"Base Pairing Properties of D- and L-Cyclohexene Nucleic Acids (CeNA)". Oligonucleotides. 13 (6): 479-489. doi:10.1089/ ...
Triplex-Forming Oligonucleotide Target Sequence Search Tool: A Searching Tool to find Polypurine and Polypyrimidine stretches ... Polypurine reverse-Hoogsteen hairpins (PPRHs) are non-modified oligonucleotides formed by double-stranded DNA molecules ... PPRHs are gene silencing tools acting by different mechanisms than triplex forming oligonucleotides (TFOs), antisense ... Oligonucleotides. 15: 269-83. doi:10.1089/oli.2005.15.269. PMID 16396621. de Almagro, Cristina; Coma, Silvia; Noe, Veronique; ...
Ding X, Yang J, Wang S (Mar-Apr 2011). "Antisense oligonucleotides targeting abhydrolase domain containing 2 block human ... hepatitis B virus propagation". Oligonucleotides. 21 (2): 77-84. doi:10.1089/oli.2011.0280. PMID 21466387. Human ABHD2 genome ...
Triplex-forming oligonucleotides (TFO) are one potential method to achieve therapeutic gene modulation. TFOs are approximately ... Jan 2003). "Triplex-forming oligonucleotides as modulators of gene expression". Int J Biochem Cell Biol. 35 (1): 22-31. doi: ... There are three major categories of agents that act as transcriptional gene modulators: triplex-forming oligonucleotides (TFOs ... Simon, P.; Cannata, F.; Concordet, JP.; Giovannangeli, C. (August 2008). "Targeting DNA with triplex-forming oligonucleotides ...
Oligonucleotide synthesis Antisense Antisense mRNA Morpholino Peptide nucleic acid Locked nucleic acid RNA interference (which ... This synthesized nucleic acid is termed an "anti-sense" oligonucleotide (AON) because its base sequence is complementary to the ... As of 2012, some 40 antisense oligonucleotides and siRNAs were in clinical trials, including over 20 in advanced clinical ... Over the following years, an antisense oligonucleotide later named nusinersen was developed by Ionis Pharmaceuticals under a ...
The incorporation of BNAs into oligonucleotides allows the production of modified synthetic oligonucleotides with: Equal or ... Good aqueous solubility of the resulting oligonucleotides when compared to regular DNA or RNA oligonucleotides. Chemical ... The same group, also in 2012, reported that the 2',4'-BNANC[NMe] analog when used in antisense oligonucleotides showed ... BNA nucleotides can be incorporated into DNA or RNA oligonucleotides at any desired position. Such oligomers are synthesized ...
The probe types used for non-polymorphic arrays include cDNA, BAC clones (e.g., BlueGnome), and oligonucleotides (e.g., Agilent ... Other terms used to describe the arrays used for karyotyping include SOMA (SNP oligonucleotide microarrays) and CMA (chromosome ... "Genomic analysis using high density single nucleotide polymorphism-based oligonucleotide arrays and multiplex ligation- ... "Customized oligonucleotide array-based comparative genomic hybridization as a clinical assay for genomic profiling of chronic ...
"Characteristic archaebacterial 16S rRNA oligonucleotides". Syst. Appl. Microbiol. 7: 194-197. doi:10.1016/S0723-2020(86)80005-4 ...
Many of these schemes use a covalent attachment scheme, using oligonucleotides with amide or thiol functional groups as a ... The nucleic acids themselves are then synthesized using standard oligonucleotide synthesis methods, usually automated in an ... Methods: Ellington, A.; Pollard, J. D. (1 May 2001). "Synthesis and purification of oligonucleotides". Current Protocols in ... Methods: Ellington, A.; Pollard, J. D. (1 May 2001). "Purification of oligonucleotides using denaturing polyacrylamide gel ...
"Characteristic archaebacterial 16S rRNA oligonucleotides". Syst. Appl. Microbiol. 7 (2-3): 194-197. doi:10.1016/S0723-2020(86) ...
... recognizes G-rich oligonucleotides. TLR8 is an endosomal receptor that recognizes single stranded RNA (ssRNA), and can ...
In vivo applications of antisense oligonucleotides showed that toxicity is largely due to impurities in the oligonucleotide ... Some results show that nuclear localisation signals can be irreversibly linked to one end of the oligonucleotides, forming an ... Brysch, Wolfgang; Schlingensiepen, Karl-Hermann (1994). "Design and application of antisense oligonucleotides in cell culture, ... Lebedeva, Irina; Stein, CA (2001). "Antisense oligonucleotides: promise and reality". Annual Review of Pharmacology and ...
doi:10.1016/S0040-4039(00)87907-1. Takaku, Hiroshi; Kamaike, Kazuo; Tsuchiya, Hiromichi (1984-01-01). "Oligonucleotide ...
1986). "Characteristic archaebacterial 16S rRNA oligonucleotides". Syst. Appl. Microbiol. 7 (2-3): 194-7. doi:10.1016/S0723- ...
RNase H-dependent oligonucleotides cause the target mRNA molecules to be degraded, while steric-blocker oligonucleotides ... Antisense oligonucleotides were discovered in 1978 by Paul Zamecnik and Mary Stephenson. Oligonucleotides, which are short ... The antisense oligonucleotides can affect gene expression in two ways: by using an RNase H-dependent mechanism or by using a ... Ribozymes, antisense oligonucleotides, and more recently RNAi have been used to target mRNA molecules involved in asthma. These ...
... mediated methods and oligonucleotides containing the desired mutation. These methods are most useful in organisms with ... "In vivo site-directed mutagenesis using oligonucleotides". Nature Biotechnology. 19 (8): 773-776. doi:10.1038/90837. Capecchi, ...
"Modified oligonucleotides: synthesis and strategy for users". Annu. Rev. Biochem. 67: 99-134. doi:10.1146/annurev.biochem.67.1. ... of RNA biology Molecular biology Nucleic acid methods Nucleic acid structure Nucleic acid thermodynamics Oligonucleotide ...
Verma S, Eckstein F (1998). "Modified oligonucleotides: synthesis and strategy for users". Annual Review of Biochemistry. 67: ...
"Isothermal reactions for the amplification of oligonucleotides". Proceedings of the National Academy of Sciences of the United ...
... is an oligonucleotide capable of antisense interactions with mRNA of human papillomavirus. It has been investigated ... "In vitro pharmacokinetics of phosphorothioate antisense oligonucleotides". The Journal of Pharmacology and Experimental ...
Vater A; Klussmann S (January 2015). "Turning mirror-image oligonucleotides into drugs: the evolution of Spiegelmer ... Fichou Y; Férec C (2006). "The potential of oligonucleotides for therapeutic applications". Trends in Biotechnology. 24 (12): ...
ISBN 1-84110-100-1. Dias N, Stein CA (Nov 2002). "Potential roles of antisense oligonucleotides in cancer therapy. The example ... Targeted and selective Bcl-2 inhibitors currently in the clinic include: An antisense oligonucleotide drug, oblimersen (G3139 ... of Bcl-2 antisense oligonucleotides". European Journal of Pharmaceutics and Biopharmaceutics. 54 (3): 263-9. doi:10.1016/S0939- ...
Böhler, Christof; Nielsen, Peter E.; Orgel, Leslie E. (1995-08-17). "Template switching between PNA and RNA oligonucleotides". ...
An antisense oligonucleotide drug Genasense (G3139) has been developed by Genta Incorporated to target Bcl-2. An antisense DNA ... The example of Bcl-2 antisense oligonucleotides". Eur J Pharm Biopharm. 54 (3): 263-9. doi:10.1016/S0939-6411(02)00060-7. PMID ... Dias N, Stein CA (November 2002). "Potential roles of antisense oligonucleotides in cancer therapy. ...
Gene silencing can be achieved by introducing into cells a short "antisense oligonucleotide" that is complementary to an RNA ... See the section on "antisense oligonucleotides" below. DNA strand 1: antisense strand (transcribed to)→ RNA strand (sense) DNA ... If the antisense oligonucleotide contains a stretch of DNA or a DNA mimic (phosphorothioate DNA, 2'F-ANA, or others) it can ... Steric blocking antisense mechanisms often use oligonucleotides that are heavily modified. Since there is no need for RNase H ...