A correlative immunohistochemical and reverse-transcriptase polymerase chain reaction analysis". Virchows Arch. 440 (5): 461-75 ...
A correlative immunohistochemical and reverse-transcriptase polymerase chain reaction analysis". Virchows Archiv. 440 (5): 461- ...
A correlative immunohistochemical and reverse-transcriptase polymerase chain reaction analysis". Virchows Archiv. 440 (5): 461- ...
A correlative immunohistochemical and reverse-transcriptase polymerase chain reaction analysis". Virchows Archiv. 440 (5): 461- ...
These facilities use reverse transcriptase polymerase chain reaction (RT-PCR) kits. The facilities are meant to prioritize the ...
Grillo M, Margolis FL (September 1990). "Use of reverse transcriptase polymerase chain reaction to monitor expression of ... Becker-André M, Hahlbrock K (November 1989). "Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel ... RNA is first copied as complementary DNA (cDNA) by a reverse transcriptase enzyme before the resultant cDNA is sequenced. ... Fragmentation may be achieved by chemical hydrolysis, nebulisation, sonication, or reverse transcription with chain-terminating ...
Biswal M, Ratho R, Mishra B (September 2007). "Usefulness of reverse transcriptase-polymerase chain reaction for detection of ... 10: PCR technology for lyssavirus diagnosis". In Clewley JP (ed.). The Polymerase Chain Reaction (PCR) for Human Viral ... the viral polymerase will begin to synthesize new negative strands of RNA from the template of the positive-strand RNA. These ... and the viral RNA polymerase (L). To enter cells, trimeric spikes on the exterior of the membrane of the virus interact with a ...
... in gynecologic malignancies and malignant effusions detected by nested reverse transcriptase-polymerase chain reaction". ...
"Feline coronavirus quantitative reverse transcriptase polymerase chain reaction on effusion samples in cats with and without ... "Comparison of real-time reverse transcriptase polymerase chain reaction of peripheral blood mononuclear cells, serum and cell- ... "Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus ... such as by reverse transcription polymerase chain reaction (RT-PCR) is diagnostic of effusive FIP. However, that does require ...
Reverse transcriptase polymerase chain reaction (RT-PCR) methods have been developed for detection of TSV and are very ... The IQ2000TM TSV detection system, a RT-PCR method, is said to have a detection limit of 10 copies per reaction. RNA-based ... These very high rates might be due to the lack of proofreading function of the RNA-dependent RNA polymerase and have resulted ...
"Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since ... Reverse transcription converts RNA into DNA. Reverse transcription polymerase chain reaction (RT-PCR) first uses reverse ... digital polymerase chain reaction, microarray analysis, and next-generation sequencing. Polymerase chain reaction (PCR) is a ... As of 2021[update], the most common form of molecular test is the reverse transcription polymerase chain reaction (RT-PCR) test ...
... prognostic value of detection of circulating colorectal cancer cells using CGM2 reverse transcriptase-polymerase chain reaction ...
The genome is detectable by reverse transcriptase-polymerase chain reaction in the head, thorax, abdomen and wings of infected ... The genome structure is 5'UTR-L-VP2-(VP4)-VP1-VP3-RNA helicase-(VPg)-3C protease-RNA dependent RNA polymerase-3'UTR The ... a chymotrypsin-like 3C protease and an RNA-dependent RNA polymerase. VP1 is encoded between codons 486 to 880 and VP3 lies ...
2021). "Accuracy and cost description of rapid antigen test compared with reverse transcriptase-polymerase chain reaction for ... which is lower than COVID-19 polymerase chain reaction (PCR) tests' sensitivity of 88%-96%. Despite this, COVID-19 RATs remain ...
ISBN 978-0-470-84475-5. Callahan JD, et al., Use of a portable real-time reverse transcriptase-polymerase chain reaction assay ... Quantitative PCR utilizes polymerase chain reaction chemistry to amplify viral DNA or RNA to produce high enough concentrations ... "Taqman Technology and Real-Time Polymerase Chain Reaction". In Crocker, J.; Murray, P.G. (eds.). Molecular Biology in Cellular ... SYBR Green dye binds to all double-stranded DNA produced during the reaction. While SYBR Green is easy to use, its lack of ...
These tags can be appended to gene specific primers for reverse transcriptase polymerase chain reaction (RT-PCR) experiments, ... In order to overcome genomic DNA contamination in transcriptional studies, reverse template-specific polymerase chain reaction ... of using tags whose sequences are not found in the genome further improves reverse specific polymerase chain reaction ... Shuldiner, Alan R.; Ajay Nirula; Jesse Roth (1990). "RNA template-specific polymerase chain reaction (RS-PCR): a novel strategy ...
Ikeguchi M, Hirooka Y, Kaibara N (September 2003). "Quantitative reverse transcriptase polymerase chain reaction analysis for ...
"Detection and quantitation of infectious pancreatic necrosis virus by real-time reverse transcriptase-polymerase chain reaction ... Suebsing R, Jeon CH, Oh MJ, Kim JH (March 2011). "Reverse transcriptase loop-mediated isothermal amplification assay for ... Viral RNA encodes proteins VP1, VP2, VP3 and VP3a, VP4, VP5, and VP1-RNA-dependant RNA polymerase. VP1, the largest protein at ... Shao L, Zhao J, Tang Q (2021-02-25). "Non-muscle myosin heavy chain 9 is a critical factor for infectious pancreatic necrosis ...
v t e (Orphaned articles from December 2018, All orphaned articles, Reverse transcriptase inhibitors, Polymerase chain reaction ... Template-switching polymerase chain reaction (TS-PCR) is a method of reverse transcription and polymerase chain reaction (PCR) ... Zhu YY, Machleder EM, Chenchik A, Li R, Siebert PD (April 2001). "Reverse transcriptase template switching: a SMART approach ... and adds a second primer through the activity of murine leukemia virus reverse transcriptase. This permits reading full cDNA ...
2006). "A validated quantitative assay to detect occult micrometastases by reverse transcriptase-polymerase chain reaction of ... 2009). "GUCY2C reverse transcriptase PCR to stage pN0 colorectal cancer patients". Expert Rev. Mol. Diagn. 9 (8): 777-85. doi: ...
... and Application for Localization in Various Human Tissues by Real-Time Reverse Transcriptase-Polymerase Chain Reaction". Drug ... glucuronides is known to occur to nucleophilic sites on serum albumin via transacylation reactions, for example. Phenols, ...
... and Application for Localization in Various Human Tissues by Real-Time Reverse Transcriptase-Polymerase Chain Reaction". Drug ... Al-Zoughool M., Talaska, G. (2006). "4-Aminobiphenyl N-glucuronidation by liver microsomes: optimization of the reaction ...
The identification of HMPV has predominantly relied on reverse-transcriptase polymerase chain reaction (RT-PCR) technology to ... We see that proteins N, P, and L dissociate from the vRNA and bind to each other to form the polymerase complex so that the ...
They typically use reverse transcriptase polymerase chain reactions to multiply the amount of genetic material in a small ...
"Detection of human 11 beta-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and ... In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol ... It belongs to the family of short-chain dehydrogenases. It is encoded by the HSD11B1 gene. The protein encoded by this gene is ...
... a reverse transcriptase-polymerase chain reaction (RT-PCR) study". Brain Research. Molecular Brain Research. 44 (1): 92-8. doi: ... For the latter, PICK1 and PKC can displace GRIP1 to return AMPARs to the surface, reversing the effects of endocytosis and LTD ... of excitatory neurotransmission by decanoic acid in the brain contributes to the anticonvulsant effect of the medium-chain ...
"Quantitative method to determine mRNA levels by reverse transcriptase-polymerase chain reaction from leukocyte subsets purified ... In THC, CBD, and CBN, this side-chain is a pentyl (5-carbon) chain. In the most common homologue, the pentyl chain is replaced ... However, some do not follow this rule, primarily because of variation in the length of the side-chain attached to the aromatic ... Cannabinoids with the propyl side chain are named using the suffix varin and are designated THCV, CBDV, or CBNV, while those ...
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a powerful and effective method for detection of potato ... Different types of reverse transcriptase polymerases are available to suit different needs and reaction conditions. Reverse ... It could also be that the reverse transcriptase polymerase and DNA polymerase is one and the same enzyme and that the enzyme ... the RNA of the virus must first be transcribed to DNA by means of a reverse transcriptase polymerase. This polymerase ...
Splice modification can be conveniently assayed by reverse-transcriptase polymerase chain reaction (RT-PCR) and is seen as a ... Morpholinos are used as research tools for reverse genetics by knocking down gene function. This article discusses only the ...
... reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ-RT-PCR for the detection of canine distemper virus RNA in ... and other evidence suggests an intrinsic hyperresponsive reaction to vitamin D and RANK ligand is the cause.[citation needed] ...