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*  DNA polymerase
Molecular cloning Polymerase chain reaction Protein domain dynamics Reverse transcription RNA polymerase Taq DNA polymerase ... Family X polymerases contain the well-known eukaryotic polymerase pol β (beta), as well as other eukaryotic polymerases such as ... Prokaryotic Polymerase Prokaryotes only have one RNA polymerase and it exists in two forms: core polymerase and holoenzyme. ... No known DNA polymerase is able to begin a new chain (de novo); it can only add a nucleotide onto a pre-existing 3'-OH group, ...
*  Multiplex polymerase chain reaction
... (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different ... as if performing many separate PCR reactions all together in one reaction). This process amplifies DNA in samples using ... Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon ... multiple primers and a temperature-mediated DNA polymerase in a thermal cycler. The primer design for all primers pairs has to ...
*  Thermal cycler
Weier, HU; Gray, JW (Jul-Aug 1988). "A programmable system to perform the polymerase chain reaction". DNA (Mary Ann Liebert, ... is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). Thermal ... The device has a thermal block with holes where tubes holding the reaction mixtures can be inserted. The cycler then raises and ... This led to a cumbersome machine based on an automated pipettor, with open reaction tubes. Later, the PCR process was adapted ...
*  DNA polymerase I
In fact, the Klenow fragment was used during the first protocols of polymerase chain reaction (PCR) amplification until Thermus ... Studies of polymerase I have confirmed that different dNTPs can bind to the same active site on polymerase I. Polymerase I is ... DNA polymerase II DNA polymerase III Template:Cite journal,. So also known as kornberg polymerase. Voet D, Voet JG, Pratt CW ( ... DNA polymerases also cannot initiate DNA chains so they must be initiated by short RNA or DNA segments known as primers. In ...
*  T7 DNA polymerase
... the reaction is metal-dependent and cations such as Mg2+ are often present in the enzyme active site. For T7 DNA polymerase, ... the chain-terminating nucleotides. Most of known DNA polymerases strongly discriminate against ddNTP; and thus, a high ratio of ... It is a member of the Family A DNA polymerases, which include E. coli DNA polymerase I and Taq DNA polymerase. This polymerase ... T7 DNA polymerase is an enzyme used during the DNA replication of the T7 bacteriophage. During this process, the DNA polymerase ...
*  Nested polymerase chain reaction
... involves two sets of primers, used in two successive runs of polymerase chain reaction, the ... Nested polymerase chain reaction (Nested PCR) is a modification of polymerase chain reaction intended to reduce non-specific ... Books at your local library about nested polymerase chain reactions Scholarly articles on nested polymerase chain reactions ... Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature-mediated DNA polymerase. The ...
*  Inverse polymerase chain reaction
... (Inverse PCR) is a variant of the polymerase chain reaction that is used to amplify DNA with ... Then, like other polymerase chain reaction processes, the DNA is amplified by the temperature-sensitive DNA polymerase: A ... Ochman, H.; Gerber, A. S.; Hartl, D. L. (1988-11-01). "Genetic applications of an inverse polymerase chain reaction". Genetics ...
*  Molecular cloning
... is similar to polymerase chain reaction (PCR) in that it permits the replication of DNA sequence. The ... Polymerase chain reaction (PCR) methods are often used for amplification of specific DNA or RNA (RT-PCR) sequences prior to ... polymerase chain reaction, restriction fragment analysis and/or DNA sequencing. Molecular cloning provides scientists with an ... This joining reaction is often termed ligation. The resulting DNA mixture containing randomly joined ends is then ready for ...
*  Touchdown polymerase chain reaction
The touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction ... The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point ... Nonspecific primer binding obscures polymerase chain reaction results, as the nonspecific sequences to which primers anneal in ... The earliest steps of a touchdown polymerase chain reaction cycle have high annealing temperatures. The annealing temperature ...
*  Polymerase chain reaction inhibitors
PCR inhibitors are any factor which prevent the amplification of nucleic acids through the polymerase chain reaction (PCR). PCR ... or otherwise interfering with their interaction with the DNA polymerase, PCR is inhibited. In a multiplex PCR reaction, it is ... In order to try to assess the extent of inhibition that occurs in a reaction, a control can be performed by adding a known ... Of course, if any part of the inhibition occurring in the sample-derived reaction mixture is sequence-specific, then this ...
*  Digital polymerase chain reaction
The polymerase chain reaction method is used to quantify nucleic acids by amplifying a nucleic acid molecule with the enzyme ... Digital polymerase chain reaction (digital PCR, DigitalPCR, dPCR, or dePCR) is a biotechnological refinement of conventional ... polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly", issued 2000-11-07, ... "Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification". Analytical Chemistry. 84 ...
*  Polymerase chain reaction optimization
Touchdown polymerase chain reaction or touchdown style polymerase chain reaction is a method of polymerase chain reaction by ... Eckert KA, Kunkel TA (August 1991). "DNA polymerase fidelity and the polymerase chain reaction". PCR Methods Appl. 1 (1): 17-24 ... The polymerase chain reaction (PCR) is a commonly used molecular biology tool for amplifying DNA, and various techniques for ... Markoulatos P, Siafakas N, Moncany M (2002). "Multiplex polymerase chain reaction: a practical approach". J. Clin. Lab. Anal. ...
*  History of polymerase chain reaction
Repeated applications of polymerase could lead to a chain reaction of replication for a specific segment of the genome - PCR. ... "Fine-structure genetic mapping of human chromosomes using the polymerase chain reaction on single sperm." Am J Hum Genet vol. ... 263-73 (1986). Mullis KB and Faloona FA "Specific Synthesis of DNA in vitro via a Polymerase-Catalyzed Chain Reaction." Methods ... 341-61 (1971). Mullis KB, Ferré F, Gibbs RA "The Polymerase Chain Reaction" Birkhäuser Press (1994) ISBN 0-8176-3750-8 Chien A ...
*  Overlap extension polymerase chain reaction
The overlap extension polymerase chain reaction (or OE-PCR) is a variant of PCR. It is also referred to as Splicing by overlap ... This page assumes familiarity with the terms and components used in the polymerase chain reaction (PCR) process. ... The replication reaction continues to produce a fully dimerised DNA fragment. After further PCR cycles, to amplify the DNA, the ... As in most PCR reactions, two primers-one for each end-are used per sequence. To splice two DNA molecules, special primers are ...
*  Reverse transcription polymerase chain reaction
... (RT-PCR), a variant of polymerase chain reaction (PCR), is a technique commonly ... RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike, but they are ... The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in ... Bustin SA (October 2000). "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction ...
*  Template switching polymerase chain reaction
Template-switching polymerase chain reaction (TS-PCR) is a method of reverse transcription and polymerase chain reaction (PCR) ...
*  Real-time polymerase chain reaction
A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a ... The polymerase chain reaction (PCR) is a common method for amplifying DNA; for RNA-based PCR the RNA sample is first reverse- ... Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR), which is based on the analysis of RNA rather than DNA, ... "Robust Quantification of Polymerase Chain Reactions Using Global Fitting". PLOS ONE. 7 (5): e37640. doi:10.1371/journal.pone. ...
*  Biomedicine
Polymerase chain reaction is done by placing a mixture of the desired DNA, DNA polymerase, primers, and nucleotide bases into a ... ISBN 978-1-59734-971-0. "Polymerase chain reaction". "Gel electrophoresis". "Southern blot". Schopf, J. William. Life's Origin ... Molecular biology consists of different techniques including Polymerase chain reaction, Gel electrophoresis, and macromolecule ... Proteins are chains of amino acids that function to contract skeletal muscle, function catalysts, transport molecules, and ...
*  Glossary of biology
placebo . plant nutrition . plasmolysis . pollination . polygene . polymer . polymerase chain reaction . polyploid . population ... an endergonic reaction (also called a nonspontaneous reaction or an unfavorable reaction) is a chemical reaction in which the ... chemical kinetics The study and discussion of chemical reactions with respect to reaction rates, effect of various variables, ... flagellum . flavin adenine dinucleotide . food chain The chain of eating and getting nutrition which starts from a small ...
*  Brucellosis
by Polymerase Chain Reaction". Croatian Medical Journal. 51 (4): 306-313. doi:10.3325/cmj.2010.51.306. Vrioni, Georgia; Pappas ... The inability to diagnose B. canis by SAT due to lack of cross-reaction is another drawback. False-negative SAT may be caused ... Demonstration of antibodies against the agent either with the classic Huddleson, Wright, and/or Bengal Rose reactions, either ... and demonstrate positive Bengal Rose and Huddleston reactions. Gastrointestinal symptoms occur in 70% of cases and include ...
*  Cutaneous leishmaniasis
The gold standard for diagnosis is PCR (polymerase chain reaction) helps DNA polymerase to create new strands of DNA equivalent ... "Polymerase Chain Reaction (PCR)". www.ncbi.nlm.nih.gov. Retrieved 2016-06-10. Alrajhi AA, Ibrahim EA, De Vol EB, et al. (2002 ...
*  Ligand binding assay
Real-time polymerase chain reaction. The liquid phase ligand binding assay of Immunoprecipitation (IP) is a method that is used ... Mullis, K.B; Faloona, F.L. (1987). "[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction". Methods ... and the reliability and validity of linked reactions. Although binding assays are simple, they fail to provide information on ...
*  Vestibular papillomatosis
A polymerase chain reaction study". Arch Dermatol Res. 285 (5): 250-4. doi:10.1007/bf00371592. PMID 8397492. CS1 maint: ...
*  Santiago Schnell
doi:10.1006/jtbi.1996.0283 S. Schnell and C. Mendoza (1997). Theoretical description for polymerase chain reaction. Journal of ... Enzymological considerations for a theoretical description of the Quantitative Competitive Polymerase Chain Reaction (QC-PCR). ... His methods for modeling enzyme catalyzed reactions and reactions inside cells are one of the fast moving frontiers in ... His work has focused to resolve the ambiguities in the quantitative analysis and modeling of reactions inside cells. He has ...
*  Kary Mullis
The unusual origin of the polymerase chain reaction. Scientific American, April 56-65. The Polymerase Chain Reaction, 1994, co- ... In recognition of his invention of the polymerase chain reaction (PCR) technique, he shared the 1993 Nobel Prize in Chemistry ... He came up with the polymerase chain reaction while driving along a highway. In his 1998 autobiography, Mullis expressed ... Kary Mullis (December 8, 1993). "Nobel Lecture - The Polymerase Chain Reaction". nobelprize.org. Kary Mullis: Play! Experiment ...
*  Camelpox
Additionally, researchers in India are working on a diagnostic polymerase chain reaction to quickly and effectively identify ... Balamurugan, Vinayagamurthy (March 2009). "A Polymerase Chain Reaction Strategy for the Diagnosis of Camelpox". Journal of ... The virus carries DNA polymerase which is used to transcribe its genes. Eventually, the viral core dissolves, and the genetic ... a broad spectrum anti-viral that acts by inhibiting the viral DNA polymerase. Cidofovir has proven to be 100% effective at ...
*  Molecular biology
"Polymerase Chain Reaction (PCR)". www.ncbi.nlm.nih.gov. Retrieved 31 December 2016. "Polymerase Chain Reaction (PCR) Fact Sheet ... In this technique, DNA coding for a protein of interest is cloned using polymerase chain reaction (PCR), and/or restriction ... Polymerase chain reaction (PCR) is an extremely versatile technique for copying DNA. In brief, PCR allows a specific DNA ... The reaction is extremely powerful and under perfect conditions could amplify one DNA molecule to become 1.07 billion molecules ...