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*  ERCC1
The ERCC1-XPF complex is a structure-specific endonuclease. ERCC1-XPF does not cut DNA that is exclusively single-stranded or ... This deficiency prevents resumption of RNA synthesis on the template DNA strand subsequent to it receiving a transcription- ... single-stranded tails from DNA ends before rejoining. This activity is needed during a single-strand annealing subpathway of ... but it cleaves the DNA phosphodiester backbone specifically at junctions between double-stranded and single-stranded DNA. It ...
*  Mung bean nuclease
A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA ... double-stranded RNA, or DNA / RNA hybrids. Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA ... The enzyme degrades single-stranded DNA or RNA to nucleoside 5'-monophosphates, but does not digest double-stranded DNA, ... Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA, and produces mono- and oligonucleotides ...
*  Mung bean endonuclease
... is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from ... the ends of DNA and RNA molecules, leaving blunt, ligatable ends. http://www.neb.com/nebecomm/products/productm0250.asp http:// ...
*  DNA/RNA non-specific endonuclease
... they act on both DNA and RNA, cleave double-stranded and single-stranded nucleic acids and require a divalent ion such as ... In molecular biology, enzymes in the DNA/RNA non-specific endonuclease family of bacterial and eukaryotic endonucleases EC 3.1. ... "Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided ...
*  Plectrovirus
The conversion from single-stranded to double-stranded form is carried out by the host's own DNA polymerase. The host's RNA ... This is initiated when a viral endonuclease (gp2) nicks the double stranded intermediate. This nicking site is specific and the ... These new single strand DNA sequences become templates for further DNA and RNA synthesis. When sufficient gp5 has accumulated ... gp9 and gp11 along with the single stranded DNA and gp%. It begins at a specific sequence within the DNA which is predicted to ...
*  Ribonuclease
RNase H is a ribonuclease that cleaves the RNA in a DNA/RNA duplex to produce ssDNA. RNase H is a non-specific endonuclease and ... It is specific for single-stranded RNAs. It cleaves the 3'-end of unpaired C and U residues, ultimately forming a 3'- ... EC number 3.1.??: RNase PhyM is sequence specific for single-stranded RNAs. It cleaves 3'-end of unpaired A and U residues. EC ... EC 3.1.27.4: RNase U2 is sequence specific for single-stranded RNAs. It cleaves 3'-end of unpaired A residues. EC 3.1.27.8: ...
*  Locked nucleic acid
Ideal for the detection of short RNA and DNA targets Increases the thermal stability of duplexes Capable of single nucleotide ... An LNA-specific molecular dynamics (MD) force field has been created which is able to accurately model LNA MD. Some of the ... discrimination Resistant to exo- and endonucleases resulting in high stability in vivo and in vitro applications Increased ... target specificity Facilitates Tm normalization Strand invasion properties enables detection of "hard to access" samples ...
*  Inovirus
These new single strand DNA sequences become templates for further DNA and RNA synthesis. When sufficient gp5 has accumulated ... gp9 and gp11 along with the single stranded DNA. It begins at a specific sequence within the DNA which is predicted to have a ... Genome replication is initiated when a viral endonuclease (gp2) nicks the double stranded intermediate. This nicking site is ... The genomes are circular, positive-sense, single-stranded DNA 4.4-8.5 kilobases in length. They encode 4 to 11 proteins. ...
*  Aspergillus nuclease S1
... single-stranded DNA specific endonuclease, single-strand-specific endodeoxyribonuclease, single strand-specific DNase and ... it can also occasionally introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. The enzyme ... Neurospora crassa single-strand specific endonuclease, S1 nuclease, single-strand endodeoxyribonuclease, ... Aspergillus nuclease S1 is an endonuclease enzyme derived from Aspergillus oryzae that splits single-stranded DNA (ssDNA) and ...
*  SMG6
... functions to bind single-stranded DNA in telomere maintenance and single-stranded RNA in nonsense-mediated mRNA decay (NMD ... SMG6 also functions as an endonuclease in the NMD pathway. The catalytic activity of SMG6 resides in its PIN domain, which is ... Differences in the orientation and specific residues in the TPR between SMG6 and its homologs may account for why SMG6 does not ... It has dual functions in telomere maintenance and RNA surveillance pathways. SMG6 binds single-stranded telomere DNA and ...
*  Innate immune system
For example, the Influenza A virus produces NS1 protein, which can bind to single-stranded and double-stranded RNA, thus ... that attack and destroy specific regions of the viral DNA of invading bacteriophages. Methylation of the host's own DNA marks ... Restriction endonucleases and the restriction modification system exist exclusively in prokaryotes. Invertebrates do not ... It is currently divided into two categories which are innate (non-specific) immunity and acquired (specific) immunity. Alberts ...
*  Primer (molecular biology)
In vivo DNA replication utilizes short strands of RNA called RNA primers to initiate DNA synthesis on both the leading and ... Cleavage of the RNA flaps involves either flap structure-specific endonuclease 1 (FEN1) cleavage of short flaps, or coating of ... end of the primer into a single-stranded RNA flap, which is removed by nuclease cleavage. ... as DNA-dependent DNA polymerase activity that copies the sense cDNA strand into antisense DNA to form a double-stranded DNA ...
*  Exonuclease
Each type of exonuclease has a specific type of function or requirement. Exonuclease I breaks apart single-stranded DNA in a 3 ... CCR4 has been found to contain RNA and single-stranded DNA 3' to 5' exonuclease activities. Another component associated with ... which is specific for double-stranded DNA RNase activity 3' phosphatase activity AP endonuclease activity (later found to be ... hydrolyzing enzyme that catalyzes linear double-stranded DNA and single-stranded DNA, which requires Ca2+. This enzyme is ...
*  No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing
One side of the ring is large enough to admit double stranded DNA, but the other end can only accommodate single stranded DNA, ... and multi-step recombination processes using endonucleases and ligases. Therefore, λ-red recombination is more specific in ... In DNA replication, RNA primers must be inserted along the lagging strand so that DNA polymerase is able to synthesize the ... Beta binds to the resulting single stranded 3' end and incorporates it into the target DNA to form the recombinant DNA. Phage λ ...
*  RecBCD
... either single- or double-stranded, from preparations of circular double-stranded DNA, since it requires a DNA end for activity ... Finally, RecBCD enzyme (perhaps the RecC subunit) recognizes a specific sequence in DNA, 5'-GCTGGTGG-3', known as Chi ( ... energy-dependent molecular motors that unwind DNA (or RNA in the case of other proteins). The RecB subunit in addition has a ... endonucleases, oxidative damage, and a host of other factors. The RecBCD enzyme is both a helicase that unwinds, or separates ...
*  Restriction enzyme
A restriction enzyme or restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition ... in which a sequence reads the same forward and backward on a single strand of DNA, as in GTAATG. The inverted repeat palindrome ... utilize guide RNAs to target specific non-palindromic sequences found on invading organisms. They can cut DNA of variable ... All types of enzymes recognize specific short DNA sequences and carry out the endonucleolytic cleavage of DNA to give specific ...
*  CRISPR
... creates a single or double strand break in the DNA. Properly spaced single strand breaks in the host DNA can trigger homology ... and structure-specific RNA processing by a CRISPR endonuclease". Science. 329 (5997): 1355-8. Bibcode:2010Sci...329.1355H. doi: ... binding double-stranded fragments of invading DNA, while Cas1 binds the single-stranded flanks of the DNA and catalyses their ... "single-guide RNA" that, when combined with Cas9, could find and cut the DNA target specified by the guide RNA. By manipulating ...
*  DNA base flipping
... has a complementary sequence to the sequence you would like to detect such that it binds to a single-strand of the DNA or RNA. ... Restriction endonucleases, also known as restriction enzymes are enzymes that cleave the sugar-phosphate backbone of the DNA at ... This enzyme recognizes a specific sequence of the DNA (5'-GCGC-3') and methylates the first cytosine base of the sequence at ... It can also occur in RNA double helices or in the DNA:RNA intermediates formed during RNA transcription. DNA base flipping ...
*  Southern blot
... separating it into single DNA strands for later hybridization to the probe (see below), and destroys any residual RNA that may ... Restriction endonucleases are used to cut high-molecular-weight DNA strands into smaller fragments. The DNA fragments are then ... The membrane is then exposed to a hybridization probe-a single DNA fragment with a specific sequence whose presence in the ... A probe that hybridizes only to a single DNA segment that has not been cut by the restriction enzyme will produce a single band ...
*  Index of molecular biology articles
DNA) - nick translation - Niemann-Pick disease, type C - non-coding DNA - non-coding strand - non-directiveness - ... single nucleotide polymorphism - siRNA - site-directed mutagenesis - site-specific recombination - slot blot - SNP - snRNA - ... untranslated RNA - upstream - upstream activator sequence - upstream DNA - upstream (transduction) - uracil - vector - Western ... endonuclease - enhancer - enzyme - epitope - ethidium bromide - evolutionary clock - evolutionary footprinting - exon - ...
*  Recombinase Polymerase Amplification
The RPA process employs three core enzymes - a recombinase, a single-stranded DNA-binding protein (SSB) and strand-displacing ... for rapid detection of viral genomic DNA or RNA, pathogenic bacterial genomic DNA, as well as short length aptamer DNA. The ... At optimal temperatures (37-42 °C), the reaction progresses rapidly and results in specific DNA amplification from just a few ... Addition of endonuclease IV means that a nfo probe can be used for lateral flow strip detection of successful amplification,. ...
*  Glyceraldehyde 3-phosphate dehydrogenase
GAPDH can self-associate into homotypic oligomers/aggregates GAPDH binds to single-stranded RNA and DNA and a number of nucleic ... to restart its endonuclease activity; Promyelocytic leukaemia protein (PML) in an RNA-dependent fashion; Rheb to sequester the ... In sperm, a testis-specific isoenzyme GAPDHS is expressed. Under normal cellular conditions, cytoplasmic GAPDH exists primarily ... Hepatitis B viral RNA, Hepatitis C viral RNA, HPIV3, lymphokine mRNA, IFN-γ mRNA, JEV mRNA, and telomeric DNA. Koningic acid ...
*  Nuclease
Short stretches of single stranded DNA containing such damaged nucleotide are removed from duplex DNA by separate endonucleases ... DNA mismatch repair in any given organism is effected by a suite of mismatch-specific endonucleases. In prokaryotes, this role ... The former acts on DNA, the latter on RNA. In the late 1960s, scientists Stuart Linn and Werner Arber isolated examples of the ... During DNA replication, DNA polymerases elongate new strands of DNA against complementary template strands. Most DNA ...
*  CRISPR interference
DNA heteroduplex ahead of RNA pol II when the sgRNA is complementary to exons of the template strand. In prokaryotes, this ... promote differentiation to a specific cell lineage. The ability to upregulate gene expression using dCas9-SunTag with a single ... "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity". Science. 337 (6096): 816-821. doi:10.1126/ ... The level of transcriptional repression for exonic sequences is strand-specific. sgRNA complementary to the non-template strand ...
*  Ribonuclease H
... single-stranded RNA genome into double-stranded DNA. This process requires three steps: first, RNA-dependent DNA polymerase ... is a family of non-sequence-specific endonuclease enzymes that catalyze the cleavage of RNA in an RNA/DNA substrate via a ... generating an RNA:DNA hybrid intermediate; second, the RNA strand is destroyed; and third, DNA-dependent DNA polymerase ... Ribonuclease H is a family of endonuclease enzymes with a shared substrate specificity for the RNA strand of RNA-DNA duplexes. ...
*  List of homing endonuclease cutting sites
"Site-specific DNA endonuclease and RNA maturase activities of two homologous intron-encoded proteins from yeast mitochondria". ... by a group I intron in the Chlamydomonas pallidostigmatica chloroplast small subunit rRNA gene introduces a single-strand break ... Nicking endonuclease: These enzymes cut only one DNA strand, leaving the other strand untouched. **: Unknown cutting site: ... "The single group-I intron in the chloroplast rrnL gene of Chlamydomonas humicola encodes a site-specific DNA endonuclease (I- ...
*  Gene drive
... either a homing endonuclease or a RNA-guided endonuclease (e.g., Cas9 or Cpf1) and its guide RNA, that cuts the target sequence ... At the molecular level, an endonuclease gene drive works by cutting a chromosome at a specific site that does not encode the ... Because the template must be used to repair a double-strand break at the cutting site, its sides are homologous to the ... Zimmer, Carl (July 17, 2014). "A Call to Fight Malaria One Mosquito at a Time by Altering DNA". The New York Times. Retrieved ...