Nucleic Acids Research. 26 (2): 525-31. doi:10.1093/nar/26.2.525. PMC 147278. PMID 9421510. Taylor MR, Conrad JA, Wahl D, ... January 1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and ... and C-terminal oligonucleotide / oligosaccharide binding (OB) domains. Although the N-terminus of the peptide has no catalytic ...

Norm of reaction Normal distribution Northern analysis Northern blot NPD Nu body Nuclear transfer Nuclease Nucleic acid ... Fluorescent in situ hybridization) Fitness (W) Fixed allele Fixed breakage point Flow cytometry Flow karyotyping Fluctuation ... allele Null hypothesis Nullisomic Numerator element Nurse cell Ochre codon Okazaki fragment Oligo Oligogenic Oligonucleotide ... Alternative splicing Altruism Alu family Alzheimer's disease Amber codon Ames test Amino acid Amino acid sequence Amino acids ...

This is accomplished by sequential hybridization to the barcode oligonucleotide sequence of each bead. Once the location of ... Nucleic Acids Research. 33 (8): e70. doi:10.1093/nar/gni069. PMC 1087788. PMID 15860767. Ali MM, Li F, Zhang Z, Zhang K, Kang ... Nucleic Acids Research. 46 (4): e22. doi:10.1093/nar/gkx1206. PMC 5829746. PMID 29190363. Femino AM, Fay FS, Fogarty K, Singer ... in situ hybridization was developed in the late 60's and saw major developments in the 80's (smFISH) and 10's (RNAscope, ...

2008). "Genome-wide identification of in vivo protein-DNA binding sites from ChIP-seq data". Nucleic Acids Res. 36 (16): 5221- ... Oligonucleotide adaptors are then added to the small stretches of DNA that were bound to the protein of interest to enable ... The ChIP wet lab protocol contains ChIP and hybridization. There are essentially five parts to the ChIP protocol that aid in ... Nucleic Acids Research. 42 (9): e74. doi:10.1093/nar/gku178. ISSN 1362-4962. PMC 4027199. PMID 24598259. Ho, Joshua W. K.; ...

These cycles normally consist of three stages: the first, at around 95 °C, allows the separation of the nucleic acid's double ... which permits detection only after hybridization of the probe with its complementary sequence. The Minimum Information for ... sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, ... Real-time PCR can be used to quantify nucleic acids by two common methods: relative quantification and absolute quantification ...

The amplification refractory mutation system (ARMS)". Nucleic Acids Res. 17 (7): 2503-2516. doi:10.1093/nar/17.7.2503. PMC ... Because the system uses a plasmid that expresses the exo and gam genes from the λ-red system it is able to use oligonucleotide ... Shorter oligos have lower hybridization energies, resulting in decreased stability of the oligo to the chromosomal target. Of ... Nucleic Acids Research. 41 (22): e204. doi:10.1093/nar/gkt1075. ISSN 1362-4962. PMC 3905872. PMID 24203710. Sharan, Shyam K.; ...

... nucleic acid extraction, 4) reverse transcription, 5) qPCR target information, 6) oligonucleotides, 7) protocol, 8) validation ... "Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA ... This genetic disease is caused by a malfunction in the HPRT1 gene, which clinically leads to the fatal uric acid urinary stone ... TaqMan probes TaqMan probes are oligonucleotides that have a fluorescent probe attached to the 5' end and a quencher to the 3' ...

"Improved Performance of Anti-miRNA Oligonucleotides Using a Novel Non-Nucleotide Modifier". Molecular Therapy: Nucleic Acids. 2 ... This is done by hybridization of the AMOs to miRNA sequences that are involved in the expression of specific genes. The issue ... The most advanced of these compounds is miravirsen, a locked nucleic acid-DNA mixmer, currently undergoing clinical trials. An ... Davis, S.; Lollo, B; Freier, S; Esau, C (28 April 2006). "Improved targeting of miRNA with antisense oligonucleotides". Nucleic ...

"Molecular structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid", Nature, vol. 171, no. 4356, pp 737-738 Crick ... Hugo de Vries tried to link the new genetics with evolution; building on his work with heredity and hybridization, he proposed ... DNA sequencing methods improved greatly (pioneered by Frederick Sanger and Walter Gilbert), as did oligonucleotide synthesis ... it was clear to most biologists that nucleic acid sequence must somehow determine amino acid sequence in proteins; physicist ...

Nucleic Acids Res. 37 (1): e5. doi:10.1093/nar/gkn906. PMC 2615607. PMID 19015154. Schadt EE, Turner S, Kasarskis A (October ... as well as the presence of mismatches between the oligonucleotide and the hairpin. Hybridization is one way to determine the ... In the case of magnetic sequencing by hybridization, a set of overlapping tiles is used such that the sequence of each ... 7-nt primer library, 5′-NNNNNNrX-3′, are used in the ligation of a short degenerate oligonucleotide fragmentin, in which N ...

In: Nucleic Acids Research. 13, 1985, S. 1399. PMID 4000939. PMC 341080. Evans HH, Evans TE (December 1970). "Methylation of ... The adenine methyltransferase methylates the oligonucleotide making it a substrate for DpnI. Cutting of the oligonucleotide by ... Illumina Methylation Assay measures locus-specific DNA methylation using array hybridization. Bisulfite-treated DNA is ... May 2011). "QDMR: a quantitative method for identification of differentially methylated regions by entropy". Nucleic Acids ...

Sim AY, Minary P, Levitt M (June 2012). "Modeling nucleic acids". Current Opinion in Structural Biology. 22 (3): 273-8. doi: ... Entire genomes are involved in processes of hybridization, polyploidization and endosymbiosis that lead to rapid speciation. ... In addition to single-nucleotide polymorphism arrays identifying point mutations that cause cancer, oligonucleotide microarrays ... Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL (January 2008). "GenBank". Nucleic Acids Research. 36 (Database ...

Specifically, E. coli is labelled with a soybean peroxidase-labeled peptide nucleic acid (PNA) probes that bind to a specific ... Specific areas of the 16S rRNA in the Enterobacteriaceae genus are bound by oligonucleotide probes, which aids in monitoring ... and enumeration of Escherichia coli cells in municipal water by chemiluminescent in situ hybridization". Applied and ... They are facultative anaerobes with a capsule composed of complex acid polysaccharides that allows them to withstand drying for ...

A primer is a short single-stranded nucleic acid used by all living organisms in the initiation of DNA synthesis. A synthetic ... Oligonucleotide synthesis - the methods by which primers are manufactured Cox, Michael M. (2015). Molecular Biology: Principles ... avoiding the possibility of hybridization to a similar sequence nearby. A commonly used method for selecting a primer site is ... end of an existing nucleic acid, requiring a primer be bound to the template before DNA polymerase can begin a complementary ...

Kozomara, A.; Griffiths-Jones, S. (2010). "miRBase: integrating microRNA annotation and deep-sequencing data". Nucleic Acids ... An imaging method such as radioactive 5'-32P-labeled oligonucleotides along with a size ladder is used to identify a section of ... At the same time, the approach is hybridization independent and therefore does not require a priori sequence information. ... Next-generation sequencing eliminates the need for sequence specific hybridization probes required in DNA microarray analysis ...

In addition, due to the picogram level of the number of nucleic acids used, heavy amplification is often needed during sample ... 10X barcoded oligonucleotides ; Unique Molecular Identifier (UMI) sequence ; PolydT sequence (that enables capture of poly- ... by employing fluorescent in situ hybridization (FISH) and/or post-sequencing confirmation. The bias of MDA against high %GC ... Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation ...

Azad RK, Lawrence JG (May 2011). "Towards more robust methods of alien gene detection". Nucleic Acids Research. 39 (9): e56. ... The oligonucleotide spectrum owes much of its discriminatory power to the number of possible oligonucleotides: if n is the size ... Baroni M, Grünewald S, Moulton V, Semple C (August 2005). "Bounding the number of hybridisation events for a consistent ... Ornstein RL, Rein R (October 1978). "An optimized potential function for the calculation of nucleic acid interaction energies I ...

Nucleic Acids Research. 40 (21): 10937-49. doi:10.1093/nar/gks832. PMC 3505968. PMID 22965126. Iguchi, H; Kosaka, N; Ochiya, T ... Unlike microarrays, RNA sequencing is not constrained by factors such as oligonucleotide generation, and the number of probes ... in a dynamic range and increased sensitivity compared to methods such as RNase protection assays and dot blot hybridization. ... Nucleic Acids Research. 39 (16): 7223-33. doi:10.1093/nar/gkr254. PMC 3167594. PMID 21609964. Ozsolak, F; Platt, AR; Jones, DR ...

Antisense oligonucleotides (ASOs) are small strand single stranded oligodeoxynucleotides approximately 15-20 nucleic acids in ... The CGG trinucleotide expansion resides within the 5' untranslated region of the mRNA, which undergoes hybridization to form a ... Nucleic Acids Res. 39 (15): 1-11. doi:10.1093/nar/gkr429. PMC 3177194. PMID 21666257. McIvor EI, Polak U, Napierala M (2010). " ... Nucleic Acids Research. 48 (5): 2232-2245. doi:10.1093/nar/gkaa036. ISSN 0305-1048. PMC 7049705. PMID 31974547. Mariappan, S V ...

"Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR"[dead link]. Nucleic Acids Res, vol. 32, no. 17 ... Two oligonucleotides are used for this step (Figure 1: Adapter oligos). One of the oligonucleotides contains a 12-nucleotide ... hybridization capture (where used) or DNA sequencing itself can still be distinguished as a true variant. The duplex sequencing ... Nucleic Acids Res., vol. 41, no. 6, pp. 1-12, 2013. PMID 23303777. M. W. Schmitt, E. J. Fox, M. J. Prindle, et al. "Sequencing ...

A Caltech group is working on the manufacturing of these nucleic-acid-based integrated circuits. One of these chips can compute ... The DNAzyme logic gate changes its structure when it binds to a matching oligonucleotide and the fluorogenic substrate it is ... Bui, Hieu; Shah, Shalin; Mokhtar, Reem; Song, Tianqi; Garg, Sudhanshu; Reif, John (2018-01-25). "Localized DNA Hybridization ... Seelig, G.; Soloveichik, D.; Zhang, D. Y.; Winfree, E. (8 December 2006). "Enzyme-free nucleic acid logic circuits" (PDF). ...

Nucleic Acids Research. 37 (5): 1682-9. doi:10.1093/nar/gkn1042. PMC 2655659. PMID 19155277. Hu, J; Ng, PC (9 February 2012). " ... Antisense-oligonucleotide mediated exon skipping is another possibility for Duchenne muscular dystrophy. This process allows ... Studies on the effects of the length of the primer strand reveal that an equilibrium mixture of four hybridization ... The amino acid sequence encoded by the gene is then predicted. Despite the rules that govern the genetic code and the various ...

January 2018). "Xenbase: a genomic, epigenomic and transcriptomic model organism database". Nucleic Acids Research. 46 (D1): ... DNA oligonucleotides complementary to specific mRNA molecules are often chemically modified to improve their stability in vivo ... as different Xenopus species form a ploidy series formed by interspecific hybridization. In 1931, Lancelot Hogben noted that ... The expression of genes can be reduced by a variety of means, for example by using antisense oligonucleotides targeting ...

Nucleic Acids Research. 35 (1): 1-10. doi:10.1093/nar/gkl483. ISSN 0305-1048. PMC 1802572. PMID 16920744. Goodwin, Sara; ... The hybridization was successful for the ten positive samples, while the other samples resulted negative for the ancient DNA. ... or through custom design and synthesis of oligonucleotides. This method is useful when the microorganism to target is known, ... This approach is based on in solution target-probe hybridization to screen for only a single microorganism, after the ...

Benjamini Y, Speed TP (May 2012). "Summarizing and correcting the GC content bias in high-throughput sequencing". Nucleic Acids ... Short Oligonucleotide Analysis Package (SOAP) GNUMAP performs alignment using a probabilistic Needleman-Wunsch algorithm. This ... CNVseq detects copy number variations supported on a statistical model derived from array-comparative genomic hybridization. ... July 2011). "Sequence-specific error profile of Illumina sequencers". Nucleic Acids Research. 39 (13): e90. doi:10.1093/nar/ ...

According to the samples and analysis, the Icelandic samples contained about 87 distinct 16S ribosomal nucleic acid sequences, ... Christensen H, Angen O, Mutters R, Olsen JE, Bisgaard M (May 2000). "DNA-DNA hybridization determined in micro-wells using ... "Characteristic archaebacterial 16S rRNA oligonucleotides". Systematic and Applied Microbiology. 7 (2-3): 194-197. doi:10.1016/ ... Keswani J, Whitman WB (March 2001). "Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes". ...

Nucleic Acids Research. 11 (49): 1-14. doi:10.1093/nar/gkaa1112. PMC 7797060. PMID 33275144. Becquart, C; Stulz, R; Thomen, A; ... visualized the distribution of therapeutic antisense oligonucleotides labelled with bromine (Br-ASO) in some varieties of ... such as taxonomic information through fluorescence in situ hybridization (FISH) or identification of additional physiological ... "High-resolution visualization and quantification of nucleic acid-based therapeutics in cells and tissues using Nanoscale ...

The nucleic acids themselves are then synthesized using standard oligonucleotide synthesis methods, usually automated in an ... DNA machines: Yan H, Zhang X, Shen Z, Seeman NC (January 2002). "A robust DNA mechanical device controlled by hybridization ... Nucleic acid design is the process of assigning a specific nucleic acid base sequence to each of a structure's constituent ... Nucleic acid structures can be made to incorporate molecules other than nucleic acids, sometimes called heteroelements, ...

Nucleic Acids Res. 40, D351 - 361 (2012). Fortelny N., Yang S., Pavlidis P., Lange P.F., Overall C.M.. Proteome TopFIND 3.0 ... Biol.622:175-93 (2010). Kappelhoff R., Overall C.M. The CLIP-CHIP oligonucleotide microarray: dedicated array for analysis of ... its limitations of cross hybridization and dynamic range issues suggest RNA-seq will take a larger role as costs decrease and ... Nucleic Acids Res. 43(Database issue):D290-7 (2015). Doucet A., Overall C.M. Protease proteomics: revealing protease in vivo ...

2021 update". Nucleic Acids Research. 50 (D1): D231-D235. doi:10.1093/nar/gkab1083. PMC 8728126. PMID 34893873. Zhang, Ping; ... The chimeric oligonucleotide serves as a guide to allow RNase H to cleave the RNA strand precisely at the 5'-end of the ... cannot be detected using standard sequencing or hybridization methods. This modification is marked by the methylation of the ... 2008). "RNA cytosine methylation analysis by bisulphite sequencing". Nucleic Acids Research. 37 (2): e12. doi:10.1093/nar/ ...