... a mass spectrometry approach". Journal of the American Society for Mass Spectrometry. 21 (9): 1633-42. doi:10.1016/j.jasms. ... Chen BH, Wang CC, Lu LY, Hung KS, Yang YS (Feb 2013). "Fluorescence assay for protein post-translational tyrosine sulfation". ... the crystallized structure of the catalytic region of TPST-2 and different experiments other methods using mass spectrometry ...
Automated analytical methods such as enzyme multiplied immunoassay technique or fluorescence polarization immunoassay are ... blood or plasma using versatile methods such as liquid chromatography-mass spectrometry or gas chromatography-mass spectrometry ...
2005) Red tide detection and tracing using MODIS fluorescence data: A regional example in SW Florida coastal waters, Remote ... and liquid chromatography mass spectrometry (LCMS) have been developed for detecting brevetoxin in shellfish, are more ... "Detection of Karenia brevis blooms on the west Florida shelf using in situ backscattering and fluorescence data". Harmful Algae ... brevis by making use of its chlorophyll fluorescence and low backscattering characteristics. In addition to methods of ...
In mice, smURFP fluorescence is visible in HT1080 tumor xenografts without exogenous biliverdin, but fluorescence is less than ... Mass spectrometry showed that the ester groups were rapidly removed from biliverdin dimethyl ester. Addition of 25 μM ... Visible fluorescence is not always usable fluorescence and fluorescent proteins should always be compared to other useful, ... and fluorescence is turned "on" with covalent attachment to the smURFP-tag to allow far-red fluorescence tracking of cargo ...
One specific method is called Laser-induced Fluorescence Spectroscopy, and uses spectroscopic methods to be able to detect what ... List of spectroscopists Mass spectrometry History of quantum mechanics Fraunhofer, J. (1817). "Bestimmung des Brechungs- und ...
Raicu V, Singh DR (November 2013). "FRET spectrometry: a new tool for the determination of protein quaternary structure in ... Fluorescence polarization (measures the rotational diffusion constant) Dielectric relaxation (measures the rotational diffusion ... Ultracentrifugation Surface-induced dissociation mass spectrometry Coimmunoprecipation FRET Nuclear Magnetic Resonance (NMR) ... such as MALDI-TOF mass spectrometry and SDS-PAGE) are generally not useful, since non-native conditions usually cause the ...
1991). "Imaging cytometry by multiparameter fluorescence". Cytometry. 12 (7): 579-96. doi:10.1002/cyto.990120702. PMID 1782829 ... "Empirical Evaluation of the Use of Computational HLA Binding as an Early Filter to the Mass Spectrometry-Based Epitope ...
Sparkman, O. D., Penton, Z. E., Kitson, F. G. (2011). "Gas Chromatography and Mass Spectrometry". In Sparkman, O. D., Penton, Z ... X-ray fluorescence (XRF), and high magnification light microscopy The main chromatographic technique utilised for the ... Since the 1950s, mass spectrometry (MS)-based techniques have been used to characterise lipids, proteins and metabolites. The ... This is achieved using Gas Chromatography-Mass Spectrometry (GC-MS) alongside other methods, including isotope ratio mass ...
Fluid statics Fluid theory of electricity Fluidics Fluidyne engine Fluid-structure interaction Fluorescence Fluorescence ... sensor Flow separation Flow through nozzles Flow tracer Flow velocity Flow visualization Flowing-afterglow mass spectrometry ...
Typically one binding partner is labeled with a fluorescence probe (although sometimes intrinsic protein fluorescence from ... Chemical cross-linking followed by high mass MALDI mass spectrometry can be used to analyze intact protein interactions in ... With fluorescence correlation spectroscopy, one protein is labeled with a fluorescent dye and the other is left unlabeled. The ... The increase in the polarization of the fluorescence upon binding of the labeled protein to its binding partner can be used to ...
Mermet, J. M. (2005). "Is it still possible, necessary and beneficial to perform research in ICP-atomic emission spectrometry ... fluorescence, and flame emission spectroscopy: a practical approach. New York: Wiley. ISBN 0-470-26478-0. Uden, Peter C. (1992 ... total carbon in natural waters by Reagent-Free Ion Chromatography and inductively coupled plasma atomic emission spectrometry ...
... or dark pigments were removed from the plaster statue and beads and used for X-Ray Fluorescence (XRF) analysis, which provided ... Chemical analyses were done on most samples using Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES). The ...
Energy-Dispersive X-Ray Fluorescence) analyses; OM (Optical Microscopy) of cross sections and paint layers; PLM (Polarized ... Scanning Electron Microscope-Energy Dispersive Spectrometry). Extensive visitor observations and movement mapping have also ...
The atomic composition of particulate samples can be measured with techniques such as X-ray spectrometry. Nitrogen dioxide (NO ... Sulphur dioxide (SO2) is measured by fluorescence spectroscopy. This involves firing ultraviolet light at a sample of the air ... Gilfrich, J; Burkhalter, P; Birks, L (1973). "X-ray spectrometry for particulate air pollution-a quantitative comparison of ... Analytical chemistry techniques used for measuring pollution include gas chromatography; various forms of spectrometry, ...
Pierce Protein Assay Chemoproteomics Protein mass spectrometry Ong SE, Mann M (October 2005). "Mass spectrometry-based ... uses fluorescence-based labeling of the proteins prior to separation has increased the precision of quantification as well as ... Mass spectrometry is not inherently quantitative because of differences in the ionization efficiency and/or detectability of ... Mass spectrometry methods are more sensitive to difference in protein structure like post-translational modification and thus ...
Using Southern blotting and fluorescence in situ hybridization (FISH) on the genome, the researchers found no evidence of DNA ... They also performed mass spectrometry on the translated protein to verify that the chimeric RNA is translated into protein. ...
Finally, there are enzyme-based concepts that use the intrinsic absorbance or fluorescence of (fluorescence-labeled) enzymes as ... Cabañero, A. I.; Recio, J. L.; Rupérez, M. (2006). "Liquid chromatography coupled to isotope ratio mass spectrometry: a new ... by the consumption of oxygen using fluorescence-optical sensors. ... are often used in combination with mass spectrometry. Taking ...
Revenko, A. G.; Tsvetyansky, A. L.; Eritenko, A. N. (2022-08-01). "X-ray fluorescence analysis of solid-state films, layers, ... Scanning electron microscopy coupled with energy dispersive X-ray spectrometry (SEM-EDX, or SEM-EDS) allows to visualize the ... The most common non-destructive techniques include ultrasonic thickness measurement, X-ray fluorescence (XRF), X-Ray ... and Rutherford backscattering spectrometry (RBS). Various methods of Chromatography are also used, as well as thermogravimetric ...
This has an advantage over X-ray fluorescence methods of robotically detecting purity, since purer diamonds are less likely to ... Optical colour sorters (CCD colour camera) combine X-ray sorting technology and NIR (near infrared spectrometry) to pick out ...
Principle Fluorescence microscope Yeast membrane protein imaging Dividing cell fluorescence HeLa cancer cells FISH fluorescence ... Gohlke, R; McLafferty, Fred W. (1993). "Early gas chromatography/mass spectrometry". Journal of the American Society for Mass ... Thompson N L 1991 Topics in Fluorescence Spectroscopy Techniques vol 1, ed J R Lakowicz (New York: Plenum) pp 337-78 Gohlke, R ... Magde D.; Elson E. L.; Webb W. W. (1974). "Fluorescence correlation spectroscopy II. An experimental realization, (1974)". ...
Other analytical methods including mass spectrometry and NMR have also been applied, and they are in particularly useful for ... A potentially high-throughput fluorescence-based assay has also been proposed by using a fluorinated-gamma-butyrobetaine analog ... "Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase". ChemBioChem. 13 ...
Usually, the secondary antibody is tagged by a fluorescence label that can be detected by a fluorescence scanner. Other label- ... More recently, label-free detection including surface plasmon resonance (SPR) spectroscopy, mass spectrometry (MS) and many ... "Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence- ...
... fluorescence polarization, fluorescence-quenching, mobility shift, bead-based detection, and cell-based formats. Protein ... Mass spectrometry is ideally suited for such analyses using HCD or ETD fragmentation, as the addition of phosphorylation ... However, the analysis of phosphorylated peptides by mass spectrometry is still not as straightforward as for "regular", ... There are also several quantitative protein phosphorylation methods, including fluorescence immunoassays, Microscale ...
Liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry". Journal of Chromatography A. 976 (1-2 ... X-ray fluorescence spectroscopy, infrared spectroscopy, Raman spectroscopy, nuclear magnetic resonance spectroscopy, ... Examples of hyphenated techniques: Gas chromatography-mass spectrometry (GC-MS) Liquid chromatography-mass spectrometry (LC-MS ... Ion-mobility spectrometry-mass spectrometry Prolate trochoidal mass spectrometer The visualization of single molecules, single ...
... mass spectrometry - x-ray diffraction - Southern blot - fractionation - Gram stain - Surface Plasmon Resonance - Microscale ... fluorescence Photosynthesis : light reaction - dark reaction Fermentation : Acetyl-CoA - lactic acid Cellular respiration : ... Western blotting Chromatography ELISA X-ray crystallography NMR Electron microscopy Molecular dynamics Mass spectrometry ...
Here he began working on laser-induced fluorescence (LIF) to study the rovibronic coupling in cyano radicals. He was the first ... Pittsburgh he worked with Wade Fite and Ted Brackman on the detection of electron impact on molecules using mass spectrometry. ... When the excited molecule fluoresced back to the ground state, the fluorescence was captured in a photomultiplier tube. He has ... Jackson, William M. (1973-07-15). "Laser induced fluorescence of CN radicals". The Journal of Chemical Physics. 59 (2): 960-961 ...
Time-resolved fluorescence spectroscopy is an extension of fluorescence spectroscopy. Here, the fluorescence of a sample is ... Time-resolved mass spectrometry Ultrafast laser spectroscopy Noda, I. (1993). "Generalized two-dimensional correlation method ... a short laser pulse acts as a gate for the detection of fluorescence light; only fluorescence light that arrives at the ... Stimulated emission follows the fluorescence spectrum of the molecule and is Stokes shifted relative to and often still ...
Nanotube fluorescence has been investigated for the purposes of imaging and sensing in biomedical applications. All nanotubes ... coupled with energy dispersive X-ray spectrometry analysis. NIST SRM 2483 is a soot of single-wall carbon nanotubes used as a ... Paul Cherukuri; Sergei M. Bachilo; Silvio H. Litovsky & R. Bruce Weisman (2004). "Near-Infrared Fluorescence Microscopy of ... Carbon nanotubes have useful absorption, photoluminescence (fluorescence), and Raman spectroscopy properties. Spectroscopic ...
... tendencies in five main branches of atomic spectrometry (absorption, emission, mass, fluorescence and ionization spectrometry) ... In the latter case, the main division is between optical and mass spectrometry. Mass spectrometry generally gives significantly ... For atomic mass spectrometry, a sample must also be ionized. Vaporization, atomization, and ionization are often, but not ... Cold vapour atomic fluorescence spectroscopy Atomic spectral line Wikimedia Commons has media related to Atomic spectra. ...
... and finding the biomarker sabineamine in this sample with mass spectrometry, takes less than one second to perform. In late May ... and derive its sex from the reflected light using fluorescence spectroscopy within six days, thus complying to Germany's legal ...
Time-Resolved Total Internal Reflection Fluorescence Microscopy of Cultured Cells using a Tb-Chelate Label. Anal. Chim. Acta ... Selective Detection of Gas-Phase TNT by Integrated Optical Waveguide Spectrometry Using Molecularly Imprinted Sol-Gel Sensing ... Fabrication and Characterization of Uranium Oxide Doped Sol-Gel Planar Waveguides for Attenuated Total Reflectance Spectrometry ... Dipole Orientation Distributions in Langmuir-Blodgett Films by Planar Waveguide Linear Dichroism and Fluorescence Anisotropy. J ...