... found that brain tumor stem cells (BTSCs) are stem cells that can be isolated from brain tumors by similar assays used for ... While it remains to be definitely proven whether the SVZ stem cells are the cell of origin for brain tumors such as gliomas, ... In addition, some current theories propose that the SVZ may also serve as a site of proliferation for brain tumor stem cells ( ... neuronal stem cells.[5] In forming clonal spheres similar to neurospheres of neuronal stem cells, these BTSCs were able to ...
This in vivo assay is called a limiting dilution assay. The tumor cell subsets that can initiate tumor development at low cell ... "Critical appraisal of the side population assay in stem cell and cancer stem cell research". Cell stem cell. 8 (2): 136-47. doi ... a critical review of sphere-formation as an assay for stem cells". Cell stem cell. 8 (5): 486-98. doi:10.1016/j.stem.2011.04. ... They examined cancer stem cell plasticity in which cancer stem cells can transition between non-cancer stem cells (Non-CSC) and ...
Subsequently, PBMCs are challenged with tumor antigens such as tumor stem cell antigen. Inflammatory cytokines are usually ... PBMC fraction also contains progenitor populations, as demonstrated by methylcellulose based colony forming assays. PBMCs have ... is any peripheral blood cell having a round nucleus. These cells consist of lymphocytes (T cells, B cells, NK cells) and ... The polymorphonuclear cells can be further isolated by lysing the red blood cells. Basophils are sometimes found in both the ...
... cell types and is implicated in drug resistance and metastasis due to its ability to promote mesenchymal transition and stem ... Modern anti-tTG assays rely on a human recombinant protein as an antigen. It's still experimental to use tTG as a form of ... It is also being studied as an attenuator of metastasis in certain tumors. TG2 participates in both enzymatic and non-enzymatic ... International Review of Cell and Molecular Biology. 294: 1-97. doi:10.1016/B978-0-12-394305-7.00001-X. ISBN 9780123943057. PMC ...
Rat tumor cell lines GH3 (pituitary tumor) PC12 (pheochromocytoma) Plant cell lines Tobacco BY-2 cells (kept as cell suspension ... Culture of human stem cells is used to expand the number of cells and differentiate the cells into various somatic cell types ... immortality Cell culture assays Electric cell-substrate impedance sensing List of contaminated cell lines List of NCI-60 Cell ... cells Cell-to-cell contact can stimulate cell cycle arrest, causing cells to stop dividing, known as contact inhibition. Cell- ...
Tumor Stem Cell Laboratory Models - CERN Foundation laboratory scientists seek to understand how normal neural development goes ... This project seeks to improve the accuracy in the diagnosis of ependymoma and develop the necessary tools to create new assays ... Tumor Profiling and Pathology - An integrated histology and molecular biology grading system is used to improve the accuracy of ... "Cancer Cell", September 2011. Establishment and characterization of clinically relevant models of ependymoma: a true challenge ...
In 1980-82 his group first identified (and provided an in vitro assay for) the human mesenchymal stem cell - the precursor of ... progenitor cell was identified and shown to play an important role in the development of the blood supply of developing tumors ... Such stem cell transplants give a faster recovery of white blood cells, platelets and red cells than seen with conventional ... the cancer stem cell in lung cancer and ovarian cancer and has developed some novel methods for growing human cancer stem cells ...
... against tumor cell surface glycoproteins to identify breast tumour cells in bone marrow and peripheral blood. They concluded ... In order to effectively stage breast cancer and assess the efficacy of purging regimens prior to autologous stem cell infusion ... performed a sensitive immuno-cytochemical assay by using a combination of four monoclonal antibodies (260F9, 520C9, 317G5 and ... These isolated B cells are in turn fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a ...
... directing migration of human hematopoietic stem cells and mesenchymal stem cells to bone marrow.[11] Ex vivo glycan engineering ... Konstantopoulos K, Thomas SN (2009). "Cancer cells in transit: the vascular interactions of tumor cells". Annual Review of ... CD44 expression is an indicative marker for effector-memory T-cells. Memory cell proliferation (activation) can also be assayed ... The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. In humans, the ...
Cancer Stem Cells from Human Breast Tumors are Involved in Spontaneous Metastases in Orthotopic Mouse Models. Proceedings of ... His research focuses on the development of imaging assays to monitor fundamental cellular/molecular events in living subjects ... 2016, early ed.). Molecular profiling of single circulating tumor cells from lung cancer patients. PNAS. Weissleder R, ... "Intracellular Aggregation of Multimodal Silica Nanoparticles for Ultrasound-Guided Stem Cell Implantation". Science ...
Frequently used assays include the ELISPOT, which measures the frequency of T cell response. It is similar to the ELISA assay ... Another method is hematopoietic stem-cell transfer, used for studying lymphocyte development. Hematopoietic cells are killed by ... 2009). "Adoptive transfer of virus-specific and tumor-specific T cell immunity." Current Opinion in Immunology 21(2): 224-232. ... Cells of interest are bound to antibody-coated plastic surfaces, and unwanted cells are removed by treatment with specific ...
The underlying tumors are almost exclusively of B-cell lineage. However, T-cells and CD56+ Natural Killer cells have also been ... This is followed by transplanting peripheral blood stem cells. If the lesions are mild, the patient will be subject to a good ... Further research in 2013 outlined the various types of assays that could be used to determine which antibodies were involved in ... It is more hopeful if the tumor is benign, but in the case of malignant tumors, the mortality rate is roughly 90%. The two most ...
Functional assays of NKX3-1 mutant mice in serial prostate regeneration suggested that NKX3-1 is required for stem cell ... "Stabilization of the prostate-specific tumor suppressor NKX3.1 by the oncogenic protein kinase Pim-1 in prostate cancer cells ... Single-cell transplantation assays showed that castration-resistant NKX3-1 expressing cells (CARNs) can reconstitute prostate ... "A luminal epithelial stem cell that is a cell of origin for prostate cancer". Nature. 461 (7263): 495-500. Bibcode:2009Natur. ...
... is also used as an attachment substrate in embryonic stem cell culture. When embryonic stem cells are grown in the ... Likewise, test molecules that inhibit tumor cell migration may also have potential as anti-cancer drugs. Matrigel is also ... "The endothelial cell tube formation assay on basement membrane turns 20". Angiogenesis. 12 (3): 267-74. doi:10.1007/s10456-009- ... When used undiluted, Matrigel promotes stem cell growth and differentiation. Hughes, C.S., Postovit, L.M., Lajoie, G.A. (2010 ...
... peripheral primitive neuroectodermal tumor - peripheral stem cell - peripheral stem cell support - peripheral stem cell ... CEA assay - cecum - cefalexin - cefepime - cefixime - ceftriaxone - celecoxib - celiac disease - cell - cell differentiation - ... tumor load - tumor marker - tumor model - tumor necrosis factor - tumor suppressor gene - tumor-derived - tumor-specific ... stem cell - stem cell factor - stem cell transplantation - stent - stereotactic biopsy - stereotactic body radiation therapy - ...
... accelerated senescence and rapid malignant transformation of human fibroblasts through modulation of an embryonic stem cell ... Showe's research led to the creation of a blood test that could be used to test for lung cancer using a 29-gene assay. The ... Sp 100 as a potent tumor suppressor: ...
... neural stem cells and brain tumor stem cells. PneumaCult-ALI is a medium for the mucociliary differentiation of human bronchial ... Quantify Hematopoietic Progenitor Cells with the CFC Assay , STEMCELL Technologies mFreSR™ by STEMCELL Technologies Inc mFreSR ... Embryonic Stem Cells , STEMCELL Technologies Researchers use mTeSR1 medium for deriving human induced pluripotent stem cells BD ... STEMCELL Technologies ES-Cult™ - Reagents for Mouse Induced Pluripotent Stem Cells (iPSCs) & Embryonic Stem Cells (ESCs) , ...
"Enumeration of Neural Stem and Progenitor Cells in the Neural Colony-Forming Cell Assay". Stem Cells. 26 (4): 988-96. doi: ... doi:10.4103/0973-1482.80463 Intensity-modulated radiation to spare neural stem cells in brain tumors: a computational platform ... both daughter cells are also stem cells. In asymmetric division, a stem cells produces one stem cell and one specialized cell. ... "FoxO3 regulates neural stem cell homeostasis". Cell Stem Cell. 5 (5): 527-539. doi:10.1016/j.stem.2009.09.014. PMC 2775802 . ...
Since many tumor cells won't grow colonies in culture, cell proliferation assay, which has an satisfactory accuracy reportedly ... doi:10.1002/stem.5530050202. Niyazi, Maximilian; Niyazi, Ismat; Belka, Claus (2007). "Counting colonies of clonogenic assays by ... "The Human Tumor Clonogenic Assay as a Model System in Cell Biology". The International Journal of Cell Cloning. 5 (2): 89-107. ... human tumor cells are a typical choice. The cells either come from prepared "cell lines," which have been well-studied and ...
Mayani H (Mar 2010). "Biological differences between neonatal and adult human hematopoietic stem/progenitor cells". Stem Cells ... "Tumor protein 53-induced nuclear protein 1 expression is repressed by miR-155, and its restoration inhibits pancreatic tumor ... of endogenous transcript regulation by miR-155-5p and validation of the miR-155-5p seed sequence through a reporter assay was ... leukemic cells to healthy B cells and promote their transformation to tumorigenic-like cells Selection of competent B cells ...
"High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro ... assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell ... "Tumor-specific CD4+ T cells have a major "post-licensing" role in CTL mediated anti-tumor immunity". Journal of Immunology. 165 ... hematopoietic stem cells[16] and even bacteria.[17] Another novel application of CFSE is its use for the in vitro and in vivo ...
Cell Stem Cell. 20 (2): 191-204.e5. doi:10.1016/j.stem.2016.10.018. ISSN 1934-5909. PMID 27889319. Thomson, Sophie R.; Seo, ... IdentiGen SNP Genotyping Assay Chemistry; NuGen Target Enrichment". GenomeWeb. Retrieved 2017-11-03. "Circulating Tumor DNA ... "Cell-Type-Specific Chromatin States Differentially Prime Squamous Cell Carcinoma Tumor-Initiating Cells for Epithelial to ... Yang, Yi-Lin; Buck, Gregory A.; Widmer, Giovanni (1 March 2010). "Cell Sorting-Assisted Microarray Profiling of Host Cell ...
Unlike chemotherapy the affects may not be due to direct destruction of stem cells but the results may be equally serious. The ... In-vitro colony forming cell (CFC) assays using normal human bone marrow grown in appropriate semi-solid media such as ... "Granulocyte colony-stimulating factor enhances bone tumor growth in mice in an osteoclast-dependent manner". Blood. 109 (8): ... These predictive in-vitro assays reveal effects the administered compounds have on the bone marrow progenitor cells that ...
... granule Specol STAT protein STAT1 STAT2 STAT3 STAT4 STAT5 STAT6 Stem cell educator Stimulator of interferon genes Stromal cell- ... Secretion assay Self-protein Sensitization (immunology) Sentinel cell Serum amyloid A Short course immune induction therapy ... TPBG Transfer factor TRIF TRIM5alpha Trogocytosis Tuberculin Tumor necrosis factor alpha Tumor necrosis factor receptor Tumor ... CCR9 CCRL1 CCRL2 CD117 CD32B CD4 CD4+ T cells and antitumor immunity CD74 CD94/NKG2 Cell-mediated immunity CELSR1 Central ...
Careful assays for cancer stem cells". Nature Reports Stem Cells. doi:10.1038/stemcells.2009.47. Dick, J (2013). "Q&A: John ... o'Brien, C. A.; Pollett, A.; Gallinger, S.; Dick, J. E. (2006). "A human colon cancer cell capable of initiating tumour growth ... "Cancer Stem Cells--Perspectives on Current Status and Future Directions: AACR Workshop on Cancer Stem Cells". Cancer Research. ... This time he presented it as the "cancer stem-cell hypothesis". His model stated that there are different cancer cells and ...
... stem cells, white blood cells) in many tissues and organs. SP amplifies or excites most cellular processes.[15][16] ... When assayed in the human, the observed variability of the SP concentrations are large, and in some cases the assay methodology ... a potential target for novel medicines in malignant brain tumour therapies (mini-review)". Folia Neuropathologica. 45 (3): 99- ... Substance P has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ...