Medical microbiology
A major milestone in medical microbiology is the Gram stain. In 1884 Hans Christian Gram developed the method of staining ... can be performed immediately after the sample is taken from the patient and is used in conjunction with biochemical staining ...
Akkermansia
... non-motile and stain Gram-negative. Strictly anaerobic organism. Chemo-organotrophic. Mucolytic in pure culture.: 1474 The ...
Methanothermus fervidus
The cells are motile, strictly anaerobic and stain Gram positive. They can grow at temperatures as high as 97 °C. Strain V24ST ...
Deinococcus aerius
A gram stain revealed that these bacteria are gram-positive. Scanning electron microscopy revealed that cells of this bacterium ...
Sphaerotilus natans
Gram and Neisser staining reactions are negative. S. natans requires dissolved simple sugars or organic acids as a food supply ...
Legionella jordanis
The two strains were both Gram-stained. The Sudan black B fat stain for lipids and the Wirtz-Conklin method were used to ... Strains of L. jordanis are thin, motile Gram-negative rods that range in size from 0.3 to 0.9 µm wide by 2 to 20 µm long. In ... Acid-fast staining was used, as well. The cultures were streaked onto trypticase soy agar (TSA) and charcoal yeast extract (CYE ... After being stained with Sudan B, many of the cells did not have fat deposits. Gas-liquid chromatography-mass spectrometry show ...
Ziehl-Neelsen stain
These acids resist staining by ordinary methods such as a Gram stain. It can also be used to stain a few other bacteria, such ... rendering them resistant to conventional staining techniques like the Gram stain. After the Ziehl-Neelsen staining procedure ... Lowenstein-Jensen medium Gram stain Kinyoun stain Acid-fastness Franz Ziehl Friedrich Neelsen Talbot, Elizabeth A.; Raffa, ... The Ziehl-Neelsen stain is a two step staining process. In the first step, the tissue is stained with a basic fuchsin solution ...
Orbital cellulitis
Staphylococcus infections are identified by a cluster arrangement on gram stain. Staphylococcus aureus forms large yellow ... Staphylococcus aureus is a gram-positive bacterium, which is the most common cause of staphylococcal infections. Staphylococcus ... Streptococcus pneumoniae is also a gram-positive bacterium responsible for orbital cellulitis due to its ability to infect the ...
Desulfobacteraceae
They stain Gram-negative and are not known to produce spores. Some species contain a single polar flagellum used for motility. ...
Bile esculin agar
Test results must be interpreted in conjunction with gram stain morphology. Bile Esculin Agar is used primarily to ...
Filobacillus milensis
Its cells are motile, strictly aerobic rods which stain Gram-negative. Its type strain is SH 714T (= DSM 13259T = ATCC 700960T ...
Mycobacterium lepromatosis
This prohibits the standard use of the Gram stain in characterization. "Mycobacterium lepromatosis Han et al. 2008, effective ... Members of the Mycobacterium genus are characterized by being Gram-positive, non-motile, non-spore-forming, and possess a ...
Bacterial cell structure
... those of gram-positive bacteria and those of gram-negative bacteria, which are differentiated by their Gram staining ... The gram-positive bacteria take up the crystal violet dye and are stained purple. The cell wall of some gram-positive bacteria ... Gram-negative bacteria are stained as pink colour. The chemical structure of the outer membrane's lipopolysaccharide is often ... Gram-negative cell walls are much thinner than the gram-positive cell walls, and they contain a second plasma membrane ...
Alicyclobacillus cellulosilyticus
The bacteria Gram stains negative and produce endospores. It was first "isolated from steamed Japanese cedar chips from a ...
Bacterial vaginosis
An alternative is to use a Gram-stained vaginal smear, with the Hay/Ison criteria or the Nugent criteria. The Hay/Ison criteria ... DNA hybridization testing with Affirm VPIII was compared to the Gram stain using the Nugent criteria. The Affirm VPIII test may ... Ison CA, Hay PE (December 2002). "Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary ... These swabs can be tested for: Gram stain which shows the depletion of lactobacilli and overgrowth of Gardnerella vaginalis ...
Carl Friedländer
... the Gram Stain) to see the bacteria because using ordinary stains (H&E) nuclei and fibrin stained the same way as bacteria and ... Zentralblatt für die medizinischen Wissenschaften, Berlin, 1876, 14 Austrian, R. (1960). "The Gram stain and the etiology of ... Friedländer also noted that it was necessary to use special stains (e.g. ...
Richard F. Edlich
This reliable Gram stain technique is now being used throughout the world. When Edlich treated burn patients in which the ... Edlich devised a new Gram stain technique for quantitative bacteriology using stable iodophors rather than unstable aqueous ...
Methanosarcina barkeri
Methanosarcina could produce positive Gram stain, but generally, it is Gram variable. M. barkeri has a thick cell wall ...
Acidovorax facilis
They are negative by Gram stain and positive by the oxidase test. When grown on nutrient agar, they form unpigmented colonies. ...
Group A streptococcal infection
Gram-negative bacteria
Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial ... This is further explained at Gram staining § Orthographic note. Autochaperone Gram-variable and gram-indeterminate bacteria ... Bacteria are traditionally classified based on their Gram-staining response into the gram-positive and gram-negative bacteria. ... Thus, Gram staining cannot be reliably used to assess familial relationships of bacteria. Nevertheless, staining often gives ...
Gram-positive bacteria
Although bacteria are traditionally divided into two main groups, gram-positive and gram-negative, based on their Gram stain ... and are gram-indeterminate. The Deinococcota have gram-positive stains, although they are structurally similar to gram-negative ... The gram-positive and gram-negative staining response is also not a reliable characteristic as these two kinds of bacteria do ... In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally ...
Lutibacterium
... is a genus of Gram-negative staining bacteria. It includes the hydrocarbon-degrading strain Lutibacterium ...
Acetone
Engbaek, K; Johansen, KS; Jensen, ME (February 1979). "A new technique for Gram staining paraffin-embedded tissue". Journal of ... Acetone also used for destaining microscope slides of certain stains. Acetone is a weak Lewis base that forms adducts with soft ...
Thermomicrobia
There are some possible explanations of the inconsistency of Gram-staining result within the class. For Thermorudis ... Members of Thermomicrobia class have various Gram-staining results. Thermomicrobium roseum, Sphaerobacter thermophilus and ... Gram negative. Pleomorphic, non-motile, non-spore-forming rods. Non-sporulating. No diamino acid present. No peptidoglycan in ... Meanwhile, other members of Thermomicrobia class are reported to be Gram-positive and have typical monoderm cell envelope. ...
Sphingobacterium olei
S. olei is Gram-stain-negative, non-spore-forming, non-motile and rod-shaped. The cells are 0.5-0.6 µm in diameter and 1.1-1.3 ... Sphingobacterium olei is a Gram-stain-negative, rod-shaped, and non-motile bacterium. It was first isolated from oil- ... nov.: Glucose-Nonfermenting Gram-Negative Rods in CDC Groups IIK-2 and IIb". International Journal of Systematic Bacteriology. ...
Urethra
Investigations such as a gram stain of the discharge might reveal the cause; nucleic acid testing based on the first urine ...
Borrelia
... which classifies them as Gram-negative. However, this result is not easily visualized using Gram staining. They are typically ... Spirochetes can also be seen using Wright-stained blood smears. Parte AC (January 2014). "LPSN--list of prokaryotic names with ...
Lentilactobacillus hilgardii
On staining with Gram's iodine, the bacillus is Gram positive. L. hilgardii is considered a negative catalase (enzyme that ...
Diplorickettsia massiliensis
... centrifuge as revealed by subsequent staining. The isolate was Gram-negative when extracellular bacteria were stained.[citation ... Bacteria visualized in a rich culture established in XTC-2cell line by Gimenez staining appeared as intracellular red rods ... Diplorickettsia massiliensis species is an obligate intracellular, gram negative bacterium isolated from Ixodes ricinus ticks ... Negative staining showed that the bacteria have an average length of 1540 nm (range: 848 to 3067 nm) and an average diameter of ...
Differential staining
One commonly recognizable use of differential staining is the Gram stain. Gram staining uses two dyes: Crystal violet and ... Differential staining is a staining process which uses more than one chemical stain. Using multiple stains can better ... Acid-fast stains are also differential stains. http://www.uphs.upenn.edu/bugdrug/antibiotic_manual/Gram2.htm The Gram Stain ... Differential staining is used to detect abnormalities in the proportion of different white blood cells in the blood. The ...