ATP), RNA ligase, polyribonucleotide ligase, and ribonucleic ligase. As of late 2007, two structures have been solved for this ... In enzymology, an RNA ligase (ATP) (EC 6.5.1.3) is an enzyme that catalyzes the chemical reaction ATP + (ribonucleotide)n + ( ... Silber R, Malathi VG, Hurwitz J (1972). "Purification and properties of bacteriophage T4-induced RNA ligase". Proc. Natl. Acad ... The systematic name of this enzyme class is poly(ribonucleotide):poly(ribonucleotide) ligase (AMP-forming). Other names in ...
... belongs to the RtcB family of ATP-dependent RNA ligases, named after the eponymous protein in E. coli. The bacterial RtcB ... Tanaka N, Shuman S (March 2011). "RtcB is the RNA ligase component of an Escherichia coli RNA repair operon". The Journal of ... RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene. It is found in the stress ... "Entrez Gene: RNA 2',3'-cyclic phosphate and 5'-OH ligase". Retrieved 2019-06-06. Jain S, Wheeler JR, Walters RW, Agrawal A, ...
... succinate-coa ligases MeSH D08.811.464.754.600 - dna ligases MeSH D08.811.464.754.720 - rna ligase (atp) MeSH D08.811.464.938. ... valine-tRNA ligase MeSH D08.811.464.267.500 - coenzyme a ligases MeSH D08.811.464.267.500.200 - acetate-coa ligase MeSH D08.811 ... rna polymerase i MeSH D08.811.913.696.445.735.270.762 - rna polymerase ii MeSH D08.811.913.696.445.735.270.775 - rna polymerase ... alanine-tRNA ligase MeSH D08.811.464.263.200.100 - arginine-tRNA ligase MeSH D08.811.464.263.200.150 - aspartate-tRNA ligase ...
... dogma of molecular biology Exonuclease Ligase Nuclease PCR PARP Reverse transcription polymerase chain reaction RNA ligase (ATP ... RNA polymerase I, RNA polymerase II, RNA polymerase III Single-subunit (ssDdRP): T7 RNA polymerase, POLRMT Primase, PrimPol RNA ... RNA-directed RNA polymerase, RdRP) Viral (single-subunit) Eukaryotic cellular (cRdRP; dual-subunit) Template-less RNA ... DNA polymerase and RNA polymerase are used to assemble DNA and RNA molecules, respectively, by copying a DNA template strand ...
The DNA ligase first reacts with ATP or NAD+, forming a ligase-AMP intermediate with the AMP linked to the ε-amino group of ... RNA may also be ligated similarly. A co-factor is generally involved in the reaction, and this is usually ATP or NAD+. ... Eukaryotic cells ligases belong to ATP type, and NAD+ - dependent are found in bacteria (e.g. E. coli). The discovery of DNA ... The smallest known eukaryotic ligase is Chlorella virus DNA ligase (ChVLig). It contains only 298 amino acids. When ChVLig is ...
ATP-dependent Clp protease ATP-binding subunit, DNA-directed RNA polymerase subunit beta, chorismate synthase, stage IV ... that are uniquely present in this family in the proteins phenylalanine-tRNA ligase subunit alpha, chaperonin GroEL, ribosome ... maturation factor RimP, BrxA/BrxB family bacilliredoxin, RNA methyltransferase, Rhomboid family intramembrane serine protease, ...
In enzymology, a RNA-3′-phosphate cyclase (EC 6.5.1.4) is an enzyme that catalyzes the chemical reaction ATP + RNA 3'-terminal- ... The systematic name of this enzyme class is RNA-3'-phosphate:RNA ligase (cyclizing, AMP-forming). This enzyme is also called ... the two substrates of this enzyme are ATP and RNA 3'-terminal-phosphate, whereas its 3 products are AMP, diphosphate, and RNA ... Filipowicz W, Konarska M, Gross HJ, Shatkin AJ (1983). "RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa ...
The three substrates of this enzyme are ATP, L-threonine, and threonine-specific transfer RNA [tRNA(Thr)], whereas its three ... In enzymology, a threonine-tRNA ligase (EC 6.1.1.3) is an enzyme that catalyzes the chemical reaction ATP + L-threonine + tRNA( ... Threonine-tRNA ligase (TARS) belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in tRNA and ... During their long evolution, some of these enzymes have acquired additional functions, including roles in RNA splicing, RNA ...
RNA helicase, tRNA pseudouridine(13) synthase TruD, glycine/betaine ABC transporter ATP-binding protein, superoxide dismutase, ... were identified through genomic analyses as exclusive for this family in the proteins glutamate-cysteine ligase, DNA gyrase ( ...
... such as CARM-1 and PRMT-1 RNA transcripts, such as SRA1 Cell cycle regulators such as cdc 25B RNA helicases such as p72 (DDX17 ... To name a few, classes of coactivators include: Acetyltransferases, such as members of the Src/NCOA family Ubiquitin ligases, ... such as E6-AP ATP-coupled chromatin remodeling complexes, such as the SWI/SNF/BRG-1 (SMARCA4) complex Protein methylases, ... functions as an RNA transcript and is present in an SRC-1 complex". Cell. 97 (1): 17-27. doi:10.1016/S0092-8674(00)80711-4. ...
... tRNACys ligase (AMP-forming). This enzyme catalyses the following chemical reaction: ATP + O-phospho-L-serine + tRNACys ⇌ {\ ... Fukunaga R, Yokoyama S (April 2007). "Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea ... O-phospho-L-serine-tRNA ligase (EC 6.1.1.27, O-phosphoseryl-tRNA ligase, non-canonical O-phosphoseryl-tRNA synthetase, SepRS) ... O-phospho-L-serine-tRNA+ligase at the U.S. National Library of Medicine Medical Subject Headings (MeSH) Portal: Biology (EC 6.1 ...
The three substrates of this enzyme are ATP, L-tyrosine, and a tyrosine-specific transfer RNA [tRNA(Tyr) or tRNATyr], whereas ... Tyrosine-tRNA ligase catalyzes the chemical reaction ATP + L-tyrosine + tRNA(Tyr) ⇌ {\displaystyle \rightleftharpoons } AMP + ... Tyrosine-tRNA ligase (EC 6.1.1.1), also known as tyrosyl-tRNA synthetase is an enzyme that is encoded by the gene YARS. ... The structure shows that the group I intron binds across the two subunits of the homodimeric protein with a newly evolved RNA- ...
... to combine it with glycine in a process driven by ATP. The reaction, EC 6.3.4.13 forms an amide bond: PRA + glycine + ATP → GAR ... GAR is the product of the enzyme phosphoribosylamine-glycine ligase acting on phosphoribosylamine (PRA) ... and hence is a building block for DNA and RNA. The vitamins thiamine and cobalamin also contain fragments derived from GAR. ...
Unlike E. coli DNA ligase, T4 DNA ligase cannot utilize NAD and it has an absolute requirement for ATP as a cofactor. Some ... DNA ligase I: ligates the nascent DNA of the lagging strand after the Ribonuclease H has removed the RNA primer from the ... DNA ligase is used in both DNA repair and DNA replication (see Mammalian ligases). In addition, DNA ligase has extensive use in ... units of ligase. The optimal incubation temperature for T4 DNA ligase is 16 °C. Bacteriophage T4 ligase mutants have increased ...
All of these enzymes have an ATP binding domain classified as ATP-grasp domains. In humans, the gene that codes for GARS-AIRS- ... In bacteria, GARS is a monofunctional enzyme (encoded by the purD gene). The purD genes often contain PurD RNA motif in their 5 ... The A and B domains appear to facilitate ATP binding, while the N and C domains confer substrate specificity. The N domain is ... The reaction starts with the oxygen of glycine acting as a nucleophile to attack the γ-phosphorus of ATP. Then, the nitrogen of ...
Ho, C. K.; Van Etten, J. L.; Shuman, S. (1997). "Characterization of an ATP-dependent DNA ligase encoded by Chlorella virus ... As PBCV-1 lacks an RNA polymerase gene, the virus must use the host cell's machinery to produce viral RNA. Thus, the viral DNA ... PBCV-1 also encodes other proteins involved in DNA replication including an ATP-dependent DNA ligase, a type II DNA ... Neither PBCV-1 nor ESV-1, for example encodes RNA polymerase components. Viral RNA polymerase genes are not transcribed until ...
... an SF2 ATPase that uses ATP hydrolysis to translocate on dsDNA upstream of the transcription bubble and forward translocate RNA ... DNA ligase I and Flap endonuclease 1 or the Ligase-III-XRCC1 complex seal the nicks to complete NER. The process of nucleotide ... Instead TC-NER initiates when RNA polymerase stalls at a lesion in DNA: the blocked RNA polymerase serves as a damage ... Final ligation to complete NER and form a double stranded DNA is carried out by DNA ligase. NER can be divided into two ...
RNA uridylyltransferase EC 2.7.7.53: ATP adenylyltransferase EC 2.7.7.54: The activity is part of EC 6.3.2.40, cyclopeptine ... glutamate-tRNA ligase, EC 1.2.1.70, glutamyl-tRNA reductase and EC 5.4.3.8 glutamate-1-semialdehyde 2,1-aminomutase EC 2.7.2.14 ... RNA-directed RNA polymerase EC 2.7.7.49: RNA-directed DNA polymerase EC 2.7.7.50: mRNA guanylyltransferase EC 2.7.7.51: ... ATP) EC 2.7.1.226: L-serine kinase (ADP) EC 2.7.1.227: inositol phosphorylceramide synthase EC 2.7.1.228: mannosyl-inositol- ...
DNA ligase (ATP) EC 6.5.1.2: DNA ligase (NAD+) EC 6.5.1.3: RNA ligase (ATP) EC 6.5.1.4: RNA 3′-terminal-phosphate cyclase (ATP ... EC 6.5.1.5: RNA 3′-terminal-phosphate cyclase (GTP) * EC 6.5.1.6: DNA ligase (ATP or NAD+) * EC 6.5.1.7: DNA ligase (ATP, ADP ... glutarate-CoA ligase EC 6.2.1.7: cholate-CoA ligase EC 6.2.1.8: oxalate-CoA ligase EC 6.2.1.9: malate-CoA ligase EC 6.2.1.10: ... threonine-tRNA ligase EC 6.1.1.4: leucine-tRNA ligase EC 6.1.1.5: isoleucine-tRNA ligase EC 6.1.1.6: lysine-tRNA ligase EC 6.1. ...
"Entrez Gene: LIG1 ligase I, DNA, ATP-dependent". Sriskanda V, Shuman S (January 1998). "Chlorella virus DNA ligase: nick ... fragments formed during discontinuous DNA synthesis on the DNA's lagging strand after DNA polymerase δ has replaced the RNA ... DNA ligase 1 is an enzyme that in humans is encoded by the LIG1 gene. DNA ligase I is the only known eukaryotic DNA ligase ... Eukaryotic DNA ligase 1 catalyzes a reaction that is chemically universal to all ligases. DNA ligase 1 utilizes adenosine ...
2005). "Glutamate cysteine ligase catalysis: dependence on ATP and modifier subunit for regulation of tissue glutathione levels ... 2006). "Knockdown of glutamate-cysteine ligase by small hairpin RNA reveals that both catalytic and modulatory subunits are ... Glutamate-cysteine ligase regulatory subunit is an enzyme that in humans is encoded by the GCLM gene. Glutamate-cysteine ligase ... "Entrez Gene: GCLM glutamate-cysteine ligase, modifier subunit". Dickinson DA, Levonen AL, Moellering DR, et al. (2005). "Human ...
ATP-hydrolysing)) ligase) HMGN1: encoding non-histone chromosomal protein HMG-14 HSPA13: encoding heat shock 70 kDa protein 13 ... RNA, ribosomal 45S cluster 4 RRP1: encoding protein ribosomal RNA processing protein 1 homolog A RRP1B: encoding protein ... encoding enzyme subunit ATP synthase-coupling factor 6, mitochondrial ATP5PO: encoding enzyme subunit ATP synthase subunit O, ... ABCG1: encoding ATP-binding cassette sub-family G member 1 ADAMTS1 encoding enzyme a disintegrin and metalloproteinase with ...
During the replication of DNA, there is a hole between the phosphates in the backbone left by DNA polymerase I. DNA ligase is ... The "bond" involves this linkage C−O−PO−2O−C. Discussion of phosphodiesters is dominated by their prevalence in DNA and RNA, ... Phosphodiesterase Phosphodiesterase inhibitor DNA replication, DNA, ATP Teichoic acid, DNase I PDE5 Nick (DNA) "Phosphodiester ... Phosphodiester bonds make up the backbones of DNA and RNA. The phosphate is attached to the 5' carbon. The 3' carbon of one ...
During lagging strand synthesis, DNA ligase I connects the Okazaki fragments, following replacement of the RNA primers with DNA ... It is known that ATP reduces activity, but promotes the release of the 3'-end label. Studies have suggested that a new model of ... When the PCNA binding site on DNA ligase I is inactive, DNA ligase I's ability to connect Okazaki fragments is severely ... Then, DNA ligase I binds to the PCNA, which is clamped to the nicks of the lagging strand, and catalyzes the formation of ...
This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with ... Min B, Pelaschier JT, Graham DE, Tumbula-Hansen D, Soll D (2002). "Transfer RNA-dependent amino acid biosynthesis: an essential ... L-glutamate The 3 substrates of this enzyme are ATP, aspartyl-tRNA(Asn), and L-glutamine, whereas its 4 products are ADP, ... The systematic name of this enzyme class is aspartyl-tRNAAsn:L-glutamine amido-ligase (ADP-forming). This enzyme participates ...
The enzyme phosphoribosylamine-glycine ligase catalyses the reaction forming an amide bond: PRA + glycine + ATP → GAR + ADP + ... and hence is a building block for DNA and RNA. The vitamins thiamine and cobalamin also contain fragments derived from PRA. It ... PPi The biosynthesis pathway next combines PRA with glycine in a process driven by ATP giving glycineamide ribonucleotide (GAR ...
In enzymology, a glycine-tRNA ligase (EC 6.1.1.14) is an enzyme that catalyzes the chemical reaction ATP + glycine + tRNAGly ... Fraser MJ (May 1963). "Glycyl-RNA synthetase of rat liver: partial purification and effects of some metal ions on its activity ... Glycine-tRNA ligase also known as glycyl-tRNA synthetase is an enzyme that in humans is encoded by the GARS1 gene. This gene ... The systematic name of this enzyme class is glycine:tRNAGly ligase (AMP-forming). Other names in common use include glycyl-tRNA ...
In enzymology, a serine-tRNA ligase (EC 6.1.1.11) is an enzyme that catalyzes the chemical reaction ATP + L-serine + tRNASer ... seryl-transfer RNA synthetase, seryl-transfer ribonucleic acid synthetase, and serine translase. This enzyme participates in ... The systematic name of this enzyme class is L-serine:tRNASer ligase (AMP-forming). Other names in common use include seryl-tRNA ... Portal: Biology v t e (EC 6.1.1, Enzymes of known structure, All stub articles, Ligase stubs). ...
... isoleucyl-transfer RNA synthetase, isoleucine-transfer RNA ligase, isoleucine-tRNA synthetase, and isoleucine translase. This ... In enzymology, an isoleucine-tRNA ligase (EC 6.1.1.5) is an enzyme that catalyzes the chemical reaction ATP + L-isoleucine + ... The systematic name of this enzyme class is L-isoleucine:tRNAIle ligase (AMP-forming). Other names in common use include ... L-isoleucyl-tRNAIle The 3 substrates of this enzyme are ATP, L-isoleucine, and tRNA(Ile), whereas its 3 products are AMP, ...
... alanyl-transfer RNA synthetase, alanyl-transfer ribonucleic acid synthetase, alanine-transfer RNA ligase, alanine transfer RNA ... In enzymology, an alanine-tRNA ligase (EC 6.1.1.7) is an enzyme that catalyzes the chemical reaction ATP + L-alanine + tRNAAla ... The systematic name of this enzyme class is L-alanine:tRNAAla ligase (AMP-forming). Other names in common use include alanyl- ... Portal: Biology v t e (EC 6.1.1, Enzymes of known structure, All stub articles, Ligase stubs). ...