Transfection[edit]. Bacterial spheroplasts, with suitable recombinant DNA inserted into it, can be used to transfect animal ...
"Polyplus transfection". CNRS. "Les quinze lauréats de la Médaille d'argent du CNRS 1998". cnrs.fr. Archived from the original ... These vectors are widely used as transfection agents for animal cells in culture, but also as drug-gene carriers in clinical ... To this end, he founded two biotechnology companies, Eurothéra (1994-97) and Polyplus-transfection (2001). Research & Sharing ...
Kingston, Robert E.; Chen, Claudia A.; Rose, John K. (2003). "Calcium Phosphate Transfection". Current Protocols in Molecular ... lipid-mediated DNA-transfection procedure". Proceedings of the National Academy of Sciences. 84 (21): 7413-7417. doi:10.1073/ ...
Potter, H (2003). "Transfection by Electroporation". Current Protocols in Molecular Biology. Arena CB, Sano MB, Rossmeisl JH, ... Electroporation has proven efficient for use on tissues in vivo, for in utero applications as well as in ovo transfection. ... Particularly, the electroporation allows for a more efficient transfection of DNA, RNA, shRNA, and all nucleic acids into the ... The process of introducing foreign DNA into eukaryotic cells is known as transfection. Electroporation is highly effective for ...
... for he also developed two methods of calcium phosphate transfection for transient and stable transfections. These two methods ... Kingston, Robert E.; Chen, Claudia A.; Rose, John K. (2003). "Calcium Phosphate Transfection". Current Protocols in Molecular ... Robert Kingston's advancements within the field of biotechnology extend into developing eukaryotic cell transfection protocols ...
Fanelli A (2016). "Transfection: In Vitro Transfection". Retrieved 5 December 2017. Jensen K, Anderson JA, Glass EJ (April 2014 ... The most common commercial reagents for transfection of siRNA are Lipofectamine and Neon Transfection. However it is not ... Gene knockdown by transfection of exogenous siRNA is often unsatisfactory because the effect is only transient, especially in ... siRNAs transfection into cells typically lowers the expression of many genes, however, the upregulation of genes is also ...
"Polybrene Infection / Transfection Reagent , TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02. Hunkapiller, M. W.; Hood, ...
Gulick, Tod (2003). "Transfection Using DEAE-Dextran". Current Protocols in Cell Biology. 19: 20.4.1-20.4.10. doi:10.1002/ ... It is added to solution containing DNA meant for transfection. It binds and interacts with negatively charged DNA molecules and ... This procedure is highly suited for transient transfection used for various molecular biology studies. DEAE-Sepharose, DEAE-650 ...
Membrane activity and transfection ability of amphipathic polycations as a function of alkyl group size. Bioconjug Chem. 2005 ... These innovations also served as the basis for the company's transfection formulations and nucleic acid labeling and ... Mirus Bio LLC (formerly Mirus Bio Corporation), develops and manufactures transfection reagents, electroporation solutions and ... new chemistries for transfection [9]; development of a non-viral vector providing sustained liver-specific transgene expression ...
Malone RW, Felgner PL, Verma IM (August 1989). "Cationic liposome-mediated RNA transfection". Proceedings of the National ...
Current transfection efficiency remains poor. One example of the successful use of L. monocytogenes in in vitro transfer ...
... Transfection and Selection Data for HeLa Cells. *Rebecca Skloot, The Immortal Life of Henrietta Lacks book website with ...
Moleleki, N.; van Heerden, S. W.; Wingfield, M. J.; Wingfield, B. D.; Preisig, O. (2003). "Transfection of Diaporthe perjuncta ... to several fungal species that are closely related to Cryphonectria parasitica using in vitro virus transfection techniques. ...
"Gene transfer (transfection) methods in animals , Genetic Engineering and Biotechnology Gene Transfer Methods and Transgenic ... or plants the process is called transformation and when it is used to deliver genes to animals it is called transfection. This ...
"Gene transfer (transfection) methods in animals , Genetic Engineering and Biotechnology Gene Transfer Methods and Transgenic ... Kim, Tae Kyung; Eberwine, James H. (August 2010). "Mammalian cell transfection: the present and the future". Analytical and ... so the process used to insert foreign DNA into animal cells is usually called transfection. There are many ways to directly ... this process is known as stable transfection. To create transgenic animals the DNA must be inserted into viable embryos or eggs ...
"Production of high-titer helper-free retroviruses by transient transfection". Proc. Natl. Acad. Sci. USA. 90 (18): 8392-8396. ...
Single cell transfections are used to virtually transfer any type of mammalian cell into another using a syringe which creates ... Cuerrier, Charles M.; Lebel, Réjean; Grandbois, Michel (2007-04-13). "Single cell transfection using plasmid decorated AFM ...
"Stereoselective pH Responsive Peptide Dendrimers for siRNA Transfection". Bioconjugate Chemistry. 30 (8): 2165-2182. doi: ...
The term can also be understood as DNA transfection using a viral vector. Viral transformation can occur both naturally and ... Transduction Transfection Transgenesis Baron, ed. by Samuel (1996). "Effects on Cells". Medical microbiology (4. ed.). ...
"Fluorescent Peptide Dendrimers for siRNA Transfection: Tracking pH Responsive Aggregation, siRNA Binding, and Cell Penetration ... "Stereoselective pH Responsive Peptide Dendrimers for siRNA Transfection". Bioconjugate Chemistry. 30 (8): 2165-2182. doi: ...
This could result in a PAMAM-DNA complex, which would make DNA transfection more efficient due to neutralization of the charges ... As with drug delivery applications, specific targeting of the transfection complex is ideal and must be explored as well. ... Although viral vectors can offer highly specific, high-efficiency transfection, the generation of such viruses is costly and ... Indeed, several reports have confirmed PAMAM dendrimers as effective DNA transfection agents. When the charge balance between ...
"Lentiviral transfection with the PDGF-B gene improves diabetic wound healing". Plast. Reconstr. Surg. 116 (2): 532-8. doi ...
H. diminuta has an effective mechanism for interspecies transfection. Beetles prefer to ingest rat droppings infected with ...
Poly(ethylenimine) was the second polymeric transfection agent discovered, after poly-l-lysine. PEI condenses DNA into ... Akinc, A; Thomas, M; Klibanov, AM; Langer, R (2004). "Exploring polyethylenimine-mediated DNA transfection and the proton ... 2003). "Synthesis of Linear Polyethylenimine Derivatives for DNA Transfection". Bioconjugate Chemistry. 14 (3): 581-587. doi: ... for Transfection Purpose and Linear Pei Obtained with Such Method". Archived from the original on 2012-08-05. Steuerle, Ulrich ...
The use of particle bombardment, or the gene gun, is another physical method of DNA transfection. In this technique, DNA is ... This is the simplest method of non-viral transfection. Clinical trials carried out of intramuscular injection of a naked DNA ... Previously, low levels of transfection and expression of the gene held non-viral methods at a disadvantage; however, recent ... Inorganics have also been shown to exhibit improved in vitro transfection for attached cell lines due to their increased ...
Material intended for transfection is prepared similarly from high purity oleic acid. It was originally introduced into ... The pure material can also be used for the liposomal-transfection of DNA, RNA and other negatively charged molecules. The ... and Transfection;". Critical Reviews in Therapeutic Drug Carrier Systems. 21 (4): 257-317. doi:10.1615/ ...
She earned her PhD in 2010, "Optical sorting and photo-transfection of mammalian cells". She was a member of the SPIE students ... Optical sorting and photo-transfection of mammalian cells (Thesis). Dholakia, Kishan, Council for Scientific and Industrial ... "Graphene for improved femtosecond laser based pluripotent stem cell transfection". Journal of Biophotonics. 7 (5): 351-362. doi ...
Transfection is often accomplished by introducing vectors into a cell. Once internalized, the fusion protein nicks the target ...
Software features include time-lapse, cell counting and transfection analysis. A key feature of the EVOS fluorescence ...