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*  Peptide library
A peptide library is a tool for protein-related study. A peptide library contains a great number of peptides that have a ... peptide library is synthesized on solid phase, mostly on resin, which can be made as flat surface or beads. The peptide library ... having the library of different peptides at residue 2 and 3 would let you see if some change in chemical properties in the N- ... As a general formula, if you made a completely random peptide library with n amino acids for each link in the chain with a ...
*  Peptide spectral library
... which will continuously expand the coverage of peptide spectral library. For a peptide spectra library, to reach a maximal ... A peptide spectral library is a curated, annotated and non-redundant collection/database of LC-MS/MS peptide spectra. One ... the unknown spectra can be assigned the identity of the known peptide in the library. Spectral libraries have been used in the ... The research community focused peptide spectral library supports targeted research in a comprehensive fashion for a particular ...
*  Sigma-Aldrich
... isotopically-labelled peptides and peptide libraries. Sigma Advanced Genetic Engineering (SAGE) Labs is a division within Sigma ... ISOTEC provides isotopically labeled products for protein structure determination, peptide synthesis, proteomics, metabolic ... peptides, amino acids and their derivatives. Sigma RBI produces specialized products for use in the field of cell signaling and ...
*  Amino acid
... has made peptide synthesis particularly important in creating libraries of peptides for use in drug discovery through high- ... "Past and future perspectives of synthetic peptide libraries". Current Protein & Peptide Science. 9 (5): 447-67. doi:10.2174/ ... However, not all peptide bonds are formed in this way. In a few cases, peptides are synthesized by specific enzymes. For ... In chemistry, peptides are synthesized by a variety of reactions. One of the most-used in solid-phase peptide synthesis uses ...
*  In vitro compartmentalization
135(3): p. 285-8. Yonezawa, M., et al., DNA display for in vitro selection of diverse peptide libraries. Nucleic Acids Res, ... When a protein/peptide is expressed in fusion with Agrobacterium protein VirD2, it will bind to its DNA coding sequence that ... The phenotype to be selected can range from RNA to peptide to protein. By selecting for binding, the most commonly evolved ... All the newly formed proteins/peptides will be in fusion with streptavidin molecules and bind to their biotinylated coding ...
*  Pathophysiology of spider bites
Escoubas P (November 2006). "Molecular diversification in spider venoms: a web of combinatorial peptide libraries". Molecular ...
*  PFKFB2
2001). "Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6- ...
*  MRNA display
A DNA library for any protein or small peptide of interest can be synthesized by solid-phase synthesis followed by PCR ... They allow a library size as large as 10^15 different members. The large library size increases the probability to select very ... For example, the library size for phage and bacterial display is limited to 1-10 × 10^9 different members. The library size for ... The T7 promoter region allows large-scale in vitro T7 transcription to transcribe the DNA library into an mRNA library, which ...
*  Lewis C. Cantley
In later research, the oriented peptide library approach has also been used to characterize protease cleavage specificity. ... peptide library approach was extended to identify the substrate specificity of protein kinases toward synthetic peptides. This ... "Use of an oriented peptide library to determine the optimal substrates of protein kinases". Curr. Biol. 4 (11): 973-82. doi: ... "Determination of protease cleavage site motifs using mixture-based oriented peptide libraries". Nat. Biotechnol. 19 (7): 661-7 ...
*  Kim Janda
"A cell-penetrating peptide from a novel pVII-pIX phage-displayed random peptide library". Bioorganic & Medicinal Chemistry. 10 ... By panning this library against a B lymphoctye cell line, a unique cell-binding and internalizing peptide was discovered. ... These findings were crucial to his group's publication on the glycation of the amyloid β-peptide by nornicotine, proposing the ... Janda has also worked creating peptide and antibody molecules for the treatment of cancer. By employing a novel approach, he ...
*  Anogen
"PSMA mimotope isolated from phage displayed peptide library can induce PSMA specific immune response". Cell research. 9 (4): ... synthetic bioactive peptides, and Enzyme linked immunosorbent assay (ELISA) kits for research as well as diagnostic and ...
*  DNA-encoded chemical library
According to this split-and-pool protocol a peptide combinatorial library DNA-encoded of 106 members was generated. In 2001 ... A sub-library can be paired with a complementary oligonucleotide and used as a DNA encoded library displaying a single ... "Generation and screening of an oligonucleotide-encoded synthetic peptide library". Proc. Natl. Acad. Sci. U.S.A. 90 (22): 10700 ... to a DNA oligonucleotide tag before library assembly, therefore more upfront work is required before library assembly. ...
*  Combinatorial chemistry
This approach was also used in screening peptide libraries. The tethered peptide library was tested with a dissolved target ... If we deal with a non-peptide organic libraries library it is not as simple to determine of the identity of the content of a ... "amino acid tester libraries" that contain those peptides that are missing from the omission libraries can also be used in the ... If a combinatorial peptide library is synthesized using 20 amino acids (or other kinds of building blocks) the bead form solid ...
*  Phage display
"T7 displayed peptides as targets for selecting peptide specific scFvs from M13 scFv display libraries". J. Immunol. Methods. ... Peptides are usually fused to the N-terminus of pVIII. Usually peptides that can be fused to pVIII are 6-8 amino acids long. ... "A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17 ... "Comparison of bacterial and phage display peptide libraries in search of target-binding motif". Appl. Biochem. Biotechnol. 127 ...
*  Ribosome display
"An in vitro polysome display system for identifying ligands from very large peptide libraries". Affymax Research Institute. 91 ... However, the DNA library coding for a particular library of binding proteins is genetically fused to a spacer sequence lacking ... peptides (Mattheakis, Bhatt and Dow) As it is performed entirely in vitro, there are two main advantages over other selection ... First, the diversity of the library is not limited by the transformation efficiency of bacterial cells, but only by the number ...
*  OmpT
"Substrate specificity of the integral membrane protease OmpT determined by spatially addressed peptide libraries". Biochemistry ... Once docked in this position, water is positioned to attack the peptide in the active site. The cleavage of peptide bonds by ... The peptide bond cleavage occurs via the nucleophilic attack of water at the carbonyl between two adjacent amino acid residues ... Because of the specificity of the active site, OmpT does not act on peptides with a negatively charged residue adjacent to the ...
*  Bacterial display
2005). "Comparison of Bacterial and Phage Display Peptide Libraries in Search of Target-Binding Motif". Applied Biochemistry ... fused peptides with linkers with the ompA gene, causing the peptides to be expressed in the OmpA proteins. They showed that the ... Peptides are very useful as therapeutic and diagnostic substances. Their use is getting more popular, and display systems offer ... Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection ...
*  Chromatophore
... lawn format assay to rapidly screen a 442,368 bead-based peptide library". J Pharmacol Toxicol Methods. 42 (4): 189-97. doi: ... Porras, MG; De Loof, A; Breuer, M; Aréchiga, H (2003). "Procambarus clarkii". Peptides. 24 (10): 1581-9. doi:10.1016/j.peptides ...
*  Degradomics
Plant, 145, 5-17 (2012). Schilling O., Overall C.M. Proteome-derived, database-searchable peptide libraries for identifying ... Beginning with a peptide library generated from endopeptidase digestion of a proteome, this technique allows for screening and ... Protease Specificity Profiling by Tandem Mass Spectrometry Using Proteome-derived Peptide Libraries. Gel-Free Proteomics: ... 21, 566-569 (2003). Chen S.-H., Chen C.-R., Chen S.-H., Li D.-T., Hsu J.-L. Improved N(α)-acetylated peptide enrichment ...
*  Mimotope
These peptides were extracted from biopanning results of phage-displayed random peptide libraries reported in 571 papers. The ... However, this concept has been extended to refer peptide mimic of all types of binding sites. As the mimic of binding site, ... A mimotope is a macromolecule, often a peptide, which mimics the structure of an epitope. Because of this property it causes an ... In version 2.0, it has 15633 peptides collected from 849 papers and grouped into 1818 sets. Besides the core data on panning ...
*  Calpain
"Determination of peptide substrate specificity for mu-calpain by a peptide library-based approach: the importance of primed ... Amongst peptide and small-molecule substrates, the most consistently reported specificity is for small, hydrophobic amino acids ... ISBN 1-56032-713-8. Calpain at the US National Library of Medicine Medical Subject Headings (MeSH) CaMPDB, Calpain for ...
*  Biopanning
All peptide sequences obtained from biopanning using combinatorial peptide libraries have been stored in a special freely ... Libraries of peptides and proteins displayed on filamentous phage. Methods in Enzymology. 1993. 217:228-257. ... Biopanning involves 4 major steps for peptide selection. The first step is to have phage display libraries prepared. This ... Mandecki W, Chen YC, and Grihalde N. A Mathematical Model for Biopanning (Affinity Selection) Using Peptide Libraries on ...
*  Creative Biolabs
Pre-made phage display library is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA ... Fukunaga, K; Taki, M. "Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially ... such as Anti-ADP3 chicken IgY Antibody libraries such as HuSdLTM phage display human single domain antibody library "Creative ... including various phage display libraries such as pre-made libraries, phage display services, antibody sequencing, and antibody ...
*  Peptide deformylase
"Determination of substrate specificity for peptide deformylase through the screening of a combinatorial peptide library". ... In enzymology, a peptide deformylase (EC 3.5.1.88) is an enzyme that catalyzes the chemical reaction formyl-L-methionyl peptide ... Pei D (2001). "Peptide deformylase: a target for novel antibiotics?". Emerging Therapeutic Targets. 5 (1): 23-40. doi:10.1517/ ... Chan MK, Gong W, Rajagopalan PT, Hao B, Tsai CM, Pei D (1997). "Crystal structure of the Escherichia coli peptide deformylase ...
*  Cooking banana
... fruit with combinatorial peptide ligand libraries". Electrophoresis. 34 (2): 207-14. doi:10.1002/elps.201200389. PMID 23161558 ...
*  TENM3
The peptide cleaved from the C terminal of Ten-m3, TCAP-3, stimulates the production of cAMP and the proliferation of neurons. ... The embryonic Drosophila cDNA library was screened using polymerase chain reaction (PCR) and a primer derived from the EGF-like ... The TCAP is the resulting peptide from cleaving a putative furin cleavage site found immediately on the N-terminal of TCAP. The ... Lovejoy DA, Pavlović T (November 2015). "Role of the teneurins, teneurin C-terminal associated peptides (TCAP) in reproduction ...