DNA Guanosine triphosphate Nucleoside Nucleotide Oligonucleotide RNA Crane, Laura J; Miller, David Lee (1974). "Guanosine ...
"Probing the Structure of Supported Membranes and Tethered Oligonucleotides by Fluorescence Interference Contrast Microscopy". ...
80-100 bp oligonucleotides can be generated as single molecules, or pairs of oligonucleotides that are completely overlapping ... Fully complementary oligonucleotides pairs lead to 5-10 fold increase in efficiency compared to single oligonucleotides and are ... To increase the efficiency, partly overlapping oligonucleotides can be extended in vitro. Oligonucleotides containing the ... To increase oligonucleotide targeting to the CORE cassette, CORE cassettes containing the GAL1-I-SceI feature can be used. This ...
7-nt primer library, 5′-NNNNNNrX-3′, are used in the ligation of a short degenerate oligonucleotide fragmentin, in which N ... When the DNA hairpin is unzipped into single-strand, oligonucleotides complementary to the hairpin sequence are allowed to ... During the time course of the rezipping process, the bound oligonucleotides cause transient blockages. The time course ... as well as the presence of mismatches between the oligonucleotide and the hairpin. Hybridization is one way to determine the ...
O'Keefe SJ, Wolfes H, Kiessling AA, Cooper GM (September 1989). "Microinjection of antisense c-mos oligonucleotides prevents ... O'Keefe SJ, Wolfes H, Kiessling AA, Cooper GM (September 1989). "Microinjection of antisense c-mos oligonucleotides prevents ...
Follow on work by Rosenthal achieved editing of mutated mRNA sequence in mammalian cell culture by directing an oligonucleotide ... This initial work used synthetic RNA antisense oligonucleotides complementary to a pre-mature stop codon mutation in a ... creating oligonucleotide primers). Whereas methods such as RNA interference (RNAi) do not fully suppress gene function, CRISPR ... "Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems". PLOS ONE. 10 (6): ...
This involved two closely related methods that generated short oligonucleotides with defined 3' termini. These could be ... "Use of DNA Polymerase I Primed by a Synthetic Oligonucleotide to Determine a Nucleotide Sequence in Phage f1 DNA", Proceedings ...
"Multicellular Tumor Spheroids as a Model for Assessing Delivery of Oligonucleotides in Three Dimensions". Molecular Therapy: ...
The genome as a whole is strikingly organized with respect to the local direction of replication; guanines, oligonucleotides ...
OMe RNA oligonucleotides". Cell. 59 (3): 531-9. doi:10.1016/0092-8674(89)90036-6. PMID 2478298. S2CID 45969803. Raghunathan PL ...
This involved two closely related methods that generated short oligonucleotides with defined 3' termini. These could be ...
The chain length of produced oligonucleotides is dependent on the pore size of CPG. In addition, porous glasses are used for ... with pore sizes between 50 and 300 nm is also excellently suited for the synthesis of oligonucleotides. In this application, a ...
Acryl oligonucleotides have been tested, but the acryl group is not stable to storage. Acrydite-modified oligonucleotides can ... Acrydite-modified oligonucleotides can be obtained from vendors such as Integrated DNA Technologies (IDT). The first use of ... Acrydite-modified oligonucleotides have been used to study protein DNA interactions Acrydite-modified aptamers (and also ... Acrydite is a phosphoramidite that allows the synthesis of oligonucleotides with a methacryl group at the 5' end (less commonly ...
The expression of D5 receptor in vitro can also be silenced using antisense oligonucleotides. Dopamine receptor GRCh38: Ensembl ...
The compound is also being explored for development of antisense oligonucleotides that can be cross-linked specifically to a ... "Synthesis and properties of photo-reactive antisense oligonucleotides containing 2'-O-psoralen-conjugated adenosine". Nucleic ...
"A Factorial Analysis of Silanization Conditions for the Immobilization of Oligonucleotides on Glass Surfaces". Analytical ...
Each nanowell contains oligonucleotides that provide an anchoring point for the adapters to attach. Once the fragments have ... The flow cell has oligonucleotides (short nucleotide sequences) coating the bottom of the cell, and they serve as the solid ... The now double stranded DNA is denatured so that each strand can separately attach to an oligonucleotide sequence anchored to ... Adapters contain three different segments: the sequence complementary to solid support (oligonucleotides on flow cell), the ...
This mini analyzer can measure 66k Da proteins, mixtures of oligonucleotides, and biological spores. Verbeck at University of ...
Morpholino-type antisense oligonucleotides, with the same cellular target as nusinersen, remain a subject of research in ... "Systemic peptide-mediated oligonucleotide therapy improves long-term survival in spinal muscular atrophy". Proceedings of the ... "Antisense oligonucleotides delivered to the mouse CNS ameliorate symptoms of severe spinal muscular atrophy". Science ...
Production of short single-strand oligonucleotides by Mre11 endonuclease activity has been implicated in ATM activation by the ... "Mre11-Rad50-Nbs1-dependent processing of DNA breaks generates oligonucleotides that stimulate ATM activity". The EMBO Journal. ...
Oligonucleotides are readily biotinylated in the course of oligonucleotide synthesis by the phosphoramidite method using ... biotinylated oligonucleotides". Tetrahedron Letters. 32 (14): 1715-8. doi:10.1016/S0040-4039(00)74311-5. Hofmann, Klaus; Wood, ...
Cell engineering methods including fluorogenic oligonucleotide signaling probes may be used to detect and isolate clonal cell ... May 2021). "Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry". Biotechnology ...
The OB-fold is a small structural motif originally named for its oligonucleotide/oligosaccharide binding properties. OB-fold ... Flynn RL, Zou L (August 2010). "Oligonucleotide/oligosaccharide-binding fold proteins: a growing family of genome guardians". ...
March 2021). "Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry". Biotechnology ... March 2021). "Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry". Biotechnology ... March 2021). "Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry". Biotechnology ...
This non-coding RNA was originally identified in E.coli using high-density oligonucleotide probe arrays (microarray.) The ... "Transcriptome analysis of Escherichia coli using high-density oligonucleotide probe arrays". Nucleic Acids Res. 30 (17): 3732- ...
Oligonucleotide design: primer design for polymerase chain reaction (PCR), probe design for fluorescence in situ hybridization ... Noguera DR, Wright ES, Camejo P, Yilmaz LS (2014). "Mathematical tools to optimize the design of oligonucleotide probes and ...
"Transcriptome analysis of Escherichia coli using high-density oligonucleotide probe arrays". Nucleic Acids Research. 30 (17): ...
"Transcriptome analysis of Escherichia coli using high-density oligonucleotide probe arrays". Nucleic Acids Research. 30 (17): ...
March 2021). "Cell engineering method using fluorogenic oligonucleotide signaling probes and flow cytometry". Biotechnology ...
The use of antisense oligonucleotides to up-regulate the expression of SynGAP protein is currently being researched. The use of ... July 2020). "Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression". Nature ... Development of antisense oligonucleotides for SYNGAP1 haploinsufficiency associated with autism spectrum disorder and ...