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*  Molecular cloning
... or make more subtle mutations using regional mutagenesis or site-directed mutagenesis. Obtaining the molecular clone of a gene ... to replicate inside the host organism one or more unique restriction endonuclease recognition sites to serves as sites where ... In this case, one or more specific tissues are targeted by direct treatment or by removal of the tissue, addition of the ... The restriction enzyme is chosen to generate a configuration at the cleavage site that is compatible with the ends of the ...
*  Site-directed mutagenesis
Nobel Lecture on Invention of Site-Directed Mutagenesis OpenWetWare Diagram summarizing site-directed mutagenesis. ... Direct gene deletion and site-specific mutagenesis with PCR and one recyclable marker Direct gene deletion and site-specific ... Also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and ... "SDM-Assist software to design site-directed mutagenesis primers introducing "silent" restriction sites". BMC Bioinformatics. 14 ...
*  Biotin attachment domain
Analysis by site-directed mutagenesis". J. Biol. Chem. 267 (26): 18407-18412. PMID 1526981. Guest JR, Russell GC (1991). " ...
*  Prostaglandin DP1 receptor
Identification by site-directed mutagenesis". J. Biol. Chem. 275 (32): 24294-303. doi:10.1074/jbc.M002437200. PMID 10827082. ...
*  Gamma-glutamyltransferase 1
Identification by site-directed mutagenesis". J. Biol. Chem. 268 (6): 3980-5. PMID 8095045. GGT1 protein, human at the US ... Catalytic properties, subunit structure, and localization of the gamma-glutamyl binding site on the light subunit". J. Biol. ...
*  Kallikrein 13
Analysis using site-directed mutagenesis". J. Biol. Chem. 265: 21027-21031. PMID 2250008. Kim, W.S.; Hatsuzawa, K.; Ishizuka, Y ...
*  NME1
MacDonald NJ, Freije JM, Stracke ML, Manrow RE, Steeg PS (1996). "Site-directed mutagenesis of nm23-H1. Mutation of proline 96 ... and both proteins are targeted to newly formed cell adhesion sites upon integrin engagement". J. Biol. Chem. 277 (23): 20895- ...
*  TUBA1B
Szasz J, Yaffe MB, Sternlicht H (1993). "Site-directed mutagenesis of alpha-tubulin. Reductive methylation studies of the Lys ... http://www.genetex.com/WebPage/Product/PromotionDetail.aspx?Type=Promotion&PromotionID=178 Venter JC (1993). "Identification of ...
*  RAP2A
Biochemical characterization and site-directed mutagenesis". The Journal of Biological Chemistry. 266 (7): 4315-21. PMID ...
*  Genome editing
This can be achieved by: site-directed mutagenesis employing either phage- or polymerase chain reaction (PCR)-mediated methods ... Storici, Francesca; Lewis, L. Kevin; Resnick, Michael A. (2001-08-01). "In vivo site-directed mutagenesis using ... Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases" Proceedings of the National Academy of ... many target sites, single genetic mutations; (ii) single target site, many genetic mutations; and (iii) many target sites, many ...
*  Delitto perfetto
The delitto perfetto technique is also simpler compared to other methods for in vivo site-directed mutagenesis. Other methods ... Delitto perfetto (Italian: [deˈlitto perˈfɛtto]) is a genetic technique for in vivo site-directed mutagenesis in yeast. This ... 2001) In vivo site-directed mutagenesis using oligonucleotides. Nat Biotechnol. 19(8):773-6. ... 2006) The delitto perfetto approach to in vivo site-directed mutagenesis and chromosome rearrangements with synthetic ...
*  Aldo-keto reductase family 1, member A1
Site-directed mutagenesis of a critical lysine 262". The Journal of Biological Chemistry. 266 (35): 24031-7. PMID 1748675. ... The tertiary structure consists of a beta/alpha-barrel, with the coenzyme-binding site located at the carboxy-terminus end of ... identification of glycation sites". Biochemistry. 34 (4): 1433-8. doi:10.1021/bi00004a038. PMID 7827091. Robinson B, Hunsaker ... characterization of the active site pocket". Biochemistry. 34 (35): 11264-75. doi:10.1021/bi00035a036. PMID 7669785. Takahashi ...
*  GNAZ
Lounsbury KM, Schlegel B, Poncz M, Brass LF, Manning DR (1993). "Analysis of Gz alpha by site-directed mutagenesis. Sites and ... "Identification of a GTP-binding protein alpha subunit that lacks an apparent ADP-ribosylation site for pertussis toxin". Proc. ...
*  Biomolecular engineering
Another application of site directed mutagenesis is exchanging an amino acid residue far from the active site with a lysine ... Site-directed mutagenesis is a technique that has been around since the 1970s. The early days of research in this field yielded ... Site directed mutagenesis is a valuable technique that allows for the replacement of a single base in an oligonucleotide or ... Site-directed mutagenesis can be useful for many different reasons. A single base pair replacement, could change a codon, and ...
*  Enzyme mimic
This is often investigated through site-directed mutagenesis. In addition the synthesis of a model complex can suggest the ... Defining the active site structure. A number of important active sites are still poorly defined. This includes the oxygen ... Understanding the active site function. The structure of some enzymes are very well characterized, however, the function of ... ". "Breslow Group Homepage". www.columbia.edu. Retrieved 2015-12-11. Breslow, Ronald (1995-03-01). "Biomimetic Chemistry and ...
*  Cofactor engineering
2010). "Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological ... Through direct mutagenesis, the scientists exchanged the Asn9 for both Asp (Aspartic Acid) and Glu (Glutamic Acid). This change ... "ATP Drives Direct Photosynthetic Production of 1-butanol in Cyanobacteria". Proceedings of the National Academy of Sciences. ... The coenzymes bind to the active site of an enzyme to promote catalysis. By engineering cofactors and coenzymes, a naturally ...
*  HindIII
1999). "Site-directed mutagenesis of restriction endonuclease HindIII". Biosci. Biotechnol. Biochem. 63 (10): 1703-7. doi: ... Despite the uncertainty concerning the structure-catalysis relationship of type II endonucleases, site-directed mutagenesis of ... As a result of the site-mutagenesis experiments previously outlined, it is thus proposed that Lys-125, Asp-123, and Asp-108 of ... Despite the lack of evidence suggesting an exact mechanism for the cleavage of DNA by HindIII, site-mutagenesis analysis ...
*  PPP1R2
... and site-directed mutagenesis of inhibitor-2". J. Biol. Chem. 269 (2): 944-54. PMID 8288648. Kakinoki Y, Somers J, Brautigan DL ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. doi:10.1016/ ...
*  ENOX2
Chueh PJ, Morré DM, Morré DJ (2002). "A site-directed mutagenesis analysis of tNOX functional domains". Biochim. Biophys. Acta ...
*  Mutagenesis (molecular biology technique)
The site-directed approach may be done systematically in such techniques as alanine scanning mutagenesis whereby residues are ... R A Flavell; D L Sabo; E F Bandle & C Weissmann (1975). "Site-directed mutagenesis: effect of an extracistronic mutation on the ... Papworth, C., Bauer, J. C., Braman, J. and Wright, D. A. (1996). "Site-directed mutagenesis in one day with >80% efficiency". ... Willi Müller; Hans Weber; François Meyer; Charles Weissmann (1978). "Site-directed mutagenesis in DNA: Generation of point ...
*  Muscarinic acetylcholine receptor M2
van Koppen CJ, Nathanson NM (1991). "Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the ...
*  S100A2
Stradal TB, Troxler H, Heizmann CW, Gimona M (2000). "Mapping the zinc ligands of S100A2 by site-directed mutagenesis". J. Biol ... 1998). "Repression of the candidate tumor suppressor gene S100A2 in breast cancer is mediated by site-specific hypermethylation ...
*  ADP-ribosylglycohydrolase
"Cloning and site-directed mutagenesis of human ADP-ribosylarginine hydrolase". J. Biol. Chem. 268 (24): 17837-43. PMID 8349667 ... although these proteins appear to have lost the presumed active site residues. ADP-ribosylarginine hydrolase ADP-ribosyl-( ...
*  Promoter bashing
Site-directed mutagenesis Restriction digest DNA footprinting Kamvysselis, M. (2003). Computational molecular genomics: genes, ... Non-directed PCR-based mutagenesis can also be used; the parameters of the mutagenic PCR reaction can be adjusted to introduce ... The effects of protein interactions with each other as well as the binding sites can also be assayed in this way; candidate ... This is often necessary because one mutation cannot be guaranteed to inactivate a binding site. ...
*  Mitochondrial carrier
Nelson DR, Lawson JE, Klingenberg M, Douglas MG (April 1993). "Site-directed mutagenesis of the yeast mitochondrial ADP/ATP ... In this conformation, the inhibitor may bind to the ATP-binding site. Functional and structural roles for residues in the TMSs ... 2008) identified the substrate-binding sites and salt bridge networks that are important for transport. The symmetry analyses ... Kunji ER, Robinson AJ (2006-10-01). "The conserved substrate binding site of mitochondrial carriers". Biochimica et Biophysica ...
*  Phage display
Multiple cloning sites are sometimes used to ensure that the fragments are inserted in all three possible reading frames so ... Yang WP, Green K, Pinz-Sweeney S, Briones AT, Burton DR, Barbas CF (December 1995). "CDR walking mutagenesis for the affinity ... cDNA has also been analyzed using pIII via a two complementary leucine zippers system, Direct Interaction Rescue or by adding ... Databases, programs and web servers have been widely used to exclude target-unrelated peptides, characterize small molecules- ...
*  Clyde A. Hutchison III
"The Development of Site-directed Mutagenesis by Michael Smith" (PDF). Journal of Biological Chemistry. 281 (39). Hutchison, C.A ... Clyde A. Hutchison III is an American biochemist and microbiologist notable for his research on site-directed mutagenesis and ... Hutchison later collaborated with Michael Smith and developed a more general method of site-directed mutagenesis using a mutant ... Hutchison, C.A.; Phillips, S.; Edgell, M.H.; Gillham, S.; Jahnke, P.; Smith, M. (1978). "Mutagenesis at a Specific Position in ...