Substrate binding and specificity[edit]. The structures of several HAT domains bound to acetyl-CoA and histone substrate ... 4 Substrate binding and specificity *4.1 Lysine selectivity *4.1.1 GNAT family ... The formation of multisubunit complexes has been observed to modulate the substrate specificity of HATs.[10] In general, while ... For example, the lysine specificity of MYST family HATs toward their histone substrates becomes more restricted when they ...
Implications for substrate specificity". J. Biol. Chem. 273 (34): 21714-20. doi:10.1074/jbc.273.34.21714. PMID 9705307.. ...
Use of Oriented Peptide Libraries to determine phosphopeptide binding specificity and protein kinase substrate specificity[edit ... 2.2 Use of Oriented Peptide Libraries to determine phosphopeptide binding specificity and protein kinase substrate specificity ... This approach was used to characterize the substrate specificity of a large number of protein kinases. The kinase specificity ... P as a substrate, in fact required PtdIns(5)P as a substrate to produce PtdIns(4,5)P2.[34] Further research demonstrated that ...
Retrieved from Encyclopædia Britannica Online: [1] Miller FD, Chapman JL, Queener SW (1992). "Substrate specificity of ... This allows for the binding of the substrate ACV to the deprotonated thiol group of the cysteine residue. This ligation of the ... and two water molecules in the absence of a bound substrate. Just two histidine residues and one aspartic acid residue are ... "Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation". Nature. 387 (6635): ...
These enzymes halogenate without substrate specificity and regioselectivity. The first tryptophan 7-halogenase was isolated in ... These results suggest that for larger substrates, perhaps the entirety of the substrate molecule is not enclosed in the active ... Enzyme activity was evolved with a known substrate that bears structural similarity to the target substrate, then variants with ... Site-directed mutagenesis has also been employed for expanding substrate scope. Tryptophan substrate interacts with numerous ...
It is now known that isomerases have broad substrate specificity. Most pentoses and some hexoses are all substrates for D- ... Metal site 1 binds the substrate tightly, while metal site two binds the substrate loosely. Both share an acid residue Glutamic ... The first metal, mentioned earlier, coordinates to O3 and O4, and is used to dock the substrate. In the isomerization of xylose ... state consists of a high energy carbonium ion that is stabilized through all the metal interactions with the sugar substrate. ...
Substrate specificities. Preferred cofactor. Catalytic preference. Tissue distribution. Expression profile. Pathology 1. ... HSD17B10: Also known as 2-methyl-3-hydroxybutyryl-CoA dehydrogenase (MHBD). Substrates include steroids, neurosteroids, fatty ...
A second precaution with respect to predicting FMO enzyme substrate specificity is that factors other than size and charge must ... substrate specificity and role in drug metabolism". Curr. Drug Metab. 1 (2): 181-191. doi:10.2174/1389200003339135. PMID ... This enzyme has a wide substrate specificity, including the dietary-derived tertiary amines trimethylamine, tyramine and ... Other drug substrates have been used for both in vitro and in vivo analyses. ... FMO3 is the most abundantly expressed FMO in ...
... comparison of its substrate specificity with that of other SAP kinases". The EMBO Journal. 16 (12): 3563-71. doi:10.1093/emboj/ ... Dual specificity mitogen-activated protein kinase kinase 6 also known as MAP kinase kinase 6 (MAPKK 6) or MAPK/ERK kinase 6 is ... MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase ... "Nuclear export of the stress-activated protein kinase p38 mediated by its substrate MAPKAP kinase-2". Current Biology. 8 (19): ...
"Dynamin is a minibrain kinase/dual specificity Yak1-related kinase 1A substrate". The Journal of Biological Chemistry. 277 (20 ... "Specificity of the binding of synapsin I to Src homology 3 domains". The Journal of Biological Chemistry. 275 (38): 29857-67. ... bind to the proline-rich region of synaptojanin 1 at distinct sites that display an unconventional binding specificity". The ...
Villiers BR, Hollfelder F (March 2009). "Mapping the limits of substrate specificity of the adenylation domain of TycA". ... whereas the ability to act upon different substrates is called substrate promiscuity or substrate ambiguity. The term latent ... Enzymes are evolved to catalyse a particular reaction on a particular substrate with a high catalytic efficiency (kcat/KM, cf. ... When the specificity of enzyme was probed, it was found that it was highly selective against natural amino acids that were not ...
In addition to controlling activity, the allosteric mechanism also regulates the substrate specificity and ensures the enzyme ... RNR1 contains both allosteric sites, mediating regulation of substrate specificity and activity. Depending on the allosteric ... Reduction of NDP substrates occurs under aerobic conditions. Class I reductases are divided into IA and IB due to differences ... The substrates for RNR are ADP, GDP, CDP and UDP. dTDP (deoxythymidine diphosphate) is synthesized by another enzyme ( ...
Specificity[edit]. Proteolysis can be highly promiscuous such that a wide range of protein substrates are hydrolysed. This is ... Promiscuous proteases typically bind to a single amino acid on the substrate and so only have specificity for that residue. For ... TopFIND - database of protease specificity, substrates, products and inhibitors. *MEROPS - Database of protease evolutionary ... have high specificity and only cleave very restricted set of substrate sequences. They are therefore a common target for ...
However, differences exist which unsurprisingly account for differences in substrate specificity among peroxidases. This ... implicating this residue or its peculiar functional group in substrate specificity. Cyanide binds very tightly to mammalian ... The distal face is used for substrate binding and catalysis. The crystal structures of MPO have been solved both in native ... This process operates on the majority of non-halide substrates. The first step is identical followed by: [Fe(IV)=O...Por•+] + ...
Basis for the dual substrate specificity and activation of the oncolytic agent tiazofurin". J. Biol. Chem. 277 (15): 13148-54. ...
"The SPOT technique as a tool for studying protein tyrosine phosphatase substrate specificities". Protein Sci. 11 (10): 2326-34 ...
Kim, S T; Lim D S, Canman C E, Kastan M B (1999). «Substrate specificities and identification of putative substrates of ATM ... Rodriguez, Maria; Yu Xiaochun, Chen Junjie, Songyang Zhou (2003). «Phosphopeptide binding specificities of BRCA1 COOH-terminal ... Nucleophosmin/B23 is a candidate substrate for the BRCA1-BARD1 ubiquitin ligase». J. Biol. Chem. 279 (30): 30919-22. PMID ...
"Fatty acid substrate specificities of human prostaglandin-endoperoxide H synthase-1 and -2. Formation of 12-hydroxy-(9Z, 13E/Z ... "Substrate Specificity Changes for Human Reticulocyte and Epithelial 15-Lipoxygenases Reveal Allosteric Product Regulation". ... actually prefers as its substrate the 18 carbon polyunsaturated fatty acid, linoleic acid, over arachidonic acid, converting it ...
Ziegler, D (2002). "An overview of the mechanism, substrate specificities, and structure of FMOs". Drug Metabolism Reviews. 34 ... doi:10.1016/0165-6147(90)90235-Z. Ziegler DM (August 2002). "An overview of the mechanism, substrate specificities, and ... In the presence of a substrate (S), a nucleophilic attack occurs on the distal O-atom of the prosthetic group. The substrate is ... There are several drugs reported to be typical substrates for FMOs. The majority of drugs function as alternate substrate ...
Examination of the substrate specificity of heparin and heparan sulfate lyases. Biochemistry. 1990, 29 (10): 2611-2617. PMID ... Specificity studies on the heparin lyases from Flavobacterium heparinum. Biochemistry. 1993, 32 (32): 8140-8145. PMID 8347612. ... Heparin enhances the specificity of antithrombin for thrombin and factor Xa independent of the reactive center loop sequence. ... Evidence for an exosite determinant of factor Xa specificity in heparin-activated antithrombin. J. Biol. Chem. 2001, 276 (18): ...
Several isozymes are encoded by different genes, which vary in cellular location and substrate specificity. Glutathione ... whose preferred substrate is hydrogen peroxide. Glutathione peroxidase 4 (GPx4) has a high preference for lipid hydroperoxides ...
"Cloning and substrate specificity of a human phenol UDP glucuronosyltransferase expressed in COS-7 cells". David Harding, ... These reactions are catalysed by a large group of broad-specificity transferases. UGT1A6 is a human gene encoding a phenol UDP ... The enzyme encoded by the gene UGT1A8 has glucuronidase activity with many substrates including coumarins, anthraquinones and ...
... topology and substrate specificity". International Journal for Parasitology. 41: 429-438. doi:10.1016/j.ijpara.2010.11.002.. ...
Lazcano A, Diaz-Villgomez E, Mills T, Oro J (March 1995). "On the levels of enzymatic substrate specificity: Implications for ... This facilitates the diffusion of indole formed at α active sites directly to β active sites in a process known as substrate ... It was also the first identified to utilize substrate channeling. As such, this enzyme has been studied extensively and is the ... Huang X, Holden HM, Raushel FM (2001). "Channeling of Substrates and Intermediates in Enzyme-Catalyzes Reactions". Annu Rev ...
de 1999). «The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases». Nat. Cell ... de 1994). «KAP: a dual specificity phosphatase that interacts with cyclin-dependent kinases». Proc. Natl. Acad. Sci. U.S.A. ( ...
When a substrate is thus "poly-ubiquitinated", it is targeted for destruction by the proteasome. Particular enzymes in the ... ubiquitin/proteasome pathway allow ubiquitination to be directed to some proteins but not others - specificity is gained by ... are targeted for destruction by the ligation of at least four copies of a small peptide called ubiquitin onto a substrate ... coupling targeted proteins to an "E3 ubiquitin ligase". Each E3 ubiquitin ligase binds to a particular set of substrates, ...