*Guanidinium chloride

Lapange, Savo (1978). Physicochemical aspects of protein denaturation. New York: Wiley. ISBN 0-471-03409-6. ... At high concentrations of guanidinium chloride (e.g., 6 M), proteins lose their ordered structure, and they tend to become ... Guanidinium chloride is a strong chaotrope and one of the strongest denaturants used in physiochemical studies of protein ... but not prion protein aggregation in yeast. Mol Cell Biol 22 (15):5593-5605. Eaglestone SS, Ruddock LW, Cox BS, Tuite MF (2000 ...

*Bendazac

Principal action is inhibition of protein denaturation. Use of chloroacetamide in the alkylation step, followed by acid ...

*Terminal amine isotopic labeling of substrates

Inactivation of the proteases and protein denaturation and reduction. Labelling with stable isotopes. This allows peptides that ... This breaks each protein into fragments. The labeled N-termini of the original proteins remain blocked, while the new internal ... and as a way to define the termini of proteins that enables protein annotation. TAILS can also be used to link proteases with a ... The eluted unbound proteins are highly concentrated with the N-terminal peptides and neo-N-terminal peptides. This eluted ...

*Food extrusion

Denaturation of proteins. The material of which an extrusion die is made can affect the final product. Compared to stainless ... Texturized products include meat analogues, which are made using plant proteins ("textured vegetable protein") and a long die ... The process can induce both protein denaturation and starch gelatinization under some conditions. Many food extrusion processes ... It may also improve "protein quality and digestibility", and affects the product's shape, texture, colour, and flavour. It may ...

*Denaturation (biochemistry)

Protein denaturation is also a consequence of cell death. Denatured proteins can exhibit a wide range of characteristics, from ... In quaternary structure denaturation, protein sub-units are dissociated and/or the spatial arrangement of protein subunits is ... Protein folding is key to whether a globular protein or a membrane protein can do its job correctly. It must be folded into the ... Charles Tanford (1968), "Protein denaturation" (PDF), Advances in Protein Chemistry, 23: 121-282, doi:10.1016/S0065-3233(08) ...

*Bone cement

This temperature is higher than the critical level for protein denaturation in the body. The cause of the low polymerization ...

*Hydrophobic effect

Kauzmann W (1959). "Some factors in the interpretation of protein denaturation". Advances in Protein Chemistry. 14: 1-63. doi: ... The hydrophobic effect depends on the temperature, which leads to "cold denaturation" of proteins. Entropic force Hydrophobe ... In the case of protein folding, the hydrophobic effect is important to understanding the structure of proteins that have ... "Cold denaturation of a protein dimer monitored at atomic resolution". Nat. Chem. Biol. 9 (4): 264-70. doi:10.1038/nchembio.1181 ...

*Greenland shark

Bennion, Brian J.; Daggett, Valerie (2004). "Counteraction of urea-induced protein denaturation by trimethylamine N-oxide: a ... TMAO also counteracts the protein-destabilizing tendencies of urea and of deep-water pressure. Its presence in the tissues of ...

*Breeding for heat stress tolerance

High temperature directly affect injuries such as protein denaturation and aggregation, and increased fluidity of membrane ... Expression of heat shock proteins (HSPs), protein with 10 to 200 kDa, is supposed to be involved in signal transduction during ... The Stg1 and Stg2 regions also contain the genes for key photosynthetic enzymes, heat shock proteins, and an abscisic acid (ABA ... Other indirect or slower heat injuries involve inactivation of enzymes in chloroplast and mitochondria, protein degradation, ...

*Urinary cast

Cast formation is pronounced in environments favoring protein denaturation and precipitation (low flow, concentrated salts, low ... Given the ubiquitous presence of Tamm-Horsfall protein, other cast types are formed via the inclusion or adhesion of other ... They are pathognomonic for high urinary protein nephrotic syndrome. Formed by the adhesion of metabolic breakdown products or ... granular casts can result either from the breakdown of cellular casts or the inclusion of aggregates of plasma proteins (e.g., ...

*Silanization of silicon and mica

With metal surfaces, protein denaturation, unstable and reversible binding, nonspecific and random immobilization of protein ... First, the proteins adsorbed on the surface are more stable over a wide range of conditions. The proteins also adopt a more ... Nanoscale analysis of proteins using atomic force microscopy (AFM) requires surfaces with well-defined topologies and ... Biomolecules, particularly proteins, can be immobilized simply on an unmodified substrate surface through hydrophobic or ...

*Necrosis

Coagulation occurs as a result of protein denaturation, causing albumin to transform into a firm and opaque state. This pattern ... In adaptation to higher tendency of cell death, blind mole rats evolved a mutation in the tumor suppressor protein p53 (which ... and damage proteins and membranes. Antioxidant treatments can be applied to scavenge the ROS. Wounds caused by physical agents ...

*Fuzhou people

A famous protein scientist, Hsien Wu was the first to propose that protein denaturation was a purely conformational change. ... Hsien Wu, a Chinese protein scientist who was the first to propose that protein denaturation was a purely conformational change ... corresponded to protein unfolding and not to some chemical alteration of the protein. Zhang Yuzhe, a Chinese astronomer who is ...

*Cyclol

"Protein Denaturation and the Properties of Protein Groups". Advances in Protein Chemistry. Advances in Protein Chemistry. 2: ... "Some Factors in the Interpretation of Protein Denaturation". Advances in Protein Chemistry. Advances in Protein Chemistry. 14: ... Putnam, F (1953). "Protein Denaturation". The Proteins (H. Neurath and K. Bailey, eds.). 1B: 807-892. Astbury, WT; Woods HJ ( ... denaturation could involve a chemical change that converted folded proteins into polypeptides. The process of protein ...

*Harmonic scalpel

... which generates heat and causes protein denaturation. This technique causes minimal energy transfer to surrounding tissue, ...

*Glassblower's cataract

... denaturation. The rate of protein denaturation is determined by a rate constant that is temperature dependent as described by ... Large important biomolecules such as protein tend to lose their space structure when vibrating, ...

*Avidin

... dissociation of the avidin-biotin complex requires extreme conditions that cause protein denaturation. The non-reversible ... confirmed that the isolated egg protein was the cause of biotin deficiency or "egg white injury". At the time the protein had ... Advances in Protein Chemistry Volume. 29. pp. 85-133. doi:10.1016/S0065-3233(08)60411-8. ISBN 978-0-12-034229-7. Gyorgy, P.; ... Functional avidin is found only in raw egg, as the biotin avidity of the protein is destroyed by cooking. The natural function ...

*Alpha helix

This helix-coil transition was once thought to be analogous to protein denaturation. The statistical mechanics of this ... "The disposition of the LZCC protein residues in wenxiang diagram provides new insights into the protein-protein interaction ... In classifying proteins by their dominant fold, the Structural Classification of Proteins database maintains a large category ... Since 2001 Voss-Andreae creates "protein sculptures" based on protein structure with the α-helix being one of his preferred ...

*Luciferase

... is a heat-sensitive protein that is used in studies on protein denaturation, testing the protective capacities of ... Massoud TF, Paulmurugan R, De A, Ray P, Gambhir SS (Feb 2007). "Reporter gene imaging of protein-protein interactions in living ... is closely associated with a luciferin-binding protein as well as a green fluorescent protein (GFP). Calcium triggers release ... for many of the same applications as fluorescent proteins. However, unlike fluorescent proteins, luciferases do not require an ...

*Solvent impregnated resin

On the other hand, PEG appears to stabilize the protein molecules, which results in a comparatively low protein denaturation ... This is due to the sensitivity of bioproducts such as proteins towards organic extractants. One approach by C. van den Berg et ... A completely new approach of using SIRs for the separation or purification of biotechnological products such as proteins is ... During classical aqueous two-phase extraction, biotechnological components such as proteins are extracted from aqueous ...

*Protein folding

The external factors involved in protein denaturation or disruption of the native state include temperature, external fields ( ... Home Foldit computer game Potential energy of protein Protein dynamics Protein misfolding cyclic amplification Protein ... Such VCD studies of proteins are often combined with X-ray diffraction of protein crystals, FT-IR data for protein solutions in ... Cui D, Ou S, Patel S (December 2014). "Protein-spanning water networks and implications for prediction of protein-protein ...

*PSE meat

In addition, calmer animals will have a lower body temperature, which can help eliminate protein denaturation if no lairage ... which in turn causes protein denaturation and a loss of functionality, important factor to create meaty products, such as ... As the pH drops, proteins in the myofibers are denatured, leading to abnormal cell structure. The result is a pale tissue color ... The sarcomeres collapse excessively, and less water is held within the cell membrane and proteins. Subsequently, the myofibers ...

*Salting out

It is used as a method of purification for proteins, as well as preventing protein denaturation due to excessively diluted ... Unwanted proteins can be removed from a protein solution mixture by salting out as long as the solubility of the protein in ... The salt concentration needed for the protein to precipitate out of the solution differs from protein to protein. This process ... the protein can be dissolved in water. As a result of the increased demand for solvent molecules, the protein-protein ...

*Cleavable detergent

... that are used in biochemistry and especially in proteomics to enhance protein denaturation and solubility. The detergent is ... Applications for cleavable detergents include protease digestion of proteins such as in-gel digestion with trypsin after SDS ...

*Corrosive substance

... ester hydrolysis and protein denaturation. Proteins (chemically composed of amide bonds) are destroyed via amide hydrolysis ... For example, most drain cleaners contain either acids or alkalis due to their capabilities of dissolving greases and proteins ...

*Moist heat sterilization

Moist heat causes destruction of micro-organisms by denaturation of macromolecules, primarily proteins. Destruction of cells by ... The various procedures used to perform moist heat sterilization process cause destruction of micro-organisms by denaturation of ...