Weasner BM, Zhu J, Kumar JP (2017). "FLPing Genes On and Off in Drosophila". Site-Specific Recombinases. Methods in Molecular ...
Unlike tyrosine recombinases, serine recombinases are highly modular, as was first hinted by biochemical studies and later ... Cre recombinase Cre-Lox recombination FLP-FRT recombination Genetic recombination Homologous recombination Recombinase-mediated ... most site-specific recombinases are grouped into one of two families: the tyrosine (Tyr) recombinase family or serine (Ser) ... recombinase family. The names stem from the conserved nucleophilic amino acid residue present in each class of recombinase ...
Akopian A, He J, Boocock MR, Stark WM (July 2003). "Chimeric recombinases with designed DNA sequence recognition". Proc. Natl. ...
This germ-line can then be crossed to another germline containing Cre-recombinase which is a viral enzyme that can recognize ... Le, Yunzheng; Sauer, Brian (2001-03-01). "Conditional gene knockout using cre recombinase". Molecular Biotechnology. 17 (3): ...
Cre recombinase proteins bind to the first and last 13 bp regions of a lox site forming a dimer. This dimer then binds to a ... A separate recombinase recognizes and binds to each of two recombination sites on two different DNA molecules or within the ... The Cre recombinase is fused to a mutant form of the oestrogen receptor, which binds the synthetic oestrogen 4-hydroxytamoxifen ... Cre, or cyclic recombinase, is one such enzyme. Site-specific recombination is, thus, the enzyme-mediated cleavage and ligation ...
a gene encoding for a site-specific recombinase: intI, belonging to the integrase family ...
Recombination in bacteria is ordinarily catalyzed by a RecA type of recombinase. These recombinases promote repair of DNA ... "Evolution of bacterial recombinase A (recA) in eukaryotes explained by addition of genomic data of key microbial lineages". ...
"Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". J. Biol. Chem. 293: 4191- ... In this way, Dmc1 is specially suited to its role as a meiosis-specific recombinase, as this activity permits it more ... Bugreev DV, Golub EI, Stasiak AZ, Stasiak A, Mazin AV (2005). "Activation of human meiosis-specific recombinase Dmc1 by Ca2+". ... During meiosis, the two recombinases, Rad51 and Dmc1, interact with single-stranded DNA to form specialized filaments that are ...
SynExo is a viral recombinase functional unit. It is thought that it may have evolved as a portable module that can function ... It thus forms part of the two-component SynExo viral recombinase functional unit. The function of this protein domain is to ... "Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases". Journal of ...
Site-specific recombinase technology Beck, B. J.; Freudenreich, Oliver; Worth, Jonathan L. (2010-01-01), Stern, Theodore A.; ... Retroviral INs are not to be confused with phage integrases (recombinases), such as λ phage integrase, as discussed in site- ... in contrast to Ser/Tyr recombinase-catalyzed reactions.[citation needed] HIV integrase is a 32 kDa protein produced from the C- ...
Using Flp recombinase, Dymecki shows that her genetic constructs can be expressed in mammalian cells to activate specific genes ... "Use of FLP recombinase in mice - Dimensions". app.dimensions.ai. Retrieved 2020-05-08. Dymecki, S. M.; Niederhuber, J. E.; ... After establishing Flp recombinase as an available genetic tool for site specific recombination in the mouse brain, Dymecki ... Nat Genet 2000-06-01 Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice. ...
Bischof J, Basler K (2008). "Recombinases and their use in gene activation, gene inactivation, and transgenesis". Methods in ... expression patterns may be achieved by combination of the three binary expression systems and the FLP/FRT or other recombinases ...
Eukaryotic recombinases that are homologues of RecA are also widespread in eukaryotic organisms. For example, in fission yeast ... "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases". Nature. 451 (7181): 1018-21. Bibcode ...
In several mouse studies, tamoxifen is used to induce the Cre recombinase. In this case, Cre recombinase is fused to a portion ... Additionally, Cre recombinase can be induced by heat when under the control of specific heat shock elements (HSEs). Nagy A ( ... The Cre recombinase will detect the unidirectional loxP sites and excise the floxed segment of DNA. The successfully edited ... Cre recombinase is then used to generate a translocation between the two DNA molecules, exchanging the genetic material from ...
These mirror images serve as substrates for two ATP-dependent recombinases, fim B and fim E. These recombinases can invert the ... The gene undergoes phase variation mediated via two recombinases and is a model example of site specific inversion. The operon ... Another level of transcriptional control in E. coli is mediated by the sensitivity of the recombinases to pH and osmolarity, ... "Comparative analysis of FimB and FimE recombinase activity". Microbiology. 153 (Pt 12): 4138-4149. doi:10.1099/mic.0.2007/ ...
To date, Flp recombinase has been utilized many times in D. melanogaster. A comparison of Flp recombinase to Cre recombinase in ... Site-specific recombinase technology Recombinase-mediated cassette exchange Cre recombinase Cre-Lox recombination Genetic ... Flp-FRT Recombinase: Mechanism of Action Video of the Utilization Flp-FRT Recombinase in D. melanoster Photoreceptor ... The authors conclude that the Flp recombinase "knock-out" tool is more effective than both RNAi and Cre recombinase for the ...
Recombinases are key enzymes that catalyse the strand transfer step during recombination. RecA, the chief recombinase found in ... In yeast and other eukaryotic organisms there are two recombinases required for repairing DSBs. The RAD51 protein is required ... hybrid genome Four-gamete test Independent assortment Recombination frequency Recombination hotspot Site-specific recombinase ...
The meiosis-specific recombinase Dmc1 and the general recombinase Rad51 coat the single-stranded DNA to form nucleoprotein ... The recombinases catalyze invasion of the opposite chromatid by the single-stranded DNA from one end of the break. Next, the 3 ... Recombinases and primases lay a foundation of nucleotides along the DNA sequence. One such particular protein complex that is ... The Holliday junction is a tetrahedral structure which can be 'pulled' by other recombinases, moving it along the four-stranded ...
The other is the ccr gene complex including genes coding for recombinases. The mec complex is divided further into five types ( ...
Tiscornia G, Tergaonkar V, Galimi F, Verma IM (May 2004). "CRE recombinase-inducible RNA interference mediated by lentiviral ...
Partial recA gene for recombinase A, strain LMG 14187T. Complete rpoA gene for RNA polymerase alpha subunit, type strain LMG ...
Likewise, site-specific recombinases also show a varied range of affinities for different target sites. The existence of ... From then on, DNA binding sites for many transcription factors, restriction enzymes and site-specific recombinases have been ... DNA binding sites also encompasses the targets of other proteins, like restriction enzymes, site-specific recombinases (see ... Gyohda A, Komano T (2000). "Purification and characterization of the R64 shufflon-specific recombinase". Journal of ...
Recombinases employed in this process are produced by archaea (RadA recombinase), by bacteria (RecA recombinase) and by ... "Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". J. Biol. Chem. 293 (11): ... A central intermediate step in this process is the interaction of multiple copies of a recombinase protein with single-stranded ... eukaryotes from yeast to humans (Rad51 and Dmc1 recombinases). Play media A ribonucleoprotein (RNP) is a complex of ribonucleic ...
A series of steps catalyzed in part by the recombinase then leads to joining of the two helices by at least one Holliday ... The recombination reaction is catalyzed by enzymes known as recombinases, such as RAD51. The first step in recombination is a ...
However, recombinases and integrases operate on DNA, which can sometimes be singled-stranded. Therefore, it is also possible ... but is predicted to generally fit into a recombinase or integrase role. Given this genetic arrangement, it is likely that xerDC ...
"Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments". The Journal of Biological ... The recombinase paralog rfs-1 is found in the round worm Caenorhabditis elegans, where it is not essential for homologous ... Among archaea the RadB and RadC recombinase paralogs are found in many organisms belonging to Euryarchaeota while a broader ... During meiosis, the two recombinases, Rad51 and Dmc1, interact with single-stranded DNA to form specialized filaments that are ...
This cassette is a 52 kilobase piece of DNA that contains mecA and two recombinase genes, ccrA and ccrB. Proper insertion of ... "Multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in Staphylococcus aureus ... the mecA complex into the host genome requires the recombinases. Researchers have isolated multiple genetic variants from ...
... s enhance the DNA strand exchange activity of RAD51 recombinase. Depletion of polyamines sensitizes cells to genotoxic ... Polyamines appear to have an evolutionarily conserved role in regulating recombinase activity. Spermidine is synthesized from ...
... and represent a most complete set of complexes in the catalytic pathways of any DDE family recombinases, transposases or ... "Transposon molecular domestication and the evolution of the RAG recombinase". Nature. 569 (7754): 79-84. doi:10.1038/s41586-019 ...