Loading...
  • fluorescence
  • It is found that looped-out 2-AP, an analogue of adenine, in split parallel G-quadruplexes, triplexes and duplexes always shows much higher fluorescence intensity than that in single- or double-stranded DNAs, due to the weaker quenching effects derived from the reduced base stacking environments. (rsc.org)
  • Taking advantage of these traits, we introduce a new strategy to monitor the behaviours of DNA nanodevices via the fluorescence signal output by utilizing changes in the base stacking environment of 2-AP. (rsc.org)
  • Compared with conventional fluorescence labelling, looped-out 2-AP as a reporter shows good photostability and can be quenched by base-pairing, thereby providing an efficient quencher-free methodology for monitoring DNA nanodevices. (rsc.org)
  • This approach permitted us to discriminate between the local conformation of the unpaired adenine in several RNA states during dimerization dimerization: hairpin, a kissing loop dimer (KD) and an extended duplex dimer (ED). It was shown that fluorescence intensity of 2-AP in the monomer hairpin RNA has higher than that of the both RNA dimers. (jbsdonline.com)
  • It has also been found that the intensity of 2-AP fluorescence for the two RNA dimers is different. (jbsdonline.com)
  • We studied the conformational change of the unpaired loop adenine of kissing loop dimer RNA upon the interaction with aminoglycoside antibiotics (AMG): paromomycin, neomycin, tobramycin and kanamycin B. It turned out that only tobramycin increases nearly three times the intensity of fluorescence 2-AP of kissing loop dimer. (jbsdonline.com)
  • This result implies that 2-AP loses the initial intra-loop interactions in the structure KD on interaction with tobramycin, becoming more exposed into the solution that is reflected in its fluorescence increase. (jbsdonline.com)
  • Probably, the fluorescence of 2-aminopurine can be successfully used to detect the binding of ligands to different structures of RNA. (jbsdonline.com)
  • purine
  • 2-Aminopurine, a purine analog of guanine and adenine, is a fluorescent molecular marker used in nucleic acid research. (wikipedia.org)
  • A cyclopropane derivative of formula (I) ##STR1## wherein B1 is a purine or pyrimidine residue, R1 and R2 are, independently, hydrogen or a protecting group for hydroxyl and each of k, m and n represents, independently, an integer of 1 or 2 is useful for its antiviral effect. (google.com)
  • Antiviral Carbocyclic Purine nucleosides (-)Carbovir Abacavir Entecavir Carbocyclic 2′-ara-fluoro-guanosine Entecavir, a guanosine analog, was reported in 1997 as a potent and selective inhibitor for the hepatitis B virus, and approved by the FDA in March 2005 for oral treatment of hepatitis B infection. (wikipedia.org)
  • base
  • Linear approaches to chiral carbocyclic nucleosides 2 rely on the construction of the heterocyclic base onto a suitable protected chiral cyclopentylamine (1 → 2). (wikipedia.org)
  • In the convergent approach the intact heterocyclic base is coupled directly to a suitably protected functionalised carbocyclic moiety (3 → 2). (wikipedia.org)
  • 5-Bromouracil, systematic name 5-bromopyrimidine-2,4-dione, (abbreviated as 5-BrU, 5BrUra or br5Ura) is a brominated derivative of uracil that acts as an antimetabolite or base analog, substituting for thymine in DNA, and can induce DNA mutation in the same way as 2-aminopurine. (wikipedia.org)
  • Herpes
  • Carbocyclic (E)-5-(2-bromovinyl)-2-deoxyuridine( (+) C-BVDU) GR95168 possesses activity against herpes simplex virus type l (HSV-1) and varicella zoster virus (VZV, chicken pox and shingles) in-vitro and in-vivo. (wikipedia.org)
  • potential
  • 2-Aminopurine inhibits lipid accumulation induced by apolipoprotein E-deficient lipoprotein in macrophages: potential role of eukaryotic initiation factor-2 α phosphorylation in foam cell formation," Journal of Pharmacology and Experimental Therapeutics , vol. 326, no. 2, pp. 395-405, 2008. (hindawi.com)
  • journal
  • Apolipoprotein E-deficient lipoproteins induce foam cell formation by activation of PERK-EIF-2 α signaling cascade," Journal of Bioanalysis and Biomedicine , vol. 2, no. 5, pp. 113-120, 2010. (hindawi.com)
  • J. Lewerenz and P. Maher, "Basal levels of eIF2 α phosphorylation determine cellular antioxidant status by regulating ATF4 and xCT expression," Journal of Biological Chemistry , vol. 284, no. 2, pp. 1106-1115, 2009. (hindawi.com)