• differentiation process
  • Duplicate RNA samples were collected daily during the differentiation process and probed with the Affymetrix U74Av2 GeneChip® microarray to allow the time-course analysis of the gene expression profile in these differentiating cells. (ualberta.ca)
  • mRNA
  • The levels of mRNA are shown as ratios relative to GST treated cells. (nih.gov)
  • The level of IL-6 mRNA expression was significantly increased in 3T3-L1 cells treated with 70Q for 24 h compared with wild HCV core protein (Fig 1A). (nih.gov)
  • On the other hand, 70Q induced significant reduce in mRNA expressions of adiponectin and leptin compared to control (GST), though the differences between 70R and 70Q-treated cells did not reach statistical significance due to wide variation of data range (Fig 1B and 1C). (nih.gov)
  • Similar to the changes in mRNA expression, the protein level of IL-6 was significantly increased in 70Q cells (Fig 2A), and the levels of adiponectin and leptin were significantly reduced in 70Q cells (Fig 2B and 2C). (nih.gov)
  • Hexane-soluble fraction of the root extract also inhibited adipocyte differentiation and decreased the mRNA levels of various adipogenic genes in the differentiating cells. (biomedcentral.com)
  • In humans, chemerin mRNA is highly expressed in white adipose tissue, liver and lung while its receptor, CMKLR1 is predominantly expressed in immune cells as well as adipose tissue. (wikipedia.org)
  • Mice
  • There was also a GPR84 downregulation in dentritic cell derived from FcRgamma chain KO mice. (wikipedia.org)
  • Ablating lysosomal acid lipase (Lal-/-) in mice led to aberrant expansion of myeloid-derived suppressive cells (MDSCs) (>40% in the blood, and >70% in the bone marrow) that arise from dysregulated production of myeloid progenitor cells in the bone marrow. (wikipedia.org)
  • Ly6G + MDSCs in Lal-/- mice show strong immunosuppression on T cells, which contributes to impaired T cell proliferation and function in vivo. (wikipedia.org)
  • glucose
  • Glucose is an important source of energy for mammalian cells and enters the cytosol via glucose transporters. (mdpi.com)
  • Here, we used the fluorescent indicator protein (FLIPglu-600µ) to monitor cytosolic glucose dynamics in mouse 3T3-L1 cells in which glucose utilization for glycogen synthesis was inhibited. (mdpi.com)
  • The results show that cells exhibit a low resting cytosolic glucose concentration. (mdpi.com)
  • However, in cells with inhibited glycogen synthase activation, insulin induced a robust increase in cytosolic free glucose. (mdpi.com)
  • The insulin-induced increase in cytosolic glucose in these cells is due to an imbalance between the glucose transported into the cytosol and the use of glucose in the cytosol. (mdpi.com)
  • In untreated cells with sensitive glycogen synthase activation, insulin stimulation did not result in a change in the cytosolic glucose level. (mdpi.com)
  • This is the first report of dynamic measurements of cytosolic glucose levels in cells devoid of the glycogen synthesis pathway. (mdpi.com)
  • At the cellular level, sortilin functions in protein transport between the Golgi apparatus, endosome, lysosome, and plasma membrane, leading to its involvement in multiple biological processes such as glucose and lipid metabolism as well as neural development and cell death. (wikipedia.org)
  • proliferation
  • The proliferation rate of the cells was measured and compared by a non-isotope method-XTT method. (koreascience.or.kr)
  • CEBPD is involved in regulation of apoptosis and cell proliferation. (wikipedia.org)
  • Subsequent studies revealed that the dye can be used to monitor lymphocyte proliferation, both in vitro and in vivo, due to the progressive halving of CFSE fluorescence within daughter cells following each cell division. (wikipedia.org)
  • By the use of fluorescent antibodies against different lymphocyte cell surface markers it is also possible to follow the proliferation behaviour of different lymphocyte subsets. (wikipedia.org)
  • Furthermore, the use of CFSE has extended beyond the immune system, with the dye being used to monitor the proliferation of many other cell types such as smooth muscle cells, fibroblasts, hematopoietic stem cells and even bacteria. (wikipedia.org)
  • expression
  • Gene Expression during 3T3-L1 Cell. (ualberta.ca)
  • Recently it has been shown that epigenetic modification of the distal promoter region of CEBPA has resulted in downregulation of CEBPA expression in pancreatic cancer cells, lung cancer, and head and neck squamous cell carcinoma. (wikipedia.org)
  • C/EBPβ plays a role in neuronal differentiation, in learning, in memory processes, in glial and neuronal cell functions, and in neurotrophic factor expression. (wikipedia.org)
  • 1,25-(OH)2D3 transiently but strongly induces Insig-2 expression in 3T3-L1 cells. (wikipedia.org)
  • extract
  • A processing problem is difficult to extract the intercellular useful component, which is the most problematic solid body, and cell wall filling substance, which is the most problem in the processing of seaweeds. (omicsonline.org)
  • patterns
  • The high stability and reproducibility of the 3T3-L1 gene transcription patterns over different experiments and cell batches is demonstrated by this study. (uantwerpen.be)
  • inhibition
  • The effects of toll-like receptor (TLR)2/4 inhibition on IL-6 production by 3T3-L1 induced by HCV core protein were examined. (nih.gov)
  • arrest
  • Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. (wikipedia.org)
  • genes
  • Genes coding for transporter proteins that confer multidrug resistance to the cells have also been found to be activated by CEBPB. (wikipedia.org)
  • protein levels
  • A) The protein level of IL-6 was significantly increased, and the protein levels of (B) adiponectin and (C) leptin were significantly reduced in cells treated with 70Q for 48 h compared with 70R and GST protein. (nih.gov)
  • compound
  • Therefore, we demonstrated that FA is a positive regulator of HO-1 in 3T3-L1, and may be an effective bioactive compound to reduce adipocyte tissue mass. (mdpi.com)
  • skeletal muscle cells
  • This molecular function enables sortilin to participate in various biological processes, including the transport of GLUT4 to the plasma membrane of fat and skeletal muscle cells in response to insulin. (wikipedia.org)
  • Interestingly
  • Interestingly, a significant role for sortilin has recently also been reported in the field of oncology, as it has been detected in several cancer cell lines. (wikipedia.org)
  • effects
  • Here, the tissue-specific effects of capsaicin and nonivamide on parameters of mitochondrial energy metabolism in 3T3-L1 and HepG2 cells are investigated. (rsc.org)
  • Retinoids exert biologic effects such as potent growth inhibitory and cell differentiation activities and are used in the treatment of hyperproliferative dermatological diseases. (wikipedia.org)
  • receptor
  • As a sorting receptor on the cell surface and on the endoplasmic reticulum-Golgi apparatus within the cell, sortilin is involved in the transport of a wide variety of intracellular proteins between the trans-Golgi network, endosome, lysosome, and secretory granules, as well as the plasma membrane. (wikipedia.org)
  • It also mediates the interaction between proNGF and the p75NTR:sortilin complex by acting as a co-receptor to signal cell death. (wikipedia.org)
  • RARRES2 is thought to act as a cell surface receptor. (wikipedia.org)
  • myeloid
  • GPR84 is normally expressed at low levels in myeloid cells and can be induced in vitro by stimulating macrophage or microglial cells with LPS, TNFα, or PMA. (wikipedia.org)
  • neuronal
  • The fine regulation of the brain-derived neurotrophic factor (BDNF) by sortilin is required for both neuronal and tumor cell survival. (wikipedia.org)
  • mammalian
  • This protein is expressed in the mammalian nervous system and plays a significant role in the development and function of nerve cells. (wikipedia.org)