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*  Directed evolution
Applications: Protein engineering Enzyme engineering Protein design Expanded genetic code Mutagenesis: Random mutagenesis ... As a protein engineering tool, DE has been most successful in three areas: Improving protein stability for biotechnological use ... Protein engineering. 12 (1): 47-53. doi:10.1093/protein/12.1.47. PMID 10065710. Hawkins, RE; Russell, SJ; Winter, G (5 August ... is a method used in protein engineering that mimics the process of natural selection to evolve proteins or nucleic acids toward ...
*  DNA shuffling
... protein engineering) RACHITT Homologous recombination Zhao, Huimin; Arnold, Frances H. (1997). "Optimization of DNA shuffling ...
*  Lori Ann Setton
www.pratt.duke.edu (2001). "Duke Engineering Faculty Awards". Duke University School of Engineering. Retrieved 2013-05-23. www. ... "Static compression induces zonal-specific changes in gene expression for extracellular matrix and cytoskeletal proteins in ... She received an M.S. degree and Ph.D degree in Mechanical Engineering/Biomechanics in 1988 and 1993, respectively. She joined ... She is currently the Department Chair as well as the Lucy and Stanley Lopata Distinguished Professor of Biomedical Engineering ...
*  Christopher Voigt
Engineering Synthetic Biology: Programming Living Bacteria Decoding Synthetic Biology Engineering Biology Brogan, Jacob (3 ... Levskaya A, Weiner OD, Lim WA, Voigt CA (2009). "Spatiotemporal control of cell signalling using a light-switchable protein ... Voigt is the Daniel I.C. Wang Professor of Advanced Biotechnology in the Department of Biological Engineering at Massachusetts ... PMC 3345007 . Widmaier DM, Tullman-Ercek D, Mirsky EA, Hill R, Govindarajan S, Minshull J, Voigt CA (2009). "Engineering the ...
*  Tuan Vo-Dinh
Protein Nanotechnology, Humana Press, New York, (in press). "Top 100 living geniuses". Telegraph.co.uk. 2007-10-31. Retrieved ... Goodson Professor of Biomedical Engineering, Professor of Chemistry, and Director of the Fitzpatrick Institute for Photonics of ...
*  Fed-batch culture
Bioprocess and Biosystems Engineering 2002, 24:273-279. Christopher J. Hewitt, Alvin W. Nienow: The scale-up of microbial batch ... Production of heterologous proteins by fed-batch cultures of recombinant microorganisms have been extensively studied. The ... Eds). Recombinant Protein Production with prokaryotic and eukaryotic cells. A comparative view on host physiology. 2001, Kluwer ... O Mendoza-Vega, J. Sabatie, S. W. Brown: Industrial-Production of Heterologous Proteins by Fed-Batch Cultures of the Yeast ...
*  Jonathan Rothberg
The Chediak-Higashi protein interacts with SNARE complex and signal transduction proteins. Molecular Medicine 2002; Vol. 8, 56- ... Rothberg is a member of the National Academy of Engineering, the Connecticut Academy of Medical Science and Engineering, and is ... Rothberg earned a BS in Chemical Engineering with an option in Biomedical Engineering from Carnegie Mellon University in 1985. ... A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. [Cover paper] Nature Feb 10, 2000; Vol. ...
*  Protein engineering
... is the process of developing useful or valuable proteins. It is a young discipline, with much research ... Computation-aided design has also been used to engineer complex properties of a highly ordered nano-protein assembly. A protein ... Directed evolution will likely not be replaced as the method of choice for protein engineering, although computational protein ... There are two general strategies for protein engineering: rational protein design and directed evolution. These methods are not ...
*  Centre for Protein Engineering
... stability and activity of proteins and engineering of antibodies. Centre for Protein Engineering was established in 1990 as one ... of how proteins relate to each other and how their structures and functions evolved. The MRC Centre for Protein Engineering ... The MRC Centre for Protein Engineering (or CPE) was a pioneering research unit in Cambridge, England, with a main focus on the ... It was formed around the research of two prominent scientists who invented protein engineering, Sir Alan Fersht and Sir Greg ...
*  Structure-based combinatorial protein engineering
Enzymology Expanded genetic code Gene synthesis Genome Nucleic acid analogues Protein design Protein engineering Protein ... Structure-based combinatorial protein engineering (SCOPE) is a synthetic biology technique for creating gene libraries ( ... "Structure-based combinatorial protein engineering (SCOPE)". Journal of Molecular Biology. 321 (4): 677-91. doi:10.1016/S0022- ... although the technique is generally applicable for the creation of engineered proteins with commercially desirable properties. ...
*  PAFAH1B2
Protein Engineering. 14 (7): 513-9. doi:10.1093/protein/14.7.513. PMID 11522926. Anderson NL, Polanski M, Pieper R, Gatlin T, ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ... as a prothrombotic gene in a protein C-deficient kindred and population-based case-control sample". Thrombosis and Haemostasis ...
*  Ben G. Davis
... protein engineering; drug delivery; molecular biology; structural biology; cell biology; glycobiology; molecular imaging and in ... Research in the Davis laboratory has been funded by the Engineering and Physical Sciences Research Council (EPSRC), the ... exploring protein chemistry and biocatalysis.In 1998 he returned to the United Kingdom to take up a lectureship at Durham ... with an emphasis on carbohydrates and proteins. In particular, the group's interests encompass synthesis and methodology; ...
*  DNA nanotechnology
Protein Engineering. 1 (4): 295-300. doi:10.1093/protein/1.4.295. PMID 3508280. Nanoarchitecture: Xiao, Shoujun; Liu, Furong; ... Nucleic acid structures are less versatile than proteins in their function because of proteins' increased ability to fold into ... This property is absent in other materials used in nanotechnology, including proteins, for which protein design is very ... A non-covalent hosting scheme using Dervan polyamides on a DX array was used to arrange streptavidin proteins in a specific ...
*  Molecular models of DNA
Protein Engineering. 1 (4): 295-300. doi:10.1093/protein/1.4.295. ISSN 0269-2139. PMID 3508280. Link Rothemund, Paul W. K.; ... NMR of Proteins and Nucleic Acids., New York, Brisbane,Chicester, Toronto, Singapore: J. Wiley & Sons. 1986., 292 pages. Hallin ... In eukaryotes this is carried by spool-like proteins named histones, around which DNA winds. It is the further compaction of ... The proton exchange rates in DNA and attached proteins may vary from picosecond to nanosecond, minutes or years, depending on ...
*  Cathepsin S
Protein Engineering. 11 (11): 1007-13. doi:10.1093/protein/11.11.1007. PMID 9876921. Söderström M, Salminen H, Glumoff V, ... Cathepsin S is a protein that in humans is encoded by the CTSS gene. Transcript variants utilizing alternative polyadenylation ... While lysosomal proteases terminally degrade proteins in lysosomes, cathepsin S has own distinctive physiological role. This ... proteins. Cathepsin S may be considered the most potent elastase known. The list of proposed cathepsin S substrates includes ...
*  DEPDC1B
D_R : G. Dele`age and B. Roux, An algorithm for secondary structure prediction based on class prediction, Protein Engineering, ... indicates the possible interaction of the protein with a SUMO protein, enabling or inhibiting interaction with other proteins. ... DEP Domain Containing Protein 1B also known as XTP1, XTP8,HBV XAg-Transactivated Protein 8, [formerly referred to as BRCC3] is ... The DEP domain is primarily found in proteins involved in G-protein signalling pathways and regulation of GTPase. As well, ...
*  Thermostability
Protein engineering can be used to enhance the thermostability of proteins. A number of site-directed and random mutagenesis ... Rational protein engineering for increasing protein thermostability includes mutations which truncate loops, increase salt ... Asial, I; Cheng, Y. X.; Engman, H; Dollhopf, M; Wu, B; Nordlund, P; Cornvik, T (2013). "Engineering protein thermostability ... Lehmann, M; Pasamontes, L; Lassen, S. F.; Wyss, M (2000). "The consensus concept for thermostability engineering of proteins". ...
*  Walker motifs
... in nucleotide-binding and other proteins". Protein Engineering. 15 (10): 783-798. doi:10.1093/protein/15.10.783. PMID 12468712 ... These include ATP synthase (α and β subunits), myosin, transducin, helicases, kinases, AAA proteins, G-proteins, RecA, protein ... The Walker A motif is best known for its presence in ATP- and GTP-binding proteins, and is also found in a variety of proteins ... Activation loop Autophosphorylation Ca2+/calmodulin-dependent protein kinase Cell signaling Cyclin-dependent kinase G protein- ...
*  Douglas Youvan
Protein engineering. 6 (3): 327-31. doi:10.1093/protein/6.3.327. PMID 8506267. Yang, MM; Coleman, WJ; Youvan, DC (1990). ME, ... ISBN 3-540-53420-2. Füllen, G; Youvan, DC (1994). "Genetic Algorithms and Recursive Ensemble Mutagenesis in Protein Engineering ... Arkin, AP; Youvan, DC (1992). "An algorithm for protein engineering: simulations of recursive ensemble mutagenesis". ... Youvan published the nucleotide and deduced protein sequence for the photosynthetic reaction center - the proteins that convert ...
*  Alphabody
Protein Engineering. 11 (11): 1051-1055. doi:10.1093/protein/11.11.1051. "Complix selects first Alphabody™ development ... Alphabodies, also known as Cell-Penetrating Alphabodies or CPAB for short, are small 10 kDa proteins engineered to bind to a ... allowing them to modulate intracellular protein-to-protein interactions and induce apoptosis in cancer cells. Complix expects ... Alphabodies are developed as scaffolds with a set of amino acid residues that can be modified to bind protein targets, while ...
*  PAFAH1B3
Protein Engineering. 14 (7): 513-9. doi:10.1093/protein/14.7.513. PMID 11522926. Saito Y, Doi K, Yamagishi N, Ishihara K, ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ...
*  Alpha-Bungarotoxin
Protein engineering. 1 (1): 37-46. doi:10.1093/protein/1.1.37. PMID 3507686. Tu, A. T.; Hong, B. S. (1971). "Purification and ... Changeux, J.; Kasai, M.; Lee, C. (1970). "Use of a snake venom toxin to characterize the cholinergic receptor protein". ... Levitt, M.; Sander, C.; Stern, P. S. (1985). "Protein normal-mode dynamics: Trypsin inhibitor, crambin, ribonuclease and ... a neurotoxic protein that is known to bind competitively and in a relatively irreversible manner to the nicotinic acetylcholine ...
*  Loop modeling
Protein engineering. 15 (4): 279-86. doi:10.1093/protein/15.4.279. PMID 11983928. Adhikari, AN; Peng, J; Wilde, M; Xu, J; Freed ... Fiser A, Gian Do RK, Sali A. (2000) Modeling of loops in protein structures" Protein Science 9: 1753-73 Ko J. et al. The FALC- ... Proteins: Structure, Function, and Bioinformatics 70, 1-10 (2008). Tang K., Zhang J., Liang J. (2014) Fast Protein Loop ... As mentioned above homology-based methods use a database to align the target protein gap with a known template protein. A ...
*  Hephaestin
Protein engineering. 15 (3): 205-214. doi:10.1093/protein/15.3.205. PMID 11932491. Huijun Chen; Trent Su; Zouhair K. Attieh; ... The protein was discovered and identified through the study of mice with sex-linked anemia, or sla mice, in which there is ... Hephaestin is a protein of 1135 aminoacids formed from a precursor of 1158 aminoacids and is 130.4 kDa. It is predicted to bind ... However, when the protein was ablated in murine models, both intestine-specific and whole-body hephaestin knockout (KO) strains ...
*  M. Vijayan
Protein Engineering. 5 (5): 399-404. doi:10.1093/protein/5.5.399. PMID 1518787. "Past Office Bearers - Year (2001-2003)". ... structural changes that accompany partial dehydration and protein action". Proteins. 71 (1): 241-249. doi:10.1002/prot.21695. ... Nagendra, H.G.; Sukumar, N.; Vijayan, M. (1 August 1998). "Role of water in plasticity, stability, and action of proteins: The ... Prabu, M.M.; Suguna, K.; Vijayan, M. (1 April 1999). "Variability in quaternary association of proteins with the same tertiary ...
*  Phage display
... is also a widely used method for in vitro protein evolution (also called protein engineering). As such, phage ... Phage display is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that ... a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on ... characterize small molecules-protein interactions and map protein-protein interactions. Users can use three dimensional ...
*  Streptomyces olivochromogenes
Protein engineering. 1 (6): 459-66. doi:10.1093/protein/1.6.459. PMID 3508293. Balachandran, Chandrasekar; Duraipandiyan, ...