Quantitation of sulfhydryls DTNB, Ellman's reagent (uses incorrect absorbance coefficient) (CS1 errors: periodical ignored, ... "Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4'-dithiodipyridine". Anal Bioanal Chem. 373 (4-5): ... "Quick measurement of protein sulfhydryls with Ellman's reagent and with 4,4′-dithiodipyridine". Analytical and Bioanalytical ... Ellman's reagent can be used for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological ...
doi:10.1021/bi011544w Kenyon, G.L. and Bruice, T.W. (1977). Novel sulfhydryl reagents. Methods In Enzymology 47, 407-430. doi: ...
The initials stand for "sulfhydryl reagent variable". These enzymes were named for their greater activity against cefotaxime ...
Ernst LA, Gupta RK, Mujumdar RB, Waggoner AS (Jan 1989). "Cyanine dye labeling reagents for sulfhydryl groups". Cytometry. 10 ( ... CYanine dyes Mujumdar B, Ernst A, Mujumdar SR, Lewis CJ, Waggoner AS (Mar 1993). "Cyanine dye labeling reagents: ... Reagents for the Labeling of Biomolecules". European Journal of Organic Chemistry. 2008 (12): 2107-2117. doi:10.1002/ejoc. ... or a maleimide group to react with a sulfhydryl group of cysteine residues. Cy5 is sensitive to its electronic environment. ...
"The inhibition of brain hexokinase by adenosinediphosphate and sulfhydryl reagents". Journal of Biological Chemistry 206, 1954 ...
The A-form of complex I is insensitive to sulfhydryl reagents. It was found that these conformational changes may have a very ... Treatment of the D-form of complex I with the sulfhydryl reagents N-Ethylmaleimide or DTNB irreversibly blocks critical ...
Meijer AJ, Groot GS, Tager JM (May 1970). "Effect of sulphydryl-blocking reagents on mitochondrial anion-exchange reactions ... DIC is inhibited by some dicarboxylate analogues, such as butylmalonate, as well as bathophenanthroline and thiol reagents such ...
It is often used as sulfhydryl reagent, enzyme inhibitor and radiation-protective agent. Thiols can be synthesized to ... Hassan, W. (1976). "Inhibition of erythrocyte sickling by cystamine, a thiol reagent". Proceedings of the National Academy of ... Cystamine dihydrochloride is a useful reagent to derivatize various polymer monoliths for hydrophilic interaction liquid ...
It is a thiolating reagent that reacts with primary amine groups, such as those of amino acids, to form sulfhydryl groups. 2- ... "Traut's Reagent Instructions" (PDF). Thermo Scientific. Retrieved 2015-07-08. (Chemical articles with multiple compound IDs, ... 2-Iminothiolane is a cyclic thioimidate compound also known as Traut's reagent. ... Iminothiolane reacts with primary amines efficiently at pH 7 to 9, creating amidine compounds with a sulfhydryl group. Thus it ...
Kaziro and his team were able to observe the effects of biotin and sulfhydryl binding reagents on the enzyme's function. Kaziro ...
... is a common reagent in organic synthesis. As stated earlier, the reagent is prone to attack by nucleophiles ... However, if the adjacent amino acid to methionine has a hydroxyl or sulfhydryl group, this group can react with the imine to ... Cyanogen bromide is often used to immobilize proteins by coupling them to reagents such as agarose for affinity chromatography ... Morris, Joel; Kovács, Lajos; Ohe, Kouichi (2015). "Cyanogen Bromide". Encyclopedia of Reagents for Organic Synthesis. pp. 1-8. ...
If no free sulfhydryl groups are available, lysines can be modified with various thiolation reagents (Traut's reagent, SAT(PEG4 ... Because free sulfhydryl groups are less prevalent on most proteins compared to primary amines, sulfhydryl biotinylation is ... Sulfhydryl-reactive groups such as maleimides, haloacetyls and pyridyl disulfides, require free sulfhydryl groups for ... sulfhydryls, carboxyls and carbohydrates are not available for labeling. These reagents rely on aryl azides, which become ...
... need for a thiol reagent like beta mercaptoethanol in the regeneration of the protein since such reagents weaken the sulfhydryl ...
... sulfhydryl reagents, as well as trypsin or chymotrypsin inhibitors. Proteinase K is used for the destruction of proteins in ... Proteinase K activity is unaffected by the sulfhydryl modifying reagents: para-chloromercuribenzoic acid (PCMB), N-alpha-tosyl- ... although presumably if these reagents were included alongside disulfide reducing reagents which exposed the typically- ...
... reagent strips MeSH D27.720.470.410.690 - reducing agents MeSH D27.720.470.410.700 - sulfhydryl reagents MeSH D27.720.470.410. ... indicators and reagents MeSH D27.720.470.410.080 - affinity labels MeSH D27.720.470.410.080.600 - photoaffinity labels MeSH ... D27.720.470.410.200 - chromogenic compounds MeSH D27.720.470.410.210 - cross-linking reagents MeSH D27.720.470.410.360 - ... reagent kits, diagnostic MeSH D27.720.470.410.680.680 - ...
... sulfhydryl, or Cu2+ or Co2+ reagents/compounds Enzyme catalyzes reaction best with branched chain amino acids (in order from ...
OPA is used in a very sensitive fluorogenic reagent for assaying amines or sulfhydryls in solution, notably contained in ... This pale yellow solid is a building block in the synthesis of heterocyclic compounds and a reagent in the analysis of amino ...
reagent A test substance that is added to a system in order to bring about a chemical reaction, or to see whether a reaction ... Also sulfhydryl, sulfanyl, and mercapto. titration A laboratory method of quantitative chemical analysis that is used to ... masking agent A reagent used in a chemical analysis which reacts with one or more other chemical species that may interfere in ... A reagent that causes such a reaction is called the precipitant, and the separable solid itself is the precipitate. More ...
The iTRAQ reagents are used to label peptides from different samples that are pooled and analyzed by liquid chromatography and ... a reactive iodoacetyl functional group for labeling free sulfhydryls (iodoTMT) and (3) reactive alkoxyamine functional group ... TMT reagents can be used to simultaneously analyze 2 to 11 different peptide samples prepared from cells, tissues or biological ... An isobaric tag for relative and absolute quantitation (iTRAQ) is a reagent for tandem mass spectrometry that is used to ...
"Cleavage Cocktails; Reagent B; Reagent H; Reagent K; Reagent L; Reagent R". AAPPTEC. Retrieved 13 November 2022. Dick F (1995 ... Cysteine has a very reactive sulfhydryl group on its side chain. A disulfide bridge is created when a sulfur atom from one ... Phosphonium reagents include PyBOP (HOBt) and PyAOP (HOAt). These reagents form the same active ester species as the ... it was found that this reagent was superior to other reagents with regards to yield and low epimerization. Solid supports for ...
... and the maleimide moiety of SMCC links to the free sulfhydryl group of DM1, forming a covalent bond between the antibody and ... a type of chemical reagent that contains two reactive functional groups, a succinimide ester and a maleimide. The succinimide ...
Phosgene directly reacts with amine, sulfhydryl, and alcohol groups, adversely affecting cell macromolecules and metabolism. ... is also used as an industrial reagent and building block in synthesis of pharmaceuticals and other organic compounds. Because ...
The term sulfhydryl- or mercapto- is used as a prefix, e.g. mercaptopurine. Many thiols have strong odors resembling that of ... Thiols, especially in the presence of base, are readily oxidized by reagents such as bromine and iodine to give an organic ... Sulfhydryl groups in the active site of an enzyme can form noncovalent bonds with the enzyme's substrate as well, contributing ... The −SH functional group itself is referred to as either a thiol group or a sulfhydryl group, or a sulfanyl group. Thiols are ...
... (SMCC) is a heterobifunctional amine-to-sulfhydryl crosslinker, ... is prepared using SMCC reagent. Hermanson, Greg (2013). Bioconjugate Techniques. Elsevier. p. 299-339. ISBN 978-0-12-382239-0 ...
Reagents that affect the making and breaking of S−S bonds are key, e.g., ammonium thioglycolate. The high disulfide content of ... Disulfide bonds are usually formed from the oxidation of sulfhydryl (−SH) groups, especially in biological contexts. The ... Reagents that deliver the equivalent of "RS+" react with thiols to give asymmetrical disulfides: RSH + R ′ SNR 2 ″ ⟶ RS − SR ... In effect, the disulfide bond is transferred from the protein to the reagent in two steps, both thiol-disulfide exchange ...
Smythe CV (1936). "The reactions of Iodoacetate and of Iodoacetamide with various Sulfhydryl groups, with Urease, and with ... "Systematic Evaluation of Protein Reduction and Alkylation Reveals Massive Unspecific Side Effects by Iodine-containing Reagents ...
"TLC Visualization Reagents" (PDF). École Polytechnique Fédérale de Lausanne. Archived from the original (PDF) on 13 May 2015. ... Next, sodium nitroprusside is added to the solution and it reacts with the newly freed sulfhydryl groups. The test will turn a ... Sodium nitroprusside is also used as an analytical reagent under the name sodium nitroferricyanide for the detection of methyl ...
All have a sulfhydryl group (-SH) covalently bonded to an aromatic ring. The organosulfur ligand in the medicine thiomersal is ... Encyclopedia of Reagents for Organic Synthesis. New York: John Wiley & Sons. doi:10.1002/047084289X. hdl:10261/236866. ISBN ... cuprate Reagents: tert-Butyl Phenyl Ketone". Organic Syntheses.{{cite journal}}: CS1 maint: multiple names: authors list (link ...
This cysteine residue contains a sulfhydryl functional group which allows the peptide to be easily conjugated to a carrier ... and eliminates handling of toxic reagents. Amino acid media is used with a variety of serum proteins, proteins, peptides, and ...
The terminal sulfhydryl group of the phosphopantetheine is the site of attachment of the growing polyketide chain. Located at ... is a much more practical method than synthesizing each product via chemical reactions in vitro due to the cost of reagents and ...