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  • Biol
  • The action of parathyroid hormone on the excretion and synthesis of lysosomal enzymes and on the extracellular release of acid by bone cells, J. Cell Biol. (springer.com)
  • proteins
  • A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. (uniprot.org)
  • Attachment to the bone matrix is facilitated by integrin receptors, such as αvβ3, via the specific amino acid motif Arg-Gly-Asp in bone matrix proteins, such as osteopontin. (wikipedia.org)
  • Species
  • PAPs are highly conserved within eukaryotic species, with >80% amino acid homology in mammalian PAPs, and >70% sequence homology in PAPs of plant origin. (wikipedia.org)
  • activity
  • When bone resorption was stimulated in vitro with either parathyroid hormone or 1,25(OH) 2 D 3 , there was a significant increase in both tartrate-resistant and tartrate-sensitive acid phosphatase activity in the medium relative to cultured controls. (springer.com)
  • Before mitosis (48 to 72 hours), a sharp increase in acid phosphatase activity occurs in cells stimulated with phytohemagglutinin. (sciencemag.org)
  • Samples were fixed at intervals after separation and incubated to demonstrate acid phosphatase activity. (rupress.org)
  • Some plant roots, especially cluster roots, exude carboxylates that perform acid phosphatase activity, helping to mobilise phosphorus in nutrient-deficient soils. (wikipedia.org)
  • Chief cells in parathyroid adenomas also display acid phosphatase activity. (wikipedia.org)
  • ExoIII activity depends partially on the DNA helical structure and displays sequence dependence (C>A=T>G). ExoIII has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities. (wikipedia.org)
  • Drawing on earlier research that indicated the presence of phosphoglycolic acid in algae through labeling of C14O2 and P28-orthophosphate, Richardson & Tolbert were the first to find a phosphatase activity specific for phosphoglycolate in tobacco leaves. (wikipedia.org)
  • In addition, Ca2+, despite being divalent, inhibits phosphoglycolate phosphatase on levels of greater than 90% of its enzymatic activity by acting as a competitive inhibitor to Mg2+. (wikipedia.org)
  • localization
  • Tartrate-resistant acid phosphatases of bone may be suitable biochemical probes for osteoclast function, but it will be necessary to achieve further purification in order to develop analytical methods with sufficient sensitivity and specificity (e.g., immunochemical) to ensure precise localization and quantitation. (springer.com)
  • catalyzes
  • Phosphoglycolate, which is a potent inhibitor of phosphofructokinase and triosephosphate isomerase, must be quickly metabolized and transformed into a useful substrate, and phosphoglycolate phosphatase catalyzes the first step in the regeneration of 3-phosphoglycerate from 2-phosphoglycolate at the expense of energy in the form of ATP. (wikipedia.org)
  • Osteoclast
  • Organ cultures of newborn mouse calvaria were used to test the hypothesis that tartrate-resistant acid phosphatase might serve as a biochemical marker for osteoclast function. (springer.com)
  • The osteoclast disassembles and digests the composite of hydrated protein and mineral at a molecular level by secreting acid and a collagenase, a process known as bone resorption. (wikipedia.org)
  • osteoclasts
  • The results obtained from biochemical assays, histochemical observations, and polyacrylamide gel electrophoresis suggest that bone resorption in vitro results in the release of tartrate-resistant acid phosphatase from osteoclasts and tartrate-sensitive acid phosphatase from other bone cells as well as osteoclasts. (springer.com)
  • histidine
  • Belongs to the histidine acid phosphatase family. (abcam.com)
  • This is evidence that the active site requires this "claw" to hold on to the phosphate group, the aspartic acid residue to activate a water molecule, and the histidine residue to provide a proton to form the alcohol. (wikipedia.org)