• PBM-treated hypertrophic cells (1064 nm and 17.6 J/cm2 every day for 7 days following addition of PA) decreased the lipid levels in hypertrophic adipocytes, restored the GLUT4 protein expression and enhanced glucose transport. (google.com)
  • Adipocytes were recently identified as an important source of endocrine and paracrine mediators, regulating the metabolism and activity of various cell types and body functions. (pensoft.net)
  • We investigated the role of S100B in the interaction between adipocytes and macrophages using a cell-culture model. (nih.gov)
  • 3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. (nih.gov)
  • KSS inhibited the accumulation of triglycerides in a dose-dependent manner in 3T3-L1 cells that were induced to differentiate into adipocytes. (elsevierpure.com)
  • The differentiation of precursor cells into mature adipocytes (adipogenesis) has been an area of increased focus, spurred by a rise in obesity rates. (oregonstate.edu)
  • Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes. (rndsystems.com)
  • TLK16998 alone had no effect on IR signaling in mouse 3T3-L1 adipocytes but, at concentrations as low as 3.2 μmol/l, enhanced the effects of insulin on the phosphorylation of the IR β-subunit and IR substrate 1, and on the amount of phosphatidylinositol 3-kinase that coimmunoprecipitated with IRS-1. (diabetesjournals.org)
  • TLK16998 also increased the potency of insulin in stimulating 2-deoxy- d -glucose uptake in 3T3-L1 adipocytes, with a detectable effect at 8 μmol/l and a 10-fold increase at 40 μmol/l. (diabetesjournals.org)
  • MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. (sigmaaldrich.com)
  • In IR adipocytes, uptake of D3 and 25-hydroxyvitamin-D3 was higher, but, after adrenaline stimulation, the decrement in D3 and 25-hydroxyvitamin-D3 was stronger in control cells, which also showed increased expression of Cyp27a1 and Cyp27b1 and higher levels of 25-hydroxyvitamin-D3. (medscape.com)
  • Pimozide, a novel fatty acid binding protein 4 inhibitor, promotes adipogenesis of 3T3-L1 cells by activating PPARγ. (ox.ac.uk)
  • [ 16 ] Potential mechanisms through which TCF7L2 variants influence T2DM include its role in adipogenesis, myogenesis, and pancreatic islet development, as well as in beta-cell survival and insulin secretory granule function. (medscape.com)
  • [ 17 , 18 ] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. (medscape.com)
  • Finally, lipid accumulation in cells induced to undergo adipogenesis and treated with BPC decreased although without significant differences at different concentrations. (unav.edu)
  • 2017. Cysteine deprivation prevents induction of peroxisome proliferator-activated receptor gamma-2 and adipose differentiation of 3T3-L1 cells. (vkm.no)
  • Organelle relationships in cultured 3T3-L1 preadipocytes. (silverchair.com)
  • The morphological differentiation of 3T3-L1 preadipocytes includes the loss of "stress fibers" and the appearance of microfilament like structures that encase, in a complex manner, the cytosolic lipid spheres that appear during differentiation. (silverchair.com)
  • 3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. (pensoft.net)
  • Here, we demonstrate the molecular mechanism underlying the effect of KSS on the differentiation of mouse preadipocytes (3T3-L1 cells). (elsevierpure.com)
  • In the 3T3-L1 cells HuR is constitutively expressed and localized predominantly to the nucleus in the preadipocytes. (uncg.edu)
  • Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. (foodandnutritionresearch.net)
  • Discussion : Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. (foodandnutritionresearch.net)
  • Ilieva B, Marinova E, Gagov H, Konakchieva R (2017) Basal and PGF 2α -stimulated secretion of pro-inflammatory cytokines from 3T3-L1 adipocyte-like cells. (pensoft.net)
  • Differentiation of 3T3-L1 murine fibroblasts into adipocyte-like cells was conducted in presence of IBMX, dexamethasone and insulin and demonstrated by Oil Red O staining of accumulated lipid droplets. (pensoft.net)
  • It is concluded that pro-inflammatory phenotype of differentiated 3T3-L1 adipocyte-like cells, induced by PGF 2α is characterized by enhanced TNF α production which critically depends on the ability of CSE to produce H 2 S. (pensoft.net)
  • Using a mouse model of genetic copper misregulation, in combination with pharmacological alterations in copper status and imaging studies in a 3T3-L1 white adipocyte model, we found that copper regulates lipolysis at the level of the second messenger, cyclic AMP (cAMP), by altering the activity of the cAMP-degrading phosphodiesterase PDE3B. (nih.gov)
  • Concentrations of cholesterol and triglycerides are strongly correlated in the adipocyte, but little is known about mechanisms regulating cholesterol metabolism in fat cells. (jci.org)
  • the effects of hypoxia and fibrosis may also play a role in obesity complications via notably ectopic-lipid accumulation in non-adipocyte cells. (springer.com)
  • Alteration by 2,3,7,8-Tetrachlorodibenzo-p-dioxin of CCAAT/enhancer binding protein correlates with suppression of adipocyte differentiation in 3T3-L1 cells. (aspetjournals.org)
  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds elicit multiple effects on the function of adipose tissue and adipogenic cell lines, including the suppression of adipocyte differentiation. (aspetjournals.org)
  • Gel shift analysis of nuclear extract binding to an oligonucleotide containing a C/EBP DNA recognition sequence revealed no difference between extracts from control and TCDD-treated cells in the binding pattern at day 2 of differentiation. (aspetjournals.org)
  • Ethanolic extracts were used in 3T3-L1 cell culture. (unav.edu)
  • S100B upregulated TNF-α and M1 markers in RAW, and TNF-α augmented S100B secretion from L1. (nih.gov)
  • L1 conditioned media stimulated TNF-α secretion from RAW, and RAW conditioned media increased S100B secretion from L1. (nih.gov)
  • Finally, TCF7L2 polymorphisms have been associated with impaired insulin secretion, glucose production, and glucose tolerance via direct effects on pancreatic islet beta cells. (medscape.com)
  • Before starting any insulin resistance-inducing treatment, cells were washed with PBS and changed to serum-free, low-glucose (1g/L) DMEM with 0.5% BSA. (nih.gov)
  • In a twist, there are let-7-binding sites in MYC 3' untranslated region(UTR) according to bioinformatic analysis, and let-7 overexpression in cell culture decreased MYC mRNA levels. (wikipedia.org)
  • In differentiating 3T3-L1 cells, lipid spheres, the endoplasmic reticulum (ER), microperoxisomes, and mitochondria form "constellations" that may reflect the interplay of lipid metabolizing enzymes in these organelles. (silverchair.com)
  • At higher doses, cells undergo mitochondrial destabilization due to calcium release from the endoplasmic reticulum, and die. (hindawi.com)
  • The mutants of other let-7 family members have a radio-resistant phenotype in vulval cells, which may be related to their ability to repress RAS. (wikipedia.org)
  • Two days after confluence (Day 0), cells were induced to differentiate with DMEM containing 10% fetal bovine serum (FBS) , 1µM dexamethasone, 10 µg/ml insulin and 0.5mM 3-isobutyl-1-methyxanthine for 2 days. (nih.gov)
  • Cells were then incubated in DMEM containing 10% FBS and 10 µg/ml insulin for 2 more days. (nih.gov)
  • NF-κB pathways are associated with immunity, inflammation and cell survival. (springer.com)
  • MAPK pathways refer to various cellular functions, including cell proliferation, differentiation and migration. (springer.com)
  • Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
  • However, lipid accumulation in non-specialized cells is deleterious and can contribute to cell death resulting in the development of pathology such as NASH (Non-Alcoholic Steatohepatitis) a liver injury associated with obesity [ 12 ]. (springer.com)
  • However, the diseased state of these cells may impede in vitro fibrocartilage formation. (oregonstate.edu)
  • In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
  • TP53 is a tumor suppressor gene that functions in modifying DNA errors and/or inducing apoptosis of damaged cells, and both APC and BECN1 genes are involved in CRC and are of importance in cellular growth and metastasis. (scielo.br)
  • and negative regulation of cell population proliferation. (nih.gov)
  • These data indicate that suppression of differentiation in the 3T3-L1 preadipocyte cell line by TCDD occurs at a short but defined period, during the differentiation program, and involves altered regulation of C/EBP, including the inhibition of C/EBP alpha. (aspetjournals.org)
  • Prostaglandin receptor EP3 regulates cell proliferation and migration with impact on survival of endometrial cancer patients. (rndsystems.com)
  • Thus any modification of ECM components can be transmitted via integrins and impact on cell movement during development and proliferation, differentiation, apoptosis. (springer.com)
  • As in hepatocytes and absorptive cells of the intestine, this spatial relationship of ER and plasmalemma suggests a role for rosettes in the uptake of exogenous lipid precursors. (silverchair.com)
  • Although the levels of mature let-7 members are undetectable in undifferentiated cells, the primary transcripts and the hairpin precursors of let-7 are present in these cells. (wikipedia.org)
  • Objective : We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. (foodandnutritionresearch.net)
  • In the same experimental system, IL-10 and BDNF gene expressions increased, and in HypoE22 cells, the extract decreased the extracellular dopamine level and increased the DAT gene expression due to the direct interaction of parthenolide with the DAT. (researchgate.net)
  • Design : 3T3-L1 cells were differentiated in the presence of different concentrations of TME. (foodandnutritionresearch.net)
  • Cancer cell viability was analyzed using MTT assay, and the results showed a significant increase in the number of viable cells after 24 h of treatment with MSG with different concentrations (0.5, 1.0, 10, 50, and 100mM). (scielo.br)
  • These potent effects are driven by curcumin's ability to induce G2/M cell cycle arrest, induce autophagy, activate apoptosis, disrupt molecular signaling, inhibit invasion and metastasis, and increase the efficacy of current chemotherapeutics. (hindawi.com)
  • Current research is focused on the mechanisms that regulate SMAD activity to evoke cell-type-specific and context-dependent transcriptional programmes. (nature.com)
  • Immunoblot analysis with specific antibodies to each C/EBP isoform demonstrated that the levels of C/EBP delta and C/EBP beta protein were rapidly induced (by day 1) and then abrogated by day 4 and 8, respectively, in solvent-treated (control) cells. (aspetjournals.org)
  • In TCDD-treated cells, however, the levels of C/EBP beta and C/EBP delta protein persisted at these time points. (aspetjournals.org)
  • in both IR cells and obese SAT, protein expression of β 2 -adrenergic receptor was reduced. (medscape.com)
  • Curcumin causes cell death and primarily initiates an autophagic step (mitophagy). (hindawi.com)
  • Granéli C, Karlsson C, Brisby H, Lindahl A and Thomsen P: The effects of PPAR-γ inhibition on gene expression and the progression of induced osteogenic differentiation of human mesenchymal stem cells. (spandidos-publications.com)
  • The effects on dopamine (DA) release and dopamine transporter (DAT) gene expression were assayed in hypothalamic HypoE22 cells. (researchgate.net)
  • We began to examine the mechanism by which TCDD inhibits differentiation of the established preadipocyte cell line 3T3-L1. (aspetjournals.org)
  • Given that the expression levels of let-7 members are significantly low in human cancers and cancer stem cells, the major function of let-7 genes may be to promote terminal differentiation in development and tumor suppression. (wikipedia.org)
  • As one of the genes involved in (but not essential for) induced pluripotent stem (iPS) cell reprogramming, LIN28 expression is reciprocal to that of mature let-7. (wikipedia.org)
  • In the present study, the effect of MSG on cell viability and its effect on expression of APC , BECN1 , and TP53 genes in SW620 and SW480 colon cancer cell lines were studied. (scielo.br)
  • Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. (sigmaaldrich.com)
  • WB: SW480 whole cell lysate ( ab3957 ), SW40, HeLa and SH-SY5Y cell lysates. (abcam.com)
  • The co-culture of RAW and L1 increased TNF-α, S100B, and the expression of M1 markers and the MCP-1 receptor CCR2. (nih.gov)
  • conversely, TP53 showed a significant decrease in expression in SW620 cells. (scielo.br)
  • For hypoxia treatments, cells were incubated in a 1% oxygen chamber (Powers, Millman et al. (nih.gov)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • RESEARCH particular, timing of return to roost, leaving for feeding, monolayer of Vero cells. (cdc.gov)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • His current research is on wound healing, anti-cancer, anti-obesity and anti-diabetes study of several medicinal plants using animal cell culture. (iium.edu.my)
  • Frequently, low doses of natural chemical products activate an adaptive stress response, whereas high doses activate acute responses like autophagy and cell death. (hindawi.com)
  • Cell Death Differ, 2023 May. (nih.gov)
  • Taken together, PBM is shown capable of restoring the cellular morphology and function of hypertrophic cells. (google.com)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Use of autologous synoviocytes from the affected stifle is an attractive cell source for tissue engineering replacement fibrocartilage. (oregonstate.edu)