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*  Directed evolution
The first step in performing a cycle of directed evolution is the generation of a library of variant genes. The sequence space ... It consists of subjecting a gene to iterative rounds of mutagenesis (creating a library of variants), selection (expressing the ... A focussed library contains fewer variants than a traditional random mutagenesis library and so does not require such high- ... Even if an organism is used to express the gene of interest, by mutagenising only that gene, the rest of the organism's genome ...
*  DNA shuffling
It is used to rapidly increase DNA library size. First, DNase is used to fragment a set of parent genes into pieces of 50-100 ... after some of the DNA molecules reach the size of the parental genes. These genes can then be amplified with another PCR, this ... Restriction enzymes that cut in similar places are used to digest members of the gene family DNA fragments are joined together ... It is possible to recombine portions of these genes to generate hybrids or chimeric forms with unique properties, hence the ...
*  Molecular cloning
This "germ line gene therapy" is considered by many to be unethical in human beings. The second type of gene therapy, "somatic ... The total population of individual clones obtained in a molecular cloning experiment is often termed a DNA library. Libraries ... Gene therapy involves supplying a functional gene to cells lacking that function, with the aim of correcting a genetic disorder ... August JT (1997). Gene Therapy. 40. Academic Press. p. 508. ISBN 978-0-08-058132-3. Pfeifer A, Verma IM (2001). "Gene therapy: ...
*  Eugene Public Library
... Archived from the original (PDF) on April 1, 2010. "About the library." Eugene Public Library. Retrieved ... The Eugene Public Library is a municipal public library in Eugene, Oregon, United States. It has been in four different ... "A Rich History". Eugene Library Foundation. Retrieved April 20, 2014. Lowry, Marian (August 20, 1931). "Eugene Public Library ... Eugene Public Library was established as a tax-supported entity in 1904. In 1906, Oregon's first Carnegie Library was ...
*  Structure-based combinatorial protein engineering
To create the corresponding library of genes, the breeding scheme of Gregor Mendel was adapted into a PCR strategy to ... These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or ... O'Maille PE, Tsai MD, Greenhagen BT, Chappell J, Noel JP (2004). "Gene library synthesis by structure-based combinatorial ... Structure-based combinatorial protein engineering (SCOPE) is a synthetic biology technique for creating gene libraries ( ...
*  DNA sequencing
Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD (2006). "Amplification of complex gene libraries by ... DNA sequencing may be used to determine the sequence of individual genes, larger genetic regions (i.e. clusters of genes or ... Min Jou W, Haegeman G, Ysebaert M, Fiers W (May 1972). "Nucleotide sequence of the gene coding for the bacteriophage MS2 coat ... The major landmark of RNA sequencing is the sequence of the first complete gene and the complete genome of Bacteriophage MS2, ...
*  Cambridge Antibody Technology
Human antibodies from V-gene libraries displayed on phage". Journal of Molecular Biology. 222 (3): 581-97. doi:10.1016/0022- ... Free Online Library". Thefreelibrary.com. Retrieved 2010-05-12. Foley, Stephen (27 March 2003). "CAT's bid hopes floored by ... Free Online Library". Thefreelibrary.com. 2004-02-01. Retrieved 2010-05-12. "Amgen - Media - Press Release". Xt.amgen.com. ... This should make it possible to construct libraries that are orders of magnitude larger than those created using phage display ...
*  No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing
Quan, Jiayuan; Tian, Jingdong (2009-01-01). "Circular polymerase extension cloning of complex gene libraries and pathways". ... the tetR gene, which codes for tetracycline repressor protein, under control of a constitutive promoter; and the cmr gene for ... Because the system uses a plasmid that expresses the exo and gam genes from the λ-red system it is able to use oligonucleotide ... The first plasmid used in the pioneering work of Reisch and Prather is composed of: the cas9 gene under the control of the PTET ...
*  Protein engineering
This results in a library of chimeric genes in which contribution of genes to 5' and 3' end will be reversed as compared to the ... Whole-gene library synthesis is replacing shuffling and mutagenesis protocols for library preparation. Also highly specific low ... The first with gene A on the N-terminus. And the other having gene B on the N-terminus. These hybrid gene fragments are ... Asexual methods no not generate any cross links between parental genes. Single genes are used to create mutant libraries using ...
*  Single-domain antibody
A different method uses gene libraries from animals that have not been immunized beforehand. Such naïve libraries usually ... The process is similar, comprising gene libraries from immunized or naïve donors and display techniques for identification of ... By reverse transcription and polymerase chain reaction, a gene library of single-domain antibodies containing several million ... Human single-domain VHs can be isolated from engineered antibody domain libraries by phage display. Human single-domain ...
*  16S ribosomal RNA
"Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end ... The genes coding for it are referred to as 16S rRNA gene and are used in reconstructing phylogenies, due to the slow rates of ... The 16S rRNA gene is used as the standard for classification and identification of microbes, because it is present in most ... The bacterial 16S gene contains nine hypervariable regions (V1-V9) ranging from about 30-100 base pairs long that are involved ...
*  Gene Honda
"DigiGuide Library: Gene Honda". Digi Guide.com. Retrieved 2010-05-14. ... Eugene "Gene" Honda is a popular public address announcer for the Chicago White Sox (starting in 1985, full-time since 1991), ... Gene Honda also is a teacher for the After School Matters (ASM) Sports Broadcasting program at Curie High School for the past ... Gene graduated from Chicago's Senn High School in 1972. Honda attended the University of Illinois and is part of the class of ...
*  Prince Eugene of Savoy
Eugene's library, prints and drawings were purchased by the Emperor in 1737 and have since passed into Austrian national ... McKay: Prince Eugene of Savoy, p. 193 Eugene had purchased this land in 1726. McKay: Prince Eugene of Savoy, p. 195. Maria ... McKay: Prince Eugene of Savoy, 177 McKay: Prince Eugene of Savoy, 180. Eugene was reluctant to leave his palaces and friends: ... Prince Eugene: Twin Marshal with Marlborough, 32 McKay: Prince Eugene of Savoy, 22 MacMunn: Prince Eugene: Twin Marshal with ...
*  Immunoscreening
Clones are screened for the presence of the gene product (a protein). This strategy requires first that a gene library is ... Immunoscreening is a method of biotechnology to detect a polypeptide produced from a cloned gene. ...
*  Untitled (Hardy)
It was administered the Cultural Services Division of the City of Eugene's Library, Recreation and Cultural Services Department ... Untitled is an outdoor 1952 fountain and sculpture by Tom Hardy, installed at the Park Blocks in Eugene, Oregon, United States ... Tom Hardy's untitled fountain (1952) is installed at the northeast corner of Eugene's Park Blocks, near the intersection of 8th ... List of public art in Eugene, Oregon Running Horses (1986) by Tom Hardy (Portland, Oregon) "Untitled, (sculpture)". Smithsonian ...
*  Fungal Genetics Stock Center
In the 1980s and 1990s the FGSC added molecular materials including cloned genes, cloning vectors and gene libraries to the ... Since adding targeted gene deletion strains of Neurospora crassa, Cryptococcus neoformans and Candida albicans to the ... and accessioned over 13,000 gene deletion mutants together comprising a whole genome deletion set between 2006 and 2014. ... His research using gene and genome sequence has led to new insights into mutation, and the nature of genome variability among ...
*  Knockout mouse
The gene to be knocked out is isolated from a mouse gene library. Then a new DNA sequence is engineered which is very similar ... Knocking out the activity of a gene provides information about what that gene normally does. Humans share many genes with mice ... Usually, the new sequence is also given a marker gene, a gene that normal mice don't have and that confers resistance to a ... At times, loss of activity during development may mask the role of the gene in the adult state, especially if the gene is ...
*  Linke-Hofmann R.I
ISBN 978-0-370-00103-6. Image of the Celon, covered aircraft, 8/15., University of Dallas Eugene McDermott Library.. ...
*  Joseph Lane
University of Oregon Libraries, Eugene, OR, 2005. "Notable Oregonians: Joseph Lane - Governor". Oregon Blue Book. Oregon ...
*  Eugene Manlove Rhodes
The library's Eugene Manlove Rhodes Room houses this collection and the library's other Southwest books. Good men and true. ... Works by Eugene Manlove Rhodes at Project Gutenberg Works by or about Eugene Manlove Rhodes at Internet Archive Eugene Manlove ... Eugene Manlove Rhodes (January 19, 1869 - June 27, 1934) was a writer who was nicknamed the "cowboy chronicler". Rhodes was ... by J. Frank Dobie, 1949 Sunset Land, 1955 Bar Cross man; the life & personal writings of Eugene Manlove Rhodes [by] W.H. ...
*  Chris Rogers (mathematician)
"Leonard ("Chris") Christopher Gordon Rogers". Cornell University Library, Eugene B. Eynkin Collection of Mathematica Interviews ...
*  Eugene C. Eppley Administration Building
"University of Omaha Library To Bear Name of Donor, 'Gene Eppley Library'". The Gateway. University of Nebraska Omaha. December ... and in 1976 the original Eugene Eppley Library became the current Eppley Administration building. Eppley's gift and the ... Milo told him that a library was in order for their new campus, to which Eppley wrote a check for $850,000, the entire cost of ... The Eugene C. Eppley Administration Building is located on the University of Nebraska at Omaha north campus in Omaha, Nebraska ...
*  Subcloning
Therefore, successfully transformed bacteria would be "selected." In this example, a gene from mammalian gene library will be ... Typical marker genes are for antibiotic resistance or nutrient biosynthesis. So, for example, the "marker gene" could be for ... If the bacteria that were supposed to pick up the desired plasmid had picked up the desired gene then they would also contain ... Restriction enzymes are used to excise the gene of interest (the insert) from the parent. The insert is purified in order to ...
*  Physcomitrella patens
Physcomitrella knockout mutants: Deviating phenotypes induced by transformation with a gene-disruption-library. Physcomitrella ... The PpMsh2 gene is necessary in P. patens to preserve genome integrity. Genes Ppmre11 and Pprad50 of P. patens encode ... "High frequency of phenotypic deviations in Physcomitrella patens plants transformed with a gene-disruption library". BMC Plant ... The targeted deletion or alteration of moss genes relies on the integration of a short DNA strand at a defined position in the ...
*  Prince Lucien Campbell
University of Oregon Libraries, Eugene, Oregon. Retrieved April 8, 2016. "The University of Oregon and the Cemetery" (PDF). ... Eugene. August 15, 1925. Retrieved April 8, 2016. "2011 - Campbell Hall". 2011. Retrieved April 10, 2016. "150 years of ... the Eugene Guard carried an editorial page tribute to Campbell, which said in part: He was unusual... Nature had given him the ... Eugene Masonic Cemetery. Retrieved April 13, 2016. "Presidential History". Retrieved April 8, 2016. "Past presidents at the U ...
*  UCKL1
1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1-2): 149- ... Uridine-cytidine kinase-like 1 is an enzyme that in humans is encoded by the UCKL1 gene. GRCh38: Ensembl release 89: ... 2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome ... "Entrez Gene: UCKL1 uridine-cytidine kinase 1-like 1". Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based ...
*  SeSaM-Biotech GmbH
These mutagenesis methods are applied to generate gene libraries for customers or are used in comprehensive protein engineering ... strategy and gene library generation using its patented platform technology Sequence Saturation Mutagenesis. The SeSaM-Biotech ... Starting from SeSaM library generation, SeSaM-Biotech now applies a variety of random and focused mutagenesis methods in ... By non-specific introduction of universal or degenerate bases at every position in the gene sequence, SeSaM overcomes the ...