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*  Negative stain
... negative_staining_electron_microscopy?ev=prf_pub "Negative staining for dummies". Retrieved 2009-06-06. "Negative staining". ... This contrasts with 'positive staining', in which the actual specimen is stained. For bright field microscopy, negative ... The choice of negative stain in electron microscopy can be very important. An early study of plant viruses using negatively ... Negative staining is usually a very mild preparation method and thus does not reduce the possibility of operator infection. ...
*  Virus
When virions are coated with stain (positive staining), fine detail is obscured. Negative staining overcomes this problem by ... Negative staining of proteins. Electron Microscopy Reviews. 1990;3(1):43-72. doi:10.1016/0892-0354(90)90013-I. PMID 1715774. ... Negative-sense viral RNA is complementary to mRNA and thus must be converted to positive-sense RNA by an RNA-dependent RNA ... To increase the contrast between viruses and the background, electron-dense "stains" are used. These are solutions of salts of ...
*  Ammonium heptamolybdate
"Cryo-negative Staining". Micron 29, p. 145-160; doi:10.1016/S0968-4328(97)00068-1. De Carlo, S.; El-Bez, C.; Alvarez-RĂșa, C.; ... as a negative stain in biological electron microscopy, typically in the 3-5% (vol/vol) concentration range and in the presence ... "Negative staining", in Harris J. R. (Ed.), Electron Microscopy in Biology, Oxford University Press, Oxford. Adrian, M.; ... "Cryo-negative staining reduces electron-beam sensitivity of vitrified biological particles". J. Struct. Biol. 138, p. 216-226; ...
*  India ink
The background is stained while the organisms remain clear. This is called a negative stain. India ink, along with other stains ... Model railroaders use a mixture of India ink and isopropyl alcohol as a wood stain, graying wood to appear aged and to bring ... Microbiologists use India ink to stain a slide containing micro-organisms. ...
*  Permanent marker
... permanent markers can actually be used to do a negative stain of a bacterial sample. This means the background is stained with ... New version of the negative stain. Applied and Environmental Microbiology. 57:1858-1859. List of pen types, brands and ... These markers are generally used on hard, non-porous surfaces, because instead of staining they form a surface layer that can ... The bacteria can be seen because they are unstained (lighter) while the background is stained (darker). Ink composition ...
*  Lutibacterium
... is a genus of Gram-negative staining bacteria. It includes the hydrocarbon-degrading strain Lutibacterium ...
*  Electron microscope
Negative staining is also used for observation of nanoparticles. Cryofixation - freezing a specimen so rapidly, in liquid ... Negative stain - suspensions containing nanoparticles or fine biological material (such as viruses and bacteria) are briefly ... specimens can be stained "en bloc" before embedding and also later after sectioning. Typically thin sections are stained for ... Staining - uses heavy metals such as lead, uranium or tungsten to scatter imaging electrons and thus give contrast between ...
*  Single particle analysis
This is particularly useful in negative stain images. The algorithms make use of fast Fourier transforms (FFT), often employing ... Individual images of stained or unstained particles are very noisy, and so hard to interpret. Combining several digitized ... This low dose, as well as variations in the metal stain used (if used) means images have high noise relative to the signal ... Tilt methods are best suited to negatively stained samples, and can be used for particles that adsorb to the carbon support ...
*  Uranyl acetate
Negative staining protocols typically treat the sample with 1% to 5% aqueous solution. Uranyl acetate staining is simple and ... Uranyl acetate is extensively used as a negative stain in electron microscopy. Most procedures in electron microscopy for ... 3-566, ISBN 0-8493-0594-2 "Negative Staining" University of Oxford Uranyl polyhalides. Molecular structure of [UO2(OAsPh3)4]( ... Some biological samples are not amenable to uranyl acetate staining and, in these cases, alternative staining techniques and or ...
*  Gap junction
Structural details and further evidence for intercellular channels by freeze-fracture and negative staining". J. Cell Biol. 57 ...
*  Lamella (cell biology)
"Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy". Journal of ...
*  Tricholosporum tropicale
They have a negative staining reaction with Melzer's reagent, and feature a conspicuous hilar appendage. The basidia (spore- ...
*  Liposome
They were discovered when Bangham and R. W. Horne were testing the institute's new electron microscope by adding negative stain ... YashRoy R.C. (1990). "Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron ... Liposomes can be easily distinguished from micelles and hexagonal lipid phases by negative staining transmission electron ... "Negative Staining of Phospholipids and Their Structural Modification by Surface-Active Agents As Observed in the Electron ...
*  Uranyl sulfate
It has found use as a negative stain in microscopy and tracer in biology. The Aqueous Homogeneous Reactor experiment, ...
*  Thermotogae
Although Thermotogae species exhibit Gram-negative staining, they are bounded by a single-unit lipid membrane, hence they are ... The phylum Thermotogae is composed of Gram-negative staining, anaerobic, and mostly thermophilic and hyperthermophilic bacteria ... Gupta R.S. (2011). "Origin of diderm (Gram-negative) bacteria: antibiotic selection pressure rather than endosymbiosis likely ...
*  Nigrosin
Furthermore, negative staining with nigrosin can reveal some microorganisms that cannot be stained by regular methods. Nigrosin ... Staining Procedures Used by the Biological Stain Commission (4th ed.). Baltimore: Williams & Wilkins. p. 412. "Nigrosin stain ... small amounts of nigrosin are used for negative staining of bacteria. as well as the capsule-containing fungus Cryptococcus ... An advantage of using this method, rather than regular positive stains like methylene blue or carbol fuchsin, is that prior ...
*  ATP5E
The F1 particle is large and can be seen in the transmission electron microscope by negative staining. These are particles of 9 ...
*  ATP synthase, H+ transporting, mitochondrial F1 complex, alpha 1
The F1 particle is large and can be seen in the transmission electron microscope by negative staining. These are particles of 9 ...
*  Dynactin
The dynein/dynactin/BICD2 (DDB) complex has also been observed, by negative stain EM, on microtubules. This shows that the ...
*  Uranyl nitrate
Along with uranyl acetate it is used as a negative stain for viruses in electron microscopy; in tissue samples it stabilizes ...
*  Membrane lipid
... negative_staining_electron_microscopy?ev=prf_pub Venturi, S.; Donati, F.M.; Venturi, A.; Venturi, M. (2000). "Environmental ... Lamellar dispersion and phase separation of chloroplast membrane lipids by negative staining electron microscopy. Journal of ...
*  Lipid bilayer
Bangham, A. D.; Horne, R. W. (1964). "Negative Staining of Phospholipids and Their Structural Modification by Surface-Active ... PC is a zwitterionic headgroup, as it has a negative charge on the phosphate group and a positive charge on the amine but, ... released by gram-negative microbes, translocate bacterial signal molecules to host or target cells to carry out multiple ... "Exocytosis from gram negative bacteria for Salmonella invasion of chicken ileal epithelium". Indian Journal of Poultry Science ...
*  Virus quantification
Sample preparations involve depositing specimens onto a coated TEM grid and negative staining with an electron-opaque liquid. ... Where P(o) is the proportion of negative tubes and m is the mean number of infectious units per volume (PFU/ml), P(o) = e(-m). ... Samples are stained with two dyes, one specific for proteins and one specific for nucleic acids, and analyzed as they flow ... Mathematically, the expected PFUs would be somewhat greater than one-half the TCID50, since the negative tubes in the TCID50 ...
*  Alec Bangham
doi:10.1016/S0140-6736(10)60950-6. Bangham, A. D.; Horne, R. W. (1964). "Negative Staining of Phospholipids and Their ... Horne, R. W.; Bangham, A. D.; Whittaker, V. P. (1963). "Negatively Stained Lipoprotein Membranes". Nature. 200 (4913): 1340. ...
*  Morax-Axenfeld diplobacilli
M. lacunata became shorter and tended to lose its Gram-negative staining characteristic when left out for 5 days. It also ... Moraxella lacunata is a rod-shaped, Gram-negative, nonmotile bacterium, generally present as diploid pairs. It causes one of ...