"The Development of Site-directed Mutagenesis by Michael Smith" (PDF). Journal of Biological Chemistry. 281 (39).. ... Site-directed mutagenesis[edit]. In 1971, Clyde Hutchison and Marshall Edgell showed that it is possible to produce mutants ... Clyde A. Hutchison III is an American biochemist and microbiologist notable for his research on site-directed mutagenesis and ... Hutchison later collaborated with Michael Smith and developed a more general method of site-directed mutagenesis using a mutant ...
Analysis by site-directed mutagenesis". J. Biol. Chem. 267 (26): 18407-18412. PMID 1526981. Guest JR, Russell GC (1991). " ...
Identification by site-directed mutagenesis". J. Biol. Chem. 275 (32): 24294-303. doi:10.1074/jbc.M002437200. PMID 10827082. ...
Identification by site-directed mutagenesis". J. Biol. Chem. 268 (6): 3980-5. PMID 8095045. GGT1 protein, human at the US ... Catalytic properties, subunit structure, and localization of the gamma-glutamyl binding site on the light subunit". J. Biol. ...
Analysis using site-directed mutagenesis". J. Biol. Chem. 265: 21027-21031. PMID 2250008. Kim, W.S.; Hatsuzawa, K.; Ishizuka, Y ...
MacDonald NJ, Freije JM, Stracke ML, Manrow RE, Steeg PS (1996). "Site-directed mutagenesis of nm23-H1. Mutation of proline 96 ... and both proteins are targeted to newly formed cell adhesion sites upon integrin engagement". J. Biol. Chem. 277 (23): 20895- ...
Szasz J, Yaffe MB, Sternlicht H (1993). "Site-directed mutagenesis of alpha-tubulin. Reductive methylation studies of the Lys ... http://www.genetex.com/WebPage/Product/PromotionDetail.aspx?Type=Promotion&PromotionID=178 Venter JC (1993). "Identification of ...
Biochemical characterization and site-directed mutagenesis". The Journal of Biological Chemistry. 266 (7): 4315-21. PMID ...
This can be achieved by: site-directed mutagenesis employing either phage- or polymerase chain reaction (PCR)-mediated methods ... Storici, Francesca; Lewis, L. Kevin; Resnick, Michael A. (2001-08-01). "In vivo site-directed mutagenesis using ... Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases" Proceedings of the National Academy of ... many target sites, single genetic mutations; (ii) single target site, many genetic mutations; and (iii) many target sites, many ...
The delitto perfetto technique is also simpler compared to other methods for in vivo site-directed mutagenesis. Other methods ... Delitto perfetto (Italian: [deˈlitto perˈfɛtto]) is a genetic technique for in vivo site-directed mutagenesis in yeast. This ... 2001) In vivo site-directed mutagenesis using oligonucleotides. Nat Biotechnol. 19(8):773-6. ... 2006) The delitto perfetto approach to in vivo site-directed mutagenesis and chromosome rearrangements with synthetic ...
Site-directed mutagenesis of a critical lysine 262". The Journal of Biological Chemistry. 266 (35): 24031-7. PMID 1748675. ... The tertiary structure consists of a beta/alpha-barrel, with the coenzyme-binding site located at the carboxy-terminus end of ... identification of glycation sites". Biochemistry. 34 (4): 1433-8. doi:10.1021/bi00004a038. PMID 7827091. Robinson B, Hunsaker ... characterization of the active site pocket". Biochemistry. 34 (35): 11264-75. doi:10.1021/bi00035a036. PMID 7669785. Takahashi ...
Lounsbury KM, Schlegel B, Poncz M, Brass LF, Manning DR (1993). "Analysis of Gz alpha by site-directed mutagenesis. Sites and ... "Identification of a GTP-binding protein alpha subunit that lacks an apparent ADP-ribosylation site for pertussis toxin". Proc. ...
Another application of site directed mutagenesis is exchanging an amino acid residue far from the active site with a lysine ... Site-directed mutagenesis is a technique that has been around since the 1970s. The early days of research in this field yielded ... Site directed mutagenesis is a valuable technique that allows for the replacement of a single base in an oligonucleotide or ... Site-directed mutagenesis can be useful for many different reasons. A single base pair replacement, could change a codon, and ...
This is often investigated through site-directed mutagenesis. In addition the synthesis of a model complex can suggest the ... Defining the active site structure. A number of important active sites are still poorly defined. This includes the oxygen ... Understanding the active site function. The structure of some enzymes are very well characterized, however, the function of ... ". "Breslow Group Homepage". www.columbia.edu. Retrieved 2015-12-11. Breslow, Ronald (1995-03-01). "Biomimetic Chemistry and ...
2010). "Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological ... Through direct mutagenesis, the scientists exchanged the Asn9 for both Asp (Aspartic Acid) and Glu (Glutamic Acid). This change ... "ATP Drives Direct Photosynthetic Production of 1-butanol in Cyanobacteria". Proceedings of the National Academy of Sciences. ... The coenzymes bind to the active site of an enzyme to promote catalysis. By engineering cofactors and coenzymes, a naturally ...
1999). "Site-directed mutagenesis of restriction endonuclease HindIII". Biosci. Biotechnol. Biochem. 63 (10): 1703-7. doi: ... Despite the uncertainty concerning the structure-catalysis relationship of type II endonucleases, site-directed mutagenesis of ... As a result of the site-mutagenesis experiments previously outlined, it is thus proposed that Lys-125, Asp-123, and Asp-108 of ... Despite the lack of evidence suggesting an exact mechanism for the cleavage of DNA by HindIII, site-mutagenesis analysis ...
Site-directed mutagenesis Restriction digest DNA footprinting Kamvysselis, M. (2003). Computational molecular genomics: genes, ... Non-directed PCR-based mutagenesis can also be used; the parameters of the mutagenic PCR reaction can be adjusted to introduce ... The effects of protein interactions with each other as well as the binding sites can also be assayed in this way; candidate ... This is often necessary because one mutation cannot be guaranteed to inactivate a binding site. ...
... and site-directed mutagenesis of inhibitor-2". J. Biol. Chem. 269 (2): 944-54. PMID 8288648. Kakinoki Y, Somers J, Brautigan DL ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. doi:10.1016/ ...
His cloning vectors were also used to develop the method for oligonucleotide site-directed mutagenesis. DNA cloning, shotgun ... sequencing and site-directed mutagenesis became widely used to sequence large DNA molecules like human chromosomes and to ... "Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis". Gene. 26 (1): 101-6. doi:10.1016/0378- ... "Messing's personal website at the Waksman Institute". Waksman.rutgers.edu. Retrieved 2010-11-10. "Jo Messing, Highly cited ...
Chueh PJ, Morré DM, Morré DJ (2002). "A site-directed mutagenesis analysis of tNOX functional domains". Biochim. Biophys. Acta ...
van Koppen CJ, Nathanson NM (1991). "Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the ...
The Gibson Assembly method can also be used for site directed mutagenesis to incorporate site-specific mutations such as ... By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... No restriction site scar remains between two DNA fragments, but the region between the double strands and hanging ends is ...
Stradal TB, Troxler H, Heizmann CW, Gimona M (2000). "Mapping the zinc ligands of S100A2 by site-directed mutagenesis". J. Biol ... 1998). "Repression of the candidate tumor suppressor gene S100A2 in breast cancer is mediated by site-specific hypermethylation ...
"Cloning and site-directed mutagenesis of human ADP-ribosylarginine hydrolase". J. Biol. Chem. 268 (24): 17837-43. PMID 8349667 ... although these proteins appear to have lost the presumed active site residues. ADP-ribosylarginine hydrolase ADP-ribosyl-( ...
Westheimer et al.'s proposal was further supported by site-directed mutagenesis studies. When Lys116 was mutated to cysteine or ... The active site, consisting of residues such as Phe27, Met97, and Tyr113, is mostly hydrophobic. However, the active site does ... Further research led to the isolation of an active site peptide sequence and identification of the active site lysine, Lys115, ... Arg29 is thought to play a role in substrate binding, while Glu76 is thought to play a role in the orienting the active site ...
By using this site, you agree to the Terms of Use and Privacy Policy. Wikipedia® is a registered trademark of the Wikimedia ... Crotty S, Cameron C, Andino R (2002). "Ribavirin's antiviral mechanism of action: lethal mutagenesis?". J. Mol. Med. 80 (2): 86 ... Infection typically occurs by direct or indirect exposure to animal excrement through the respiratory or gastrointestinal ... Treatment is directed at addressing dehydration and improving symptoms.[1] The antiviral medication, ribavirin may be useful ...