Myeloma cells overexpressing Sulf1 and 2 were subcutaneously injected in severe combined immunodeficient (SCID) mice. Enhanced ... deficient mice, BMP deficient mice and hypermorphic Fgfr1 and 3 mice. This provides evidence that Sulf1 and 2 is linked to HS ... Corroborating patient data were mouse tumor in vivo studies of Sulf1 overexpressing Panc-1 cells showing decreased growth, but ... Squamous cell head and neck carcinoma (SCCHN) has three cell lines lacking Sulf1 expression. Transfected-in Sulf1 expression ...

"Lidamycin shows highly potent cytotoxic to myeloma cells and inhibits tumor growth in mice". Acta Pharmacologica Sinica. 30 (7 ... It can induce apoptosis in many cancer cells and recent studies have indicated that it induces unusual DNA damage responses to ... This unique oxygen-independent mechanism suggests that C-1027 may be effective against hypoxic tumor cells. C-1027 shows ... double-strand breaks, including altering cell cycle progression and inducing chromosomal aberrations. The structure of C-1027 ...

Cell. 46 (3): 325-34. doi:10.1016/j.molcel.2012.03.007. PMC 3360964. PMID 22483617. Mann MC, Strobel S, Fleckenstein B, Kress ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Entrez Gene: ... Sequence variation in ELL2 has been associated with multiple myeloma and altered immunoglobulin levels. GRCh38: Ensembl release ... The encoded protein is a component of the superelongation complex (SEC) and drives immunoglobulin synthesis in plasma cells. ...

1983) Methylation and rearrangement of mouse intracisternal a particle genes in development aging and myeloma. Molecular and ... 1980) "Sequence organization of clones intracisternal a particle genes." Cell 21 (2):465-473. L Hoopes, A Brown, and Ru Chih C ... and molecular biology of aging cells. Huang is co-founder and Chief Scientific Advisor of Erimos Pharmacueticals, LLC of ... the thousands and there was no consensus about homology of histones between different species or even between different cells ...

The CAR T cells based on the hYP218 antibody also show effective inhibition of tumor growth in mice. The method (i.e. grafting ... The rabbit formed a myeloma-like tumor, allowing the isolation of a plasmacytoma cell line, named 240E-1. Fusion of 240E-1 ... A rabbit hybridoma is a hybrid cell line formed by the fusion of an antibody producing rabbit B cell with a cancerous B-cell ( ... However, like the early mouse myeloma lines developed in the 1970s, stability was a concern. A number of laboratories which had ...

Mouse immunoglobin synthesis in frog cell: the translation of 9-13S myeloma RNA in frog oocytes. Journal of Molecular Biology ... Thus: The foreign mRNA can reveal control mechanisms within the living cell. Foreign proteins made within a living cell provide ... The system has also been used to evaluate control systems within the living cell. This basic discovery has generated a ... Berns, A. J. M.; Kraaikamp, M. Van; Bloemendal, H.; Lane, C. D. (1972). "Calf Crystallin Synthesis in Frog Cells: The ...

... derived membrane fractions isolated from mouse myeloma cells. The key observation by César Milstein and colleagues was that ... Böhni PC, Deshaies RJ, Schekman RW (April 1988). "SEC11 is required for signal peptide processing and yeast cell growth". The ... The Journal of Cell Biology. 67 (3): 835-851. doi:10.1083/jcb.67.3.835. PMC 2111658. PMID 811671. Paetzel M, Karla A, Strynadka ... Molecular Cell. 81 (19): 3934-3948.e11. doi:10.1016/j.molcel.2021.07.031. hdl:1874/412779. PMID 34388369. S2CID 237010364. ...

... a method of producing antibodies involving human-mouse hybrid cells, or hybridomas. They fused "mouse myeloma cells secreting ... Human x mouse somatic cell hybrid clone secreting immunoglobulins of both parental types. Nature. 1973 Aug 17;244(5416):444-7. ... The hybrid cells continued secretion of mouse immunoglobulin and initiate synthesis and secretion of human immunoglobulin." The ... antibody producing cells did not survive long and the antigens that the antibodies targeted remained unknown. In 1975, Georges ...

However, APRIL-deficient mice have also been reported to possess a reduced ability to support plasma cell survival. In vitro ... At least one anti-APRIL monoclonal antibody has been announced to enter phase I clinical trials for multiple myeloma. GRCh38: ... In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Hahne M, Kataoka T ...

ARK5 is often overexpressed in multiple myeloma cell lines. ARK5 promotes tumor cell survival under regulation by Akt. ARK5 ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Suzuki A, Kusakai G ... also inhibits ARK5 and reduces proliferation of multiple myeloma and mantle cell lymphoma cell lines. NUAK1 has been shown to ... Cell. Biol. 24 (8): 3526-35. doi:10.1128/MCB.24.8.3526-3535.2004. PMC 381626. PMID 15060171. Multi-Target Drug Blocks Myeloma ...

Unfused myeloma cells cannot grow because they lack HGPRT and thus cannot replicate their DNA. Unfused spleen cells cannot grow ... The hybridomas can be grown indefinitely in a suitable cell culture medium. They can also be injected into mice (in the ... In 1975, Georges Köhler and César Milstein succeeded in making fusions of myeloma cell lines with B cells to create hybridomas ... plasma/plasmablast cells that produce antibodies specific to an antigen of interest and fusing these cells with myeloma cells. ...

In mice, AKAP4 is required for sperm development and male mice that lack AKAP4 are infertiel The fibrous sheath was not formed ... AKAP4 is not normally expressed in mRNA and protein level in MM cell line. The A-kinase anchor proteins (AKAPs) are a group of ... AKAP4 is over expressed in multiple myeloma (MM) AKAP4 has been shown to interact with: AKAP3, and PRKAR1A. GRCh38: Ensembl ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Turner RM, Johnson ...

... are typically made by fusing the spleen cells from a mouse that has been immunized with the desired antigen with myeloma cells ... However, recent advances have allowed the use of rabbit B-cells. Another way of producing monospecific antibodies are by PrESTs ... or one cell type (including one microorganism species). However, antibodies that are monospecific to a certain tissue, or all ... but monospecific antibodies may also be produced by other means than producing them from a common germ cell. Regarding ...

The IL-2 producing RPE cells eradicated peritoneal tumors in ovarian and colorectal mouse models, and computational modeling of ... and multiple myeloma, among others Human retinal pigmented epithelial (RPE) cells can also be engineered to express a cytokine ... RPE cells engineered to produce different cytokines were encapsulated in alginate-based microparticles. The encapsulated cells ... When administered with CAR-T cells, this therapy was shown to enhance T cell infiltration to control solid tumors, while ...

Mouse cells expressing this bacterial protein were resistant to pharmacological inhibition of methyl metabolism, showing no ... Adams JM, Cory S (May 1975). "Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA". Nature. 255 (5503): 28-33. ... The effects of global methylation inhibition on the circadian period in mouse cells can be prevented by ectopic expression of ... January 2013). "ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility". Molecular Cell. 49 (1 ...

"DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival". Cell. 137 (5 ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. "Entrez Gene: ... one study has found that DEPTOR is overexpressed in multiple myeloma cells and is necessary for their survival. GRCh38: Ensembl ... doi:10.1016/j.cell.2009.03.046. PMC 2758791. PMID 19446321. Laplante M, Horvat S, Festuccia WT, Birsoy K, Prevorsek Z, Efeyan A ...

... cells. Chinese hamster ovary cell Mouse myeloma lymphoblstoid (e.g. NS0 cell) Fully Human Human embryonic kidney cells (HEK-293 ... and Schneider 2 cells and Schneider 3 cells from Drosophila melanogaster cells. With this system, cells do not lyse and several ... Cell lines used for this system include: Sf9, Sf21 from Spodoptera frugiperda cells, Hi-5 from Trichoplusia ni cells, ... Cellosaurus, a database of cell lines Gene expression Single-cell protein Protein purification Precision fermentation Host cell ...

LF3 strongly inhibits this binding in vitro, in cell lines and reduced tumor growth in mouse models. It prevented replication ... GRN163L (Imetelstat) was recently started in trials to target myeloma stem cells. Wnt signaling can become independent of ... "Critical appraisal of the side population assay in stem cell and cancer stem cell research". Cell Stem Cell. 8 (2): 136-47. doi ... These resulting mesenchymal cells have properties of cancer stem cells. In breast cancer CD44+CD24−/low cells are detectable in ...

... are mouse B myeloma cells derived from a BALB/c strain isolated by M. Bruggeman and M.S. Neuberger in the ... relative humidity with a cell density of 5x105 cell per 15 ml. This cell line is usually used to express antibodies since they ... It is a stable B cell line used to express antibodies. They are a suspension cell line and are best cultured in Eagle's minimal ... "Immunoglobulin gene expression in transformed lymphoid cells". Proc. Natl. Acad. Sci. U.S.A. 80 (3): 825-9. Bibcode:1983PNAS... ...

Hybridomas developed from mouse spleen cells fused to a myeloma cell line can be developed as a source for the cDNA encoding ... Hyperdiploid cells are defined as cells with more than 50 chromosomes, while hypodiploid are defined as cells with less than 44 ... The process as a whole result in an effector cell, typically a T-cell, that can recognize a tumor cell antigen in a manner that ... are used to classify cells by lineage. Below are immunological markers associated with B cell and T cell ALL. Cytogenetic ...

A Phase 1b/2 study of JNJ-4528, a CAR-T cell therapy directed against BCMA in myeloma patients refractory to a proteasome ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Laâbi Y, Gras MP, ... "Safety and Efficacy of ALLO-715 and ALLO-647 BCMA Allogenic CAR T Cells in Adults With Relapsed or Refractory Multiple Myeloma ... TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF). Serum B- ...

For example, a study conducted on myeloma cell line of mice found H-DNA structures in Cγ2a and Cγ2b, which participate in ... One study on in vivo targeting of hematopoietic stem cells proposed a novel technique of conjugating PNA molecules with cell ... cells and in vivo in a CF mouse model which resulted in the appearance of CFTR-dependent chloride transport. Triple-stranded ... specific TFO to the Ets2 promoter sequence that down-regulated the gene expression and led to a slowing of cell growth and cell ...

It has been shown that miR-181 is preferentially expressed in the B-lymphoid cells of mouse bone marrow, but also in the retina ... which modulate the expression of proteins essential for the pathogenesis of myeloma. Xenograft studies using human MM cell ... "miR-181a is an intrinsic modulator of T cell sensitivity and selection". Cell. 129 (1): 147-61. doi:10.1016/j.cell.2007.03.008 ... miR-181a is essential for the survival of Purkinje cells and its absence leads to a slow degeneration of these cells. It has ...

"Endogenous APOBEC3B Overexpression Constitutively Generates DNA Substitutions and Deletions in Myeloma Cells". Scientific ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Jarmuz A, Chester A ... It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene along with ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ...

In multiple myeloma, there is often protein cast in the distal convoluted tubule and collecting duct of the kidneys, mainly ... Cell Biol. 4 (6): 457-461. doi:10.1038/ncb802. PMID 12021773. S2CID 11575790. Stsiapanava A, Xu C, Brunati M, Zamora-Caballero ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Jeanpierre C, ... This is known as myeloma cast nephropathy. The glycoprotein was first purified in 1952 by Igor Tamm and Frank Horsfall from the ...

... was substantially reduced by promoter methylation in a cell line derived from multiple myeloma. and reduced by promoter ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. van der Spek PJ, ... in interleukin-6-responsive multiple myeloma KAS-6/1 cells". The Journal of Biological Chemistry. 280 (6): 4182-7. doi:10.1074/ ... cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3- ...

The surface antigen CD319 (SLAMF7) is a robust marker of normal plasma cells and malignant plasma cells in multiple myeloma. In ... Tovar V, del Valle J, Zapater N, Martin M, Romero X, Pizcueta P, Bosch J, Terhorst C, Engel P (Sep 2002). "Mouse novel Ly9: a ... "Robust isolation of malignant plasma cells in multiple myeloma". Blood. 123 (9): 1336-40. doi:10.1182/blood-2013-09-529800. ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Murphy JJ, Hobby P ...

... caring for patients receiving experimental cancer chemotherapy and research on recently discovered plasma-cell tumors in mice, ... Struck by how little was known about cancer biology, he became interested in protein synthesis in myeloma tumors, and published ...

... multiple myeloma, testicular cancer, renal carcinoma, colon carcinoma and glioblastoma multiforme. In innate immunity cells ... In mice CD200 gene is on chromosome 16. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two ... These cells can thus interact with cells expressing CD200R such as T regulatory cells, tumor-associated dendritic cells, tumor ... e127 protein is expressed on the surface of infected cells. CD200 is overexpressed in cancer cells in a number of human tumors ...

Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and ... The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic ... Cell. Endocrinol. 166 (2): 89-99. doi:10.1016/S0303-7207(00)00277-X. PMID 10996427. S2CID 45725648. Zhu M, John S, Berg M, ... "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Hou J, Schindler U ...

"Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium". In Vitro ... Clones from that cell line were isolated and a nonsecreting cell line was identified and was named NS0/1. As myeloma cells, NSo ... NS0 cells are a model cell line derived from the nonsecreting murine myeloma used in biomedical research and commercially in ... From this tumor, the P3K cells were isolated and developed into two cell lines, 289-16 and P3-X63. The 289-16 cell line ...

... a type of white blood cell cancer. It is a chimeric version of the mouse monoclonal antibody K-1-21. MDX-1097 targets kappa ... "Free Ig light chains interact with sphingomyelin and are found on the surface of myeloma plasma cells in an aggregated form". ... free immunoglobulin light chains which are found on the surface of some kappa light chain-restricted myeloma cells. MDX-1097 ... is a monoclonal antibody therapy being assessed in Phase IIb clinical trials as a treatment for multiple myeloma, ...

... among them multiple myeloma and non-small and small cell lung carcinoma cell lines. In vivo activity against human solid tumors ... in mice was shown against malignant gynecological tumor cells, like ovarian cancer, and against breast cancer. In vivo ... small cell lung carcinoma cell lines exhibit cell type-specific sensitivity to edelfosine-induced cell death and different cell ... In many tumor cells, it causes selective apoptosis, sparing healthy cells. Edelfosine can activate the Fas/CD95 cell death ...