*  Buoyant density ultracentrifugation
Buoyant density centrifugation uses the concept of buoyancy to separate molecules in solution. Usually a caesium chloride (CsCl) solution is used, but in the general case it's usually approximately the same density as the molecules that are to be centrifuged. The sample is put on top of the solution, and then the tube is spun at a very high speed for an extended time, at times lasting days. The CsCl molecules become densely packed toward the bottom, so even layers of different densities form. Since the original solution was approximately the same density, they go to a level where their density and the CsCl density are the same, to which they form a sharp, distinctive band. This method very sharply separates molecules, and is so sharp that it can even separate different molecular isotopes from one another. Buoyant density of majority of DNA is 1.7g/cm3 which is equal to density of 6M CsCl solution. Buoyant density of DNA changes with its GC content ...
*  Ultracentrifuge
... an Analytical Ultracentrifugation Forum) Analytical Ultracentrifugation as a Contemporary Biomolecular Research Tool. Multi- ... Analytical ultracentrifugation has recently seen a rise in use because of increased ease of analysis with modern computers and ... Modern Analytical Ultracentrifugation in Protein Science: A tutorial review Reversible Associations in Structural and Molecular ... Gas centrifuge Theodor Svedberg Differential centrifugation Buoyant density ultracentrifugation Zippe-type centrifuge "Optima ...
*  Association of Biomolecular Resource Facilities
Biophysics: calorimetry, CD, fluorescence, light scattering, SPR, ultracentrifugation. Flow Cytometry Fluorescence Activating ...
*  Centrifugation
Ultracentrifugation makes use of high centrifugal force for studying properties of biological particles. Compared to ... Howlett, G.J., Minton, A.P., Rivas, G. Analytical Ultracentrifugation for the Study of Protein Association and Assembly. ... Cole, J.L., Hansen, J.C. Analytical Ultracentrifugation as a Contemporary Biomolecular Research Tool. Methods and Reviews, 1999 ... Cao, W., Demeler B. Modeling Analytical Ultracentrifugation Experiments with an Adaptive Space-Time Finite Element Solution for ...
*  Exosome (vesicle)
Combining ultracentrifugation with micro-filtration or a gradient can improve purity. Single step isolation of extracellular ... Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, Simpson RJ (2012). "Comparison of ultracentrifugation, density ... Isolation of exosomes using differential ultracentrifugation results in co-isolation of protein and other contaminants and ...
*  High-density lipoprotein
Warnick GR, Nauck M, Rifai N (Sep 2001). "Evolution of methods for measurement of HDL-cholesterol: from ultracentrifugation to ... Since the HDL particles have a net negative charge and vary by density & size, ultracentrifugation combined with ... laboratories formerly measured HDL cholesterol by separating other lipoprotein fractions using either ultracentrifugation or ...
*  Julius Marmur
... density-gradient ultracentrifugation and by direct inspection using electron microscopy. Further evidence of reversible ...
*  Photophoresis
If developed the technique would be orders of magnitudes faster than currently established ultracentrifugation techniques. ...
*  Dynamic light scattering
The method is also used for the quantification of sedimentation velocity data from analytical ultracentrifugation. The maximum ...
*  List of Swedish inventors
Theodor Svedberg (1884-1971) invented the ultracentrifugation method for determination of molecular weights in 1924. Anders ...
*  Howard Schachman
1] Kresge, Nicole; Simoni, Robert D.; Hill, Robert L. (May 25, 2007). "Innovations in ultracentrifugation and an analysis of ...
*  Translin
Lee SP, Fuior E, Lewis MS, Han MK (Nov 2001). "Analytical ultracentrifugation studies of translin: analysis of protein-DNA ...
*  Diagnostic microbiology
With high speed buoyant density ultracentrifugation, a density gradient is created with caesium chloride in water. DNA will go ...
*  Wolfgang Patsch
Using zonal ultracentrifugation methods developed by Josef R. Patsch (and in collaboration with him), a detailed physical and ... Characterization of human high density lipoproteins by zonal ultracentrifugation. J Biol Chem. 1980;255: 3178-3185. Goldberg AP ... Isolation of subfractions of human very low density lipoproteins by zonal ultracentrifugation. J Biol Chem. 1978; 253:4911-4915 ...
*  Lamm equation
The Lamm equation describes the sedimentation and diffusion of a solute under ultracentrifugation in traditional sector-shaped ...
*  Lipoprotein
They are classified on the basis of electrophoresis, ultracentrifugation and Nuclear Magnetic Resonance spectroscopy via the ...
*  Immunoglobulin M
... studied by ultracentrifugation, electrophoresis, and electron microsopy". Scandinavian Journal of Immunology. 4 (3): 217-228. ...
*  Protein
... s may be purified from other cellular components using a variety of techniques such as ultracentrifugation, ... The resulting mixture can be purified using ultracentrifugation, which fractionates the various cellular components into ...
*  Sedimentation equilibrium
It forms the basis for an analytical ultracentrifugation method for measuring molecular masses, such as those of proteins, in ...
*  Ebola virus
The overall shape of the virions after purification and visualization (e.g., by ultracentrifugation and electron microscopy, ...
*  Staphylokinase
In solution the protein structure was analyzed by X-ray scattering, dynamic light scattering, ultracentrifugation and UV ...
*  Type three secretion system
After several rounds of lysis and washing, the opened bacteria are subjected to a series of ultracentrifugations. This ...
*  Theodor Svedberg
During this work, he developed the technique of analytical ultracentrifugation, and demonstrated its utility in distinguishing ...
*  Synthesis of carbon nanotubes
The most efficient method relies on density-gradient ultracentrifugation, which separates surfactant-wrapped nanotubes by the ...
*  Hyperlipidemia
... which is based on the pattern of lipoproteins on electrophoresis or ultracentrifugation. It was later adopted by the World ...