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*  Evanescent field
In microscopy, systems that capture the information contained in evanescent waves can be used to create super-resolution images ... A total internal reflection fluorescence microscope uses the evanescent wave produced by total internal reflection to excite ... and to illuminate very small objects such as biological cells or single protein and DNA molecules for microscopy (as in the ... as in the total internal reflection fluorescence microscope) or (2) those in which the evanescent wave couples two media in ...
*  Light sheet fluorescence microscopy
... (LSFM) is a fluorescence microscopy technique with an intermediate-to-high optical ... The first application of this illumination scheme for fluorescence microscopy was published in 1993 by Voie et al. under the ... This introduced an illumination scheme into fluorescence microscopy, which has already been used successfully for dark field ... Starting in 1994, LSFM was developed as orthogonal plane fluorescence optical sectioning microscopy or tomography (OPFOS) ...
*  Supercritical angle fluorescence microscopy
... (SAF) is a technique to detect and characterize fluorescent species (proteins, ... The principle how SAF Microscopy works is as follows: A fluorescent specimen does not emit fluorescence isotropically when it ... Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy, Optic Letters 29, 569-571 (2004) ... "Novel disposable biochip platform employing supercritical angle fluorescence for enhanced fluorescence collection". Biomedical ...
*  Fluorescence-lifetime imaging microscopy
... or FLIM is an imaging technique for producing an image based on the differences in the ... Chang, CW; Sud, D; Mycek, MA (2007). "Fluorescence lifetime imaging microscopy". Methods in cell biology. Methods in Cell ... Becker, Wolfgang; Bergmann, Axel (2003). "Lifetime Imaging Techniques for Optical Microscopy" (PDF). p. 4. Fluorescence Excited ... Phasor approach to fluorescence lifetime and spectral imaging Joseph R. Lakowicz. Principles of Fluorescence Spectroscopy 3rd ...
*  Fluorescence interference contrast microscopy
Fluorescence interference contrast (FLIC) microscopy is a microscopic technique developed to achieve z-resolution on the ... After a fluorescence image is captured, each 9 or 16 terrace block yields a separate FLIC curve that defines a unique d f {\ ... The observed fluorescence intensity, I F L I C {\displaystyle I_{FLIC}} , is the product of the excitation probability per unit ... A ratio of measured fluorescence intensities at different oxide thicknesses is compared to the predicted ratio to calculate the ...
*  Fluorescence intensity decay shape microscopy
... (FIDSAM) is a fluorescence microscopy technique, which utilizes the time ... Typically, the FIDSAM technique is applied to a microscopy imaging. Whereas the measurement protocol equals fluorescence ... Accordingly, they obey the same fluorescence emission statistics and the time evolution of the fluorescence emission after a ... the initial fluorescence intensity after the excitation and τ {\displaystyle \tau } = the decay constant (fluorescence lifetime ...
*  Stokes shift
ISBN 0-470-05818-8. Rost, F. W. D. (1992). Fluorescence Microscopy. Cambridge University Press. p. 22. ISBN 0-521-23641-X. ... Stokes fluorescence is the emission of a longer-wavelength photon (lower frequency or energy) by a molecule that has absorbed a ... This number varies over several orders of magnitude, depending on the sample, and is known as the fluorescence lifetime of the ... Principles of Fluorescence Spectroscopy, Plenum Press, New York. ISBN 0-387-31278-1. Guilbault, G.G. 1990. Practical ...
*  Green fluorescent protein
The availability of GFP and its derivatives has thoroughly redefined fluorescence microscopy and the way it is used in cell ... This has triggered the development of highly automated live-cell fluorescence microscopy systems, which can be used to observe ... Yuste R (Dec 2005). "Fluorescence microscopy today". Nature Methods. 2 (12): 902-4. doi:10.1038/nmeth1205-902. PMID 16299474. ... Zn(II) binding increases fluorescence intensity, while Cu(II) binding quenches fluorescence and shifts the absorbance maximum ...
*  Protein
Yuste R (2005). "Fluorescence microscopy today". Nature Methods. 2 (12): 902-904. doi:10.1038/nmeth1205-902. PMID 16299474. ... Cryoelectron microscopy is used to produce lower-resolution structural information about very large protein complexes, ... This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The ... ISBN 0-521-65873-X. Mayhew TM, Lucocq JM (2008). "Developments in cell biology for quantitative immunoelectron microscopy based ...
*  Immunofluorescence
... microscopy, saturated structured-illumination microscopy (SSIM), fluorescence photoactivation localization microscopy (FPALM), ... extraneous undesired specific fluorescence, and nonspecific fluorescence. Autofluorescence includes fluorescence emitted from ... Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on ... Leung, Bonnie O.; Chou, Keng C. (2011-09-01). "Review of Super-Resolution Fluorescence Microscopy for Biology". Applied ...
*  Calcium concentration microdomains
... can be visualised with fluorescence microscopy by using aequorin as a reporter protein. The ... Axelrod, D. (2008). Total Internal Reflection Fluorescence Microscopy. In J. J. Correia & H. W. Detrich (Eds.), Biophysical ... Dense core granule exocytosis viewed by total internal reflection fluorescence microscopy". Methods. 46 (3): 233-238. doi: ... microdomains at the uropod of living morphologically polarized human neutrophils using flash lamp-based fluorescence microscopy ...
*  Second-harmonic imaging microscopy
SHG does not involve the excitation of molecules like other techniques such as fluorescence microscopy therefore, the molecules ... Juan Carlos Stockert, Alfonso Blázquez-Castro (2017). "Chapter 19 Non-Linear Optics". Fluorescence Microscopy in Life Sciences ... Second-harmonic imaging microscopy (SHIM) is based on a nonlinear optical effect known as second-harmonic generation (SHG). ... While two-photon-excited fluorescence (TPEF) is also a two photon process, TPEF loses some energy during the relaxation of the ...
*  Fluorophore
Juan Carlos Stockert, Alfonso Blázquez-Castro (2017). "Chapter 3 Dyes and Fluorochromes". Fluorescence Microscopy in Life ... fluorescence)) or to relay their fluorescence at even longer wavelength (see article FRET) See more on fluorescence principle. ... Tsien RY; Waggoner A (1995). "Fluorophores for confocal microscopy". In Pawley JB. Handbook of biological confocal microscopy. ... Fluorescence Microscopy in Life Sciences. Bentham Science Publishers. pp. 96-134. ISBN 978-1-68108-519-7. Retrieved 24 December ...
*  Colocalization
In fluorescence microscopy, colocalization refers to observation of the spatial overlap between two (or more) different ... Colocalization is used in real-time single-molecule fluorescence microscopy to detect interactions between fluorescently ... Curr Protoc Cell Biol "Quantitative colocalization analysis of confocal fluorescence microscopy images." Pawley JB (2006). ... 2013). "Bridging the gap between qualitative and quantitative colocalization results in fluorescence microscopy studies". Sci ...
*  Quantum dot
A QD-LED integrated at a scanning microscopy tip was used to demonstrate fluorescence near-field scanning optical microscopy ( ... Juan Carlos Stockert, Alfonso Blázquez-Castro (2017). "Chapter 18 Luminescent Solid-State Markers". Fluorescence Microscopy in ... This is called fluorescence. In a simplified model, the energy of the emitted photon can be understood as the sum of the band ... To improve fluorescence quantum yield, quantum dots can be made with "shells" of a larger bandgap semiconductor material around ...
*  Peter K. Hepler
Hepler, P. K.; B. E. S. Gunning (1998). "Confocal fluorescence microscopy of plant cells". Protoplasma. 201 (3): 121-157. doi: ... Building on the work of Shinya Inoué and Andrew Bajer using polarized light microscopy, Hepler used electron microscopy to ... Zeiger, E.; P. K. Hepler (1979). "Blue light-induced, intrinsic vacuolar fluorescence in onion guard cells". Journal of Cell ... CS1 maint: Multiple names: authors list (link) "Poems and Quotations About the MicroWorld". Microscopy Society of America. ...
*  Two-photon circular dichroism
2012). "Circular Dichroism Probed by Two-Photon Fluorescence Microscopy in Enantiopure Chiral Polyfluorene Thin Films". J. Am. ... Denk, W.; Strickler, J.; Webb, W. (1990). "Two-Photon Laser Scanning Fluorescence Microscopy". Science. 248 (4951): 73-76. ... The first experimental measurement of TPCD was performed in 1995 using a fluorescence based technique (FD-TPCD), but it was not ... Gunde, K.E.; Richardson, F.S. (1995). "Fluorescence-Detected Two-Photon Circular Dichroism of Gd3+ in Trigonal Na3[Gd(C4H4O5)3 ...
*  Watt W. Webb
Multiphoton excitation in laser scanning fluorescence microscopy provides for high resolution, high signal-to-noise imaging in ... Denk W, Strickler JH, Webb WW (1990). "Two-photon laser scanning fluorescence microscopy". Science. 248: 73-76. Bibcode:1990Sci ... Professor Webb pioneered the techniques of Fluorescence Correlation Spectroscopy (FCS) in 1972 and Multiphoton microscopy (MPM ... In situ measurements of the dynamics of fluorescence flicker by FCS, photobleaching, phototoxicity, and induced fluorescence ...
*  Two-state trajectory
Nie, S; Chiu, D.; Zare, R. (1994). "Probing individual molecules with confocal fluorescence microscopy". Science. 266 (5187): ... Weiss, Shimon (1999). "Fluorescence Spectroscopy of Single Biomolecules". Science. 283 (5408): 1676-83. Bibcode:1999Sci... ... Chung, Inhee; Bawendi, Moungi (2004). "Relationship between single quantum-dot intermittency and fluorescence intensity decays ... Zumofen, Gert; Hohlbein, Johannes; Hübner, Christian (2004). "Recurrence and Photon Statistics in Fluorescence Fluctuation ...
*  DAPI
Outside of analytical fluorescence light microscopy DAPI is also popular for labeling of cell cultures to detect the DNA of ... It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain ... Strong fluorescence when bound to DNA led to the rapid adoption of DAPI for fluorescent staining of DNA for fluorescence ... for fluorescence microscopy DAPI is excited with ultraviolet light and is detected through a blue/cyan filter. The emission ...
*  Carboxyfluorescein succinimidyl ester
Analysis by flow cytometry and fluorescence microscopy". Journal of Immunological Methods. 133 (1): 87-97. doi:10.1016/0022- ... due to the progressive halving of CFSE fluorescence within daughter cells following each cell division. The only limitation is ... approximately 7-8 cell divisions can be identified before the CFSE fluorescence is too low to be distinguished above the ...
*  Endomicroscopy
Wilson, T., Optical sectioning in fluorescence microscopy. Journal of microscopy, 2011. 242: p. 111-6. H.H.Hopkins and N.S. ... It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have ... Polglase, A.L., W.J. Mclaren, and S.A. Skinner, A fluorescence confocal endomicroscope for in vivo microscopy of the upper- and ... Conventional, widefield microscopy is generally unsuitable for imaging thick tissue because the images are corrupted by a ...
*  Membrane fluidity
... this can be observed using fluorescence microscopy. The biological analogue, 'lipid raft', is hypothesized to exist in cell ... Fluorescence correlation spectroscopy (FCS) monitors the fluctuations in fluorescence intensity measured from a small number of ... Lateral motion of molecules within the membrane can be measured by a number of fluorescence techniques: fluorescence recovery ... In fluorescence, steady-state anisotropy of the probe can be used, in addition to the rotation correlation time of the ...
*  Dichromatic mirror
Such devices are used in fluorescence microscopy.[citation needed] Murphy, Douglas B.; Davidson, Michael W. (2012). ... Fundamentals of Light Microscopy and Electronic Imaging. John Wiley & Sons. p. 221. ...
*  Transcription factories
For example, using the traditional fluorescence microscopy approach 300 - 500 factories were found but using both confocal and ... Schermelleh L (2010). "A guide to super-resolution fluorescence microscopy". J. Cell Biol. 190: 165-175. doi:10.1083/jcb. ... GFP polymerase fluorescence experiments have shown that the inducement of transcription in Drosophila polytene nuclei leads to ... electron microscopy roughly 2100 were detected. In addition to the specialisation factories have for the type of RNA polymerase ...
*  FLIC
... an extension for the FLIC animation file format Fluorescence interference contrast microscopy, an optical technique for axial ...
*  Richard Zare
Nie, S; Chiu, DT; Zare, RN (11 November 1994). "Probing individual molecules with confocal fluorescence microscopy". Science. ... Early in his career, the question of whether laser-induced fluorescence (LIF) could be used to study aflatoxins spurred Zare to ... "Molecular fluorescence and photodissociation. Zare, Richard Neil". Harvard Library. Retrieved 28 March 2017. "About Richard N. ... Zare is well known for his research in laser chemistry, particularly the development of laser-induced fluorescence, which he ...