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*  Ancestral reconstruction
... duplication or horizontal gene transfer. The "genome rearrangement problem", first posed by Watterson and colleagues, asks: ... Such techniques were used by Lemey and colleagues to geographically trace the ancestors of 192 Avian influenza A-H5N1 strains ... comparative genomics and ancestral genome reconstruction has been applied to identify ancient horizontal gene transfer events ... Count reconstructs the evolution of the size of gene families. EREM analyses the gain and loss of genetic features encoded by ...
*  Molecular phylogenetics
The most common approach is the comparison of homologous sequences for genes using sequence alignment techniques to identify ... The recent discovery of extensive horizontal gene transfer among organisms provides a significant complication to molecular ... Another application of the techniques that make this possible can be seen in the very limited field of human genetics, such as ... Modern sequence comparison techniques overcome this objection by the use of multiple sequences. Once the divergences between ...
*  Ligation (molecular biology)
... principles and techniques of Gene Transfer and Manipulation. Springer. ISBN 9781461597964. Robert W. Old; Sandy B. Primrose. ... The gene, once cloned into the cloning vector (called entry clone in this method), may be conveniently introduced into a ... This adenylyl group is then transferred to the phosphate group at the 5' end of a DNA chain, forming a DNA-adenylate complex. ... These methods allow cloning to be done much more rapidly, as well as allowing for simpler transfer of cloned DNA insert to ...
*  Streptomyces vinaceus
Microbial genetics applied to biotechnology principles and techniques of gene transfer and manipulation. London: Croom Helm. ...
*  Robert Sapolsky
Currently, he is working on gene transfer techniques to strengthen neurons against the disabling effects of glucocorticoids. ... as well as on the possibilities of gene therapy strategies for protecting susceptible neurons from disease. ...
*  Timeline of United States discoveries
Using gene transfer techniques, researchers in his lab inserted DNA from human bladder tumor cells into normal animal cells. ... 1986 Tumor suppressor gene A tumor suppressor gene, or anti-oncogene, is a gene that protects a cell from one step on the path ... This gene was found to belong to a sub-family of related genes, called ras, that was later discovered to play a role in causing ... When this gene is mutated to cause a loss or reduction in its function, the cell can progress to cancer, usually in combination ...
*  Elizabeth Nabel
She developed gene transfer techniques to investigate the pathophysiology of vascular cell growth and designed models of ... and for her gene transfer studies of the cardiovascular system. She set up a basic science lab to investigate the molecular ... Gene therapy for cardiovascular diseases. Circulation 1995;91:541-548. Ohno T, Gordon D, San H, Pompili VJ, Imperiale MJ, Nabel ... Gene therapy for vascular smooth muscle cell proliferation after arterial injury. Science 1994;265:781-784. Nabel EG, Yang Z-Y ...
*  Sperm-mediated gene transfer
... (SMGT) is a transgenic technique that transfers genes based on the ability of sperm cells to ... Transgenic animals have been produced successfully using gene transfer techniques such as sperm-mediated gene transfer. Though ... Gene therapy: the potential applicability of gene transfer technology to the human germline. International Journal of Medical ... Sperm-mediated gene transfer: applications and implications. BioEssays. 27(5):551-562. 5. Collares T, Campos VF, de Leon PM, ...
*  Waxy potato starch
A point of criticism of GMOs is the use of genetic engineering techniques to imprecisely perform horizontal gene transfer. For ... Two types of potato plant varieties are developed using different methods: one using traditional breeding techniques and ... the GM-amylopectin potato only potato DNA from other cultivars is transferred in as a transgene. Some organizations call ... another using genetic manipulation (GMO). Through traditional breeding techniques an amylose-free mutant was obtained without ...
*  BacMam
... which has contributed to its adoption as a standard gene transfer technique. The BacMam gene delivery technology is a transient ... Frederick M. Boyce, BacMam is a baculovirus-mediated gene transfer technique that has gained widespread use because of ... Bisafety and viral gene transfer vectors. In Biological safety, principles and practices, 3rd ed. D. O. Fleming and D. L. Hunt ... Baculovirus gene transfer into Mammalian cells, known from scientific research articles as BacMam, is the use of baculovirus to ...
*  Inder Verma
His development of virus mediated gene transfer techniques, including a stripped down version of HIV, has become the foundation ... He is recognized for seminal discoveries in the fields of cancer, immunology, and gene therapy. Verma was the editor-in-chief ... Verma is one of the foremost recognized leaders in gene therapy, retrovirology, and cancer. His work on viruses and cancer led ... American Society of Gene and Cell Therapy Outstanding Achievement Award 2008: Vilcek Prize in Biomedical Sciences 2008: AAISCR ...
*  Rafi Ahmed Kidwai Award
The Award was given to Dr Bansal for patenting a technique to transfer foreign gene to brinjal chloroplast. It was found that ...
*  Horizontal gene transfer
for a technique to decrease the impact of HGT events on maximum likelihood cladistical analyses. Horizontal Gene Transfer - A ... Citizendium:Horizontal gene transfer Citizendium:Horizontal gene transfer in prokaryotes Citizendium:Horizontal gene transfer ... Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between unicellular and/or ... Horizontal Gene Transfer at sciences.sdsu.edu Jain R, Rivera MC, Lake JA (March 1999). "Horizontal gene transfer among genomes ...
*  Vectors in gene therapy
This technique is referred to as oligonucleotide mediated gene repair, targeted gene repair, or targeted nucleotide alteration ... The most common use of lipoplexes has been in gene transfer into cancer cells, where the supplied genes have activated tumor ... Replication-defective vectors always contain a "transfer construct". The transfer construct carries the gene to be transduced ... This is mostly examined for gene transfer in the nervous system. The wild type HSV-1 virus is able to infect neurons and evade ...
*  Gene electrotransfer
Effective gene transfer to solid tumors using different nonviral gene delivery techniques: Electroporation, liposomes, and ... Gene electrotransfer is a versatile biotechnology technique that enables the transfer of genetic material into prokaryotic or ... Since gene electrotransfer is very simple, rapid and highly effective technique it first became very convenient replacement for ... Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982). Gene transfer into mouse lyoma cells by electroporation in high ...
*  Influenza research
A technique called reverse genetics allows scientists to manipulate the genomes of influenza viruses and to transfer genes ... "The rest of the genes were derived from an avirulent egg-adapted influenza A/PR/8/34 strain. The hemagglutinin gene was further ... CS1 maint: Multiple names: authors list (link) Sometimes a virus contains both avian adapted genes and human adapted genes. ... Another technique is use of cell cultures to grow vaccine strains; such as genetically engineering baculovirus to express a ...
*  Ralph L. Brinster
Brinster and longtime collaborator Richard Palmiter pioneered techniques to transfer foreign genes into mammals, and they ... During the 1960s, Brinster pioneered the development of techniques to manipulate mouse embryos-his techniques have made the ... These techniques have been conserved to the present day and form the foundation for all experimentation with the mammalian ... Their experiments showed that new genes could be, for the first time, introduced into the mammalian germline with the potential ...
*  Phenetics
Indeed, due to the effects of horizontal gene transfer, polyploid complexes and other peculiarities of plant genomics, phenetic ... Phenetic techniques include various forms of clustering and ordination. These are sophisticated ways of reducing the variation ... In addition, many of the techniques developed by phenetic taxonomists have been adopted and extended by community ecologists, ... techniques in botany - though less informative altogether - may, in these special cases, be less prone to errors compared with ...
*  Gene therapy for osteoarthritis
For this purpose, three techniques may be utilized: gene isolation, manipulations, and transferring to target cells. The most ... Ex vivo gene transfer and In-vivo gene transfer. One type of gene therapy in which the gene transfer takes place outside the ... However, in the field of OA gene therapy, researches has more focused on gene transfer as a delivery system for therapeutic ... While viral vectors are 40% more efficient in transferring genes, they are not fully appreciated for in vivo gene delivery ...
*  Conservative transposition
The Sleeping Beauty transposon system is an example of gene transfer system developed for use in vertebrates. Further ... This technique is used in transgenesis and insertional mutagenesis research fields. ... Current researchers have developed gene transfer systems on the basis of conservative transposition which can integrate new DNA ... The transposase coding region of these transposons is replaced by a gene of interest intended to be integrated into the genome ...
*  Genetically modified crops
Genetically engineered crops have genes added or removed using genetic engineering techniques, originally including gene guns, ... DNA transfers naturally between organisms. Several natural mechanisms allow gene flow across species. These occur in nature on ... The plasmid used to move the gene into soybeans was PV-GMGTO4. It contained three bacterial genes, two CP4 EPSPS genes, and a ... Bock, R. (2010). "The give-and-take of DNA: horizontal gene transfer in plants". Trends in Plant Science. 15 (1): 11-22. doi: ...
*  New Breeding Techniques
... using a technique called 'oligonucleotide-directed mutagenesis' (ODM)). Transferring a gene from an identical or closely- ... Whelan AI, Lema MA (2015). "Regulatory framework for gene editing and other new breeding techniques (NBTs) in Argentina". GM ... New Breeding Techniques (NBT), also named New Genetic Engineering Techniques, are a suite of genetic engineering methods that ... New breeding techniques (NBT)". New Techniques in Agricultural Biotechnology (PDF). Brussels: European Commission. pp. 56-70. ...
*  Timothy A Stewart
He was a pioneer in the technique of transferring recombinant genes to mice (transgenic mice) and in 1988 he and Philip Leder ...
*  Erwin Friedrich Wagner
... to develop techniques allowing the micro-injection of DNA into fertilized eggs as well as the development of gene transfer ... Erwin Wagner's studies are focused in the area of gene function in both healthy and pathological states. He is a leader in the ... Wagner obtained his PhD with a thesis on "Regulation of gene expression after virus infection" in 1978. In 1979, Erwin Wagner ...
*  Mitochondrial replacement therapy
Despite the promising outcomes of the two techniques, pronuclear transfer and spindle transfer, mitochondrial gene replacement ... This technique is used in cases when mothers carry genes for mitochondrial diseases. The two most common techniques in ... As of 2016 there were three MRT techniques: maternal spindle transfer (MST), pronuclear transfer (PNT), and more recently as of ... In 2016, John Zhang and his team at New Hope Fertility Center in New York, USA used the spindle transfer technique to help a ...
*  Health realization
There are no steps, no uniformly appropriate internal attitudes, and no techniques within it. The "health of the helper" is ... a change process developed by philosopher Eugene Gendlin, supports the theory that progress in psychotherapy is dependent on ... that goes beyond a simple transfer of information. ... accessible over the lifespan without stress-relief techniques? ... Health Realization ("HR"), like Sydney Banks's philosophy, is deliberately not taught as a set of "techniques" but as an ...
*  Spinocerebellar ataxia
Finally, another gene transfer technology discovered in 2011 has also been shown by Dr. Davidson to hold great promise and ... Pedro Gonzalez-Alegre are currently working to move this technique into a Phase 1 clinical trial. ... later genes were called SCA2, SCA3, etc. Usually, the "type" number of "SCA" refers to the order in which the gene was found. ... In many cases people are not aware that they carry a relevant gene until they have children who begin to show signs of having ...