Loading...
*  Molecular cloning
Both transformation and transfection usually require preparation of the cells through a special growth regime and chemical ... In mammalian cell culture, the analogous process of introducing DNA into cells is commonly termed transfection. ... transfection, electroporation). When microorganisms are able to take up and replicate DNA from their local environment, the ...
*  Transfection
"Nanoparticle Based Transfection Reagents". Biology Transfection Research Resource. Transfection.ws. Graham FL, van der Eb AJ ( ... Other methods of transfection include nucleofection, which has proved very efficient in transfection of the THP-1 cell line, ... Magnetofection, or magnet-assisted transfection, is a transfection method that uses magnetic force to deliver DNA into target ... Transfection at the US National Library of Medicine Medical Subject Headings (MeSH) "Transfection". Protocols and Applications ...
*  RNA transfection
"Long-RNA transfection" below). Short-RNA transfection is routinely used in biological research to knock down the expression of ... RNA transfection is the process of deliberately introducing RNA into a living cell. RNA can be purified from cells after lysis ... Long-RNA transfection is the process of deliberately introducing RNA molecules longer than about 25nt into living cells. A ... Angel M, Yanik MF (2010). "Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins". ...
*  Optical transfection
A general term that also encompasses optical transfection. Optical transfection (or any derivations of laser transfection, ... The meaning of the term transfection has evolved. The original meaning of transfection was "infection by transformation", i.e. ... YAG to generate stable and transient transfection of normal rat kidney cells. Since this time, the optical transfection of a ... Because of this strict definition of transfection, optical transfection also refers only to the introduction of nucleic acid ...
*  Reverse transfection
... storage without apparent loss of transfection efficiency. The disadvantages of reverse transfection are: Reverse transfection ... The advantages of reverse transfection (over conventional transfection) are: The addition and attachment of target cells to the ... Second, 200ul of transfection mix is pipetted into one of the HybriWell ports; the mixture will distribute evenly over the ... Third, the transfection mix is pipetted away and the HybriWell removed with a thin-tipped forceps. Fourth, the printed slide ...
*  Magnet-assisted transfection
... is a transfection method which uses magnetic interactions to deliver DNA into target cells. ... Magnet-assisted transfection is a relatively new and time-saving method to introduce nucleic acids into a target cell with ... Magnet-assisted transfection can also be performed in the presence of serum, which is a further benefit. Currently, there are ... A major advantage of the method is the mild treatment of the cells in comparison to liposome-based transfection reagents ( ...
*  Electroporation
... has proven efficient for use on tissues in vivo, for in utero applications as well as in ovo transfection. ... The process of introducing foreign DNA into eukaryotic cells is known as transfection. Electroporation is highly effective for ... Bioelectrochemistry 74, 124-129 (2008). Potter, Huntington; Heller, Richard (2003-05-01). "Transfection by Electroporation". ... providing researchers with an alternative to trypsinizing their cells prior to transfection. One downside to electroporation, ...
*  Altogen Biosystems
Ghule, Ajinkya (9 October 2017). "Global Transfection Technologies Market 2017- Bio-Rad, Polyplus Transfection, MaxCyte". ... Altogen Biosystems began as a company focusing on non-viral methods of transfection. The company began to experiment with a ... Altogen Biosystems has been named as one of the principal players in the transfection technology market by QY Research. Altogen ... Roberts, Josh P. (12 August 2014). "Primary Cells Pose No Problem with These Transfection Tools". BioCompare. Retrieved 29 ...
*  Lipofectamine
This method of transfection was invented by Dr. Yongliang Chu at Life Technologies, Inc. Lipofection Transfection Vectors in ... It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures ... But also in non-dividing cells, research has shown that Lipofectamine improves the efficiency of transfection, which suggests ... Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and ...
*  Small interfering RNA
The most common commercial reagents for transfection of siRNA are Lipofectamine and Neon Transfection. However it is not ... Gene knockdown by transfection of exogenous siRNA is often unsatisfactory because the effect is only transient, especially in ... siRNAs can also be introduced into cells by transfection. Since in principle any gene can be knocked down by a synthetic siRNA ... Once the siRNA is configured against the gene it has to be effectively delivered through a transfection protocol. Delivery is ...
*  Hexadimethrine bromide
"Polybrene Infection / Transfection Reagent , TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02. ...
*  CellSqueeze
Transfection How It Works. SQZ Biotech. Retrieved on 2014-05-18. Sharei, Armon; Zoldan, Janet; Adamo, Andrea; Sim, Woo Young; ...
*  Diethylaminoethyl cellulose
It is added to solution containing DNA meant for transfection. It binds and interacts with negatively charged DNA molecules and ... Gulick, Tod (2003). "Transfection Using DEAE-Dextran". doi:10.1002/0471143030.cb2004s19. Ninfa, Alexander (2010). Fundamental ... This procedure is highly suited for transient transfection used for various molecular biology studies. DEAE-Sepharose, DEAE-650 ...
*  Mirus Bio
Membrane activity and transfection ability of amphipathic polycations as a function of alkyl group size. Bioconjug Chem. 2005 ... These innovations also served as the basis for the company's transfection formulations and nucleic acid labeling and ... Mirus Bio (formerly Mirus Bio Corporation), develops and manufactures transfection reagents and related products for life ... new chemistries for transfection [9]; development of a non-viral vector providing sustained liver-specific transgene expression ...
*  Listeria monocytogenes
Current transfection efficiency remains poor. One example of the successful use of L. monocytogenes in in vitro transfer ...
*  Designer baby
The second technique uses the transfection process. Stem cells obtained from the embryo during the blastocysts stage are ...
*  Transduction (genetics)
Transfection - means of inserting DNA into a cell. Transformation (genetics) - means of inserting DNA into a cell. Viral vector ... This plasmid is inserted (usually by transfection) into a producer cell together with other plasmids (DNA constructs) that ... so that simultaneous transfection of multiple plasmids is required to get infectious virions. Moreover, only the plasmid ...
*  Mycovirus
Moleleki, N.; van Heerden, S. W.; Wingfield, M. J.; Wingfield, B. D.; Preisig, O. (2003). "Transfection of Diaporthe perjuncta ... to several fungal species that are closely related to Cryphonectria parasitica using in vitro virus transfection techniques. ...
*  Viral transformation
The term can also be understood as DNA transfection using a viral vector. Viral transformation can occur both naturally and ... Transduction Transfection Transgenesis Baron, ed. by Samuel (1996). Medical microbiology (4. ed.). Galveston, Texas: Univ. of ...
*  Poly(amidoamine)
This could result in a PAMAM-DNA complex, which would make DNA transfection more efficient due to neutralization of the charges ... As with drug delivery applications, specific targeting of the transfection complex is ideal and must be explored as well. ... Although viral vectors can offer highly specific, high-efficiency transfection, the generation of such viruses is costly and ... Indeed, several reports have confirmed PAMAM dendrimers as effective DNA transfection agents. When the charge balance between ...
*  Hymenolepiasis
H. diminuta has an effective mechanism for interspecies transfection. Beetles prefer to ingest rat droppings infected with ...
*  Genetically modified virus
Johnston SA, Tang DC (1994). "Gene gun transfection of animal cells and genetic immunization". Methods in Cell Biology. 43 Pt A ...
*  Polyethylenimine
Poly(ethylenimine) was the second polymeric transfection agent discovered, after poly-l-lysine. PEI condenses DNA into ... Akinc, A; Thomas, M; Klibanov, AM; Langer, R (2004). "Exploring polyethylenimine-mediated DNA transfection and the proton ... http://www.polyplus-transfection.com/transfection-reagents/high-throughput-screening-jetpei/ http://www.wipo.int/pctdb/en/wo. ... 2003). "Synthesis of Linear Polyethylenimine Derivatives for DNA Transfection". Bioconjugate Chemistry. 14: 581-587. doi: ...
*  Vectors in gene therapy
The use of particle bombardment, or the gene gun, is another physical method of DNA transfection. In this technique, DNA is ... This is the simplest method of non-viral transfection. Clinical trials carried out of intramuscular injection of a naked DNA ... Previously, low levels of transfection and expression of the gene held non-viral methods at a disadvantage; however, recent ... Inorganics have also been shown to exhibit improved in vitro transfection for attached cell lines due to their increased ...
*  Advanced Microscopy Group
Software features include time-lapse, cell counting and transfection analysis. A key feature of the EVOS fluorescence ...