The Flyer Gene Choice provides competent cells for cloning applications. GenClone provides cell culture media, sera (FBS), ... proprietary products for protein biology research. 2017 - Launched GenClone product line, a full line of cell culture media ... 5867964) GenClone, High-performance cell culture media products (Reg. #: 5322208 ) Gene Choice, Competent cells for cloning ( ... Prometheus provides protein separation/purification resins and Western blotting reagents and consumables. SECadex TITAN ...
Strachan GD, Ostrow LA, Jordan-Sciutto KL (2005). "Expression of the fetal Alz-50 clone 1 protein induces apoptotic cell death ... Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA- ... 2000). "Fetal Alz-50 clone 1 (FAC1) protein interacts with the Myc-associated zinc finger protein (ZF87/MAZ) and alters its ... "Fetal Alz-50 clone 1 (FAC1) protein interacts with the Myc-associated zinc finger protein (ZF87/MAZ) and alters its ...
"A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains". Nature. 308 (5955): ... UMich Orientation of Proteins in Membranes protein/pdbid-2hac - Zeta-zeta dimer of T-cell receptor T-Cell+Receptor at the U.S. ... The T-cell receptor (TCR) is a protein complex found on the surface of T cells, or T lymphocytes, that is responsible for ... T cells expressing this receptor are referred to as α:β (or αβ) T cells, though a minority of T cells express an alternate ...
... cloning and expression; cell culture; PRR ligants; antibodies and ELIZAs; proteins and peptides; genes and promoters. Invivogen ... cell culture, cloning and gene expression. Current products are classified as: cell lines; inhibitors; vaccine adjuvants; ... "Tools for Cell Culture, Immunology and Molecular Biology". InvivoGen. 2016-09-12. Retrieved 2022-10-31. "Tools for Cell Culture ... InvivoGen is a provider of Toll-like receptor related products (mainly ligands and engineered mammalian cell lines), selection ...
... cells derived from bone marrow). Monoclonal antibodies are man-made proteins produced by cloning a singular cell. This method ... Next, a cell scraper is used to separate the cells, and the cells are analyzed to see if they contain the protein commonly ... This protein ensures efficient cell to cell propagation (producing seeds). The fourth ORF codes for capsid protein (the hard ... The fifth ORF codes for nucleic-acid binding protein. This is the protein that helps the DNA or RNA connect with amino acids. ...
ACA2 was originally cloned from HeLa cells by association with GAR1 protein. It has the predicted hairpin-hinge-hairpin-tail ... This gene encodes a predicted protein referred to as FLJ20436. Both variants of ACA2 have the same two predicted target sites ( ...
cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog". J. Biol. Chem. 267 (13): 9101-6. ... Probable G-protein coupled receptor 135 is a protein that in humans is encoded by the GPR135 gene. GRCh38: Ensembl release 89: ... 2003). "The G protein-coupled receptor repertoires of human and mouse". Proc. Natl. Acad. Sci. U.S.A. 100 (8): 4903-8. Bibcode: ... v t e (Articles with short description, Short description matches Wikidata, Genes on human chromosome 14, G protein-coupled ...
... "cDNA cloning and gene mapping of a candidate human cell cycle checkpoint protein". Proceedings of the National Academy of ... Serine/threonine-protein kinase ATR, also known as ataxia telangiectasia and Rad3-related protein (ATR) or FRAP-related protein ... November 2016). "Activation of the ATR kinase by the RPA-binding protein ETAA1" (PDF). Nature Cell Biology. 18 (11): 1196-1207 ... These tumor cells rely on pathways like ATR to reduce replicative stress within the cancerous cells that are uncontrollably ...
cDNA cloning and expression of the enzyme in COS 1 cells". Eur. J. Biochem. 246 (3): 681-9. doi:10.1111/j.1432-1033.1997.t01-1- ... This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes ... Bieberich E, Bause E (1995). "Man9-mannosidase from human kidney is expressed in COS cells as a Golgi-resident type II ... Bause E, Bieberich E, Rolfs A, Volker C, Schmidt B (Dec 1993). "Molecular cloning and primary structure of Man9-mannosidase ...
"A cDNA clone expressed in natural killer and T cells that likely encodes a secreted protein". The Journal of Experimental ... Its expression is restricted to cytotoxic immune cells such as cytotoxic T cells, NK cells, NKT cells and γδ T cells. Orthologs ... such as NK cells, cytotoxic T cells, helper T cells, and in higher concentrations, immature dendritic cells. The 9 kDa form ... Granulysin is expressed in killer cells, such as cytotoxic T cells and Natural Killer (NK) cells, which hold the cytotoxic ...
... "cDNA cloning of a novel cell adhesion protein expressed in human squamous carcinoma cells". Biochemical and Biophysical ... The NDUFB7 protein weighs 16.4 kDa and is composed of 137 amino acids. NDUFB7 is a subunit of the enzyme NADH dehydrogenase ( ... The protein encoded by this gene is an accessory subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I) that is ... This protein complex has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the ...
Since then, CHO cells have been a cell line of choice because of their rapid growth in suspension culture and high protein ... Cell. 33 (2): 405-412. doi:10.1016/0092-8674(83)90422-1. PMID 6305508. Wurm, Florian; Wurm, Maria (2017). "Cloning of CHO cells ... CHO cells have played a significant role in the production of recombinant protein therapeutics since, with CHO cells producing ... CHO cells are the most common mammalian cell line used for mass production of therapeutic proteins. They can produce ...
This protein, like other Connexin proteins, forms connections between cells known as gap junctions. Connexin 37 can be found in ... "Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping". The ... Gap junction alpha-4 protein, also known as Connexin-37 or Cx37, is a protein that in humans is encoded by the GJA4 gene. ... an endothelial cell gap junction protein". The Journal of Clinical Investigation. 91 (3): 997-1004. doi:10.1172/JCI116321. PMC ...
TRX encoding a human T-cell lymphotrophic virus type-I Tax1 binding protein". Biochimica et Biophysica Acta (BBA) - Gene ... Mireskandari A, Reid RL, Kashanchi F, Dittmer J, Li WB, Brady JN (April 1996). "Isolation of a cDNA clone, ... Protein VAC14 homolog, also known as ArPIKfyve (Associated Regulator of PIKfyve), is a protein that in humans is encoded by the ... The ArPIKfyve protein scaffolds a trimolecular complex to tightly regulate the level of PtdIns(3,5)P2. Other components of this ...
Fukudome K, Esmon CT (Nov 1994). "Identification, cloning, and regulation of a novel endothelial cell protein C/activated ... 2001). "Identification of the protein C/activated protein C binding sites on the endothelial cell protein C receptor. ... "The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in ... "Mechanisms by which soluble endothelial cell protein C receptor modulates protein C and activated protein C function". J. Biol ...
Following PCR, the genes are cloned into a plasmid and introduced to competent cell systems. These cells are then screened for ... This knowledge of existing protein structure assists with the prediction of new protein structures. Methods for protein ... Cell free display systems have been developed to exploit in vitro protein translation or cell free translation. These methods ... "Stabilization of a Protein Nanocage through the Plugging of a Protein-Protein Interfacial Water Pocket", Biochemistry, 50 (19 ...
"Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins". The Journal of ... Blockade/knockdown of RAGE resulted in impaired cell adhesion, and increased cell proliferation and migration A number of small ... HMGB1 is an intracellular DNA-binding protein important in chromatin remodeling which can be released by necrotic cells ... molecular relations to functional changes in pulmonary cell types". American Journal of Respiratory Cell and Molecular Biology ...
Various glycoside hydrolases have also been cloned from S. pneumoniae to help analysis of cell glycosylation.[citation needed] ... Through engineering of surface molecules from this bacterium, proteins can be irreversibly linked using the sortase enzyme or ... Invasins, such as pneumolysin, an antiphagocytic capsule, various adhesins, and immunogenic cell wall components are all major ... Atromentin and leucomelone possess antibacterial activity, inhibiting the enzyme enoyl-acyl carrier protein reductase, ( ...
... and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase". The Journal of ... Wo YY, McCormack AL, Shabanowitz J, Hunt DF, Davis JP, Mitchell GL, Van Etten RL (May 1992). "Sequencing, cloning, ... It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein ... The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. ...
... however hematopoietic and neuronal cells express a higher number of PTPs in comparison to other cell types. T cells and B cells ... Wo YY, Shabanowitz J, Hunt DF, Davis JP, Mitchell GL, Van Etten RL, McCormack AL (2023). "Sequencing, cloning, and expression ... 2020). "Protein tyrosine phosphatases in the human genome". Cell. 117 (6): 699-711. doi:10.1016/j.cell.2004.05.018. PMID ... a protein]-tyrosine phosphate + H2O = [a protein]-tyrosine + phosphate Protein tyrosine (pTyr) phosphorylation is a common post ...
The dog was cloned using viral transfection of fibroblasts cells which expresses the red fluorescent gene. The nucleus of the ... A cloned beagle, Ruppy and four other beagles produce a fluorescent protein that glows red upon excitation with ultraviolet ... leading to generation of dog oocytes expressing the red fluorescent protein. These cloned embryos were then implanted into the ... Cloned dogs, Genetically modified organisms, All stub articles, Dog stubs). ...
The protein encoded by this gene functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen ... Masuhara M, Nagao K, Nishikawa M, Sasaki M, Yoshimura A, Osawa M (Mar 2000). "Molecular cloning of murine STAP-1, the stem-cell ... A mouse ortholog, stem cell adaptor protein 1, shares 83% identity with its human counterpart. STAP1 has been shown to interact ... Signal-transducing adaptor protein 1 is a protein that in humans is encoded by the STAP1 gene. ...
... did not cause an alteration of cell proliferation and led to significant decreases in protein and DNA contents in cloned normal ... Protein transport protein Sec16B also known as regucalcin gene promoter region-related protein p117 (RGPR-p117) and leucine ... RGPR-p117 induces the decrease in protein and DNA contents in cloned normal rat kidney proximal tubular epithelial NRK52E cells ... The complete sequences of 60 new cDNA clones from brain which code for large proteins". DNA Research. 8 (4): 179-87. doi: ...
Chen J, Liu L, Pohajdak B (Dec 1995). "Cloning a cDNA from human NK/T cells which codes for a protein with high proline content ... PNRC1 protein, human at the U.S. National Library of Medicine Medical Subject Headings (MeSH) NURSA C108 v t e (Articles with ... Proline-rich nuclear receptor coactivator 1 is a protein that, in humans, is encoded by the PNRC1 gene. PNRC1 functions as a ... a proline-rich nuclear receptor coregulatory protein that modulates transcriptional activation of multiple nuclear receptors ...
Hlx belongs to the class of homeobox transcription factors, initially cloned from a B-lymphocyte cell line. Targeted knockout ... Homeobox Protein HB24 is a protein that in humans is encoded by the HLX gene. ... HLX+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Deguchi Y, Moroney JF, Wilson GL, Fox CH, Winter HS, Kehrl JH (April 1991). "Cloning of a human homeobox gene that resembles a ...
Reprogramming can be accomplished by nuclear cloning, cell fusion with pluripotent cells, or expression of pluripotency factors ... For example, a mutation of the Polycomb protein EED leads to a lack of Xist RNA coating on the Xi and X reactivation in ... of embryonic stem cells. Cell hybrid experiments fusing somatic cells and stem cells in vitro resulted in reactivation of the ... In non-cell-autonomous X-linked disorders, such as hemophilia A, the healthy cells can compensate for the diseased cells. In ...
Tekur S, Pawlak A, Guellaen G, Hecht NB (Jul 1999). "Contrin, the human homologue of a germ-cell Y-box-binding protein: cloning ... "Mammalian male and female germ cells express a germ cell-specific Y-Box protein, MSY2". Biol Reprod. 59 (5): 1266-74. doi: ... Y-box-binding protein 2 is a protein that in humans is encoded by the YBX2 gene. RNA polymerase II Transcription (biology) ... 2006). "Expression and cellular localization of dbpC/Contrin in germ cell tumor cell lines". Biochim. Biophys. Acta. 1759 (1-2 ...
... and role in autocrine cell growth of cell surface retention sequence binding protein-1". The Journal of Biological Chemistry. ... Huang SS, Tang FM, Huang YH, Liu IH, Hsu SC, Chen ST, Huang JS (October 2003). "Cloning, expression, characterization, ... LYVE-1 is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, ... while in the thoracal dura the cells were mainly round in morphology. The cells in brain dura were associated with collagen ...
"Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains". The ... It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. ... it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells ... Because CD20 remains present on the cells of most B-cell neoplasms, and is absent on otherwise similar appearing T-cell ...
... and characterization of a molecular cDNA clone of a human mRNA from interferon-treated cells encoding nucleolar protein B23, ... "Spatial association of HIV-1 tat protein and the nucleolar transport protein B23 in stably transfected Jurkat T-cells". ... Hale TK, Mansfield BC (December 1989). "Nucleotide sequence of a cDNA clone representing a third allele of human protein B23". ... "The roles of nucleolar structure and function in the subcellular location of the HIV-1 Rev protein". Journal of Cell Science. ...