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*  Thermal cycler
Weier, HU; Gray, JW (Jul-Aug 1988). "A programmable system to perform the polymerase chain reaction". DNA (Mary Ann Liebert, ... "A handheld real time thermal cycler for bacterial pathogen detection". Biosensors and Bioelectronics. 18 (9): 1115-1123. doi: ... is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). Thermal ... The device has a thermal block with holes where tubes holding the reaction mixtures can be inserted. The cycler then raises and ...
*  Real-time polymerase chain reaction
A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a ... The acronym "RT-PCR" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all ... Bustin, SA (2000). "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays". J ... in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR ...
*  Ligand binding assay
Real-time polymerase chain reaction. The liquid phase ligand binding assay of Immunoprecipitation (IP) is a method that is used ... Mullis, K.B; Faloona, F.L. (1987). "[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction". Methods ... As a result, change in dye fluorescence can be viewed in real time with no delay using an excitatory laser and a charge-coupled ... Real-time measurements using label-free or label-based approaches have been used to analyze biomolecular interactions on ...
*  Reverse transcription polymerase chain reaction
RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike, but they are ... Reverse transcription polymerase chain reaction (RT-PCR), a variant of polymerase chain reaction (PCR), is a technique commonly ... Bustin SA (October 2000). "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction ... "Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods ...
*  PrimerDesign
Real-time polymerase chain reaction High Resolution Melt "PrimerDesign". Archived from the original on 18 July 2010. Retrieved ... a UK-based biotechnology company that designs and sells products for quantitative real-time polymerase chain reaction (qPCR). ... Dr Rob Powell and Professor Tom Brown within the University of Southampton to share their expertise in real-time PCR and DNA ... Pathogen Detection kits House-keeping Gene assays Genomic DNA Detection kits Real-time PCR reagents geNorm reference gene ...
*  Lymphogranuloma venereum
Morré SA, Spaargaren J, Fennema JS, de Vries HJ, Coutinho RA, Peña AS (August 2005). "Real-time polymerase chain reaction to ... If polymerase chain reaction (PCR) tests on infected material are positive, subsequent restriction endonuclease pattern ... Recently a fast realtime PCR (TaqMan analysis) has been developed to diagnose LGV. With this method an accurate diagnosis is ... Virulent Chlamydia Detected Largely Among Gay Men in U.S. Los Angeles Times: 11 May 2006 Michael Brown. LGV in the UK: almost ...
*  Virus quantification
ISBN 978-0-470-84475-5. Callahan JD, et al., Use of a portable real-time reverse transcriptase-polymerase chain reaction assay ... "Taqman Technology and Real-Time Polymerase Chain Reaction". In Crocker, J.; Murray, P.G. Molecular Biology in Cellular ... 10 min with a short sample preparation time. Quantitative PCR utilizes polymerase chain reaction chemistry to amplify viral DNA ... Real-time qPCR takes around 1-4 hours and can provide quantitative results containing too few viruses to be analyzed by other ...
*  Luminex Corporation
Luminex MultiCode technology, used for real-time polymerase chain reaction (PCR) and multiplexed PCR assays. Luminex ...
*  Relative fluorescence units
... in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non- ... Frequently, real-time PCR is combined with reverse transcription to quantify messenger RNA and Non-coding RNA in cells or ... "Relative quantification of mRNA levels in Jurkat T cells with RT-real time-PCR (RT-rt-PCR): new possibilities for the screening ...
*  Pediococcus damnosus
... ropy'Pediococcus damnosus strains in wine by real-time polymerase chain reaction". Journal of Applied Microbiology. 97 (5): 910 ...
*  Tendinosis
The autologous tenocytes were sorted and purified by real-time polymerase chain reaction, and amplified by flow cytometry. The ... Time required to recover from surgery is about 4 to 6 months. Studies with a rat model of fatigue-damaged tendons suggested ... Khan, K.M.; Cook, J.L.; Kannus, P.; Maffulli, N.; Bonar, S.F. (2002-03-16). "Time to abandon the "tendinitis" myth : Painful, ... Ultimate stress, echo intensity, and normalized stiffness are all their lowest during these times. The results assert that the ...
*  Bisulfite sequencing
"Conversion-specific detection of DNA methylation using real-time polymerase chain reaction (ConLight-MSP) to avoid false ... and strategies employing polymerase chain reaction (PCR) performed under non-methylation-specific conditions (Figure 3). ... Kristensen LS, Mikeska T, Krypuy M, Dobrovic A (April 2008). "Sensitive Melting Analysis after Real Time- Methylation Specific ... By first using in vitro transcription of the region of interest into RNA (by adding an RNA polymerase promoter site to the PCR ...
*  Chicken as biological research model
... quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction ... As Aristotle opened chicken eggs at various time points of incubation, he noted how the organism changed over time. Through his ... Chickens missing their bursa, an organ with an unknown function at the time, could not be induced to make antibodies. Through ... The relatively large size of the chick as a model organism allowed scientists during this time to make these significant ...
*  Ophidiomyces ophiodiicola
"Development and use of a real-time polymerase chain reaction assay for the detection of Ophidiomyces ophiodiicola in snakes". ...
*  Fusarium circinatum
It is based on the biological enrichment of a sample followed by a real-time polymerase chain reaction assay. Many countries ... detection of Fusarium circinatum in pine seed by combining an enrichment procedure with a real-time polymerase chain reaction ... Susceptible Monterey pine trees that were repeatedly exposed to the pathogen gained resistance over time under controlled ...
*  Salmonella
... can also be detected and subtyped using multiplex or real-time polymerase chain reactions (PCR) from extracted ... Choleraesuis). At the time, Smith was working as a research laboratory assistant in the Veterinary Division of the United ... Infection may remain as the latent form for a long time, and when the function of reticular endothelial cells is deteriorated, ... "Modelling Salmonella concentration throughout the pork supply chain by considering growth and survival in fluctuating ...
*  DsrA RNA
... by Real-time Polymerase Chain Reaction (PCR) Using dsrA and apsA Genes". Microbial Ecology. 54 (3): 439-451. doi:10.1007/s00248 ...
*  Claire Wainwright
"Identification of Pseudomonas Aeruginosa by a Deplux Real-time Polymerase Chain Reaction Assay Targeting the ecfX and the gyrB ... Identification of Clonal Pseudomonas aeruginosa Isolates from Patients with Cystic Fibrosis by the Use of Real-time PCR and ...
*  Cepheid Inc
The GeneXpert System is the only system to combine on-board sample preparation with real-time PCR (polymerase chain reaction) ... It was the first amplified molecular diagnostic test using real-time polymerase chain reaction (PCR) to receive this ...
*  Newborn screening
... using real-time polymerase chain reaction (qPCR). TRECs are decreased in infants affected with SCID. SCID has not been added to ... This includes date and time of birth, date and time of sample collection, the infant's weight and gestational age. The form ... At various times since 1978, DMD has been included (often as a pilot study on a small subset of the population) in newborn ... This is an issue because the newborn period is a crucial time for the parents to bond with the child, and it has been noted ...
*  Institute of Animal Biotechnology
In 2005, the laboratory received an automatic DNA analyzer, real-time polymerase chain reaction (PCR) and a new camera for gel ...
*  Quantitative PCR instrument
"Application of Real-time Polymerase Chain Reaction (RT-PCR)", The Journal of American Science, 2 (3): 1-15 Raghavan, V.; ... Available real-time PCR cyclers Features of Real-time PCR Platforms and qPCR Machines. ... "Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System". ... Also sometimes called "real-time PCR instrument". Higuchi, R.; Dollinger, G.; Walsh, P.S.; Griffith, R. (1992), "Simultaneous ...
*  Oncogenomics
The characterization of mtDNA via real-time polymerase chain reaction assays shows the presence of quantitative alteration of ... 2005). "Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase". Nature. 434 (7035): 913- ... "Somatic mutation hotspots correlate with DNA polymerase eta error spectrum". Nature Immunology. 2 (6): 530-536. doi:10.1038/ ...
*  KPCR
United States Real-time polymerase chain reaction. ...
*  Complement component 4
study, they utilized the real-time polymerase chain reaction (rt-PCR) as an assay to determine the copy number variance (CNV) ... "Sensitive and specific real-time polymerase chain reaction assays to accurately determine copy number variations (CNVs) of ... Among them, the β-chain expressed of five, as the α-chain and γ-chain produced 18 and one, respectively. These polymorphisms ... which then undergoes proteolytic cleavage into three chains (in order of how they are chained, β-α-γ). The β-chain consists of ...
*  Enzyme inhibitor
... this reaction are investigated by incubating the enzyme with inhibitor and assaying the amount of activity remaining over time ... This is a potent enzyme inhibitor, in this case preventing the RNA polymerase II enzyme from transcribing DNA. The algal toxin ... Both these enzymes are needed for plants to make branched-chain amino acids. Many other enzymes are inhibited by herbicides, ... Competitive inhibitors are often similar in structure to the real substrate (see examples below). In uncompetitive inhibition, ...
*  Clostridium difficile infection
Testing of stool samples by real-time polymerase chain reaction is able to pick up the disease about 90% of the time and when ... "Diagnostic accuracy of real-time polymerase chain reaction in detection of Clostridium difficile in the stool samples of ... Further, reactions to medication may be severe: CDI infections were the most common contributor to adverse drug events seen in ... During that time, the review also noted 375 instances of CDIs in patients. In October 2007, Maidstone and Tunbridge Wells NHS ...