2-Mercaptoethanol
... (also β-mercaptoethanol, BME, 2BME, 2-ME or β-met) is the chemical compound with the formula HOCH2CH2SH. ME ... Although 2-mercaptoethanol has a higher volatility than DTT, it is more stable: 2-mercaptoethanol's half-life is more than 100 ... 2-Mercaptoethanol reacts with aldehydes and ketones to give the corresponding oxathiolanes. This makes 2-mercaptoethanol useful ... 0.26 V for 2-mercaptoethanol. 2-Mercaptoethanol is often used interchangeably with dithiothreitol (DTT) or the odorless tris(2- ...
Arginyltransferase
It has 2 cofactors: mercaptoethanol, and Cation. Soffer RL (1970). "Enzymatic modification of proteins. II. Purification and ...
Morphine 6-dehydrogenase
This enzyme participates in alkaloid biosynthesis i. This enzyme has at least one effector, Mercaptoethanol. Yamano S, Kageura ...
Widal test
2-mercaptoethanol is often added to the Widal test. This agent more easily denatures the IgM class of antibodies, so if a ...
Dithiobutylamine
Dithiothreitol (DTT) 2-Mercaptoethanol (BME) TCEP Lukesh, John C.; Palte, Michael J.; Raines, Ronald T. (2012-02-21). "A Potent ...
Dithiothreitol
Dithiobutylamine (DTBA) 2-Mercaptoethanol (BME) TCEP O'Neil MJ, ed. (2001). Merck Index : an encyclopedia of chemicals, drugs ...
Modeccin
When treated with mercaptoethanol the chains can be dissociated into two subunits, subunit A with a mass of 25-28 kDa and ... Samples were made containing sodium dodecyl sulphate, and in some cases small amounts of mercapto- ethanol. A molecular weight ... These trace-fractions are more apparent in mercaptoethanol treated protein fractions. This confirms that modeccin consists of ...
Vinyl sulfone
... has applications to protein purification, especially when linked with mercaptoethanol. Leonard, Edward C. (1970 ...
5,10-Methenyltetrahydromethanopterin hydrogenase
It can be released upon denaturation with 2-mercaptoethanol or guanidine hydrochloride. Expression of the Hmd gene in E. coli ...
Gel electrophoresis
Reducing conditions are usually maintained by the addition of beta-mercaptoethanol or dithiothreitol. For a general analysis of ...
Acetaldehyde dehydrogenase
Activity can be recovered by treatment with 2-mercaptoethanol, although not with glutathione. Metronidazole (Flagyl), which is ...
Surface plasmon resonance microscopy
A pattern of ODT-mercaptoethanol was made onto a 50 nm gold film. The gold film was obtained through thermal evaporation on a ... Jenkins and collaborators 2001, used mercaptoethanol patches surrounded by SAM composed of octadecanethiol (ODT) to study the ... ODT treatment followed by immersion in 2-mercaptoethanol solutions rendered a functionalized surface for lipid membranes ...
TCEP
... can keep the cysteines from forming di-sulfide bonds and, unlike dithiothreitol and β-mercaptoethanol, it will not react ... 2-Mercaptoethanol (BME) Dithiothreitol (DTT) Dithiobutylamine (DTBA) Ruegg, U.T & Rudinger, J. (1977). "Reductive cleavage of ... Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the ...
Ellen Heber-Katz
... emphasis on 2-mercaptoethanol". The Journal of Reproduction and Development. 60 (6): 399-405. doi:10.1262/jrd.2014-055. ISSN ...
Organophosphorus acid anhydrolase
... and beta-mercaptoethanol. OPAA is also catalytically active over a wide pH range between 6.5 and 9.5 and temperature range ...
Protein sequencing
Break any disulfide bridges in the protein with a reducing agent like 2-mercaptoethanol. A protecting group such as iodoacetic ...
3-Mercaptopropane-1,2-diol
Glycerol Mercaptoethanol Nomenclature of Organic Chemistry : IUPAC Recommendations and Preferred Names 2013 (Blue Book). ...
Disulfide
In biochemistry, thiols such as β-mercaptoethanol (β-ME) or dithiothreitol (DTT) serve as reductants; the thiol reagents are ...
GPX3
The sources of reducing power for GPx-3 in vitro include GSH, cysteine, mercaptoethanol, and dithiothreitol. There is an ...
Anti-cardiolipin antibodies
Tringali GR, Julian AJ, Halbert WM (1969). "Effect of 2-mercaptoethanol treatment on anticardiolipin reactivity in sera from ...
Heparin cofactor II
β-Mercaptoethanol is typically used for the reduction of disulfide bonds within a molecule, but the gel electrophoresis ... Further experimentation demonstrated that whether β-Mercaptoethanol is present does not affect HCII's activity in gel ...
Oxycarboxin
Ethyl 2-chloroacetoacetate is treated with 2-mercaptoethanol and base, followed by cyclisation and water removal under acidic ...
Carboxin
Ethyl 2-chloroacetoacetate is treated with 2-mercaptoethanol and base, followed by cyclisation and water removal under acidic ...
Coenzyme A
... can be regenerated from CoA disulfide and mixed CoA disulfides with reducing agents such as dithiothreitol or 2-mercaptoethanol ...
Kedarcidin
... aglycone similarly undergoes reductive cycloaromatization at comparable rates irrespective of the presence of β-mercaptoethanol ...
Zymography
... the presence of 2-mercaptoethanol or DTT), and to an extent, heating. Indeed, the separations following heating to 50 °C tend ...
Polyacrylamide gel electrophoresis
... or 2-mercaptoethanol (beta-mercaptoethanol/BME), which further denatures the proteins by reducing disulfide linkages, thus ... 161-3 2-Mercaptoethanol may also be used to disrupt the disulfide bonds found between the protein complexes, which helps ...
SDS-PAGE
For this purpose, reducing thiols such as β-mercaptoethanol (β-ME, 5% by volume), dithiothreitol (DTT, 10 millimolar) or ...
Aequorin
This has also explained the need for a thiol reagent like beta mercaptoethanol in the regeneration of the protein since such ...
BME
... an organic solvent 2-Mercaptoethanol, an antioxidant also known as β-mercaptoethanol Beaver, Meade and Englewood Railroad, a ...