... untranslated region of a newly synthesized pre-messenger RNA (mRNA) molecule in the process of gene transcription. The cleavage ... Cleavage factors are two closely associated protein complexes involved in the cleavage of the 3' ... The proteins that constitute these complexes are recruited to the cleavage site by cleavage and polyadenylation specificity ... It is composed of only two proteins: PCF11 CLP1 Hardy, J. G.; Norbury, C. J. (15 August 2016). "Cleavage factor Im (CFIm) as a ...

... to cleavage of transfer RNA; and to interference with the NF-κB pathway. Currently (as of March 2020) Ranpirnase is in clinical ... The mechanism of action of ranpirnase has been attributed to the RNA interference pathway, potentially through cleaving siRNA ... Zhao H, Ardelt B, Ardelt W, Shogen K, Darzynkiewicz Z (October 2008). "The cytotoxic ribonuclease onconase targets RNA ... protein superfamily and degrades RNA substrates with a sequence preference for uracil and guanine nucleotides. Along with ...

Donis-Keller, Helen (1979). "Site specific enzymatic cleavage of RNA". Nucleic Acids Research. 7 (1): 179-192. doi:10.1093/nar/ ... Donis-Keller, Helen (1980). "Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis". Nucleic ... Donis-Keller's graduate research established a method to do RNA sequencing. During her time at Collaborative Research, her ... Donis-Keller, Helen; Maxam, Allan M.; Gilbert, Walter (1977). "Mapping adenines, guanines, and pyrimidines in RNA". Nucleic ...

The Beta-globin co-transcriptional cleavage ribozyme (CotC ribozyme) was proposed to be an RNA enzyme known as a ribozyme. ... Dye MJ, Proudfoot NJ (2001). "Multiple transcript cleavage precedes polymerase release intermination by RNA polymerase II". ... "Autocatalytic RNA cleavage in the human beta-globin pre-mRNA promotestranscription termination". Nature. 432 (7016): 526-530. ... Non-coding RNA, Ribozymes, RNA splicing, All stub articles, Molecular and cellular biology stubs, Chemical reaction stubs, ...

doi:10.1261/rna.079397.122. ISSN 1355-8382. PMC 9745839. PMID 36113877. Altman, Sidney. "ENZYMATIC CLEAVAGE OF RNA BY RNA" (PDF ... Reed also studied the spliceosome, a multi-component RNA machine. The U2 spliceosomal RNA (U2 snRNA), is the target of the anti ... For her PhD, she worked in the laboratory of Sidney Altman and was the first to clone and sequence the RNA gene. Altman ... She showed that E7107 causes defects in the assembly of spliceosomes and stops tight binding of U2 spliceosomal RNA. She ...

RNase A is another enzyme that catalyzes the cleavage of the P-O5' bond of RNA specifically after pyrimidine residues, which is ... The mechanism would end at intron cleavage. This is regulator is thought to limit or interfere with the continuation of RNA ... Xue S, Calvin K, Li H (May 2006). "RNA recognition and cleavage by a splicing endonuclease". Science. 312 (5775): 906-910. ... After cleavage of the two sites, three fragments result: a 3'-half-tRNA molecule with a 5'-OH end, a 5'-half-tRNA molecule with ...

... small nuclear RNA (snRNA) and small nucleolar RNA (snoRNA). The basic dsRNA cleavage function of Class 1 RNase III is retained ... "Ribonuclease III mechanisms of double-stranded RNA cleavage". Wiley Interdisciplinary Reviews: RNA. 5 (1): 31-48. doi:10.1002/ ... They are ubiquitous compounds in the cell and play a major role in pathways such as RNA precursor synthesis, RNA Silencing, and ... RNAs that are produced by Dicer act as guides for a sequence of particular silencing of cognate genes through RNAi and related ...

In an additional approach, dubbed small-molecule nucleic acid profiling by cleavage applied to RNA (Ribo-SNAP) showed that ... RNA Complexation That Target the RNA: Specific Recognition of Human Immunodeficiency Virus Type 1 TAR RNA by Small Organic ... Guan, Lirui; Disney, Matthew D (2013). "Small-Molecule-Mediated Cleavage of RNA in Living Cells". Angewandte Chemie ... Additionally, HIV-1 RNA has been targeted extensively in vitro by RNA-binding small molecules. In 2007, Miller and coworkers ...

Davies, Julian E.; Schindler, Daniel G. (1977-04-01). "Specific cleavage of ribosomal RNA caused by alpha sarcin". Nucleic ... This cleavage leads to cell death by apoptosis. However, since they are extracellular proteins, they must first enter the cells ... Ribotoxins cleave RNA following a general acid-base mechanism shared by all the extracellular fungal RNases so far ... doi:10.1007/978-94-007-6728-7_16-3. ISBN 978-94-007-6728-7. Tsurugi, K.; Endo, Y. (1987-06-15). "RNA N-glycosidase activity of ...

They enable the cleavage, insertion, and deletion of bases. Trypanosomatid protists and other kinetoplastids have a novel post- ... Guide RNAs binds to the anti sense RNA sequence and regulates the RNA modification. It is observed that small interfering RNA ( ... A guide RNA (gRNA) is a piece of RNA that functions as a guide for RNA- or DNA-targeting enzymes, with which it forms complexes ... Guide RNAs are the small nucleolar RNA, these along with riboproteins perform intracellular RNA alterations such as ...

... resulting in ester cleavage of the RNA backbone. (This mechanism is also referred to as RNA cleavage.) This produces a 2',3'- ... This process is known as an auto-hydrolysis or a self-cleavage reaction. Spontaneous cleavage in an RNA molecule is much more ... RNA hydrolysis is a reaction in which a phosphodiester bond in the sugar-phosphate backbone of RNA is broken, cleaving the RNA ... When the RNA is double-stranded or involved in nucleotide base pairing, it is more stable and spontaneous cleavage is ...

The protein encoded by this gene is one subunit of the cleavage factor Im complex required for 3' RNA cleavage and ... Awasthi S, Alwine JC (2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA. 9 (11): 1400-9. doi:10.1261 ... 2005). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein ... 2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J ...

Internal guide RNA interactions interfere with Cas9-mediated cleavage. Nature Communications 7, 11750. McKenna, A., Findlay, G. ... "Ribosome profiling reveals resemblance between long non-coding RNAs and 5′ leaders of coding RNAs". Development. 140 (13): 2828 ... Schier AF (2007). "The Maternal-Zygotic Transition: Death and Birth of RNAs". Science 316 (5823): 406-7. Giraldez, A.J., ... chromatin and non-coding RNAs. Schier's more recent interest in behavior has established zebrafish as a model for sleep and ...

The granzymes bind directly to the nucleic acids DNA and RNA; this enhances their cleavage of nucleic acid binding proteins. ... June 2014). "Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding ... December 2011). "Granzyme B inhibits vaccinia virus production through proteolytic cleavage of eukaryotic initiation factor 4 ...

Removal of 3´ and 5´ extensions from DNA or RNA termini. Transcriptional mapping. Cleavage of hairpin loops. Excision of gene ... double-stranded RNA, or DNA / RNA hybrids. Mung Bean Nuclease catalyzes the specific degradation of single-stranded DNA or RNA ... An excess of the enzyme is required to degrade double-stranded DNA or RNA and DNA-RNA hybrids, and in this case, AT-rich ... Cleavage of single-basepair mismatches, as a replacement for CEL 1 Nuclease in TILLING. Unidirectional deletion of large DNA ( ...

Han BW, Wang W, Li C, Weng Z, Zamore PD (May 2015). "Noncoding RNA. piRNA-guided transposon cleavage initiates Zucchini- ... Piwi-interacting RNA (piRNA) is the largest class of small non-coding RNA molecules expressed in animal cells. piRNAs form RNA- ... piRNA has a role in RNA silencing via the formation of an RNA-induced silencing complex (RISC). piRNAs interact with piwi ... Ozata DM, Gainetdinov I, Zoch A, Phillip D, Zamore PD (2019). "PIWI-interacting RNAs: small RNAs with big functions" (PDF). ...

Thus, U1 snRNA-pre-mRNA base pairing was shown to protect pre-mRNA from polyadenylation as well as premature cleavage. This ... U1 spliceosomal RNA, U2 spliceosomal RNA, U4 spliceosomal RNA, U5 spliceosomal RNA, and U6 spliceosomal RNA. Their nomenclature ... These are small RNA molecules that play an essential role in RNA biogenesis and guide chemical modifications of ribosomal RNAs ... They have also been shown to aid in the regulation of transcription factors (7SK RNA) or RNA polymerase II (B2 RNA), and ...

"Single-stranded antisense siRNAs guide target RNA cleavage in RNAi". Cell. 110 (5): 563-74. doi:10.1016/S0092-8674(02)00908-X. ... Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K (Jan 2003). "Short-interfering-RNA-mediated gene silencing in ... This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is ... It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on ...

Zamore PD, Tuschl T, Sharp PA, Bartel DP (2000). "RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to ... RNA-induced transcriptional silencing (RITS) RNA interference Pratt AJ, MacRae IJ (2009). "The RNA-induced silencing complex: A ... RNA. 11 (5): 674-682. doi:10.1261/rna.7272305. PMC 1370754. PMID 15811921. Hutvagner, Gyorgy (2005). "Small RNA asymmetry in ... There are eight family members in human Argonautes of which only Argonaute 2 is exclusively involved in targeted RNA cleavage ...

Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T (Jul 2004). "Human Argonaute2 mediates RNA cleavage ... Argonaute Piwi siRNA RNA interference RNA-induced silencing complex RNA-dependent RNA polymerase Tabara, H.; Sarkissian, M.; ... The primary Argonaute complex recruits an RNA-dependent RNA polymerase (RdRP) to generate secondary siRNAs, triggering an ... However, RDE-1 has not been implicated in mRNA-cleavage activity. Instead, RDE-1 with mutations in the conserved DDH motif ...

End Cleavage of Target RNAs". Cell Reports. 27 (5): 1397-1408.e4. doi:10.1016/j.celrep.2019.04.008. PMC 6661904. PMID 31042468 ... RNAs are selected based on USER codes, or cis-acting elements, within their RNAs for specific levels of RNA processing; thus ... In vitro, VPg-RNA conjugates were translated with similar efficiency to m7G-capped RNAs indicating that VPg binds eIF4E and ... Its multiple roles in RNA processing require its association of RNAs through the m7G cap, and thus eIF4E can be considered a ...

Northern blot analysis also indicated this RNA undergoes specific cleavage processing. The GlmZ sRNA has been shown to ... Page for SraJ RNA at Rfam v t e (Articles with short description, Short description matches Wikidata, Non-coding RNA, All stub ... "Targeted decay of a regulatory small RNA by an adaptor protein for RNase E and counteraction by an anti-adaptor RNA". Genes & ... GlmZ (formally known as SraJ) is a small non-coding RNA (ncRNA). It is the functional product of a gene which is not translated ...

"Reversible Photocontrol of Deoxyribozyme-Catalyzed RNA Cleavage under Multiple-Turnover Conditions". Angewandte Chemie ...

"RNA methylation by Dnmt2 protects transfer RNAs against stress-induced cleavage". Genes & Development. 24 (15): 1590-5. doi: ... RNA. 14 (8): 1663-70. doi:10.1261/rna.970408. PMC 2491481. PMID 18567810. Schaefer M, Pollex T, Hanna K, Tuorto F, Meusburger M ...

They directly base-pair to target messenger RNAs (mRNAs), and can trigger cleavage of the mRNA depending on the degree of ... Circular RNA (or circRNA) is a type of single-stranded RNA which, unlike linear RNA, forms a covalently closed continuous loop ... RNAs can undergo base modification by RNA editing after transcription. RNA editing occurs mainly in Alu elements of protein- ... February 2013). "Circular RNAs are abundant, conserved, and associated with ALU repeats". RNA. 19 (2): 141-157. doi:10.1261/rna ...

... most part of a newly produced RNA and polyadenylates the end produced by this cleavage. The cleavage is catalysed by the enzyme ... The 3′ end is also where the poly(A) tail is found on polyadenylated RNAs. Messenger RNA (mRNA) is RNA that has a coding region ... But, for many long noncoding RNAs - a seemingly large group of regulatory RNAs that, for example, includes the RNA Xist, which ... The rate of deadenylation may also be regulated by RNA-binding proteins. Additionally, RNA triple helix structures and RNA ...

Prior to cleavage by exo- and endonucleases, the pre-rRNA undergoes a complex pattern of nucleoside modifications. These ... SnoRNAs are commonly referred to as guide RNAs but should not be confused with the guide RNAs that direct RNA editing in ... mainly ribosomal RNAs, transfer RNAs and small nuclear RNAs. There are two main classes of snoRNA, the C/D box snoRNAs, which ... doi:10.1261/rna.1354009. PMC 2648702. PMID 19095616. Jády BE, Kiss T (February 2001). "A small nucleolar guide RNA functions ...

"Entrez Gene: CSTF1 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa". Kleiman FE, Manley JL (September 1999). " ... Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene. This gene encodes one of ... Rüegsegger U, Beyer K, Keller W (March 1996). "Purification and characterization of human cleavage factor Im involved in the 3 ... Murthy KG, Manley JL (November 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre- ...

Okamura K, Ishizuka A, Siomi H, Siomi M (2004). "Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage ... Ahlquist P (May 2002). "RNA-dependent RNA polymerases, viruses, and RNA silencing". Science. 296 (5571): 1270-3. Bibcode: ... "Double-stranded RNA binding may be a general plant RNA viral strategy to suppress RNA silencing". Journal of Virology. 80 (12 ... Zamore PD, Tuschl T, Sharp PA, Bartel DP (March 2000). "RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at ...

The twister ribozyme is a catalytic RNA structure capable of self-cleavage. The nucleolytic activity of this ribozyme has been ... Replacements of the pro-S nonbridging oxygen of the scissile phosphate with a thiol group leads to reduced self-cleavage rates ... In contrast to in vitro selection methods, which have aided in identifying several classes of catalytic RNA motifs, the twister ... Eiler, Daniel; Wang, Jimin; Steitz, Thomas A. (2014-09-09). "Structural basis for the fast self-cleavage reaction catalyzed by ...