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*  Molecular cloning
... antibody probes, polymerase chain reaction, restriction fragment analysis and/or DNA sequencing. Molecular cloning provides ... This process takes advantage of the fact that a single bacterial cell can be induced to take up and replicate a single ... This single cell can then be expanded exponentially to generate a large amount of bacteria, each of which contain copies of the ... Molecular cloning is similar to polymerase chain reaction (PCR) in that it permits the replication of DNA sequence. The ...
*  Intrabody (protein)
2009). "Single-chain intracellular antibodies inhibit influenza virus replication by disrupting interaction of proteins ... Auf Der Maur, A; Escher, D; Barberis, A (2001). "Antigen-independent selection of stable intracellular single-chain antibodies ... including the use of single-chain antibodies (scFvs), modification of immunoglobulin VL domains for hyperstability, selection ... In molecular biology, an intrabody (from intracellular and antibody) is an antibody that works within the cell to bind to an ...
*  Incheol Shin
"Proapoptotic Activity of Cell-Permeable Anti-Akt Single-Chain Antibodies". Cancer Research. Cancer Research. 65 (7): 2815-2824 ...
*  Fluorescent tag
April 2008). "Fluorogen-activating single-chain antibodies for imaging cell surface proteins". Nature Biotechnology (Abstract ... An antisense strand labeled with the fluorescent probe is attached to a single mRNA strand, and can then be viewed during cell ... The most commonly labelled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes ... Perrier, Aurélie; Maurel, François; Jacquemin, Denis (21 August 2012). "Single Molecule Multiphotochromism with Diarylethenes ...
*  MRNA display
Nine years later, Fukuda and colleagues chose mRNA display method for in vitro evolution of single-chain Fv (scFv) antibody ... 2006). "In vitro evolution of single-chain antibodies using mRNA display". Nucleic Acids Research. 34 (19): e127. doi:10.1093/ ... In this ligation step, a piece of mRNA is ligated with a single stranded DNA with the help from T4 DNA ligase. This is not a ... After translation, the single-stranded mRNA portions of the fusions will be converted to heteroduplex of RNA/DNA by reverse ...
*  Cn2 toxin (beta-mammal toxin Cn2)
Exploiting cross-reactivity to neutralize two different scorpion venoms with one single chain antibody fragment. J Biol Chem. ... Single-chain variable fragments (scFvs) have been used to recognize and neutralize Cn2 from Centruroides noxius venom. ... Cn2 toxin is a single chain β-scorpion neurotoxic peptide and the primary toxin in the venom of the Centruroides noxious ... Cn2 is generally categorized as a β-scorpion toxin composed of a single chain polypeptide consisting of 66 amino acids, with an ...
*  Oncolytic virus
Frentzen, A.; Yu, Y. A.; Chen, N.; Zhang, Q.; Weibel, S.; Raab, V.; Szalay, A. A. (2009). "Anti-VEGF single-chain antibody GLAF ... Alternatively, the viral vector can be coated with a polymer such as polyethylene glycol, shielding it from antibodies, but ... Vesicular stomatitis virus (VSV) is a rhabdovirus, consisting of 5 genes encoded by a negative sense, single-stranded RNA ... The anti-angiogenesis drug Bevacizumab (anti-VEGF antibody) has been shown to reduce the inflammatory response to oncolytic HSV ...
*  Genelux Corporation
2009). "Anti-VEGF single-chain antibody GLAF-1 encoded by oncolytic vaccinia virus significantly enhances antitumor therapy". ...
*  Affinity chromatography
Chromatography of Native and Recombinant Proteins from Receptors for Insulin and IGF-I to Recombinant Single Chain Antibodies ... to remove the undesirable anti-GST antibodies from the serum and to purify the target antibody. Monoclonal antibodies can also ... This allows any antibodies that recognize the antigen to be captured on the solid support. Elution of the antibodies of ... This will remove antibodies against the GST part of the fusion protein. The serum is then separated from the solid support and ...
*  Kim Janda
"A Human Single-Chain Antibody Specific for Integrin α3β1 Capable of Cell Internalization and Delivery of Antitumor Agents". ... "Phage-Display Selection of a Human Single-Chain Fv Antibody Highly Specific for Melanoma and Breast Cancer Cells Using a ... prepared cell-surface glycosphingolipid can be utilized as a panning reagent to identify fully human single chain antibodies ( ... Gao, C.; Mao, S.; Lo, C.-H. L.; Wirsching, P.; Lerner, R. A.; Janda, K. D. (25 May 1999). "Making artificial antibodies: A ...
*  LY75
1999). "Intrathymic function of the human cortical epithelial cell surface antigen gp200-MR6: single-chain antibodies to ... 2000). "A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells". Hum. Immunol. 61 (8): 729-38. doi: ...
*  Sublingual administration
Fab fragments or single chain antibodies) is sublingual administration. Peptides and proteins are not stable in the gastro- ... A relatively new way of administration of therapeutic peptides and proteins (such as cytokines, domain antibodies, ...
*  CEACAM5
Boehm MK, Perkins SJ (2000). "Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody ...
*  Carcinoembryonic antigen
"Structural models for carcinoembryonic antigen and its complex with the single-chain Fv antibody molecule MFE23". FEBS Letters ... Because even monoclonal antibodies to CEA tend to have some degree of cross-reactivity, occasionally giving false positive ... Regions of high CEA levels in the body can be detected with the monoclonal antibody arcitumomab.[clarification needed] ... Antibodies to CEA are also commonly used in immunohistochemistry to identify cells expressing the glycoprotein in tissue ...
*  ImmTAC
... based targeting system with a single chain antibody fragment (scFv) effector function. TCRs, like antibodies, constitute an ... affinity enhanced TCR with a biological effector function the beta chain of the TCR is fused to an scFv antibody fragment ... This picomolar affinity binding results in the diseased cells becoming coated in CD3 co-receptor specific scFv antibody ... 2-chain membrane receptors expressed on the surface of T cells. To produce stable, soluble, high affinity TCRs capable of being ...
*  DsbC protein family
"Overexpression of DsbC and DsbG markedly improves soluble and functional expression of single-chain Fv antibodies in ... using this approach are the expression of reteplase in E.Coli and the functional expression of single chain Fv antibodies in E ...
*  TREML1
2007). "Inhibition of thrombin-induced platelet aggregation using human single-chain Fv antibodies specific for TREM-like ... TREML1 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM ... "The human TREM gene cluster at 6p21.1 encodes both activating and inhibitory single IgV domain receptors and includes NKp44". ...
*  Single-chain variable fragment
A single-chain variable fragment (scFv) is not actually a fragment of an antibody, but instead is a fusion protein of the ... Single-chain variable fragments lack the constant Fc region found in complete antibody molecules, and, thus, the common binding ... Le Gall, F.; Kipriyanov, SM; Moldenhauer, G; Little, M (1999). "Di-, tri- and tetrameric single chain Fv antibody fragments ... "Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue ...
*  Immunocontraception
Samuel, AS; RK Naz (2008). "Isolation of human single chain variable fragment antibodies against specific sperm antigens for ... All women generated antibodies against hCG, but only 119 (80%) generated antibody titers clearly above 50 ng/mL, which was the ... In passive immunity the desired antibody titers are achieved by injecting antibodies directly into an animal. The efficacy of ... anti-sperm antibodies are not likely to have adverse health effects, since anti-sperm antibodies are produced by up to 70% of ...
*  CD47
"A new disulfide-linked dimer of a single-chain antibody fragment against human CD47 induces apoptosis in lymphoid malignant ... Thus blocking CD47 with antibody turns off "don't eat me" signal and favors phagocytosis. Anti-CD47 antibody-mediated ... Noteworthy, anti-CD47 antibody treatment not only enables macrophage phagocytosis of cancer, but also fosters the activation of ... Humanized anti-CD47 antibody is being evaluated for the treatment of various cancers, particularly childhood brain tumors. ...
*  Bijvoet Center for Biomolecular Research
Another example is the development of so-called nanobullets, which are small antibody fragments, based on the single-chain ... including therapeutic antibodies. The center also works on the simulation of the interactions between proteins and their ... antibodies found in llamas to target chemotheurapeutics directly to cancer cells in a cancer patient. The previous two ...
*  Creative Biolabs
"Therapeutic anti-IgE monoclonal antibody single chain variable fragment (scFv) safety and immunomodulatory effects after one ... such as Anti-ADP3 chicken IgY Antibody libraries such as HuSdLTM phage display human single domain antibody library "Creative ... "Creative Biolabs Updates Its Antibody Humanization Service to Further Accelerate Your Monoclonal Antibody Research". EIN ... antibody sequencing, and antibody humanization. Customers include pharmaceutical companies, academic institutions, government ...
*  Scab
... single-chain antibody fragment Apple scab, an apple tree (genus Malus) fungal disease caused by Venturia inaequalis Black scab ...
*  Gabriella Campadelli-Fiume
A Herpes Simplex Virus Recombinant That Exhibits a Single-Chain Antibody to HER2/neu Enters Cells through the Mammary Tumor ...
*  Contrast-enhanced ultrasound
"Novel single-chain antibody-targeted microbubbles for molecular ultrasound imaging of thrombosis: Validation of a unique non- ... Since the antibodies are not humanized, they will elicit an immune response when used in human therapy. Humanizing antibodies ... Microbubbles can be conjugated to a recombinant single-chain variable fragment specific for activated glycoprotein IIb/IIIa ( ... Attempts have been made to outfit microbubbles with monoclonal antibodies that bind P-selectin, ICAM-1, and VCAM-1, but the ...
*  Phage display
Then the expression of single chain Fv's (scFv), and single chain T cell receptors (scTCR) were expressed both with and without ... Antibody phage display was later used by Carlos F. Barbas at The Scripps Research Institute to create synthetic human antibody ... HUMIRA, an antibody to TNF alpha, was the world's first fully human antibody, which achieved annual sales exceeding $1bn. Below ... The invention of antibody phage display revolutionised antibody drug discovery. Initial work was done by laboratories at the ...
*  Preclinical imaging
"Novel single-chain antibody-targeted microbubbles for molecular ultrasound imaging of thrombosis: Validation of a unique non- ... Antibody-bound paramagnetic nanoparticles can also be used to increase resolution and to visualize molecular expression in the ... Principle: Similar to PET, Single Photon Emission Computed Tomography (SPECT) also images living systems through γ-rays emitted ... and single photon emission computed tomography (SPECT) are usually used for molecular visualizations. These days, many ...