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*  Relaxosome
The proteins are encoded by the tra operon on a fertility plasmid in the region near the origin of transfer, oriT. The most ... This nicking results in a DNA-Protein complex with the relaxosome bound to a single strand of the plasmid DNA and an exposed 3 ... Relaxase also unwinds the plasmid being conjugated with its helicase properties. The relaxosome interacts with integration host ... The Relaxosome' is the complex of proteins that facilitates plasmid transfer during bacterial conjugation. ...
*  Extrachromosomal DNA
Fertility plasmids, or f plasmids, allow for conjugation to occur whereas resistance plasmids, or r plasmids, contain genes ... Circular bacterial plasmids are classified according to the special functions that the genes encoded on the plasmid provide. ... Circular bacterial plasmids are also the basis for the production of DNA vaccines. Plasmid DNA vaccines are genetically ... The linear plasmids with a covalently attached protein may assist with bacterial conjugation and integration of the plasmids ...
*  Plasmid-mediated resistance
... is the transfer of antibiotic resistance genes which are carried on plasmids. The plasmids can be ... Other smaller plasmids (usually < 10 kb in size) can be mobilized by a conjugative plasmid (usually > 30 kb) in order to be ... are common for resistance plasmids in Enterobacteriaceae. Often multiple beta-lactamase genes are found on the same plasmid ... It is very common for the resistance genes or entire resistance cassettes to be re-arranged on the same plasmid or be moved to ...
*  Plasmid partition system
Each plasmid has its independent replication system which controls the number of copies of the plasmid in a cell. The higher ... pSK1 is a plasmid from Staphylococcus aureus. This plasmid has a partition system determined by a single gene, par, previously ... For instance, if there are 2 copies of a plasmid in a cell, there is a 50% chance of having one plasmid-less daughter cell. ... A plasmid partition system is a mechanism that assures the stable transmission of plasmids during bacterial cell division. ...
*  Multiple cloning site
One bacterial plasmid used in genetic engineering as a plasmid cloning vector is pUC18. Its polylinker region is composed of ... Restriction sites within an MCS are typically unique, occurring only once within a given plasmid. MCSs are commonly used during ...
*  Subcloning
... creating a new plasmid which contains the background elements of the original plasmid with a different insert. The plasmid is ... The plasmid is often transformed into a bacterium like E. coli. Ideally when the bacterium divides the plasmid should also be ... a gene from mammalian gene library will be subcloned into a bacterial plasmid (destination platform). The bacterial plasmid is ... If the bacteria that were supposed to pick up the desired plasmid had picked up the desired gene then they would also contain ...
*  Molecular cloning
Cells harboring the plasmid will survive when exposed to the antibiotic, while those that have failed to take up plasmid ... "plasmid / plasmids , Learn Science at Scitable". www.nature.com. Retrieved 2017-12-06. Shizuya H, Birren B, Kim UJ, Mancino V, ... and a plasmid cloning vector. E. coli and plasmid vectors are in common use because they are technically sophisticated, ... That is, these plasmids could serve as cloning vectors to carry genes. Virtually any DNA sequence can be cloned and amplified, ...
*  Transformation efficiency
Forms of DNA - Supercoiled plasmid have a slightly better transformation efficiency than relaxed plasmids - relaxed plasmids ... 108 cfu/μg for a small plasmid like pUC19 is roughly equivalent to 1 in 2000 molecules of the plasmid used being transformed. ... coli found that transformation efficiency declines linearly with increasing plasmid size, i.e. larger plasmids transform less ... The standard plasmids used for determination of transformation efficiency in Escherichia coli are pBR322 or other similarly- ...
*  Hybrid plasmid
... is a plasmid that contains an inserted piece of foreign DNA. "Everything Bio : Hybrid plasmid". Everythingbio. ...
*  Plasmid RNAIII
... is a non-coding RNA found in bacterial plasmids including pIP501. RNAIII acts by transcriptional attenuation of ... importance of a U-turn loop structure in the target RNA of plasmid pIP501 for efficient inhibition by the antisense RNA". J Mol ...
*  Ti plasmid
The plasmid has 196 genes that code for 195 proteins. There is one structural RNA. The plasmid is 206,479 nucleotides long, the ... A Ti or tumour inducing plasmid is a plasmid that often, but not always, is a part of the genetic equipment that Agrobacterium ... Agrobacterium tumefaciens plasmid pTi-SAKURA, complete sequence Ti Plasmid Genetic Map. ... The Ti plasmid is lost when Agrobacterium is grown above 28 °C. Such cured bacteria do not induce crown galls, i.e. they become ...
*  RK2 plasmid
The IncP-1 plasmid group (IncP plasmids in Escherichia coli) of which RK2 is a part has been described as "highly potent, self- ... The RK2 Plasmid is a broad-host-range plasmid belonging to the incP incompatibility group It is notable for its ability to ... Minimal plasmids such as PFF1 are useful for studying the basic mechanisms of plasmid replication and copy number regulation, ... Plasmid, 2010 Nov;64(3):119-34 U Kües and U Stahl: "Replication of plasmids in gram-negative bacteria", Microbiol Rev. 1989 ...
*  R1 plasmid
The R1 Plasmid is a plasmid that was first isolated from Salmonella paratyphi bacteria in 1963. The R1 plasmid imparts multi- ... It's known as a "low copy" plasmid, meaning that it exists in relatively few copies in any given bacteria. Because of its low ... Some genes on the R1 plasmid are: ParM is a prokaryotic actin homologue which provides the force to drive copies of the R1 ... The Hok/sok system a postsegregational killing system of the plasmid. CopA-like RNA, an antisense RNA involved in replication ...
*  Plasmid preparation
A typical plasmid DNA yield of a miniprep is 50 to 100 µg depending on the cell strain. Miniprep of large number of plasmids ... Minipreparation of plasmid DNA is a rapid, small-scale isolation of plasmid DNA from bacteria. It is based on the alkaline ... A plasmid preparation is a method of DNA extraction and purification for plasmid DNA. Many methods have been developed to ... The plasmid DNA yield will vary depending on the plasmid copy number, type and size, the bacterial strain, the growth ...
*  Plasmid copy number
Plasmids may be either high copy number plasmids or low copy number plasmids; the regulation mechanisms between these two type ... When the plasmid concentration is high, RepA plasmids bound to iterons form dimers in between two plasmids, "handcuffing" them ... Low copy number plasmids, also called stringent plasmids, require tighter control of replication. In ColE1 derived plasmids, ... of the plasmid). This is particularly likely with low copy number plasmids. Plasmids can also be incompatible due to shared ...
*  Bacillus-plasmid RNA motif
The Bacillus-plasmid RNA motif is a predicted conserved RNA structure usually located in plasmids. It is known in species under ... The fact that the RNA structure is typically found in plasmids suggests that it might be involved in the regulation plasmid ... However, the Bacillus-plasmid RNA motif does not appear to be homologous to any such known RNA. Weinberg Z, Wang JX, Bogue J, ... Kim K, Meyer RJ (October 1986). "Copy-number of broad host-range plasmid R1162 is regulated by a small RNA". Nucleic Acids Res ...
*  Lactis-plasmid RNA motif
However, many of the plasmids that carry lactis-plasmid RNAs also carry ctRNA-pND324 RNAs, which are involved in plasmid copy ... Therefore lactis-plasmid RNAs might have a different function. R1162-like plasmid antisense RNA Weinberg Z, Wang JX, Bogue J, ... They typically lie near to repB genes, and are almost found in plasmids. This data suggested that lactis-plasmid RNAs ... The lactis-plasmid RNA motif is a conserved RNA structure identified by bioinformatics. The RNAs are restricted to lactic acid ...
*  R1162-like plasmid antisense RNA
... is a 75-base RNA molecule which negatively regulates the RepI region of the plasmid. The ... "Copy number of the broad host-range plasmid R1162 is determined by the amounts of essential plasmid-encoded proteins". Journal ... Kim, K; Meyer RJ (1986). "Copy-number of broad host-range plasmid R1162 is regulated by a small RNA". Nucleic Acids Res. 14 (20 ... Experimental evidence has shown that in Escherichia coli, when levels of this RNA are decreased, the plasmid copy number of ...
*  Human HGF plasmid DNA therapy
... of cardiomyocytes is being examined as a potential treatment for coronary artery disease (a major ... Transfection with HGF plasmids in damaged cardiac tissue also promotes angiogenesis (increased capillary density compared to ... Animal research has demonstrated that administration of HGF cDNA plasmids into ischemic cardiac tissue can increase cardiac ... "Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ...
*  Spinach aptamer
SPINACH PLASMIDS. ...
*  Archaea
Smaller independent pieces of DNA, called plasmids, are also found in archaea. Plasmids may be transferred between cells by ... Sota M; Top EM (2008). "Horizontal Gene Transfer Mediated by Plasmids". Plasmids: Current Research and Future Trends. Caister ... ISBN 978-1-904455-27-1. Lipps G (2008). "Archaeal Plasmids". Plasmids: Current Research and Future Trends. Caister Academic ... Schleper C; Holz I; Janekovic D; Murphy J; Zillig W (1 August 1995). "A multicopy plasmid of the extremely thermophilic ...
*  Biotechnology in pharmaceutical manufacturing
Plasmids containing the Factor IX gene, along with plasmids with a gene that codes for resistance to methotrexate, were ... The recombinant plasmids were inserted into Escherichia coli bacteria, which were "induced to produce 100,000 molecules of ... The gene that was inserted into the plasmid was created by reverse transcription of the mRNA found in pituitary glands to ... The artificial genes were "then inserted... into plasmids... among a group of genes that" are activated by lactose. Thus, the ...
*  Lipofectamine
It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures ... DNA plasmids. Lipofectamine is a cationic liposome formulation, which complexes with negatively charged nucleic acid molecules ...
*  Streptomyces fimbriatus
Plasmids in Bacteria. Boston, MA: Springer US. ISBN 1-4613-2447-5. CS1 maint: Extra text: authors list (link) Roberts, edited ...
*  Streptomyces ghanaensis
Plasmid. 61 (3): 171-175. doi:10.1016/j.plasmid.2008.12.002. Labes, G.; Bibb, M.; Wohlleben, W. (1 May 1997). "Isolation and ... Plasmid. 61 (3): 171-175. doi:10.1016/j.plasmid.2008.12.002. ATCC Type strain of Streptomyces ghanaensis at BacDive - the ... Muth, Gunter; Wohlleben, Wolfgang; Pohler, Alfred (March 1988). "The minimal replicon of the Streptomyces ghanaensis plasmid ... Plasmids in Bacteria. Boston, MA: Springer US. ISBN 1-4613-2447-5. CS1 maint: Extra text: authors list (link) ed.-in-chief, ...