (hindawi.com)
Another clinical trial in which an oncolytic adenovirus was equipped with (Li. (scienceexhibitions.org)

T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
Recombinant adenovirus vaccines should therefore aim at replicating the naturally occurring PPRV immunity. (biomedcentral.com)
When used in vaccines, adenoviruses are altered to remove their ability to replicate. (medicalnewstoday.com)

Immunization strategies against this morbillivirus using recombinant adenoviruses expressing PPRV-F or -H proteins can protect PPRV-challenged animals and permit differentiation of infected from vaccinated animals. (biomedcentral.com)
Cosmids are really great tools to construct recombinant adenoviruses because their minimum sizes (~39 kb) accommodates the 36 kb adenovirus genomes almost perfectly. (od260.com)

Recombinant protein expression of interest is detectable from 24 hours post infection. (9lb.cc)
A Single Amino Acid Switch in the Adenoviral DNA Binding Protein Abrogates Replication Center Formation and Productive Viral Infection. (leibniz-liv.de)
Protein-Protein Interactions Facilitate E4orf6-Dependent Regulation of E1B-55K SUMOylation in HAdV-C5 Infection. (leibniz-liv.de)
Human Adenovirus Type 5 Infection Leads to Nuclear Envelope Destabilization and Membrane Permeability Independently of Adenovirus Death Protein. (leibniz-liv.de)
For most serotypes, adenovirus infection is mediated by the high-affinity binding of the fiber-knob region to a receptor of target cell, named as the coxsackie-Ad receptor (CAR), which mainly determines the viral tropism [44]. (genemedi.com)
Infection process of adenovirus. (genemedi.com)
The relevance of the adenovirus co-infection remains unclear and merits further investigation. (biomedcentral.com)
Proof also shows that tyrosine phosphorylation is normally involved with HSV-1 replication because (we) HSV-1 penetration sets off tyrosine phosphorylation of mobile protein (1, 35), (ii) many viral protein are tyrosine phosphorylated during an infection (3, 30), and (iii) HSV-1 replication is normally inhibited in the current presence of tyrosine kinase inhibitors (13, 44C46). (thebiotechdictionary.com)
This then triggers the production of antibodies against this spike protein, preparing the immune system for a potential infection. (medicalnewstoday.com)
Here, we report the effect of IFN-alpha treatment (delivered by a replication defective human adenovirus type 5, Ad5) on the course of experimental infection of swine infected with the highly virulent CSFV Brescia strain. (usda.gov)
Within 12h of infection and before start of DNA replication, there begins early protein synthesis. (biologydiscussion.com)
Mutant line R3-2 exhibited a nearly 1000-fold resistance to infection by genetically marked Moloney murine leukemia virus genomes, and was resistant to pseudotyped viruses utilizing the ecotropic envelope, the amphotropic envelope, or even the VSV G envelope protein. (biomedcentral.com)

Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus. (9lb.cc)
Also, bovine herpesvirus 1 (BHV-1) glycoprotein E (gE) is normally tyrosine phosphorylated during viral replication as well as the titer of trojan is normally proportional to the amount of phosphorylation of the envelope proteins (39). (thebiotechdictionary.com)
Multi Approaches to Produce Super Infectious rAAV Particles at Super High Titer: Not like adenovirus, it is relatively harder to achieve high yield of rAAV with regular rAAV production procedures. (signagen.com)

A number of PSPs have been defined over the years that include, but not limited to, promoters of prostate-specific antigen (PSA), probasin (PB), mouse mammary tumor virus (MMTV LTR), prostate-specific membrane antigen (PSMA), human glandular kallikrein 2 (hK2), and prostatic steroid-binding protein C3. (hindawi.com)
Vehicle den Noordaa Clinofibrate in Amsterdam and Vehicle der Eb in Leiden utilized adenovirus and SV40 as tumor-inducing real estate agents in rodent versions. (scienceexhibitions.org)
Although very clear advances are becoming produced toward oncolytic adenoviruses you can use systemically clinical execution of systemic tumor treatment with oncolytic infections is not however being considered in the Netherlands. (scienceexhibitions.org)
These data show that tumor induction by adenovirus virions is not determined only by the gene products of the transforming region. (uu.nl)
In the present study, we utilized adenovirus expressing the em NK4 /em gene, which has previously been demonstrated to suppress tumor growth and vascularization in Diflumidone mice [14]. (demotivate.org)

The idea behind this study is to place the Ad5 E1 region in cis complementation (i.e., use E1 as a transgene) back into an E1-deleted, replication-defective adenovirus under the control of a prostate-specific promoter (PSP). (hindawi.com)
E1/E3 deleted, CMV promoter, expressing human p53 wild type. (umich.edu)
E1 and partial E3 deleted with CMV promoter. (umich.edu)
E1 and partial E3 deleted, RSV promoter, expressing constituently active transforming growth factor beta. (umich.edu)
The inactivating effect of sequence-specific promoter methylations was extensively studied by using the late E2A promoter of adenovirus type 2 (Ad2) DNA. (uni-bielefeld.de)
It was demonstrated in transient expression experiments that the 5' CCGG 3'-methylated late E2A promoter was at least partly reactivated in cell lines constitutively expressing the E1 region of Ad2 or of adenovirus type 5 (Ad5) DNA. (uni-bielefeld.de)
These findings implicated the E1A 289 amino acid residue protein of Ad2, a well-known transactivator, as the reactivating function of the endogenous, previously dormant, late E2A promoter-CAT gene assembly. (uni-bielefeld.de)

Another study reported that nonhuman primates with pre-existing immunity to Ad5 were unable, after a single Ad5 immunization, to produce transgene-specific antibodies against HIV proteins. (inventiv.org)

The AAV capsid protein, which forms the virions' outer shell, is the primary determinant of tissue tropism, transduction efficiency, and immunogenicity. (biomedcentral.com)
On the other hand, the quantity of HSV-1 tegument proteins UL37 in virions is normally strictly handled despite a 20-fold boost of UL37 in contaminated cells (25). (thebiotechdictionary.com)

The adenovirus early region 1 (E1) gene, which comprises E1a and E1b, encodes the viral early proteins that are necessary for adenoviral replication and the consequent oncolysis of permissive host cells. (hindawi.com)
The dsDNA encodes the early proteins and capsid proteins. (biologydiscussion.com)

This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. (wikipedia.org)
Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues. (9lb.cc)
Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C). (9lb.cc)
He S , Zhang H , Lu Y , Zhang Z , Zhang X , Zhou N , Hu Z , . Nampt promotes osteogenic differentiation and lipopolysaccharide-induced interleukin-6 secretion in osteoblastic MC3T3-E1 cells. (aging-us.com)
This study aimed to explore the effect of Nampt in osteogenic differentiation and inflammatory response of osteoblastic MC3T3-E1 cells. (aging-us.com)
We developed an in vitro model of lipopolysaccharide (LPS)-induced inflammation and showed that Nampt and Sirt1 were significantly upregulated in LPS-treated MC3T3-E1 cells. (aging-us.com)
Then we transfected cells with adenoviruses to knock down or over express Nampt. (aging-us.com)
Therefore, we demonstrated that Nampt acted both in promoting osteoblast differentiation and in enhancing inflammatory response, mediated by Sirt1 in MC3T3-E1 cells. (aging-us.com)
DNA replication of E1-deleted first-generation adenoviruses (AdV) in cultured cancer cells has been reported repeatedly and it was suggested that certain cellular proteins could functionally compensate for E1A, leading to the expression of the early region 2 (E2)-encoded proteins and subsequently virus replication. (bvsalud.org)
These studies identified the role of the human transcription factors NF-I and NF-III/OCT1 in orchestrating the initiation complex involved in adenovirus type 5 DNA replication illustrating the interplay between cellular and viral processes involved with adenovirus replication [evaluated in (Hoeben and Uil 2013 The observations that some infections could change cells were interesting and stimulated research to Clinofibrate acquire insights in the procedures involved in mobile transformation. (scienceexhibitions.org)
Graham and Vehicle der Eb proven that human being cells too could possibly be changed by nude adenovirus DNA utilizing their Clinofibrate calcium mineral phosphate co-precipitation way of the effective transfer of nude DNA into Clinofibrate focus on cells (Graham and vehicle der Eb 1973 Subsequently these methods were used to review the variations in oncogenic potential from the adenovirus types 5 and 12. (scienceexhibitions.org)
Adipocyte commitment of 3T3-L1 cells is required to support human adenovirus 36 productive replication concurrent with altered lipid and glucose metabolism. (leibniz-liv.de)
We found that first-generation adenovirus [E1-, E3-] could infect cochlear hair cells, yet was ototoxic, similar to the toxicity observed in cultured hair cells. (rochester.edu)
However, we determined that a modified adenovirus [E1-,E3-, E2b-] could infect cochlear hair cells both in culture and in vivo with no loss of either transduction currents or cochlear function. (rochester.edu)
Once packaged into a E1-complementing cell line, which provides the E1 products in trans, such as QBI 293A Cells, recombinant viral will be easily propagated. (genemedi.com)
Both mobile and virally encoded kinases get excited about the phosphorylation of tegument protein (5, 11, 12), and serines of tegument proteins HSV-1 VP22 are phosphorylated in contaminated cells (11, 12). (thebiotechdictionary.com)
VP22 relocates to a book subcellular site with another tegument proteins, VP16, in coexpressing cells (7). (thebiotechdictionary.com)
Recombinant NK4 (rNK4) proteins inhibited IFN-, IL-4 and IL-17 production by CD4+ T cells stimulated with allogeneic spleen cells. (demotivate.org)
293-ORF6 cells exhibit both Advertisement5 early area 1 (E1) and E4 gene items (43). (synanet2020.com)
Before the replication begins, early proteins are synthesized in the nucleus of the infected cells. (biologydiscussion.com)
Our group and others have previously shown the potential of this approach for the dominantly inherited neurological disease DYT1 dystonia by achieving potent short-hairpin RNA (shRNA)-mediated silencing of the disease protein, torsinA, in cultured cells. (shengsci.com)
We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
It was described that hedgehog proteins right act on osteogenic precursor cells and osteoblasts to stimulate osteogenic differentiation [7]. (acthreceptor.com)

Hexon and penton structures form the capsid of AdV, and fiber protein mediates the binding of the virion to the cell surface and is a major determinant of viral tropism. (genemedi.com)
Then the endosome acidifies, resulting in disassociation of capsid proteins and transportation of viral DNA into nucleus. (genemedi.com)
The tegument keeps its structural integrity in the lack of the envelope and capsid, indicating solid intermolecular interactions that has to can be found between these tegument proteins to aid the apparently amorphous framework (7, 27, 40). (thebiotechdictionary.com)

In a rat model Idema (2011) demonstrated that distribution of adenovirus particles delivered via CED is at least partially volume dependent when the virus was infused in white matter tracts but not in the gray matter. (scienceexhibitions.org)
According to Anvisa , the Brazilian Health Regulatory Agency, quality control documents revealed that batches of the vaccine displayed "less than 100 replication-competent particles per dose," suggesting that the adenovirus does have a small chance of replication. (medicalnewstoday.com)

More than 100 serologically distinct types of adenovirus have been identified including 49 types that infect humans. (biologydiscussion.com)

Differential Regulation of Cellular FAM111B by Human Adenovirus C Type 5 E1 Oncogenes. (leibniz-liv.de)
To prevent infected cell lysis, recombinant replication deficiency virus has been developed as a gene delivery tool to replace wild-type adenovirus (recombinant AdV in "application" part). (genemedi.com)
The Toronto strain of canine adenovirus type 2 (CAV2) exhibits native tropism that is augmented by, but not fully dependent upon, CAR for cellular transduction. (cnrs.fr)
The incorporation of herpes virus type 1 (HSV-1) tegument proteins VP22 is normally increased a lot more than twofold when the VP22 proteins appearance level is normally elevated fivefold (21). (thebiotechdictionary.com)
Adenoviruses are a type of virus associated with the common cold and other illnesses. (medicalnewstoday.com)
Each of the two doses uses a different type of adenovirus: first dose with type-26 (Ad26), then a booster dose with type-5 (Ad5). (medicalnewstoday.com)
a threonine-to-methionine amino acidity substitution at the positioning equal to residue 342 from the wild-type proteins. (synanet2020.com)
These NAbs focus on the nine hypervariable areas that type the exposed surface area from the hexon proteins (31, 33). (synanet2020.com)

1. Why does adenovirus have a relatively higher immunogenecity compared with rAAV? (9lb.cc)

The vaccine is designed to express the spike protein of the virus, which can stimulate the immune system to produce neutralizing antibodies. (inventiv.org)
Protein vaccine control mares developed anti-GnRH antibodies after vaccination and the response was maintained for 15 weeks. (auburn.edu)
The purpose of using two different types is to lower the possibility of the body developing antibodies against the adenovirus after the first dose, which could make the second dose ineffective. (medicalnewstoday.com)
Two monoclonal antibodies raised against Sindbis virus were shown to be specific for the envelope glycoprotein E1 by radioimmunoprecipitation (RIP). (microbiologyresearch.org)

Papovaviruses are one of the four important dsDNA viruses (e.g. papovaviruses, adenoviruses, herpes viruses and pox viruses) which produce tumour in many animals. (biologydiscussion.com)

Genomic and phylogenetic analysis of two guinea pig adenovirus strains recovered from archival lung tissue. (leibniz-liv.de)

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. (wikipedia.org)
The adenoviruses are common pathogens of humans and animals. (biologydiscussion.com)

When amplifying purified adenovirus that has been serially propagated it may be wise to isolate viral clones (with a plaque amplification), in order to avoid any possible problems with replication-competent adenovirus. (umich.edu)

Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. (9lb.cc)

We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell differentiation. (biomedcentral.com)
An additional objective was to determine the effects of a heterologous vaccination strategy against gonadotropin releasing hormone on estrus behavior and cyclicity in mares using an adenoviral vectored gonadotropin releasing hormone vaccine to prime, and a protein based gonadotropin releasing hormone vaccine to boost. (auburn.edu)
During phase two, mares that had been vaccinated during phase one (previous ovulatory season) were administered a single vaccination using a quarter of the labeled dose (sub-effective) of a protein based gonadotropin releasing hormone vaccine (100 µg protein conjugate per quarter of the labeled dose: Equity® Oestrous Control Vaccine, Zoetis, Australia). (auburn.edu)
This study demonstrates that heterologous prime-boost vaccination of mares using an Ad-GnRH prime and protein based GnRH vaccine boost results in an antibody response that suppresses reproductive cyclicity, and interferes with estrus behavior. (auburn.edu)
Vaccinated mares experience minimal side effects following vaccination with either Ad-GnRH or heterologous boost using a sub-effective dose (100µg protein conjugate) of a protein based GnRH vaccine. (auburn.edu)

Many clinically relevant tissues are refractory to Ad5 transduction because of negligible levels of the primary Ad5 receptor, the coxsackie and adenovirus receptor (CAR). (cnrs.fr)

Simply, the E1 gene products are early proteins that are transcribed in the early transcribed regions and required for proceeding subsequent steps in viral replication. (9lb.cc)

CAR receptor-fiber-knob adenovirus interaction and internalization process [46]. (genemedi.com)

Conserved E1B-55K SUMOylation in different human adenovirus species is a potent regulator of intracellular localization. (leibniz-liv.de)
Animal Models in Human Adenovirus Research. (leibniz-liv.de)
KIR3DS1 directs NK cell-mediated protection against human adenovirus infections. (leibniz-liv.de)
Adenovirus (AdV) is a member of the family Adenoviridae, whose name derives from their initial isolation from human adenoids in 1953 [42]. (genemedi.com)
Adenoviruses were first isolated in human adenoids (tonsils) from which its name is derived. (biologydiscussion.com)
In addition, a few types of human adenoviruses induce undifferentiated sarcomas in newborn hamsters and other rodents and can transform certain rodent and human cell cultures. (biologydiscussion.com)
Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy. (docksci.com)
The Human Papillomavirus (HPV) E2 protein, which inhibits the E6 and E7 viral oncogenes, is believed to have antioncogenic properties. (docksci.com)

Adenoviruses, in their natural state, are able to replicate in the body and lead to illnesses, such as the common cold. (medicalnewstoday.com)
In the case that the adenovirus is able to replicate, the likely side effects are a mild common cold for most people. (medicalnewstoday.com)

The AAV virion contains 60 VP proteins in a 1:1:10 ratio (VP1:VP2:VP3). (biomedcentral.com)
A couple of around 15 virally encoded protein that take part in the set up from the amorphous tegument framework, and these tegument protein occupy a lot of the mass in the virion (18, 25, 40). (thebiotechdictionary.com)
virion consists of dsDNA and protein. (biologydiscussion.com)

Hexon may be the many abundant adenoviral structural proteins, and studies also show that it's the major focus on for NAb (23, MCI-225 31, 32). (synanet2020.com)
This inhibitory effect has been associated with two viral non-structural proteins, NS3A and N^pro. (usda.gov)
Cleavage of structural proteins during the assembly of the head of bacteriophage T4. (microbiologyresearch.org)

This is an Adenovirus expressing shRNA for silencing of Mouse Atg7. (vectorbiolabs.com)
Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need. (9lb.cc)

We have constructed a nondefective recombinant virus between the nononcogenic adenovirus 5 (Ad5) and the highly oncogenic Ad12. (uu.nl)
This work pinpoints E2 as a key protein in the initiation of HPV-induced cervical cancer and identiﬁes the SAC as a target for oncogenic pathogens. (docksci.com)

Evidence that the Adenovirus Single-Stranded DNA Binding Protein Mediates the Assembly of Biomolecular Condensates to Form Viral Replication Compartments. (leibniz-liv.de)

Overexpression of VEGF protein in the lung at Days 1 to 10 was confirmed by enzyme-linked immunosorbent assay. (nih.gov)
Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. (vectorbiolabs.com)
Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3 (By similarity). (vectorbiolabs.com)
The BCKD enzyme is a multimeric enzyme complex with four components, branched-chain keto acid decarboxylase alpha and beta subunits (E1 α and E1 β ), dihydrolipoyl transacylase (E2) subunit, and dihydrolipoamide dehydrogenase (E3) subunit. (nature.com)

This shed light on the viral and cellular proteins that are involved in adenovirus replication. (scienceexhibitions.org)
Sonic 125256-00-0125B11 hedgehog (Shh) is a forty five-kDa potent signaling protein that regulates the proliferation, differentiation, and cellular patterning throughout a extensive selection of cell types [2,3]. (acthreceptor.com)

E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters. (9lb.cc)

Several adenoviruses cause respiratory and conjunctival diseases such as pneumonia, acute follicular conjunctivitis, epidemic keratoconjunctivitis, cystitis and gastroenteritis. (biologydiscussion.com)

This phenotype is associated with interaction of E2 with the Mitotic Checkpoint Complex (MCC) proteins Cdc20, MAD2 and BUBR1. (docksci.com)

Growth/differentiation factor-5 (GDF-5) belongs to the bone morphogenetic protein (BMP) family, which is expressed in dental pulp tissues. (ispub.com)
Growth/differentiation factor-5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family, which is a subgroup of the transforming growth factor- (TGF-) superfamily. (ispub.com)

The intravenous administration of adenovirus expressing sFlt-1, the secreted form of the extracellular website of the Flt-1 VEGF receptor, inside a collagen-induced arthritis (CIA) model results in obstructing of VEGF receptor signaling and a reduction in joint swelling [7]. (demotivate.org)

Adenovirus transcription is a two-phase event, early and late, occurring before and after viral DNA replication, respectively (Figure. (genemedi.com)
Life cycle of adenovirus is separated by DNA replication process into two distinct phases: the early and late, occurring before and after viral DNA replication, respectively. (genemedi.com)

Anatomy16

Organisms29

Diseases11

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes67

Information Science3

Vectors22

Serotype3

Genome9

Vector25

Genes5

Adeno-assoc1

Oncolytic adenovirus2

Vaccines3

Recombinant adenoviruses2

Infection12

Titer3

Tumor5

Promoter7

Transgene1

Virions2

Encodes2

Cells23

Capsid3

Particles2

Infect1

Type8

RAAV1

Antibodies4

Herpes1

Phylogenetic analysis1

Humans2

Replication-competent1

Vitro1

Vaccination5

Coxsackie1

Gene products1

Internalization1

Human8

Replicate2

Virion3

Structural3

ShRNA2

Oncogenic2

Mediates1

Enzyme4

Cellular2

Promoting transcription1

Respiratory1

Interaction1

Differentiation2

VEGF1

Early2