Isoelectric focusing
... is the first step in two-dimensional gel electrophoresis, in which proteins are first separated by their ... A protein that is in a pH region below its isoelectric point (pI) will be positively charged and so will migrate toward the ... Proteins are introduced into an Immobilized pH gradient gel composed of polyacrylamide, starch, or agarose where a pH gradient ... Isoelectric focusing is the only step in preparative one-dimensional gel electrophoresis. According to some opinions, living ...
QPNC-PAGE
Gordon AH (1969). Electrophoresis of proteins in polyacrylamide and starch gels (Part I, Chapter 2 Acrylamide gel). pp. 34-45. ... The aim of isoelectric focusing (IEF), for example, is to separate proteins according to their isoelectric point (pI), thus, ... Electrophoresis Gel electrophoresis Agarose gel electrophoresis Protein electrophoresis Electroelution Protein purification ... This standardized variant of native gel electrophoresis and subset of preparative polyacrylamide gel electrophoresis is used by ...
List of MeSH codes (H01)
... electrophoresis, gel, two-dimensional MeSH H01.181.529.307.437.402 - starch gel electrophoresis MeSH H01.181.529.307.437.568 - ... isoelectric point MeSH H01.181.529.444 - hydrolysis MeSH H01.181.529.450 - hydrophobicity MeSH H01.181.529.479 - ion exchange ... electrophoresis, gel, pulsed-field MeSH H01.181.529.307.437.230 - electrophoresis, gel, two-dimensional MeSH H01.181.529.307. ... electrophoresis MeSH H01.181.529.307.437.100 - agar gel electrophoresis MeSH H01.181.529.307.437.100.150 - comet assay MeSH ...
Metaproteomics
The first proteomics experiment was conducted with the invention of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE ... 250, 4007-4021 (1974). Klose, J. Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel ... After subjects consumed diets high in resistant starch, it was discovered that several microbial proteins were altered such as ... for the large-scale characterization of the entire protein complement of environmental microbiota at a given point in time. At ...
List of MeSH codes (E05)
... electrophoresis, gel, two-dimensional MeSH E05.196.401.485 - electrophoresis, starch gel MeSH E05.196.401.500 - electrophoretic ... skin test end-point titration MeSH E05.196.941.336 - centrifugation, density gradient MeSH E05.196.941.336.253 - centrifugation ... isoelectric focusing MeSH E05.196.454.807 - ultrafiltration MeSH E05.196.454.807.300 - hemofiltration MeSH E05.196.454.807. ... gel, pulsed-field MeSH E05.196.401.250 - electrophoresis, gel, two-dimensional MeSH E05.196.401.319 - electrophoresis, paper ...