*Ligand binding assay

"Calculations and Instrumentation used for Radioligand Binding Assays". Assay Guidance Manual: 1-21. Davenport, Anthony P. (2005 ... binding-assay.com/binding-assay-types/solid-phase-assays/on-bead-assays Moss, ed. by Tom (2001). "Filter-Binding Assays". DNA- ... Ligands are radiolabeled using either 3H or 125I, and released into the assay. Since only the radioligands that directly bind ... Ligand binding assays (LBA) is an assay, or an analytic procedure, whose procedure or method relies on the binding of ligand ...

*List of MeSH codes (E01)

... radioligand assay MeSH E01.370.374.750 --- thyroid function tests MeSH E01.370.374.750.100 --- basal metabolism MeSH E01.370. ... radioligand assay MeSH E01.370.384.730 --- radionuclide imaging MeSH E01.370.384.730.050 --- absorptiometry, photon MeSH ... local lymph node assay MeSH E01.370.750.600 --- passive cutaneous anaphylaxis MeSH E01.370.750.610 --- patch tests MeSH E01.370 ... local lymph node assay MeSH E01.450.495.750.600 --- passive cutaneous anaphylaxis MeSH E01.450.495.750.610 --- patch tests MeSH ...

*Folate targeting

"Folate receptor expression in carcinomas and normal tissues determined by a quantitative radioligand binding assay". Anal. ... These include an invasive tissue-based immunohistochemical assay, and a non-invasive radiodiagnostic approach. The latter ...

*Thermal shift assay

One of the methods researchers used to guide these alterations was radioligand binding thermostability assay. The assay is ... Biochemical assays require a catalytic activity of the protein in question as well as a specific assay. Circular dichroism and ... The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the ... The radioligand concentration is high enough to saturate the protein. Denatured protein is unable to bind the radioligand and ...

*3-MeO-PCP

However, radioligand binding assays with human proteins have shown that, contrary to common belief, the drug also does not ...

*Alazocine

... for instance in radioligand binding assays. Taken together, (-)-alazocine is a selective partial agonist of the κ-opioid ...

*Vintafolide

"Folate receptor expression in carcinomas and normal tissues determined by a quantitative radioligand binding assay". 338 (2). ...

*Clinical neurochemistry

One assay to determine the extent at which a ligand binds to its receptor is the radioligand binding assay (RBA), in which ... While the RBA assay assumes that the tissue prepared has just one molecular target per ligand, in actuality this may not be the ... For example, serotonin binds to many diverse serotonin receptors which makes the RIA assay quite difficult to interpret. ...

*BindingDB

... and radioligand and competition assays. BindingDB includes data extracted from the scientific literature by the BindingDB ...

*Oncostatin M

... displays similar binding affinity toward cells in radio ligand binding assays. Thus post translational processing may play a ... Scatchard analysis of radio ligand binding data from 125I-OSM binding to a variety of OSM responsive cell lines produced ... As a result, several investigators utilised human OSM in mouse assays and thus any conclusion drawn from the results of these ... Pro-OSM although an order of magnitude less efficacious in growth inhibition assays, ...

*A-423,579

... structure activity relationships of analogues of A-331440 combining radioligand receptor binding assays and micronucleus assays ...

*IC50

In this type of assay, a single concentration of radioligand (usually an agonist) is used in every assay tube. The ligand is ... Assay Guidance // Assay Guidance Manual // Assay Operations for SAR Support Glaxo Wellcome and Science - Global Cheng Y, ... The level of specific binding of the radioligand is then determined in the presence of a range of concentrations of other ... Ki is the inhibition constant for a drug; the concentration of competing ligand in a competition assay which would occupy 50% ...

*Butyrfentanyl

"Fentanyl receptor assay. II. Utilization of a radioreceptor assay for the analysis of fentanyl analogs in urine". J Anal ... fentanyl as the radioligand. The Ki value for butyrfentanyl was Ki=32 ± 4.1 nM. Comparing to fentanyl's Ki (Ki=1.06 ± 0.15 nM ... The opioid receptor affinity of fentanyl and its analogs was determined from their inhibitory potency in a binding assay with [ ... and Depressant Drug Indices Utilization of a radioreceptor assay for the analysis of fentanyl analogs in urine Mario Enrique ...

*Radioligand

This assay is commonly used to calculate binding constant of molecules to receptors. The transport of the radioligand is ... Examples of radioligands include: 11C-WAY-100635 for the 5-HT1A receptor N(1)-([11C]-methyl)-2-Br-LSD ([11C]-MBL) for 5-HT2 ... A radioligand is a radioactive biochemical substance (in particular, a ligand that is radiolabeled) that is used for diagnosis ... Radioligands are credited with making possible the study of biomolecular behaviour, a previously mysterious area of research ...

*Cyprodime

... characterization by in vitro radioligand and [35S]GTPgammaS binding assays. European Journal of Pharmacology. 1999 Oct 27;383(2 ...

*Ketanserin

With tritium (3H) radioactively labeled ketanserin is used as a radioligand for serotonin 5-HT2 receptors, e.g. in receptor ... binding assays and autoradiography. This radio-labeling has enabled the study of serotonin 5-HT2A receptor distribution in the ...

*Assay

Testing for Illegal Drugs Radioligand binding assay When multiple assays measure the same target their results and utility may ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ... Chemotaxis assay Secretion assays Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity ... MTT assay Cell Counting Kit-8 (WST-8 based cell viability assay) SRB (Sulforhodamine B) assay CellTiter-Glo® Luminescent Cell ...

*PET radiotracer

This assay is commonly used to calculate binding constant of molecules to receptors. Due to the probable injuries of PET- ... In in vivo systems it is often used to quantify the binding of a test molecule to the binding site of radioligand. The higher ... PET radiotracer or PET-ligand is a type of radioligand that is used for the diagnostic purposes via positron emission ... Medicinal radiocompounds List of PET radiotracers Positron emission tomography Medicinal radiochemistry Radioligand Wadsak ...

*David E. Nichols

His lab also first developed [125I]-(R)-DOI as a radioligand. Nichols is one of the few people who has published legitimate ... He has done extensive work using the rat model assay of drug discrimination in characterizing hallucinogenic drugs. Although ...

*PSB-10

The affinity towards adenosine A3 subtype was measured against the radioligand PSB-11. Ozola V, Thorand M, Diekmann M, Qurishi ... GTPγS binding assay using hA3-CHO-cells indicated that PSB-10 acts as an inverse agonist (IC50 = 4 nM). It has been shown to ... a novel high-affinity antagonist radioligand for human A(3) adenosine receptors". Bioorg Med Chem Lett. 12 (3): 501-3. doi: ...

*Trimipramine

2011) assayed desmethyltrimipramine, 2-hydroxytrimipramine, and trimipramine-N-oxide in addition to trimipramine and found that ... study were due to methodological differences, in particular the use of radioligand binding in isolated membranes (KD) to study ...

*Receptor antagonist

In functional antagonist assays, a dose-response curve measures the effect of the ability of a range of concentrations of ... of an antagonist can be determined experimentally using Schild regression or for competitive antagonists in radioligand binding ... Altering the amount of antagonist used in the assay can alter the dose ratio. In Schild regression, a plot is made of the log ( ... In functional assays of non-competitive antagonists, depression of the maximal response of agonist dose-response curves, and in ...

*VER-3323

"Development of homogeneous high-affinity agonist binding assays for 5-HT2 receptor subtypes". ASSAY and Drug Development ... "Pharmacological characterisation of the agonist radioligand binding site of 5-HT(2A), 5-HT(2B) and 5-HT(2C) receptors". Naunyn- ...

*Methods to investigate protein-protein interactions

Proximity ligation assay (PLA) in situ is an immunohistochemical method utilizing so called PLA probes for detection of ... Björke, Henrik; Andersson, Karl (2006-01-01). "Measuring the affinity of a radioligand with its receptor using a rotating cell ... Pull-down assays are a common variation of immunoprecipitation and immunoelectrophoresis and are used identically, although ... ISBN 978-3-639-31160-0. [page needed] Wienken CJ, Baaske P, Rothbauer U, Braun D, Duhr S (2010). "Protein-binding assays in ...

*Hydra (genus)

Recently, an assay for measuring the feeding response in hydra has been developed. In this method, the linear two-dimensional ... Pharmacological and radioligand binding studies. Comp. Biochem. Phys. C. 87, 321-324 (1987) Kulkarni RS and Galande S, 2014, ...

*Κ-opioid receptor

... radioligand binding and behavioural studies in two rat models of neuropathic pain". Pain. 132 (3): 289-300. doi:10.1016/j.pain. ... on a neuroendocrine biomarker assay in nonhuman primates with high kappa-receptor homology to humans". The Journal of ...