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*  Green fluorescent protein
Roger Tsien on fluorescent proteins Excitation and emission spectra for various fluorescent proteins Green Fluorescent Protein ... The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green ... Phillips GJ (Oct 2001). "Green fluorescent protein--a bright idea for the study of bacterial protein localization". FEMS ... Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first ...
*  Roger Y. Tsien
... "the green fluorescent protein: discovery, expression and development." The multicolored fluorescent proteins developed in ... Typically, the gene coding for a protein of interest is fused with the gene for a fluorescent protein, which causes the protein ... "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-7. doi:10.1038/ ... "Green Fluorescent Protein: Timeline" (Web page). Conncoll.edu. 18 Nov 2009. Retrieved December 22, 2009. Xiaokun Shu; Antoine ...
*  Immunofluorescence
An alternative approach is using recombinant proteins containing fluorescent protein domains, e.g., green fluorescent protein ( ... Chalfie, Martin (1995-10-01). "Green Fluorescent Protein". Photochemistry and Photobiology. 62 (4): 651-656. doi:10.1111/j.1751 ... Likewise, an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen ... Proteins in the supernatant or on the outside of the cell membrane can be bound by the antibodies; this allows for living cells ...
*  Martin Chalfie
"Green Fluorescent Protein". The Golden Goose Award. Retrieved 2015-05-27. Chalfie's lab Website Chalfie Nobel lecture Martin ... 1994). "Green fluorescent protein as a marker for gene expression". Science. 263 (5148): 802-805. doi:10.1126/science.8303295. ... He traces his work on Green fluorescent protein to a 1988 seminar from Paul Brehm about bioluminescent organisms, which led to ... She gave him permission to cite her unpublished research in his seminal Science paper "Green Fluorescent Protein as a Marker ...
*  Biliverdin
Tsien, Roger Y. (1998-01-01). "The Green Fluorescent Protein". Annual Review of Biochemistry. 67 (1): 509-544. doi:10.1146/ ... but green blood that gives terrestrial animals a green color seems to occur nearly exclusively in species that inhabit green ... In contrast to fluorescent proteins which form their chromophore through posttranslational modifications of the polypeptide ... 2011). "Bright and stable near infra-red fluorescent protein for in vivo imaging". Nat Biotechnol. 29 (8): 757-761. doi:10.1038 ...
*  Aequoreidae
The full text Tsien, R. (1998). The green fluorescent protein. Annual Review of Biochemistry 67: 509-44. The full text. doi: ... Aequoreids include Aequorea victoria, the organism from which the green fluorescent protein gene was isolated. Only the polyp ...
*  Spinach aptamer
The aptamer was designed to be an RNA mimic of green fluorescent protein (GFP); similar to GFP for proteins, Spinach can be ... Paige, J. S.; Wu, K. Y.; Jaffrey, S. R. (2011). "RNA Mimics of Green Fluorescent Protein". Science. 333 (6042): 642-646. doi: ... As the fluorophore of GFP and its derivatives are covalently bound to/a part of the protein, free exchange cannot happen and ... Spinach has also been adapted for sensing proteins or molecules in vivo. An adapted structure, which includes two binding sites ...
*  Michael Elowitz
Elowitz, M. B.; Surette, M. G.; Wolf, P. E.; Stock, J.; Leibler, S. (1997). "Photoactivation turns green fluorescent protein ...
*  Systematic evolution of ligands by exponential enrichment
Paige, J. S.; Wu, K. Y.; Jaffrey, S. R. (2011). "RNA Mimics of Green Fluorescent Protein". Science. 333 (6042): 642-646. doi: ... Mercey, R; Lantier, I; Maurel, MC; Grosclaude, J; Lantier, F; Marc, D (2006). "Fast, reversible interaction of prion protein ... Blackwell, TK; Weintraub, H (1990). "Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes ... which may be a protein or a small organic compound - and those that do not bind the target are removed, usually by affinity ...
*  List of University of California, San Diego people
Known for discovering green fluorescent protein. Chinary Ung, Music. Grawemeyer Award winning composer. Harold Urey, Chemistry ... How a fluorescent jellyfish - and federal dollars - helped fight AIDS, The Washington Post, Retrieved 14 September 2012. UCSD ...
*  Calcium imaging
These indicators are fluorescent proteins derived from green fluorescent protein (GFP) or its variants (e.g. circularly ... "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-887. doi:10.1038/ ... Nagai, T.; Sawano, A.; Park, E. S.; Miyawaki, A. (2001-03-13). "Circularly permuted green fluorescent proteins engineered to ... "Genetically encoded indicators of cellular calcium dynamics based on troponin C and green fluorescent protein". The Journal of ...
*  Aequorin
In the animals, the protein occurs together with the Green fluorescent protein to produce green light by resonant energy ... It was also noted during the extraction the animal creates green light due to the presence of the green fluorescent protein, ... "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-87. doi:10.1038/ ... Shimomura O (2005). "The discovery of aequorin and green fluorescent protein". J Microsc. 217 (Pt 1): 1-15. doi:10.1111/j.0022- ...
*  Mikhail Matz
In 1999 his paper on cloning of six homological fluorescent proteins into green fluorescent protein of A. victoria and ... victoria jellyfish which glows bright green under ultraviolet light when green fluorescent protein was added. The study was ... Rebekka Wachter (2015). "New mechanism unlocked for evolution of green fluorescent protein". Structure. 23 (1): 34-43. doi: ... It was revealed that the common ancestor of both green and red proteins is green one. In 2006 he and Rasmus Nielsen have ...
*  Fluorescence in the life sciences
Phycoerythrin or green fluorescent protein). Alternatively, specific or general proteins, nucleic acids, lipids or small ... Several fluorescent protein exist in nature[citation needed], but the most important one as a research tool is Green ... Chalfie, M; Tu, Y; Euskirchen, G; Ward, WW; Prasher, DC (1994). "Green fluorescent protein as a marker for gene expression". ... Other proteins are fluorescent but require a fluorophore cofactor, and hence can only be used in vitro; these are often found ...
*  Live single-cell imaging
"Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin". Nature. Springer Nature. 388 (6645): 882- ... Fluorescent tagging: Here a gene encoding a fluorescent protein is inserted into the coding frame of the protein to be tagged. ... "Green fluorescent protein as a marker for gene expression." Trends in Genetics 10.5 (1994): 151. Ng, T. (1999). "Imaging ... The field of live single-cell imaging began with work demonstrating that Green Fluorescent Protein (GFP), found in the ...
*  Behavioral neuroscience
"Genetically Encoded Indicators of Cellular Calcium Dynamics Based on Troponin C and Green Fluorescent Protein." The Journal of ... "Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins." Nature 394, 192-195 (9 July 1998 ... that relies on a fusion protein that combines a synaptic vesicle membrane protein and a pH sensitive fluorescent protein. Upon ... Genetically encoded voltage sensitive fluorescent proteins have also been developed. Calcium imaging relies on dyes or ...
*  Optogenetics
"Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-887. doi:10.1038/ ... where scientists have fused fluorescent proteins to detector proteins. An example of this is voltage-sensitive fluorescent ... To identify expressing cells, they replaced the cytoplasmic tail of the algal protein with the fluorescent protein YFP, ... probe composed of a single green fluorescent protein". Nature Biotechnology. 19 (2): 137-141. doi:10.1038/84397. ISSN 1087-0156 ...
*  James Rothman
"Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins". Nature. 394 (6689): 192-195. doi ... postdoctoral research with Harvey Lodish at Massachusetts Institute of Technology working on glycosylation of membrane proteins ...
*  Yellow fluorescent protein
... (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish ... "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-7. doi:10.1038/ ... Introduction to fluorescent proteins Miyawaki, Atsushi; Llopis, Juan; Heim, Roger; McCaffery, J. Michael; Adams, Joseph A.; ... "A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications". Nature ...
*  CALM2
"Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-7. doi:10.1038/ ... interactions with melittin and microtubule-associated tau proteins and inhibition of phosphorylation of tau proteins by protein ... Calmodulin 2 is a protein that in humans is encoded by the CALM2 gene. Mutations in CALM2 are associated to cardiac arrhythmias ... Takahashi M, Yamagiwa A, Nishimura T, Mukai H, Ono Y (Sep 2002). "Centrosomal proteins CG-NAP and kendrin provide microtubule ...
*  Laser
The cells were genetically engineered to produce green fluorescent protein (GFP). The GFP is used as the laser's "gain medium ... "Green Laser 400 mW burn a box CD in 4 second". YouTube. Retrieved December 10, 2011. "Laser Diode Power Output Based on DVD-R/ ... "Green Laser". osram-os.com. August 19, 2015. "Picolight ships first 4-Gbit/s 1310-nm VCSEL transceivers". Laser Focus World ... Upon bathing the cell with blue light, it could be seen to emit directed and intense green laser light. When lasers were ...
*  Fura-2
"Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882-7. doi:10.1038/ ... More recently, genetically-encoded calcium indicators based on spectral variants of the green fluorescent protein, such as ... Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the ...
*  Gero Miesenböck
"Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins". Nature. 394 (6689): 192-195. doi ... This involved inserting DNA for light-responsive opsin proteins into the cells. Miesenböck used similar genetic modifications ...
*  Beta barrel
... is one of the few structures of this protein that is not obtained from a mutant protein. Green fluorescent protein 1RRX Eleven ... To illustrate, S will be calculated for green fluorescent protein. This protein was chosen because the beta barrel contains ... Examples of the family include retinol binding proteins (RBPs) and major urinary proteins (Mups). RBP binds and transports ... see low-frequency collective motion in proteins and DNA. Wimley, WC. (2003). "The versatile beta-barrel membrane protein". Curr ...
*  Fluorescent tag
Green fluorescent protein is a naturally occurring fluorescent protein from the jellyfish Aequorea victoria that is widely used ... YFP or yellow fluorescent protein, BFP or blue fluorescent protein, and CFP or cyan fluorescent protein are examples of GFP ... fluorescent proteins or FPs were introduced. Green fluorescent protein or GFP was discovered by Osamu Shimomura in the 1960s ... Alterations of fluorescent proteins would lead to loss of fluorescent properties. Protein labeling uses a short tag to minimize ...
*  Anterograde tracing
Green fluorescent protein, lipophylic dyes or radioactively tagged amino acids) into the brain. These molecules are absorbed ... There is also a group of tracers that consist of protein products that can be taken up by the cell and transported across the ... virus or protein can be locally injected, after which it is allowed to be transported anterogradely. Viral tracers can cross ...