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*  DNA-encoded chemical library
In contrast to conventional screening procedures such as high-throughput screening, biochemical assays are not required for ... Drug discovery High-throughput screening Combinatorial chemistry DNA sequencing Phage display Smith GP (June 1985). " ... the lack of surface artifacts and the high fidelity of the yR library and screening method. Screening is performed in a single ... due both to the high cost per base for the sequencing and to the tedious procedure involved. High throughput sequencing ...
*  Antibody microarray
Antibody arrays for high-throughput screening of antibody-antigen interactions. Nature Biotechnol. 18, 989 -994 Holt L. J., ... High-throughput microarray-based enzyme-linked immunosorbent assay (ELISA). Biotechniques 27, 778 -788 Lueking A., Horn M., ... Glass slide-based arrays, owing to their smooth and rigid structure, can also be easily fitted to high-throughput liquid ... While 96-well plate-based antibody arrays have high-throughput capability, the small surface area in each well limits the ...
*  High-throughput screening
"A pair of new statistical parameters for quality control in RNA interference high-throughput screening assays". Genomics. 89 (4 ... Drug discovery hit to lead Virtual high throughput screening High-content screening High throughput biology Drug discovery Z- ... Parallel access to high-throughput small molecule screen and a genome wide screen enables researcher to perform target ... The term uHTS or ultra-high-throughput screening refers (circa 2008) to screening in excess of 100,000 compounds per day. With ...
*  Luciferase
Fan F, Wood KV (Feb 2007). "Bioluminescent assays for high-throughput screening". ASSAY and Drug Development Technologies. 5 (1 ... Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. ... The sensitivity and high signal intensity of this luciferase molecule proves advantageous in many reporter studies. Some of the ... "Introduction to Bioluminescence Assays". Promega Corporation. Retrieved 2009-03-07. Williams TM, Burlein JE, Ogden S, Kricka LJ ...
*  Cytotoxicity
Fan F, Wood KV; Wood (February 2007). "Bioluminescent assays for high-throughput screening". Assay Drug Dev Technol. 5 (1): 127 ... or they can screen "hits" from initial high-throughput drug screens for unwanted cytotoxic effects before investing in their ... Cytotoxicity can also be measured by the sulforhodamine B (SRB) assay, WST assay and clonogenic assay. Suitable assays can be ... Cytotoxicity assays are widely used by the pharmaceutical industry to screen for cytotoxicity in compound libraries. ...
*  Ligand binding assay
Sittampalam, G. S.; Kahl, S. D.; Janzen, W. P. (1997). "High-throughput screening: Advances in assay technologies". Current ... binding-assay.com/binding-assay-types/solid-phase-assays/on-bead-assays Moss, ed. by Tom (2001). "Filter-Binding Assays". DNA- ... Many ligand binding assays require a filtration step to separate bound and unbound ligands before screening. A method called ... Ligand binding assays (LBA) is an assay, or an analytic procedure, whose procedure or method relies on the binding of ligand ...
*  Anoikis
Using a novel high-throughput screening assay, Mawji et al. showed that anisomycin can sensitize metastatic epithelial cells to ... In related work, Schimmer's team showed that FLIP levels are higher in metastatic cells than non-metastatic cells, and that ... September 2007). "A chemical screen identifies anisomycin as an anoikis sensitizer that functions by decreasing FLIP protein ...
*  Guangxitoxin
"A KV2.1 gating modifier binding assay suitable for high throughput screening". Channels (Austin, Tex.). 3 (6): 437-47. doi: ... a high-affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels". Biochemistry. 49 (25): 5134-42. ...
*  Microtiter plate
Inglin, Raffael C. (2015). "High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus ... Lindström, Sara; Larsson, Rolf; Svahn, Helene Andersson (2008-03-01). "Towards high-throughput single cell/clone cultivation ... A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in ... Cyclo-olefins are now being used to provide microplates which transmit ultraviolet light for use in newly developed assays. ...
*  Lactobacillus
Inglin, Raffael C. (2015). "High-throughput screening assays for antibacterial and antifungal activities of Lactobacillus ... Many species of this genus do not require iron for growth and have an extremely high hydrogen peroxide tolerance.[citation ... The probiotics help treat IBS by returning homeostasis when the gut microbiota experiences unusually high levels of ...
*  Z-factor
"Alternative Statistical Parameter for High-Throughput Screening Assay Quality Assessment". Journal of Biomolecular Screening. ... The Z-factor is an attempt to quantify the suitability of a particular assay for use in a full-scale, high-throughput screen. ... screens are used to assess the quality of an assay, in an attempt to predict if it would be useful in a high-throughput setting ... to judge whether the response in a particular assay is large enough to warrant further attention. In high-throughput screens, ...
*  Cytopathic effect
"Development of a Robust Cytopathic Effect-Based High-Throughput Screening Assay to Identify Novel Inhibitors of Dengue Virus". ... An assay has been developed that screens the dengue virus's CPEs in order to assess cell viability. Due to the host cell ... Inoculations always occur at low multiplicity of infection because at high multiplicity of infection, all CPEs occur rapidly. ... Viral culture Indirect immunoperoxidase assay Virus "Cytopathic Effect (CPE)". Encyclopædia Britannica. Retrieved 19 November ...
*  Imidazoleglycerol-phosphate dehydratase
Cagney, G.; Uetz, P.; Fields, S. (2000). "High-throughput screening for protein-protein interactions using two-hybrid assay". ...
*  Albert P. Li
Screen. 16(8), 903-909. Li, A. P. and Doshi U. (2011) Higher Throughput Screening Assays for Time-Dependent Inhibition of ... 179(1), 4-8. Li, A. P. and Doshi U. (2011) Higher Throughput Screening Assays for CYP3A4 Inhibition and Induction in Human ... Li and his researchers have developed numerous hepatocyte-based assays, including higher throughput assays for P450 inhibition ... Notable publications include: Li A. P. (2005) Preclininical in vitro screening assays for drug-like properties. Drug Discovery ...
*  Spot analysis
Another application is a High-throughput screenings that often uses spot assays to determine the growth of eg. mated cells or ... A spot assay or spot test can also refer to a specific test in microbiology. This test is often used to check the growth rate ... A spot test or spot assay can also refer to a test often used in microbiology. The test has been known in other languages as " ... Spot analysis, spot test analysis, or spot test is a chemical test, a simple and efficient technique where analytic assays are ...
*  Strictly standardized mean difference
In high-throughput screening (HTS), quality control (QC) is critical. An important QC characteristic in an HTS assay is how ... "A pair of new statistical parameters for quality control in RNA interference high-throughput screening assays". Genomics. 89 (4 ... "A simple statistical parameter for use in evaluation and validation of high throughput screening assays". Journal of ... and t Statistic for Hit Selection in RNAi High-Throughput Screens". Journal of Biomolecular Screening. 16 (7): 775-85. doi: ...
*  C+-probability
... balanced control of false negatives and false positives for hit selection in RNA interference high-throughput screening assays ... Zhang XHD (2011). Optimal High-Throughput Screening: Practical Experimental Design and Data Analysis for Genome-scale RNAi ... The c+-probability and d+-probability have been used for data analysis in high-throughput experiments and biopharmaceutical ... ". Journal of Biomolecular Screening. 12 (5): 645-55. doi:10.1177/1087057107300645. PMID 17517904. Owen DB, Graswell KJ, Hanson ...
*  Kevin Lustig
October 2008). "A Robotic Platform for Quantitative High-Throughput Screening". Assay Drug Development Technology. 6 (5): 637- ... The company was a pioneer in using high throughput screening (HTS) for phenotypic drug screens. The company raised over $170M ... In 1996, Lustig and Marc Kirschner invented a paracrine signaling assay and used it to identify Xnr1, which is part of a cell ... "Assay Depot, Inc. Creates Medical Research Exchange With the National Cancer Institute". BioSpace. BioSpace. 6 August 2013. ...
*  Thallium poisoning
A Homogenous Thallium Flux Assay for High Throughput Screen of Potassium Channels Eliminating Thallium (poisoning) Blum, The ... Urine screening revealed elevated thallium levels in a small number of other members of the chemistry department, though none ... Part of the reason for thallium's high toxicity is that, when present in aqueous solution as the univalent thallium(I) ion (Tl+ ... The primary (and only) suspect of the police investigation, Sun Wei, is a member of a family with high-level political ...
*  Stuart Schreiber
"High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational ... MacBeath G, Schreiber SL (September 2000). "Printing proteins as microarrays for high-throughput function determination". ... Academic screening centers have been created that emulate the Harvard Institute of Chemistry and Cell Biology and the Broad ... Multidimensional screening was introduced in 2002 and has provided insights into tumorigenesis, cell polarity, and chemical ...
*  SLAS Discovery
... or high-throughput screening; lead generation/optimization; chemical biology; and informatics. The journal was published from ... This includes scientific and technical advances in target identification/validation; biomarker discovery; assay development; ... Official website Society for Laboratory Automation and Screening. ... is a peer-reviewed scientific journal published by the Society for Laboratory Automation and Screening (SLAS) in partnership ...
*  Multiplex (assay)
Multiplex assays are often used in high-throughput screening settings, where many specimens can be analyzed using a multiplex ( ... Strictly speaking, a multiplex assay is not necessarily high-throughput. When the execution of a single multiplex assay ... Massive parallelization of assays is one way to achieve "high-throughput" status. Another way is via automation of manual ... DNA microarray used for gene expression or SNP detection assays SAGE for gene expression High-throughput sequencing which can ...
*  Aurora Biosciences
... and to develop and sell new high-throughput screening (HTS) equipment and assays to use on the equipment. They were leaders in ... and Aurora became a leader in providing assay development and high throughput screening services and equipment to the pharma ... and it was recognized as the industry leader in assay development and high-throughput screening services. In 2000 Aurora ... November 04, 2011 Evolution of Ultra-High-Throughput Screening Zaheer Ul-Haq and Jeffry D. Madura. Frontiers in Computational ...
*  Compound management
J. Richard Archer (2004-12-01). "History, Evolution, and Trends in Compound Management for High Throughput Screening". ASSAY ... These chemical libraries are used in high-throughput screening in the drug discovery hit to lead process. The chemical ... It has become a critical technological component for high-throughput screening and chemical genomics. There are great ... April 2008). "Compound Management for Quantitative High-Throughput Screening" (PDF). JALA Charlottesv Va. 13: 79-89. doi: ...
*  Hit selection
... balanced control of false negatives and false positives for hit selection in RNA interference high-throughput screening assays ... Effect size high-throughput screening Z-score SSMD SMCV Dual-flashlight plot Zhang XHD (2011) "Optimal High-Throughput ... "Improved statistical methods for hit selection in high-throughput screening". Journal of Biomolecular Screening. 8: 634-47. doi ... In high-throughput screening (HTS), one of the major goals is to select compounds (including small molecules, siRNAs, shRNA, ...
*  Phage display
Like the two-hybrid system, phage display is used for the high-throughput screening of protein interactions. In the case of M13 ... pVII and pIX fusions without pelB was more efficient than pIII fusions in affinity selection assays. The paper even goes on to ... Below is the sequence of events that are followed in phage display screening to identify polypeptides that bind with high ... A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction". Eur. J. Biochem. ...
*  Chemical library
History, evolution, and trends in compound management for high-throughput screening. Assay Drug Dev Technol. 2004 Dec;2(6):675- ... In drug discovery high-throughput screening, it is desirable to screen a drug target against a selection of chemicals that try ... A chemical library or compound library is a collection of stored chemicals usually used ultimately in high-throughput screening ... a wide range of organic chemicals are needed to test against models of disease in high-throughput screening. Therefore, most of ...